Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 87200310

GI number: 87200310

Start: 2439162

End: 2439830

Strand: Reverse

Name: nuoE [H]

Synonym: Saro_2296

Alternate gene names: 87200310

Gene position: 2439830-2439162 (Counterclockwise)

Preceding gene: 87200311

Following gene: 87200309

Centisome position: 68.5

GC content: 64.87

Gene sequence:

>669_bases
ATGGCTGATCGCTTTATCGAACCCGACAGCCCCGAACTGCGCGCGCGCTGGGGTGGCTTCGAGTGGACGCCCGAGAACGC
CGAGAAGGCGAAGCAGATCGTCGCCCGCTATCCGGCCGGACGCCAGCGTTCGGCGGTGATGCCGCTGCTCGACCTTGCGC
AGCGCCAGGTCGGTGCGGAACTGAACACGCAAGGCTGGCTGCCGATCCCGGTGATGGAATACGTGGCAAGCTACCTCGAC
ATGCCGGTTATCCGCGTGGTCGAGGTGGCGACGTTCTACACGATGTACAACATCGCCCCGATCGGCCGTTTCCATGTGCA
GGTGTGCGGCACCACGCCGTGCATGTTGCGCGGTTCGGACGACATCCTCGCCGCCTGCAAGGGCAAGGGGCTGAAGAAGG
GCCACACCACGCCGGACGGCATGTTCACGCTGACCGAGGTCGAGTGCATGGGCAATTGCGCCTCGGCGCCGATGGTCCAG
ATCAACGACGACAACTACGAAGACCTGACTGCCGCCGACATGGAGCGCATCCTTGACGAGCTGGCTGAGGGCAAGCAGCC
GAAGACCGGCACGCAGCTTCCCGGTCGTCACACTGTCGAGCCGGCGGGGGCGCTCAGCAACCTGACCGCGATGGTGACCG
AGAACCACGACTATCGGGGGGAGTGGTAA

Upstream 100 bases:

>100_bases
GCGAAGGCGACCGACACCTTGCCCATGCTGGTTGCGGCAATCGGCGGGTTCGAGCTGTTCCACTACGCGCAGGACATCTG
GCTGAAGCGGGGGCGGACCA

Downstream 100 bases:

>100_bases
GATGGCCCTTCAGGACAAGGACCGCATCTTCACCAACGTCTACGGCTTCCAGCCGTGGAACCTTGCAGCCGCGCGTGCCC
GCGGGGACTGGGACAACACC

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MADRFIEPDSPELRARWGGFEWTPENAEKAKQIVARYPAGRQRSAVMPLLDLAQRQVGAELNTQGWLPIPVMEYVASYLD
MPVIRVVEVATFYTMYNIAPIGRFHVQVCGTTPCMLRGSDDILAACKGKGLKKGHTTPDGMFTLTEVECMGNCASAPMVQ
INDDNYEDLTAADMERILDELAEGKQPKTGTQLPGRHTVEPAGALSNLTAMVTENHDYRGEW

Sequences:

>Translated_222_residues
MADRFIEPDSPELRARWGGFEWTPENAEKAKQIVARYPAGRQRSAVMPLLDLAQRQVGAELNTQGWLPIPVMEYVASYLD
MPVIRVVEVATFYTMYNIAPIGRFHVQVCGTTPCMLRGSDDILAACKGKGLKKGHTTPDGMFTLTEVECMGNCASAPMVQ
INDDNYEDLTAADMERILDELAEGKQPKTGTQLPGRHTVEPAGALSNLTAMVTENHDYRGEW
>Mature_221_residues
ADRFIEPDSPELRARWGGFEWTPENAEKAKQIVARYPAGRQRSAVMPLLDLAQRQVGAELNTQGWLPIPVMEYVASYLDM
PVIRVVEVATFYTMYNIAPIGRFHVQVCGTTPCMLRGSDDILAACKGKGLKKGHTTPDGMFTLTEVECMGNCASAPMVQI
NDDNYEDLTAADMERILDELAEGKQPKTGTQLPGRHTVEPAGALSNLTAMVTENHDYRGEW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=206, Percent_Identity=55.8252427184466, Blast_Score=224, Evalue=5e-59,
Organism=Escherichia coli, GI1788621, Length=138, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=190, Percent_Identity=51.0526315789474, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI18859877, Length=206, Percent_Identity=50, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI21355353, Length=190, Percent_Identity=42.6315789473684, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24578; Mature: 24447

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRFIEPDSPELRARWGGFEWTPENAEKAKQIVARYPAGRQRSAVMPLLDLAQRQVGAE
CCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
LNTQGWLPIPVMEYVASYLDMPVIRVVEVATFYTMYNIAPIGRFHVQVCGTTPCMLRGSD
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCC
DILAACKGKGLKKGHTTPDGMFTLTEVECMGNCASAPMVQINDDNYEDLTAADMERILDE
HHHHHHCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
LAEGKQPKTGTQLPGRHTVEPAGALSNLTAMVTENHDYRGEW
HHCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCCCCCC
>Mature Secondary Structure 
ADRFIEPDSPELRARWGGFEWTPENAEKAKQIVARYPAGRQRSAVMPLLDLAQRQVGAE
CCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
LNTQGWLPIPVMEYVASYLDMPVIRVVEVATFYTMYNIAPIGRFHVQVCGTTPCMLRGSD
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCC
DILAACKGKGLKKGHTTPDGMFTLTEVECMGNCASAPMVQINDDNYEDLTAADMERILDE
HHHHHHCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHH
LAEGKQPKTGTQLPGRHTVEPAGALSNLTAMVTENHDYRGEW
HHCCCCCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA