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The map label for this gene is tfdFI [H]

Identifier: 87200468

GI number: 87200468

Start: 2646384

End: 2647562

Strand: Reverse

Name: tfdFI [H]

Synonym: Saro_2455

Alternate gene names: 87200468

Gene position: 2647562-2646384 (Counterclockwise)

Preceding gene: 87200470

Following gene: 87200467

Centisome position: 74.34

GC content: 64.89

Gene sequence:

>1179_bases
GTGGGCAGCGTCAGAGCCAGCGAGATGACGTTTACCGAAACCGAGCGGGTCACCATGGGGCGTCCTGCCCGCGAGGCGAT
CCGTGACCAGGCGGAGAAGATGGGGGCGCGCCGCGTGTTCATCCTCGCTTCGGACACCTTGCGGACCAAAACCGACGAGA
TCGCAGCGATCGAGCGCGAACTTGGTGAACGGCACGCTGCGACCTTCAGCGGCATTGCGCCCCATGCACCCCGATCGGAC
GTCCTGCGCGCCGCCGATGCCGCACGCGCGGCTGGCGCGGATCTGATCGTCTCGGTGGGTGGTGGCTCCGTCACCGACAG
CGCGAAGATCGTGGCGCTGCTGCTCAAGCACGATGTGCGCAGCGTCGAGGCGTTCGAGCCGCTGCACGTCTATGTCGACG
GCGATGGCAATGTGATCAACCCGCTCGCCGTCGGCCCGGATGTGCGTGTCATATGTGTGCCGACCACGCTTTCCGGCGGC
GAGTTCAACGCGCTTTCAGGCGCTACCGACGAAGCGGTGCAGCACAAGCAGGGCTACGAGCATCGCGACATGGCGCCGGT
CATGGTTGTGCTGGACCCGGCGCTGACGGTCTACACCCCCGAATGGCTCTGGCTTTCGACCGGGGTCCGATCTGTCGACC
ATGCGGTGGAGACGCTCGCCTCCCACCTTTCCAATGATTTTGCGGATGGCCTTGCGGACAGCGCCCTGCGCCTTCTGGTC
GAAGGGCTCAGTCGTGCCAAGGCGGACCCGACAGACATGGAGGCGCGCCTGAAGTGCCAGATCGGTGCATGGCAATCGAT
GATCTCGATCATCGGCGGCGTGCCGATGGGCGCCAGCCATGCCATCGGCCACATCCTCGGCGGCACTTGCGACGTGCCGC
ACGGCTATACGTCGTGCGTCATGTCCCCCTATGTGCTGGCCTGGAATGCCGAGCATGACGCGAGCCGGCAGGGGCGCATT
CTGCAGTGCCTCGGCAATACCCACGCTTCGGCGTCCGAAGCGCTTGACGCCTTCATCCGCAGCCTGGGCATGCCTCGCAC
CCTTTCCGAAGTGGGCGTCGGCGAGGACCGCTTCCAGCAGGTAGCAGAATATACCATGCTTGATATATGGGGGAGGACCA
ATCCGCGGCCGGTCCGTTCGCCCGCTGATATCCTGGAGATACTGAGAAAGGCCGCATGA

Upstream 100 bases:

>100_bases
ATCACAGGTCAAAAAATATACTAGCGGGTAATGCGTTGTCGTGGCACTCTCTGTGCAAGCGCCTCCCAGCAAGCGAAAGG
CGCGTGGAAAGGAGAAAGTC

Downstream 100 bases:

>100_bases
CCAAGTCCAGCACCGCCGCCAAGCCGCGTCAACGTGCCGCCGGCGCGCCTACCGGGCGCGAGATCAAGCCCGGTGTCGTC
GCGTTCAAGCGGGCCCGCAT

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: Maleylacetate reductase I [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MGSVRASEMTFTETERVTMGRPAREAIRDQAEKMGARRVFILASDTLRTKTDEIAAIERELGERHAATFSGIAPHAPRSD
VLRAADAARAAGADLIVSVGGGSVTDSAKIVALLLKHDVRSVEAFEPLHVYVDGDGNVINPLAVGPDVRVICVPTTLSGG
EFNALSGATDEAVQHKQGYEHRDMAPVMVVLDPALTVYTPEWLWLSTGVRSVDHAVETLASHLSNDFADGLADSALRLLV
EGLSRAKADPTDMEARLKCQIGAWQSMISIIGGVPMGASHAIGHILGGTCDVPHGYTSCVMSPYVLAWNAEHDASRQGRI
LQCLGNTHASASEALDAFIRSLGMPRTLSEVGVGEDRFQQVAEYTMLDIWGRTNPRPVRSPADILEILRKAA

