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The map label for this gene is ndhF [H]

Identifier: 87200489

GI number: 87200489

Start: 2677081

End: 2678460

Strand: Reverse

Name: ndhF [H]

Synonym: Saro_2476

Alternate gene names: 87200489

Gene position: 2678460-2677081 (Counterclockwise)

Preceding gene: 87200492

Following gene: 87200488

Centisome position: 75.2

GC content: 67.17

Gene sequence:

>1380_bases
ATGAGCCAGACGACCGTCAACGCAACCGCAGTTCCCTCCCGAACGCCTGTCATTCGTGCACTGTCATTTTCGCGTGCCGA
TCTTCCGGTGCTTGCACTCGGCATGCTCCTTGCTGCTGCAATGGCCGATGGCCACGCGGTTCTGACCACAGCGGGCGTGG
CCCTGGCAGGGTTGTCTTTGCTGGTGACCGTTGTCGTGCTGGTGTTCGCGCGTCGCCAACTGCGCGCCGATCCGCGCGCG
GGGGCGTTTGCCCGCATGGCCGTGGGCCTTGCTCTTGCCACGATGCTGATGGGCCTTGCCGCCGATGCGCTCCTGCTGGC
GGCGGCGTGGATCGTGACCGGGCGGCTGATGGCTGGAATGATCGGCCATGTAGGCGACTCGGGAGAAGCCCGCTCGGCCG
CACGGCGTGCGAGCCTGTCTTTCGCGGTGAGCGATCTGGCGCTGGTGACCGCGGTCCTGTTTCTGGCGATCGGCGCGAAA
TCCACCGGGATCGCCGCGATCGGCGCAGCAGTGCCCACCATGGCCGTCGGGTACCTGATGCCGGCGGCATTCCTGCTTGC
CATCGCCGCACTGGCGCGCTGTGCCTTGCCGCCGTTCTCGACCTGGCTGATGCGTTCGCTTGCCGCGCCAACGCCGGTGT
CGGCCCTAATGCATGCGGGCTTCGTCAACGCCGGCGGCTTTCTGATGGTCCGCTTCGCGCCGGTTATCGAAGCGGCCACG
GCGGCCCGGTTCATGCTCTTCACCGTCGGGATCATGGCTGCGCTGTTCGGCAGTGCGATCATGCTCGTCCGCAACGACGT
GAAAGGTTCGCTGGCTGCATCGACCGTCTCCCAGATGGGCTTCATGCTGCTGACTGTGGCGTTGGGCGCTTATGCCGCAG
CATTGTGGCACATGGTGGCGCATGGGGTGTTCAAGGCCTGGCTGTTCCTTGGTTCGGCAGGCACCGTCTCGAGCGCCCCA
GCCCGCGCCAAGGCGTTGCCCCAGCCCTGGCCCGCCGCGATTGCGCTTGTCACCATGGTCGGCGCGTACGCGTTGATCCA
GACGGGCGAAGGAGGCGTGCTGCTCCCGGTAGGCCTGGCCTGCACCGCGCTGCTCAGCGCGCTGACGGTCGGCCTGCGGT
CCGTTTCGCGCAGCGCGGTGGGGCTCGTGGTGGCCGCAGTTCCGGTGATGCTGATCGCGCTGAATGCGGCTGCGCTGGCG
GTGATGTCGGCCTTGCAGGACAATGCCGCGCTGCCCTTGATCCACGATTACGCGCAGTTCGCCCTGCTCTCGCTGTTTCT
GGCGGGATGGGTGGCCCAGCAGCGCGTCGCCAGTGGCGAGCGCAACCTGCCACCAGCGCTGTTCGCCCGCCTGTTCCATG
CCGGAAATCTCTCGGCCTGA

Upstream 100 bases:

>100_bases
AATGCAATCTTCAATATTTCAGATATTCGTTTCCAAAATCATGAATCGTGGCGCACCTGCACCCCGAGACATCCCCGCCG
AGGGGCACTTGGAGCCAGAG

Downstream 100 bases:

>100_bases
CCTCTGCAATTTCCAGCTCTCAAGGACGCCATGCCATGACCCTCGCGACCAGAAGCCTCATCAAGAACCTCTCCTCCGCA
CTGGGCGCCCAGGCCGAGCG

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSQTTVNATAVPSRTPVIRALSFSRADLPVLALGMLLAAAMADGHAVLTTAGVALAGLSLLVTVVVLVFARRQLRADPRA
GAFARMAVGLALATMLMGLAADALLLAAAWIVTGRLMAGMIGHVGDSGEARSAARRASLSFAVSDLALVTAVLFLAIGAK
STGIAAIGAAVPTMAVGYLMPAAFLLAIAALARCALPPFSTWLMRSLAAPTPVSALMHAGFVNAGGFLMVRFAPVIEAAT
AARFMLFTVGIMAALFGSAIMLVRNDVKGSLAASTVSQMGFMLLTVALGAYAAALWHMVAHGVFKAWLFLGSAGTVSSAP
ARAKALPQPWPAAIALVTMVGAYALIQTGEGGVLLPVGLACTALLSALTVGLRSVSRSAVGLVVAAVPVMLIALNAAALA
VMSALQDNAALPLIHDYAQFALLSLFLAGWVAQQRVASGERNLPPALFARLFHAGNLSA

Sequences:

>Translated_459_residues
MSQTTVNATAVPSRTPVIRALSFSRADLPVLALGMLLAAAMADGHAVLTTAGVALAGLSLLVTVVVLVFARRQLRADPRA
GAFARMAVGLALATMLMGLAADALLLAAAWIVTGRLMAGMIGHVGDSGEARSAARRASLSFAVSDLALVTAVLFLAIGAK
STGIAAIGAAVPTMAVGYLMPAAFLLAIAALARCALPPFSTWLMRSLAAPTPVSALMHAGFVNAGGFLMVRFAPVIEAAT
AARFMLFTVGIMAALFGSAIMLVRNDVKGSLAASTVSQMGFMLLTVALGAYAAALWHMVAHGVFKAWLFLGSAGTVSSAP
ARAKALPQPWPAAIALVTMVGAYALIQTGEGGVLLPVGLACTALLSALTVGLRSVSRSAVGLVVAAVPVMLIALNAAALA
VMSALQDNAALPLIHDYAQFALLSLFLAGWVAQQRVASGERNLPPALFARLFHAGNLSA
>Mature_458_residues
SQTTVNATAVPSRTPVIRALSFSRADLPVLALGMLLAAAMADGHAVLTTAGVALAGLSLLVTVVVLVFARRQLRADPRAG
AFARMAVGLALATMLMGLAADALLLAAAWIVTGRLMAGMIGHVGDSGEARSAARRASLSFAVSDLALVTAVLFLAIGAKS
TGIAAIGAAVPTMAVGYLMPAAFLLAIAALARCALPPFSTWLMRSLAAPTPVSALMHAGFVNAGGFLMVRFAPVIEAATA
ARFMLFTVGIMAALFGSAIMLVRNDVKGSLAASTVSQMGFMLLTVALGAYAAALWHMVAHGVFKAWLFLGSAGTVSSAPA
RAKALPQPWPAAIALVTMVGAYALIQTGEGGVLLPVGLACTALLSALTVGLRSVSRSAVGLVVAAVPVMLIALNAAALAV
MSALQDNAALPLIHDYAQFALLSLFLAGWVAQQRVASGERNLPPALFARLFHAGNLSA

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=265, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1788614, Length=276, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46656; Mature: 46525

Theoretical pI: Translated: 11.75; Mature: 11.75

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTTVNATAVPSRTPVIRALSFSRADLPVLALGMLLAAAMADGHAVLTTAGVALAGLSL
CCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVTVVVLVFARRQLRADPRAGAFARMAVGLALATMLMGLAADALLLAAAWIVTGRLMAGM
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGHVGDSGEARSAARRASLSFAVSDLALVTAVLFLAIGAKSTGIAAIGAAVPTMAVGYLM
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
PAAFLLAIAALARCALPPFSTWLMRSLAAPTPVSALMHAGFVNAGGFLMVRFAPVIEAAT
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHH
AARFMLFTVGIMAALFGSAIMLVRNDVKGSLAASTVSQMGFMLLTVALGAYAAALWHMVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGVFKAWLFLGSAGTVSSAPARAKALPQPWPAAIALVTMVGAYALIQTGEGGVLLPVGLA
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEHHHHH
CTALLSALTVGLRSVSRSAVGLVVAAVPVMLIALNAAALAVMSALQDNAALPLIHDYAQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALLSLFLAGWVAQQRVASGERNLPPALFARLFHAGNLSA
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQTTVNATAVPSRTPVIRALSFSRADLPVLALGMLLAAAMADGHAVLTTAGVALAGLSL
CCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVTVVVLVFARRQLRADPRAGAFARMAVGLALATMLMGLAADALLLAAAWIVTGRLMAGM
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGHVGDSGEARSAARRASLSFAVSDLALVTAVLFLAIGAKSTGIAAIGAAVPTMAVGYLM
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
PAAFLLAIAALARCALPPFSTWLMRSLAAPTPVSALMHAGFVNAGGFLMVRFAPVIEAAT
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHH
AARFMLFTVGIMAALFGSAIMLVRNDVKGSLAASTVSQMGFMLLTVALGAYAAALWHMVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HGVFKAWLFLGSAGTVSSAPARAKALPQPWPAAIALVTMVGAYALIQTGEGGVLLPVGLA
HHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHEEECCCCEEEHHHHH
CTALLSALTVGLRSVSRSAVGLVVAAVPVMLIALNAAALAVMSALQDNAALPLIHDYAQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ALLSLFLAGWVAQQRVASGERNLPPALFARLFHAGNLSA
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]