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The map label for this gene is eno

Identifier: 87200238

GI number: 87200238

Start: 2365698

End: 2366984

Strand: Reverse

Name: eno

Synonym: Saro_2223

Alternate gene names: 87200238

Gene position: 2366984-2365698 (Counterclockwise)

Preceding gene: 87200244

Following gene: 87200235

Centisome position: 66.46

GC content: 65.19

Gene sequence:

>1287_bases
ATGACCGCGATCATTGACATCCATGCACGCGAAATCCTCGACAGCCGCGGCAACCCGACCGTGGAAGTCGACGTGCTGCT
GGAAGACGGCAGCTTCGGCCGCGCCGCCGTTCCTTCGGGCGCGTCCACCGGCGCGCATGAAGCGGTCGAACTGCGCGACG
GCGACAAGGCACGCTACCTCGGCAAGGGCGTGACCAAGGCCGTCACCGCCGTGAACTCGGACATCGCCGAATGCATTCTT
GGCCTGGACGCTGAAGACCAGCGCGACATCGATCTTGCCATGATCGAACTGGACGGCACCGAGAACAAGAGCCGCCTTGG
CGCGAACGCCATCCTCGGCACCAGCCTTGCCGTGGCGAAGGCCGCCGCCGATGCGCGGGGCCTGCCGCTCTACAGCTACG
TCGGCGGCGTTTCCGCGCATGTTCTGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAGCATGCCGACAATCCGATC
GACTTCCAGGAATTCATGATCATGCCGGTCGGCGCGCCCTCGCTCGCCGAAGCCGTGCGCTGGGGCGCGGAAGTGTTCCA
CACCCTGAAGAAGGGCCTGCACGAAAAGGGTCTTGCCACTGCCGTGGGCGACGAGGGCGGCTTCGCTCCCAACCTTGCCA
GCACGCGCGACGCGCTCGACTTCGTGATGGCTTCGATCGAGAAGGCCGGTTTCAAGCCGGGCGAGGACATGATGCTCGCG
CTCGACTGCGCGGCGACCGAATTCTTCAAGAACGGCAAGTACGAGATCAGCGGTGAAGGCCTCTCGCTCTCGCCCGACGC
CATGGCCGACTACCTTGCCGCCCTGTGCGATGCCTATCCGATCATCTCGATCGAGGATGGCATGGGCGAGGACGATTTCG
AGGGCTGGGCCGCGCTGACGGCCAAGGTCGGCAAGCGCGTGCAGCTCGTCGGTGACGACCTTTTCGTGACGAACCCGAAG
CGCCTCGAGATGGGCATTGGCAAGGGGCTGGCGAACTCGCTGCTGGTCAAGGTCAACCAGATCGGGTCGCTCACCGAGAC
GCTCGAAGCTGTCAGCATCGCACAGCGCAACGGCTACACCGCCGTCATGTCGCACCGTTCGGGCGAGACCGAGGACGCGA
CCATCGCCGACCTCGCGGTTGCCACCAACTGCGGCCAGATCAAGACCGGTTCGCTCGCCCGTTCGGACCGTCTCGCCAAG
TACAACCAGCTCATCCGCATCGAGGAAGAGCTGGGCGTCTCGGCGCGCTATGCCGGCAAGACCGCGTTCGGTCGCCTCGG
CGCCTGA

Upstream 100 bases:

>100_bases
TGTTGCACTGCCAACGCGGCTTGCCTGTAGGGGTTCCAGCCGATAGGAGCCGCGCCAAACCTTGGCGTCTTTCCTATCGC
CACACTGCAGGGAGCAGACC

Downstream 100 bases:

>100_bases
CCGCGCTTCATTGCGAAACACGAAAAGGGCGGCCTCACGGGGCCGCCCTTTTTGCGTTTCGGACCTCGGGGCTCACGTGA
TCCGGGAGGATCGCGGTCGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKARYLGKGVTKAVTAVNSDIAECIL
GLDAEDQRDIDLAMIELDGTENKSRLGANAILGTSLAVAKAAADARGLPLYSYVGGVSAHVLPVPMMNIINGGEHADNPI
DFQEFMIMPVGAPSLAEAVRWGAEVFHTLKKGLHEKGLATAVGDEGGFAPNLASTRDALDFVMASIEKAGFKPGEDMMLA
LDCAATEFFKNGKYEISGEGLSLSPDAMADYLAALCDAYPIISIEDGMGEDDFEGWAALTAKVGKRVQLVGDDLFVTNPK
RLEMGIGKGLANSLLVKVNQIGSLTETLEAVSIAQRNGYTAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGVSARYAGKTAFGRLGA

Sequences:

>Translated_428_residues
MTAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKARYLGKGVTKAVTAVNSDIAECIL
GLDAEDQRDIDLAMIELDGTENKSRLGANAILGTSLAVAKAAADARGLPLYSYVGGVSAHVLPVPMMNIINGGEHADNPI
DFQEFMIMPVGAPSLAEAVRWGAEVFHTLKKGLHEKGLATAVGDEGGFAPNLASTRDALDFVMASIEKAGFKPGEDMMLA
LDCAATEFFKNGKYEISGEGLSLSPDAMADYLAALCDAYPIISIEDGMGEDDFEGWAALTAKVGKRVQLVGDDLFVTNPK
RLEMGIGKGLANSLLVKVNQIGSLTETLEAVSIAQRNGYTAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGVSARYAGKTAFGRLGA
>Mature_427_residues
TAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKARYLGKGVTKAVTAVNSDIAECILG
LDAEDQRDIDLAMIELDGTENKSRLGANAILGTSLAVAKAAADARGLPLYSYVGGVSAHVLPVPMMNIINGGEHADNPID
FQEFMIMPVGAPSLAEAVRWGAEVFHTLKKGLHEKGLATAVGDEGGFAPNLASTRDALDFVMASIEKAGFKPGEDMMLAL
DCAATEFFKNGKYEISGEGLSLSPDAMADYLAALCDAYPIISIEDGMGEDDFEGWAALTAKVGKRVQLVGDDLFVTNPKR
LEMGIGKGLANSLLVKVNQIGSLTETLEAVSIAQRNGYTAVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEELGVSARYAGKTAFGRLGA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=53.5962877030162, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=54.7785547785548, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=52.3041474654378, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=52.3041474654378, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=47.4537037037037, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=61.8823529411765, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=55.2447552447552, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=55.2447552447552, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=49.7354497354497, Blast_Score=188, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.6944444444444, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.6944444444444, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.6944444444444, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=49.5391705069124, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=49.0783410138249, Blast_Score=373, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=52.1028037383178, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=52.1028037383178, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=52.1028037383178, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=52.1028037383178, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=52.1028037383178, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=52.1028037383178, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_NOVAD (Q2G662)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497495.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2G662
- SMR:   Q2G662
- STRING:   Q2G662
- GeneID:   3916539
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2223
- NMPDR:   fig|48935.1.peg.1212
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q2G662
- ProtClustDB:   PRK00077
- BioCyc:   NARO279238:SARO_2223-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45065; Mature: 44933

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKARYL
CCEEEEEHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVTKAVTAVNSDIAECILGLDAEDQRDIDLAMIELDGTENKSRLGANAILGTSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAADARGLPLYSYVGGVSAHVLPVPMMNIINGGEHADNPIDFQEFMIMPVGAPSLAEAVR
HHHCCCCCCHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
WGAEVFHTLKKGLHEKGLATAVGDEGGFAPNLASTRDALDFVMASIEKAGFKPGEDMMLA
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFFKNGKYEISGEGLSLSPDAMADYLAALCDAYPIISIEDGMGEDDFEGWAALT
EHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
AKVGKRVQLVGDDLFVTNPKRLEMGIGKGLANSLLVKVNQIGSLTETLEAVSIAQRNGYT
HHHCCEEEEECCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCE
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGVSARYAGK
EEEECCCCCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHHHEEEHHHCCCEEEECCC
TAFGRLGA
CCCCCCCC
>Mature Secondary Structure 
TAIIDIHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAVELRDGDKARYL
CEEEEEHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVTKAVTAVNSDIAECILGLDAEDQRDIDLAMIELDGTENKSRLGANAILGTSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHHHHH
AAADARGLPLYSYVGGVSAHVLPVPMMNIINGGEHADNPIDFQEFMIMPVGAPSLAEAVR
HHHCCCCCCHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHH
WGAEVFHTLKKGLHEKGLATAVGDEGGFAPNLASTRDALDFVMASIEKAGFKPGEDMMLA
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEE
LDCAATEFFKNGKYEISGEGLSLSPDAMADYLAALCDAYPIISIEDGMGEDDFEGWAALT
EHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
AKVGKRVQLVGDDLFVTNPKRLEMGIGKGLANSLLVKVNQIGSLTETLEAVSIAQRNGYT
HHHCCEEEEECCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCE
AVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGVSARYAGK
EEEECCCCCCCCCCHHHHHEECCCCCCCCCCCHHHHHHHHHHHHEEEHHHCCCEEEECCC
TAFGRLGA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA