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The map label for this gene is dnaG [H]

Identifier: 87200248

GI number: 87200248

Start: 2375707

End: 2377572

Strand: Direct

Name: dnaG [H]

Synonym: Saro_2234

Alternate gene names: 87200248

Gene position: 2375707-2377572 (Clockwise)

Preceding gene: 87200247

Following gene: 87200249

Centisome position: 66.7

GC content: 66.83

Gene sequence:

>1866_bases
ATGACCCTCACCCCGCAATGGCTGGACGAACTCCGCACCCGCATCTCGCTGTCGGGGCTGATCGGGCGTTCGGTTCGCGT
CCAGAAGGCGGGGCGGGAGTTCAAGGCCTGTTGCCCGTTCCATAACGAGAAGACGCCCAGCTTCACGATCAACGACGAGA
AAGGTTTCTATCACTGCTTCGGCTGCGGCGCGCATGGGGATGCCATTCGCTGGATGACCGATCACCAGGGCCTCCCGTTC
ATGGAGGCAGTGAAGGAACTGGCGCTGCAGGCAGGCATGGAAGTCCCTGCCCCCGATCCGCGCGCGGCGAAACGGGCGGA
GCAGCAGAAGTCGCTGCACGACGTGATGCAGGCAGCGCAGGATTTCTTCGTGCGCTGCCTTGGCGAGGAGCGCGGGACCG
AGGCGCGGCGCTATCTGGCCTCGCGCGGGTTTCCGGCTGGAATCGTTCGGGATTTCGGCTTCGGCTTCGCACCGGACAGC
CGCACCGCCCTGAAGGAGGCGCTGTCCGGGTTTCCGGACGAAATGCTGATCGAGGCAGGCCTGCGCATCGTCGTGGAAGG
CAAGGAGCCCTACGACCGGTTCCGGGGCCGCCTGATGCTGCCGATCCTAGATCCGCGCGGACGGGTGATAGCGTTCGGCG
GACGCATCCTGACGGCGGAAAAGACCGACGCCCCCAAATACCTCAATTCGCCCGACACGCCGCTCTTCGACAAGGGGCGG
ACGGTCTATAATCTCCACCGCGCCGGCCCCGCCTCGCGCCAGAACGGGCGCGTGGTGGTGGTCGAAGGGTACATGGACGT
GGTCGCACTTGCGGCCGCGGGCATCGGTGAGGCGGTGGCGCCGCTGGGCACCGCGCTGACCGAGCACCAGATCGAGCGGC
TGTGGCGGCTGGTGGAAACACCGACGCTTTGCTTCGACGGCGATGCCGCCGGGCAGCGCGCGGCGATGCGGGCGATTACC
CGCGCCCTTCCCCTGCTCCGCCCCGGCCATTCGCTTCGCATCGTGACTCTGCCGCAGGGAATGGACCCGGACGACGTCGT
TAAGAAGCAGGGTCCGGCGGCGATGGAAGCGCTGCTCGACGGGGCGCGCAGCCTCGTCGACGTGCTGTGGGAAGTGGAGC
GCGATGCCGCGCCGCTCGCGACTCCGGAGGACAAGGCCGGGCTCAAGGCGCGGTTGCTCGCCCATTGCGAGACCATCCAG
CATCCCGACATCAAATCGCTGTACCGTCGCGAGCTGACAGAGCGATTCGGCGAACTTGCCTTTGCCCGCAAGGAGCGCGC
GCCGTTCCAGCCCCGGCAGGGCGGATCGGCGGCCCGTGGCAAGGGACAGCCGTGGAAGCCCGCGCCGCCGCCGCTTGCCG
AGCAGGACAAGGCAAAGATGCTGCGAATCGGGACCGGGCCAAGCACGAGCCTGCTGGCCGCGGTGCTGGACGGACTGCTG
CGCCATCCGCAACAGATTGCCCGCCACGCCGATTCGCTTGCCAGGCTTCGACCGGACGATCCGGCCACCTGCGCGCTGAT
CGACGCCCTGCTGGTCCTCTCGGATGGCGTGCAACCGCTTGAAAGCGAAGCGCTGCGCCCCATATTGCTGGAACGAGGGC
TTCGGCTCCCGTCGGCGCAGGACTACGCCGGAATGCGATTCGGCTTTCTGACCGGTAGCGGTGATTCGGCAGCGGATGAA
CTTGCCGAAGCAGTCGCCATCCTGGTCGAGTGGCCGGCGGTGGAAGAGGCGCTGGCAGAGGCCACGAGACGCGCCGAAAC
CGAGCTTTCCGACGAAAACTTTGCCGAACAGCAAAGATTGCGCGCGCGGAGTATGGCATTGCGTGCCCGTTTGGGGCAAA
TGGGCCGTGCTCGTGCTGCGTTATAA

