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The map label for this gene is greA

Identifier: 87200244

GI number: 87200244

Start: 2369385

End: 2369861

Strand: Reverse

Name: greA

Synonym: Saro_2229

Alternate gene names: 87200244

Gene position: 2369861-2369385 (Counterclockwise)

Preceding gene: 87200245

Following gene: 87200238

Centisome position: 66.54

GC content: 59.54

Gene sequence:

>477_bases
ATGGCGAGTGTGGAAAAGCTGCCGATGCTTGCGGAAGGCTACGAGAAGCTGACTGCAGAGCTGAAAGCGCTGCGCGAGGA
GCGCCCCCGGATCGTCGACGCGATCGAGGAAGCGCGCGCCCACGGCGATCTTTCCGAAAATGCGGAATACCATGCCGCCA
AGGAGCGTCAGGGCCAGGTCGAGGCAACCATCGCCGATCTCGAGGATCGGGTAAGCCGTGCCCAGATCATCGATCCGACG
ACTCTGTCGGGCGACAAGATCATCTTCGGCGCGACCGTCACCCTGCTCGATGACGACGAAAAGCCGGTGAAGTACCAGAT
TGTCGGTCCTTACGAGGCCGATGCCAAGAAGGGCATGATCAGCTACAATTCGCCGCTGGGCAAGGCGCTGATCGGCCGCA
AGGTCGATGAAGAAATCGAGGTCACCGTGCCTTCGGGCGACAAGTTCTATCTTGTCCAGAAGATCGAGTTCATCTGA

Upstream 100 bases:

>100_bases
GTGTCCGGCTCGACGCATTCGGCGTCGGTCGGCGGGGCCAGGTTTTCCCTTGGAAGCCGTAACGGCAACCAGGGGCGTGA
GACAGGAAAGAGACTGACGG

Downstream 100 bases:

>100_bases
CGGCCGGAGTGCGGATCCAGCAGGATCGGCATCGATACCGGAGATAAAAAGCCCCGGATCGCTCCGGGGCTTTTTTTGAT
CGACTTGATCAGGCCTTGGG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MASVEKLPMLAEGYEKLTAELKALREERPRIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIADLEDRVSRAQIIDPT
TLSGDKIIFGATVTLLDDDEKPVKYQIVGPYEADAKKGMISYNSPLGKALIGRKVDEEIEVTVPSGDKFYLVQKIEFI

Sequences:

>Translated_158_residues
MASVEKLPMLAEGYEKLTAELKALREERPRIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIADLEDRVSRAQIIDPT
TLSGDKIIFGATVTLLDDDEKPVKYQIVGPYEADAKKGMISYNSPLGKALIGRKVDEEIEVTVPSGDKFYLVQKIEFI
>Mature_157_residues
ASVEKLPMLAEGYEKLTAELKALREERPRIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIADLEDRVSRAQIIDPTT
LSGDKIIFGATVTLLDDDEKPVKYQIVGPYEADAKKGMISYNSPLGKALIGRKVDEEIEVTVPSGDKFYLVQKIEFI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=46.2025316455696, Blast_Score=135, Evalue=9e-34,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=34.8387096774194, Blast_Score=91, Evalue=3e-20,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_NOVAD (Q2G656)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497501.1
- ProteinModelPortal:   Q2G656
- SMR:   Q2G656
- STRING:   Q2G656
- GeneID:   3916545
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2229
- NMPDR:   fig|48935.1.peg.1218
- eggNOG:   COG0782
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- PhylomeDB:   Q2G656
- ProtClustDB:   PRK00226
- BioCyc:   NARO279238:SARO_2229-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17534; Mature: 17403

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVEKLPMLAEGYEKLTAELKALREERPRIVDAIEEARAHGDLSENAEYHAAKERQGQV
CCCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCH
EATIADLEDRVSRAQIIDPTTLSGDKIIFGATVTLLDDDEKPVKYQIVGPYEADAKKGMI
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEE
SYNSPLGKALIGRKVDEEIEVTVPSGDKFYLVQKIEFI
ECCCCHHHHHHCCCCCCCEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
ASVEKLPMLAEGYEKLTAELKALREERPRIVDAIEEARAHGDLSENAEYHAAKERQGQV
CCCHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCH
EATIADLEDRVSRAQIIDPTTLSGDKIIFGATVTLLDDDEKPVKYQIVGPYEADAKKGMI
HHHHHHHHHHHHHHEECCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEE
SYNSPLGKALIGRKVDEEIEVTVPSGDKFYLVQKIEFI
ECCCCHHHHHHCCCCCCCEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA