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The map label for this gene is pecT [H]

Identifier: 87200490

GI number: 87200490

Start: 2678583

End: 2679455

Strand: Direct

Name: pecT [H]

Synonym: Saro_2477

Alternate gene names: 87200490

Gene position: 2678583-2679455 (Clockwise)

Preceding gene: 87200487

Following gene: 87200491

Centisome position: 75.21

GC content: 65.98

Gene sequence:

>873_bases
ATGGCCCTGCGTAATCTTGATGTCGATCTGCTCCGCTGCTTCGTAACCATCGCGGATACCGGCAGCTTCACGCGGGCCGG
GGAACGGCTGGGCCGGACGCAATCGGCCATTTCGCTGCAGCTGAAGCGACTGGAGGAACAGCTCGGCCGAACGCTCTTCG
TGCGCAGTCCGCGCAGTTTGGCGATCACGCCCAATGGCGAAAGGCTGATCGGGCCTGCGCGCCAGATCCTGCGCCTGCAC
GATGCCACCGTGGCCGAGATGTTCGAACCGGACATCGCCGGGTCGGTGCGCATCGGCGTGCCGGAGGATTTTGCAACGGC
GCACCTTCCCTCGGTGCTCGCAGCCTTTGCCGAAGCGCATCCGCTGGTCGAACTGGAAGTGACCTGCGACCTCACGCTCA
ACCTGCTCGAACGCTTTCATGCCGGGCATTTCGATCTCGTGCTGGTCAAGCGCCAGCCCACGCAGGAGCGACACGAGGGC
GTGCGCGTCTGGCGCGAACCGCTGGTTTGGGTGGCACGCGACCGAAAGTCCGCCGTCGACTATTCGCGGGTGCCGCTGGT
GGTCTCGCCCGAACCCTGCGTCTATCGCAAGCGCGCGACTGAGGCGCTGGACGCTATGGGGCGGCCGTGGCGCACCGCCT
ATACCTCAACCAGCCTTGCTGGCAGTCTTTCCGCTGTGCGGGCCGGACTTGGGATCACGGTTCTGCCGCTTGAGATGGTC
CCGTCCTCCCTGACGGCCGTGGGCCCCGAGGCGGGCCTGCCGGCGCTATACGATACCGAAATCGCACTGATAGAGGCCGC
TGGCCTTTCAGATACCGCACACCGCCTCGCACAGCACATCATCGCAGCGCTCGAGCGGGGGCGCGGCACCTGA

Upstream 100 bases:

>100_bases
GCGGGGATGTCTCGGGGTGCAGGTGCGCCACGATTCATGATTTTGGAAACGAATATCTGAAATATTGAAGATTGCATTTT
GAAATGATAAATCGGCCCGT

Downstream 100 bases:

>100_bases
AGCCCAATCGTCGCTGGGCTGACTGCACAGGGCATCGCGCCCATTGTTGGCGTGTCCGACCGATACGTGCGCGCGATCCG
CGACGCGTAACGCGTTGCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MALRNLDVDLLRCFVTIADTGSFTRAGERLGRTQSAISLQLKRLEEQLGRTLFVRSPRSLAITPNGERLIGPARQILRLH
DATVAEMFEPDIAGSVRIGVPEDFATAHLPSVLAAFAEAHPLVELEVTCDLTLNLLERFHAGHFDLVLVKRQPTQERHEG
VRVWREPLVWVARDRKSAVDYSRVPLVVSPEPCVYRKRATEALDAMGRPWRTAYTSTSLAGSLSAVRAGLGITVLPLEMV
PSSLTAVGPEAGLPALYDTEIALIEAAGLSDTAHRLAQHIIAALERGRGT

Sequences:

>Translated_290_residues
MALRNLDVDLLRCFVTIADTGSFTRAGERLGRTQSAISLQLKRLEEQLGRTLFVRSPRSLAITPNGERLIGPARQILRLH
DATVAEMFEPDIAGSVRIGVPEDFATAHLPSVLAAFAEAHPLVELEVTCDLTLNLLERFHAGHFDLVLVKRQPTQERHEG
VRVWREPLVWVARDRKSAVDYSRVPLVVSPEPCVYRKRATEALDAMGRPWRTAYTSTSLAGSLSAVRAGLGITVLPLEMV
PSSLTAVGPEAGLPALYDTEIALIEAAGLSDTAHRLAQHIIAALERGRGT
>Mature_289_residues
ALRNLDVDLLRCFVTIADTGSFTRAGERLGRTQSAISLQLKRLEEQLGRTLFVRSPRSLAITPNGERLIGPARQILRLHD
ATVAEMFEPDIAGSVRIGVPEDFATAHLPSVLAAFAEAHPLVELEVTCDLTLNLLERFHAGHFDLVLVKRQPTQERHEGV
RVWREPLVWVARDRKSAVDYSRVPLVVSPEPCVYRKRATEALDAMGRPWRTAYTSTSLAGSLSAVRAGLGITVLPLEMVP
SSLTAVGPEAGLPALYDTEIALIEAAGLSDTAHRLAQHIIAALERGRGT

Specific function: Regulates pectinase gene expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=281, Percent_Identity=32.7402135231317, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787806, Length=243, Percent_Identity=26.3374485596708, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693105, Length=239, Percent_Identity=25.5230125523013, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787589, Length=141, Percent_Identity=37.5886524822695, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=28.0487804878049, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786713, Length=146, Percent_Identity=27.3972602739726, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786401, Length=131, Percent_Identity=35.1145038167939, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=151, Percent_Identity=28.476821192053, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994962, Length=151, Percent_Identity=27.8145695364238, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787601, Length=249, Percent_Identity=23.2931726907631, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=142, Percent_Identity=30.9859154929577, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=143, Percent_Identity=30.7692307692308, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=147, Percent_Identity=27.2108843537415, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI2367136, Length=137, Percent_Identity=32.1167883211679, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=27.7551020408163, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082132, Length=232, Percent_Identity=22.8448275862069, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31703; Mature: 31572

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRNLDVDLLRCFVTIADTGSFTRAGERLGRTQSAISLQLKRLEEQLGRTLFVRSPRSL
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCEE
AITPNGERLIGPARQILRLHDATVAEMFEPDIAGSVRIGVPEDFATAHLPSVLAAFAEAH
EECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCC
PLVELEVTCDLTLNLLERFHAGHFDLVLVKRQPTQERHEGVRVWREPLVWVARDRKSAVD
CCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEECCCHHCCC
YSRVPLVVSPEPCVYRKRATEALDAMGRPWRTAYTSTSLAGSLSAVRAGLGITVLPLEMV
CCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEHHHC
PSSLTAVGPEAGLPALYDTEIALIEAAGLSDTAHRLAQHIIAALERGRGT
CCHHHHCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALRNLDVDLLRCFVTIADTGSFTRAGERLGRTQSAISLQLKRLEEQLGRTLFVRSPRSL
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCEE
AITPNGERLIGPARQILRLHDATVAEMFEPDIAGSVRIGVPEDFATAHLPSVLAAFAEAH
EECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCC
PLVELEVTCDLTLNLLERFHAGHFDLVLVKRQPTQERHEGVRVWREPLVWVARDRKSAVD
CCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEECCCHHCCC
YSRVPLVVSPEPCVYRKRATEALDAMGRPWRTAYTSTSLAGSLSAVRAGLGITVLPLEMV
CCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEHHHC
PSSLTAVGPEAGLPALYDTEIALIEAAGLSDTAHRLAQHIIAALERGRGT
CCHHHHCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]