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The map label for this gene is amtB [H]

Identifier: 87200323

GI number: 87200323

Start: 2449396

End: 2450709

Strand: Direct

Name: amtB [H]

Synonym: Saro_2309

Alternate gene names: 87200323

Gene position: 2449396-2450709 (Clockwise)

Preceding gene: 87200322

Following gene: 87200324

Centisome position: 68.77

GC content: 67.35

Gene sequence:

>1314_bases
ATGCGGAAATATCTGGCGACGGCGCTTGCCGCCACCGTGGCGTTGCTTCCGGTTCTGGCACACGCGCAAACAGGCGCTGT
CAGCGTCGAAGACGTGGCCGACACCGGCGACACGGCTTGGATCCTCGTCTCCTCGGCCTTCGTCCTGATGATGGCGATGC
CGGGCCTCACCCTCTTCTACGGCGGCCTCGTCCGCGCCAAGGGCTTCCTTGCCGTCCTCGTCCAGGTCGGCGCGATCGCC
GCCGTGGCTTCGGTCCTCTGGGTCGCGGTCGGATACACGCTTGCCTTCGGTGACGTGTCGGGCGGCTGGATCGGTGGCGG
AAATGCGTGGATGCTGCTGAACCTCGGCAACGTCCGAGCCAACACCGCCATTCCGGAAAGCGCCTTCGCGCTGTTCCAGA
TGTGTTTCGCACTCATCACGCCCGCGCTGATGGTCGGCGCCTGGGTGGACCGCGCACGCTTCGGCTGGGTCGTGGCCTTC
TCCGCCCTGTGGAGCCTTATCGTCTACGCGCCGGTTGCCCACTGGGTCTGGGGCGGCGGCTGGCTTGCCACCCGGTTCGG
CACACTCGATTTCGCCGGCGGCATCGTGGTCCACACCACGGCCGGTGTCTCGGCGCTCGTCGTCGCCATGCTCCTCGGCA
AGCGCGCCGGCTTCCCCCGGACGCTCATGCTGCCCCACAGCCCCTCGCTGACCATGGCGGGCGCCGCGCTCCTGTGGGTG
GGCTGGTTCGGCTTCAACGGTGGCTCCGCACTGGCCGCCAATGACGATGCAGCCTCGGCCATCATCAACACCCACGTCGC
GGCCTGCGTCGCGGCACTTGTATGGCTGATCATCGAAAAGCTCAGCGTGGGCAAGCCGACCTCGGTCGGCTTCGCGACCG
GCGCGGTCGCCGGCCTCGCCACGGTTACCCCCGCCGCCGGGTTCATTTCGCCCGGCGCGGCAATGCTCTTCGGCGCCGCC
GCCGCATGCGTCTGCTATCCGATGATCCAGATCGTGAAGCAGCGCCTCGAGATCGACGATTCGCTCGATGTCTTCGCGGT
CCACGGCGTTGGCGGCATGATCGGTTCGCTTCTTCTGGCGGTCTTCCTCGCGCCCTCCTTCGGCGGCACGGGCTATGCCG
ATGGCATGAACCTCGCGCGCCAGGCCGTCGCCCAGTTTGCCGGTGTCGGCATCGTCGCGGCATGGAGCGCGTTTGCCACC
GTCATCTGCGCCCTCATGGTATCGATGGTCATTCCGATGCGCGTGACCGAGGACGAGGAACGCGAAGGCCTCGACATCAC
CAGCCACGGCGAACGCGCCTGGGAATTCGATTGA

Upstream 100 bases:

>100_bases
CGCACGTTCCCTTGTACACGGCAGCATTGTTGCTCTAGAGCGCCTGCCCCAGAGGATCTGCCGTCCGCCGTCACGTGCGG
ACGCGGGGGGAGTCGTTCAT

Downstream 100 bases:

>100_bases
GCGTCTCCGGGACGGGCCTTGCGCCGGCAAGCCAGCGGCCATAGGCTCGTCCCATGACCTCATCACAAGCGCAGGACCTG
TGCACCGCCTGCGGCATGTG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MRKYLATALAATVALLPVLAHAQTGAVSVEDVADTGDTAWILVSSAFVLMMAMPGLTLFYGGLVRAKGFLAVLVQVGAIA
AVASVLWVAVGYTLAFGDVSGGWIGGGNAWMLLNLGNVRANTAIPESAFALFQMCFALITPALMVGAWVDRARFGWVVAF
SALWSLIVYAPVAHWVWGGGWLATRFGTLDFAGGIVVHTTAGVSALVVAMLLGKRAGFPRTLMLPHSPSLTMAGAALLWV
GWFGFNGGSALAANDDAASAIINTHVAACVAALVWLIIEKLSVGKPTSVGFATGAVAGLATVTPAAGFISPGAAMLFGAA
AACVCYPMIQIVKQRLEIDDSLDVFAVHGVGGMIGSLLLAVFLAPSFGGTGYADGMNLARQAVAQFAGVGIVAAWSAFAT
VICALMVSMVIPMRVTEDEEREGLDITSHGERAWEFD