Sequences:

>Translated_392_residues
MGSVRASEMTFTETERVTMGRPAREAIRDQAEKMGARRVFILASDTLRTKTDEIAAIERELGERHAATFSGIAPHAPRSD
VLRAADAARAAGADLIVSVGGGSVTDSAKIVALLLKHDVRSVEAFEPLHVYVDGDGNVINPLAVGPDVRVICVPTTLSGG
EFNALSGATDEAVQHKQGYEHRDMAPVMVVLDPALTVYTPEWLWLSTGVRSVDHAVETLASHLSNDFADGLADSALRLLV
EGLSRAKADPTDMEARLKCQIGAWQSMISIIGGVPMGASHAIGHILGGTCDVPHGYTSCVMSPYVLAWNAEHDASRQGRI
LQCLGNTHASASEALDAFIRSLGMPRTLSEVGVGEDRFQQVAEYTMLDIWGRTNPRPVRSPADILEILRKAA
>Mature_391_residues
GSVRASEMTFTETERVTMGRPAREAIRDQAEKMGARRVFILASDTLRTKTDEIAAIERELGERHAATFSGIAPHAPRSDV
LRAADAARAAGADLIVSVGGGSVTDSAKIVALLLKHDVRSVEAFEPLHVYVDGDGNVINPLAVGPDVRVICVPTTLSGGE
FNALSGATDEAVQHKQGYEHRDMAPVMVVLDPALTVYTPEWLWLSTGVRSVDHAVETLASHLSNDFADGLADSALRLLVE
GLSRAKADPTDMEARLKCQIGAWQSMISIIGGVPMGASHAIGHILGGTCDVPHGYTSCVMSPYVLAWNAEHDASRQGRIL
QCLGNTHASASEALDAFIRSLGMPRTLSEVGVGEDRFQQVAEYTMLDIWGRTNPRPVRSPADILEILRKAA

Specific function: May Act On The Acetaldehyde Produced From The Degradation Of Ethanolamine. [C]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082107, Length=373, Percent_Identity=25.201072386059, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI48994951, Length=355, Percent_Identity=25.6338028169014, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789163, Length=402, Percent_Identity=23.8805970149254, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787493, Length=375, Percent_Identity=25.6, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789386, Length=355, Percent_Identity=27.0422535211268, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321181, Length=345, Percent_Identity=25.7971014492754, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.3.1.32 [H]

Molecular weight: Translated: 42006; Mature: 41875

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVRASEMTFTETERVTMGRPAREAIRDQAEKMGARRVFILASDTLRTKTDEIAAIERE
CCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHH
LGERHAATFSGIAPHAPRSDVLRAADAARAAGADLIVSVGGGSVTDSAKIVALLLKHDVR
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
SVEAFEPLHVYVDGDGNVINPLAVGPDVRVICVPTTLSGGEFNALSGATDEAVQHKQGYE
HHHCCCCEEEEECCCCCEECCEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
HRDMAPVMVVLDPALTVYTPEWLWLSTGVRSVDHAVETLASHLSNDFADGLADSALRLLV
CCCCCCEEEEECCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLSRAKADPTDMEARLKCQIGAWQSMISIIGGVPMGASHAIGHILGGTCDVPHGYTSCV
HHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
MSPYVLAWNAEHDASRQGRILQCLGNTHASASEALDAFIRSLGMPRTLSEVGVGEDRFQQ
CCCEEEEECCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
VAEYTMLDIWGRTNPRPVRSPADILEILRKAA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GSVRASEMTFTETERVTMGRPAREAIRDQAEKMGARRVFILASDTLRTKTDEIAAIERE
CCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHH
LGERHAATFSGIAPHAPRSDVLRAADAARAAGADLIVSVGGGSVTDSAKIVALLLKHDVR
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHH
SVEAFEPLHVYVDGDGNVINPLAVGPDVRVICVPTTLSGGEFNALSGATDEAVQHKQGYE
HHHCCCCEEEEECCCCCEECCEECCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCC
HRDMAPVMVVLDPALTVYTPEWLWLSTGVRSVDHAVETLASHLSNDFADGLADSALRLLV
CCCCCCEEEEECCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLSRAKADPTDMEARLKCQIGAWQSMISIIGGVPMGASHAIGHILGGTCDVPHGYTSCV
HHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHH
MSPYVLAWNAEHDASRQGRILQCLGNTHASASEALDAFIRSLGMPRTLSEVGVGEDRFQQ
CCCEEEEECCCCCCCCCCCHHEECCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHH
VAEYTMLDIWGRTNPRPVRSPADILEILRKAA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]