Upstream 100 bases:

>100_bases
AGGTGATCGGGCAAACGTTGGACAACATTCCCTCCCCCGGCCCCTCCCTCAGGCGGGAGGGGCGTTTGCGGACCCGCGCG
CACCGCGGCGGCGAGACCAC

Downstream 100 bases:

>100_bases
TTATACGAACGCGCAACCCGGGTCCGCCAAGACCCGGCTCTAGATGGTGGTACCGGGGTTAAATGGCTTCGAACGACAAG
ACCGACAGCGGCGATGCTCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTLTPQWLDELRTRISLSGLIGRSVRVQKAGREFKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDHQGLPF
MEAVKELALQAGMEVPAPDPRAAKRAEQQKSLHDVMQAAQDFFVRCLGEERGTEARRYLASRGFPAGIVRDFGFGFAPDS
RTALKEALSGFPDEMLIEAGLRIVVEGKEPYDRFRGRLMLPILDPRGRVIAFGGRILTAEKTDAPKYLNSPDTPLFDKGR
TVYNLHRAGPASRQNGRVVVVEGYMDVVALAAAGIGEAVAPLGTALTEHQIERLWRLVETPTLCFDGDAAGQRAAMRAIT
RALPLLRPGHSLRIVTLPQGMDPDDVVKKQGPAAMEALLDGARSLVDVLWEVERDAAPLATPEDKAGLKARLLAHCETIQ
HPDIKSLYRRELTERFGELAFARKERAPFQPRQGGSAARGKGQPWKPAPPPLAEQDKAKMLRIGTGPSTSLLAAVLDGLL
RHPQQIARHADSLARLRPDDPATCALIDALLVLSDGVQPLESEALRPILLERGLRLPSAQDYAGMRFGFLTGSGDSAADE
LAEAVAILVEWPAVEEALAEATRRAETELSDENFAEQQRLRARSMALRARLGQMGRARAAL

Sequences:

>Translated_621_residues
MTLTPQWLDELRTRISLSGLIGRSVRVQKAGREFKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDHQGLPF
MEAVKELALQAGMEVPAPDPRAAKRAEQQKSLHDVMQAAQDFFVRCLGEERGTEARRYLASRGFPAGIVRDFGFGFAPDS
RTALKEALSGFPDEMLIEAGLRIVVEGKEPYDRFRGRLMLPILDPRGRVIAFGGRILTAEKTDAPKYLNSPDTPLFDKGR
TVYNLHRAGPASRQNGRVVVVEGYMDVVALAAAGIGEAVAPLGTALTEHQIERLWRLVETPTLCFDGDAAGQRAAMRAIT
RALPLLRPGHSLRIVTLPQGMDPDDVVKKQGPAAMEALLDGARSLVDVLWEVERDAAPLATPEDKAGLKARLLAHCETIQ
HPDIKSLYRRELTERFGELAFARKERAPFQPRQGGSAARGKGQPWKPAPPPLAEQDKAKMLRIGTGPSTSLLAAVLDGLL
RHPQQIARHADSLARLRPDDPATCALIDALLVLSDGVQPLESEALRPILLERGLRLPSAQDYAGMRFGFLTGSGDSAADE
LAEAVAILVEWPAVEEALAEATRRAETELSDENFAEQQRLRARSMALRARLGQMGRARAAL
>Mature_620_residues
TLTPQWLDELRTRISLSGLIGRSVRVQKAGREFKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDHQGLPFM
EAVKELALQAGMEVPAPDPRAAKRAEQQKSLHDVMQAAQDFFVRCLGEERGTEARRYLASRGFPAGIVRDFGFGFAPDSR
TALKEALSGFPDEMLIEAGLRIVVEGKEPYDRFRGRLMLPILDPRGRVIAFGGRILTAEKTDAPKYLNSPDTPLFDKGRT
VYNLHRAGPASRQNGRVVVVEGYMDVVALAAAGIGEAVAPLGTALTEHQIERLWRLVETPTLCFDGDAAGQRAAMRAITR
ALPLLRPGHSLRIVTLPQGMDPDDVVKKQGPAAMEALLDGARSLVDVLWEVERDAAPLATPEDKAGLKARLLAHCETIQH
PDIKSLYRRELTERFGELAFARKERAPFQPRQGGSAARGKGQPWKPAPPPLAEQDKAKMLRIGTGPSTSLLAAVLDGLLR
HPQQIARHADSLARLRPDDPATCALIDALLVLSDGVQPLESEALRPILLERGLRLPSAQDYAGMRFGFLTGSGDSAADEL
AEAVAILVEWPAVEEALAEATRRAETELSDENFAEQQRLRARSMALRARLGQMGRARAAL

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=425, Percent_Identity=41.8823529411765, Blast_Score=332, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68002; Mature: 67871

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTPQWLDELRTRISLSGLIGRSVRVQKAGREFKACCPFHNEKTPSFTINDEKGFYHCF
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCCCCCCCCCEEECCCCCEEEEE
GCGAHGDAIRWMTDHQGLPFMEAVKELALQAGMEVPAPDPRAAKRAEQQKSLHDVMQAAQ
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DFFVRCLGEERGTEARRYLASRGFPAGIVRDFGFGFAPDSRTALKEALSGFPDEMLIEAG
HHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
LRIVVEGKEPYDRFRGRLMLPILDPRGRVIAFGGRILTAEKTDAPKYLNSPDTPLFDKGR
CEEEEECCCHHHHHCCEEEEEEECCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC
TVYNLHRAGPASRQNGRVVVVEGYMDVVALAAAGIGEAVAPLGTALTEHQIERLWRLVET
EEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PTLCFDGDAAGQRAAMRAITRALPLLRPGHSLRIVTLPQGMDPDDVVKKQGPAAMEALLD
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHH
GARSLVDVLWEVERDAAPLATPEDKAGLKARLLAHCETIQHPDIKSLYRRELTERFGELA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FARKERAPFQPRQGGSAARGKGQPWKPAPPPLAEQDKAKMLRIGTGPSTSLLAAVLDGLL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RHPQQIARHADSLARLRPDDPATCALIDALLVLSDGVQPLESEALRPILLERGLRLPSAQ
HCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
DYAGMRFGFLTGSGDSAADELAEAVAILVEWPAVEEALAEATRRAETELSDENFAEQQRL
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RARSMALRARLGQMGRARAAL
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTPQWLDELRTRISLSGLIGRSVRVQKAGREFKACCPFHNEKTPSFTINDEKGFYHCF
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHCCCCCCCCCCEEECCCCCEEEEE
GCGAHGDAIRWMTDHQGLPFMEAVKELALQAGMEVPAPDPRAAKRAEQQKSLHDVMQAAQ
ECCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DFFVRCLGEERGTEARRYLASRGFPAGIVRDFGFGFAPDSRTALKEALSGFPDEMLIEAG
HHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHCC
LRIVVEGKEPYDRFRGRLMLPILDPRGRVIAFGGRILTAEKTDAPKYLNSPDTPLFDKGR
CEEEEECCCHHHHHCCEEEEEEECCCCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC
TVYNLHRAGPASRQNGRVVVVEGYMDVVALAAAGIGEAVAPLGTALTEHQIERLWRLVET
EEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PTLCFDGDAAGQRAAMRAITRALPLLRPGHSLRIVTLPQGMDPDDVVKKQGPAAMEALLD
CCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHH
GARSLVDVLWEVERDAAPLATPEDKAGLKARLLAHCETIQHPDIKSLYRRELTERFGELA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FARKERAPFQPRQGGSAARGKGQPWKPAPPPLAEQDKAKMLRIGTGPSTSLLAAVLDGLL
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RHPQQIARHADSLARLRPDDPATCALIDALLVLSDGVQPLESEALRPILLERGLRLPSAQ
HCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
DYAGMRFGFLTGSGDSAADELAEAVAILVEWPAVEEALAEATRRAETELSDENFAEQQRL
CCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RARSMALRARLGQMGRARAAL
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]