Sequences:

>Translated_437_residues
MRKYLATALAATVALLPVLAHAQTGAVSVEDVADTGDTAWILVSSAFVLMMAMPGLTLFYGGLVRAKGFLAVLVQVGAIA
AVASVLWVAVGYTLAFGDVSGGWIGGGNAWMLLNLGNVRANTAIPESAFALFQMCFALITPALMVGAWVDRARFGWVVAF
SALWSLIVYAPVAHWVWGGGWLATRFGTLDFAGGIVVHTTAGVSALVVAMLLGKRAGFPRTLMLPHSPSLTMAGAALLWV
GWFGFNGGSALAANDDAASAIINTHVAACVAALVWLIIEKLSVGKPTSVGFATGAVAGLATVTPAAGFISPGAAMLFGAA
AACVCYPMIQIVKQRLEIDDSLDVFAVHGVGGMIGSLLLAVFLAPSFGGTGYADGMNLARQAVAQFAGVGIVAAWSAFAT
VICALMVSMVIPMRVTEDEEREGLDITSHGERAWEFD
>Mature_437_residues
MRKYLATALAATVALLPVLAHAQTGAVSVEDVADTGDTAWILVSSAFVLMMAMPGLTLFYGGLVRAKGFLAVLVQVGAIA
AVASVLWVAVGYTLAFGDVSGGWIGGGNAWMLLNLGNVRANTAIPESAFALFQMCFALITPALMVGAWVDRARFGWVVAF
SALWSLIVYAPVAHWVWGGGWLATRFGTLDFAGGIVVHTTAGVSALVVAMLLGKRAGFPRTLMLPHSPSLTMAGAALLWV
GWFGFNGGSALAANDDAASAIINTHVAACVAALVWLIIEKLSVGKPTSVGFATGAVAGLATVTPAAGFISPGAAMLFGAA
AACVCYPMIQIVKQRLEIDDSLDVFAVHGVGGMIGSLLLAVFLAPSFGGTGYADGMNLARQAVAQFAGVGIVAAWSAFAT
VICALMVSMVIPMRVTEDEEREGLDITSHGERAWEFD

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=425, Percent_Identity=42.1176470588235, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17550182, Length=440, Percent_Identity=29.3181818181818, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550180, Length=435, Percent_Identity=27.1264367816092, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=426, Percent_Identity=25.5868544600939, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32564042, Length=439, Percent_Identity=23.4624145785877, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=37.3522458628842, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6325396, Length=417, Percent_Identity=30.6954436450839, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321559, Length=424, Percent_Identity=30.6603773584906, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=25.8454106280193, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45145; Mature: 45145

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYLATALAATVALLPVLAHAQTGAVSVEDVADTGDTAWILVSSAFVLMMAMPGLTLFY
CHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
GGLVRAKGFLAVLVQVGAIAAVASVLWVAVGYTLAFGDVSGGWIGGGNAWMLLNLGNVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCEEEEEEECCCCC
NTAIPESAFALFQMCFALITPALMVGAWVDRARFGWVVAFSALWSLIVYAPVAHWVWGGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WLATRFGTLDFAGGIVVHTTAGVSALVVAMLLGKRAGFPRTLMLPHSPSLTMAGAALLWV
CHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHH
GWFGFNGGSALAANDDAASAIINTHVAACVAALVWLIIEKLSVGKPTSVGFATGAVAGLA
HHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
TVTPAAGFISPGAAMLFGAAAACVCYPMIQIVKQRLEIDDSLDVFAVHGVGGMIGSLLLA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
VFLAPSFGGTGYADGMNLARQAVAQFAGVGIVAAWSAFATVICALMVSMVIPMRVTEDEE
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REGLDITSHGERAWEFD
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKYLATALAATVALLPVLAHAQTGAVSVEDVADTGDTAWILVSSAFVLMMAMPGLTLFY
CHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
GGLVRAKGFLAVLVQVGAIAAVASVLWVAVGYTLAFGDVSGGWIGGGNAWMLLNLGNVRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCEEEEEEECCCCC
NTAIPESAFALFQMCFALITPALMVGAWVDRARFGWVVAFSALWSLIVYAPVAHWVWGGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WLATRFGTLDFAGGIVVHTTAGVSALVVAMLLGKRAGFPRTLMLPHSPSLTMAGAALLWV
CHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHH
GWFGFNGGSALAANDDAASAIINTHVAACVAALVWLIIEKLSVGKPTSVGFATGAVAGLA
HHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
TVTPAAGFISPGAAMLFGAAAACVCYPMIQIVKQRLEIDDSLDVFAVHGVGGMIGSLLLA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHH
VFLAPSFGGTGYADGMNLARQAVAQFAGVGIVAAWSAFATVICALMVSMVIPMRVTEDEE
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REGLDITSHGERAWEFD
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]