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The map label for this gene is carA [H]

Identifier: 87200246

GI number: 87200246

Start: 2373388

End: 2374563

Strand: Reverse

Name: carA [H]

Synonym: Saro_2231

Alternate gene names: 87200246

Gene position: 2374563-2373388 (Counterclockwise)

Preceding gene: 87200251

Following gene: 87200245

Centisome position: 66.67

GC content: 65.48

Gene sequence:

>1176_bases
ATGGCCGACCCCGCACTTTCGCACGCGCCTCAACCAGATGGCGCGACGGGAGTCCTTGTACTTGCCGATGGGTCTGTTGC
CTGGGGACGTGGATTTGGCGCTGCTGGTGCGGCCGTTGGCGAGGTGTGCTTCAACACCGCGATGACCGGATACCAGGAAG
TGATGACCGATCCGTCCTATGCGGGACAGGTCGTCACCTTCACTTTTCCGCACATCGGCAACGTCGGCACCAATCCGGAC
GACATGGAAAGCCATGACACGCCGGGCGCCGTGGGCTGCGTGGTGCGCGAGGACGTGACCGCGCCCGCCAACTTCCGCGC
TGCCGGCACCTTCCAGCAGTGGATGGAGCAGCAGGGCAAGATCGGCATCTCCGGCCTCGACACCCGCGCGCTGACGCGCC
GTATCCGCCTTTCAGGCGCGCCGAACGCGGTGATCGCGCACGATCCGAAGGGCCAGTTCGACATTCCCGCGCTGATCGAA
CGCGCGCGGTCCTGGCCCGGTCTCGAAGGCATGGACCTTGCCCGCGTCGTTACACGCGACGGGCAGGAAGCCTGGGAAGG
CTCGGTCTGGCATCTGGGCGAGGGCTTCACCAAGCCCCAAGGCACTGGCCGTCCGCACGTCGTCGCCATGGATTTCGGCG
CGAAGGACAACATTTTCCGCAATCTGGTGAAGGCCGGCGCGGACGTGACGATCGTGCCCGCCGAAACCAGCCTCGAGACG
ATCCTTTCGCTCAAGCCCGCAGGCGTGTTCCTGTCGAACGGTCCGGGCGACCCGGCGGCGACCGGCGCCTACGCCGTGCC
GGTGATCCAGAAGCTGCTCGAGATGGACATGCCGGTTTTCGGCATCTGCCTCGGCCACCAGATGCTGGCGCTCGCCGCCG
GTGCACGCACCACCAAGATGCACCAGGGCCACCGCGGTGCGAACCACCCGGTCAAGCGCATCGAGGACGGCGTCGTCGAG
ATCACCTCGATGAACCACGGCTTTGCTGTCGACAACGCGCCGGGCGCGCTGGGCGACAAGGTGATCGAAACTCACGTCTC
GCTGTTCGACGGCTCGAACTGCGGTATCGCGGTCAAGGGCAAGAAGGCGTTCGGCGTGCAATACCACCCGGAAGCTTCGC
CCGGCCCGCAGGACAGTTTCTACCTGTTCGAGAAGTTTGTAGCGTCGCTCGTTTGA

Upstream 100 bases:

>100_bases
AACTTTCGGGAATCGCTAGGTTGACGCTGCGGCAATGCAGGTCTAGCAGCCGCCCCTTAGCAACATCGCCAGAACCCCTG
CCAACGGAGCGCCCCATATC

Downstream 100 bases:

>100_bases
GCGGCTGACCCAGAGTATTCCCTCCTCCGGCGCGACCGACACGCGACGGGGGCTGAAACACGAGAGCGATAATGCCCAAA
CGCACTGACATCTCCTCGAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MADPALSHAPQPDGATGVLVLADGSVAWGRGFGAAGAAVGEVCFNTAMTGYQEVMTDPSYAGQVVTFTFPHIGNVGTNPD
DMESHDTPGAVGCVVREDVTAPANFRAAGTFQQWMEQQGKIGISGLDTRALTRRIRLSGAPNAVIAHDPKGQFDIPALIE
RARSWPGLEGMDLARVVTRDGQEAWEGSVWHLGEGFTKPQGTGRPHVVAMDFGAKDNIFRNLVKAGADVTIVPAETSLET
ILSLKPAGVFLSNGPGDPAATGAYAVPVIQKLLEMDMPVFGICLGHQMLALAAGARTTKMHQGHRGANHPVKRIEDGVVE
ITSMNHGFAVDNAPGALGDKVIETHVSLFDGSNCGIAVKGKKAFGVQYHPEASPGPQDSFYLFEKFVASLV

