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The map label for this gene is nuoF1 [H]

Identifier: 87200309

GI number: 87200309

Start: 2437874

End: 2439160

Strand: Reverse

Name: nuoF1 [H]

Synonym: Saro_2295

Alternate gene names: 87200309

Gene position: 2439160-2437874 (Counterclockwise)

Preceding gene: 87200310

Following gene: 87200308

Centisome position: 68.49

GC content: 66.12

Gene sequence:

>1287_bases
ATGGCCCTTCAGGACAAGGACCGCATCTTCACCAACGTCTACGGCTTCCAGCCGTGGAACCTTGCAGCCGCGCGTGCCCG
CGGGGACTGGGACAACACCAAGGCCCTGATGGAGCGCGGCCAGGACGCGATCATCGAGGAAATCAAGGCCTCGGGCCTGC
GCGGCCGCGGCGGTGCGGGCTTCCCCACCGGGATGAAGTGGTCGTTCATGCCCAAGGAGAGCAAGGACGGTCGTCCTTCA
TTCCTGGTCATCAACGCGGACGAGTCCGAACCCGGTTCGTGCAAGGACCGCGAGATCATTCGCCACGATCCGCACAAGCT
GATCGAAGGCGCGCTGATCGCAGGCTATGCGATGCGCGCCCGCGCCGCCTACATCTACATTCGCGGCGAATACATCCGCG
AGGCCGATACGCTGTTCGCCGCCGTGCAGGAGGCCTATGACGCGGGCCTGATCGGCAAGAACGCGAGCGGGTCGGGCTAC
GATTTCGACGTCTTCGTCCACCGCGGTGCCGGCGCCTACATCTGCGGCGAAGAGACCGCGATGATCGAGAGCCTAGAGGG
CAAGAAGGGCCAGCCCCGCCTCAAGCCGCCGTTCCCGGCCGGCGCGGGCCTTTACGGCTGCCCGACCACCGTCAACAACG
TGGAATCGATCGCGGTCGCGCCGACGATCCTGCGTCGCGGGGCTTCGTGGTTCTCGTCGTTCGGGCGCGAGAACAACAAG
GGCACCAAGCTCTTCCAGATCAGCGGCCACGTGAACCGGCCCTGCGTGGTCGAGGAAGAGATGAGCATCCCGTTCAGCGA
GCTGATCGAGAAGCACTGCGGCGGCATTCGTGGCGGCAAGGACAACCTGCTCGCCGTGATCCCCGGCGGCTCGTCGGTTC
CGCTGGTTCCGGCCGCCGAGATCTGGGACGCGCCGATGGACTTCGACGGGTTGCGCGCTCTCGGTTCCGGCCTCGGCACC
GCCGCCGTCATCGTCATGGACAAGTCGACCGACATCGTCCGCGCGATCAGCCGCCTTTCGTACTTCTACAAGCACGAGAG
CTGCGGCCAGTGCACGCCGTGCCGCGAAGGCACCGGCTGGATGTGGCGCGTGATGGAACGCCTGCGCACGGGCGACGCGG
ACGTCGAGGAAATCGACATGCTGTTCAACGTGACCAAGCAGGTCGAAGGCCACACGATCTGTGCGCTGGGTGACGCGGCC
GCATGGCCGATCCAGGGCCTGATCCGCCATTTCCGCCCTGAGATCGAGCGCCGGATCGCCGAGAACGCTCGGGAGGCAGC
GGAGTGA

Upstream 100 bases:

>100_bases
ACGCAGCTTCCCGGTCGTCACACTGTCGAGCCGGCGGGGGCGCTCAGCAACCTGACCGCGATGGTGACCGAGAACCACGA
CTATCGGGGGGAGTGGTAAG

Downstream 100 bases:

>100_bases
CGACCATCGCCCTTCATCGTCACCCTGAACTTGTTTCAGGGTCCATCTCTCGTCCCGCACCGTCCGTGTTCGTTGCGGGA
TGGATGCTGAAACAAGTTCA

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 1; NDH-1 subunit F 1 [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MALQDKDRIFTNVYGFQPWNLAAARARGDWDNTKALMERGQDAIIEEIKASGLRGRGGAGFPTGMKWSFMPKESKDGRPS
FLVINADESEPGSCKDREIIRHDPHKLIEGALIAGYAMRARAAYIYIRGEYIREADTLFAAVQEAYDAGLIGKNASGSGY
DFDVFVHRGAGAYICGEETAMIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAVAPTILRRGASWFSSFGRENNK
GTKLFQISGHVNRPCVVEEEMSIPFSELIEKHCGGIRGGKDNLLAVIPGGSSVPLVPAAEIWDAPMDFDGLRALGSGLGT
AAVIVMDKSTDIVRAISRLSYFYKHESCGQCTPCREGTGWMWRVMERLRTGDADVEEIDMLFNVTKQVEGHTICALGDAA
AWPIQGLIRHFRPEIERRIAENAREAAE

Sequences:

>Translated_428_residues
MALQDKDRIFTNVYGFQPWNLAAARARGDWDNTKALMERGQDAIIEEIKASGLRGRGGAGFPTGMKWSFMPKESKDGRPS
FLVINADESEPGSCKDREIIRHDPHKLIEGALIAGYAMRARAAYIYIRGEYIREADTLFAAVQEAYDAGLIGKNASGSGY
DFDVFVHRGAGAYICGEETAMIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAVAPTILRRGASWFSSFGRENNK
GTKLFQISGHVNRPCVVEEEMSIPFSELIEKHCGGIRGGKDNLLAVIPGGSSVPLVPAAEIWDAPMDFDGLRALGSGLGT
AAVIVMDKSTDIVRAISRLSYFYKHESCGQCTPCREGTGWMWRVMERLRTGDADVEEIDMLFNVTKQVEGHTICALGDAA
AWPIQGLIRHFRPEIERRIAENAREAAE
>Mature_427_residues
ALQDKDRIFTNVYGFQPWNLAAARARGDWDNTKALMERGQDAIIEEIKASGLRGRGGAGFPTGMKWSFMPKESKDGRPSF
LVINADESEPGSCKDREIIRHDPHKLIEGALIAGYAMRARAAYIYIRGEYIREADTLFAAVQEAYDAGLIGKNASGSGYD
FDVFVHRGAGAYICGEETAMIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAVAPTILRRGASWFSSFGRENNKG
TKLFQISGHVNRPCVVEEEMSIPFSELIEKHCGGIRGGKDNLLAVIPGGSSVPLVPAAEIWDAPMDFDGLRALGSGLGTA
AVIVMDKSTDIVRAISRLSYFYKHESCGQCTPCREGTGWMWRVMERLRTGDADVEEIDMLFNVTKQVEGHTICALGDAAA
WPIQGLIRHFRPEIERRIAENAREAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=418, Percent_Identity=70.0956937799043, Blast_Score=619, Evalue=1e-177,
Organism=Homo sapiens, GI260656005, Length=418, Percent_Identity=70.0956937799043, Blast_Score=618, Evalue=1e-177,
Organism=Escherichia coli, GI1788620, Length=408, Percent_Identity=42.8921568627451, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17531727, Length=423, Percent_Identity=69.0307328605201, Blast_Score=616, Evalue=1e-177,
Organism=Drosophila melanogaster, GI19920794, Length=426, Percent_Identity=69.7183098591549, Blast_Score=634, Evalue=0.0,
Organism=Drosophila melanogaster, GI45550452, Length=423, Percent_Identity=65.7210401891253, Blast_Score=585, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24653819, Length=423, Percent_Identity=47.0449172576832, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24653817, Length=423, Percent_Identity=43.9716312056738, Blast_Score=357, Evalue=9e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46839; Mature: 46708

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQDKDRIFTNVYGFQPWNLAAARARGDWDNTKALMERGQDAIIEEIKASGLRGRGGAG
CCCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
FPTGMKWSFMPKESKDGRPSFLVINADESEPGSCKDREIIRHDPHKLIEGALIAGYAMRA
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHC
RAAYIYIRGEYIREADTLFAAVQEAYDAGLIGKNASGSGYDFDVFVHRGAGAYICGEETA
EEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHH
MIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAVAPTILRRGASWFSSFGRENNK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTKLFQISGHVNRPCVVEEEMSIPFSELIEKHCGGIRGGKDNLLAVIPGGSSVPLVPAAE
CCEEEEECCCCCCCEEECHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHH
IWDAPMDFDGLRALGSGLGTAAVIVMDKSTDIVRAISRLSYFYKHESCGQCTPCREGTGW
HCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
MWRVMERLRTGDADVEEIDMLFNVTKQVEGHTICALGDAAAWPIQGLIRHFRPEIERRIA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ENAREAAE
HHHHHHCC
>Mature Secondary Structure 
ALQDKDRIFTNVYGFQPWNLAAARARGDWDNTKALMERGQDAIIEEIKASGLRGRGGAG
CCCCCHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
FPTGMKWSFMPKESKDGRPSFLVINADESEPGSCKDREIIRHDPHKLIEGALIAGYAMRA
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHC
RAAYIYIRGEYIREADTLFAAVQEAYDAGLIGKNASGSGYDFDVFVHRGAGAYICGEETA
EEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEECCHHH
MIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAVAPTILRRGASWFSSFGRENNK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GTKLFQISGHVNRPCVVEEEMSIPFSELIEKHCGGIRGGKDNLLAVIPGGSSVPLVPAAE
CCEEEEECCCCCCCEEECHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCHH
IWDAPMDFDGLRALGSGLGTAAVIVMDKSTDIVRAISRLSYFYKHESCGQCTPCREGTGW
HCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
MWRVMERLRTGDADVEEIDMLFNVTKQVEGHTICALGDAAAWPIQGLIRHFRPEIERRIA
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
ENAREAAE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]