Sequences:

>Translated_391_residues
MADPALSHAPQPDGATGVLVLADGSVAWGRGFGAAGAAVGEVCFNTAMTGYQEVMTDPSYAGQVVTFTFPHIGNVGTNPD
DMESHDTPGAVGCVVREDVTAPANFRAAGTFQQWMEQQGKIGISGLDTRALTRRIRLSGAPNAVIAHDPKGQFDIPALIE
RARSWPGLEGMDLARVVTRDGQEAWEGSVWHLGEGFTKPQGTGRPHVVAMDFGAKDNIFRNLVKAGADVTIVPAETSLET
ILSLKPAGVFLSNGPGDPAATGAYAVPVIQKLLEMDMPVFGICLGHQMLALAAGARTTKMHQGHRGANHPVKRIEDGVVE
ITSMNHGFAVDNAPGALGDKVIETHVSLFDGSNCGIAVKGKKAFGVQYHPEASPGPQDSFYLFEKFVASLV
>Mature_390_residues
ADPALSHAPQPDGATGVLVLADGSVAWGRGFGAAGAAVGEVCFNTAMTGYQEVMTDPSYAGQVVTFTFPHIGNVGTNPDD
MESHDTPGAVGCVVREDVTAPANFRAAGTFQQWMEQQGKIGISGLDTRALTRRIRLSGAPNAVIAHDPKGQFDIPALIER
ARSWPGLEGMDLARVVTRDGQEAWEGSVWHLGEGFTKPQGTGRPHVVAMDFGAKDNIFRNLVKAGADVTIVPAETSLETI
LSLKPAGVFLSNGPGDPAATGAYAVPVIQKLLEMDMPVFGICLGHQMLALAAGARTTKMHQGHRGANHPVKRIEDGVVEI
TSMNHGFAVDNAPGALGDKVIETHVSLFDGSNCGIAVKGKKAFGVQYHPEASPGPQDSFYLFEKFVASLV

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=385, Percent_Identity=37.6623376623377, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI21361331, Length=392, Percent_Identity=35.7142857142857, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI169790915, Length=392, Percent_Identity=35.7142857142857, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=52, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI193204318, Length=391, Percent_Identity=33.7595907928389, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=34.5268542199489, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=32.9113924050633, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=35.2185089974293, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=35.2185089974293, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41148; Mature: 41017

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPALSHAPQPDGATGVLVLADGSVAWGRGFGAAGAAVGEVCFNTAMTGYQEVMTDPSY
CCCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AGQVVTFTFPHIGNVGTNPDDMESHDTPGAVGCVVREDVTAPANFRAAGTFQQWMEQQGK
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCC
IGISGLDTRALTRRIRLSGAPNAVIAHDPKGQFDIPALIERARSWPGLEGMDLARVVTRD
CCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCC
GQEAWEGSVWHLGEGFTKPQGTGRPHVVAMDFGAKDNIFRNLVKAGADVTIVPAETSLET
CHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHHH
ILSLKPAGVFLSNGPGDPAATGAYAVPVIQKLLEMDMPVFGICLGHQMLALAAGARTTKM
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
HQGHRGANHPVKRIEDGVVEITSMNHGFAVDNAPGALGDKVIETHVSLFDGSNCGIAVKG
HCCCCCCCCCHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHEECCCCCEEEEC
KKAFGVQYHPEASPGPQDSFYLFEKFVASLV
CEEECEEECCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
ADPALSHAPQPDGATGVLVLADGSVAWGRGFGAAGAAVGEVCFNTAMTGYQEVMTDPSY
CCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
AGQVVTFTFPHIGNVGTNPDDMESHDTPGAVGCVVREDVTAPANFRAAGTFQQWMEQQGK
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCC
IGISGLDTRALTRRIRLSGAPNAVIAHDPKGQFDIPALIERARSWPGLEGMDLARVVTRD
CCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCC
GQEAWEGSVWHLGEGFTKPQGTGRPHVVAMDFGAKDNIFRNLVKAGADVTIVPAETSLET
CHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHHH
ILSLKPAGVFLSNGPGDPAATGAYAVPVIQKLLEMDMPVFGICLGHQMLALAAGARTTKM
HHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
HQGHRGANHPVKRIEDGVVEITSMNHGFAVDNAPGALGDKVIETHVSLFDGSNCGIAVKG
HCCCCCCCCCHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHEECCCCCEEEEC
KKAFGVQYHPEASPGPQDSFYLFEKFVASLV
CEEECEEECCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA