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The map label for this gene is traI [H]

Identifier: 87200199

GI number: 87200199

Start: 2324303

End: 2325931

Strand: Reverse

Name: traI [H]

Synonym: Saro_2183

Alternate gene names: 87200199

Gene position: 2325931-2324303 (Counterclockwise)

Preceding gene: 87200200

Following gene: 87200198

Centisome position: 65.31

GC content: 65.44

Gene sequence:

>1629_bases
ATGCTGTCGGTAGCCAATGTCCGCACCGCCGGCGGGGCGGCCAACTACTTCGCTGCCGACAATTACTACACGCGGGCTGA
TGCCGATCGCTCGGGTGAATGGCTGGGCAACGGCGCTGCCGCGCTCGGGCTCAAGGGGGCCGTCGACGCGCGGCAATTCG
AGGCGGTCCTTAAGGGACTGCTGCCCGATGGTAGCCGCGTCGGCAGTGACAACCGGGCGCACCGGGCGGGGACCGATCTC
ACCTTTTCGATGCCGAAAAGCTGGTCGGTCCTTGCTCTGGTGGGCGGCGACAAGAGGATCCTCGATGCCTACGCCGCGGC
TGTCCGGGAAACCCTGCGCTGGGCCGAGAAGAACCTTGCCGAGACCCGGATGGAGGTTCGGGGCAGAGAGCGTGCGGTCC
AGACCGGCAACCTTGCGATCGCGCTCTTCCAGCACGACACCAACCGCAACCAGGAGCCCAACGCCCACTTCCATGCCGTC
ATTGCCAATGTGACGAGAGGGGCCGATGGCAAATGGCGGGCGCTGCGCAATGACAAGCTCTGGCAGCACAATACTTTGCT
CAATGCCATGACGATGGCGCGGTTTCGTCTGAGCGTCGAGAAGCTCGGCTACGAGGTCGGGGAGTTCGGCAAGCACGGCA
ATTTCGAGGCCGTCGGTGTGCCCAAAGACGTGCGCGATGCCTTCAGTTCGCGGCGGGCGGAGATTCTCGACCGCCTGGCA
ACGATGGAAGGAAGGGGGCTCGCTGCTCGCAACGCAGCAAACCTCATGACCCGGGCAGACAAGGGGCGGATCGAGGATCG
CGATGCGCTGCGGCAGCAGTGGCGGGATACGTCCACAAGGCTGGGCTTTGACCCCGCCATGGTCATTGCCCGGTCCAATG
CTCGCAGCGCGCAGGACCTTGGCAATTTGACTGGCGTTGGGTCCACGATGCGCGCGATAGTCCGCAATGCTCAGGCCCTT
GCAACCTCCTTGGCCGAGCGCCTCGGCCTGCGGGAAGGCGACCCGCTGATACCGGCCCGGCTGACCAATCGCAGTGTCGA
GCAGGTCGCTGCAATTCATGCAGTCGCCTCGGCTGTGCGGCATCTGGGCGAGCGGGAGGCGGCCTTCACCCGGGCCGAAA
TCTACCGTGCAGCGCTTGGCTTTGCGCTCCCAACCTCGCTCGCTGACATCGAGCAGCGCGTCGACCAGCTGCTCCGCCAG
GGACATCTGGAGAAGGGCAGGGGTGCCGACCGGGAACTGCTGACGACACGCGATGCCATCAGTCTCGAACAGCGCATCAT
CGCCGGGGTTGAAGACGGGCGCGGCACCGCGCCGGCGATTGTTTCTCCCGACATTGCCGGGTCCCGGCTGCAGGCCCTGT
CCCAGATCAAATACGGGCTCACCTTGAACGCTGGACAGGAAGGGGCAGGGCGCCTGCTGCTTGGCTCGAACAACCGGATC
GTCGCGATCCAGGGCGTCGCGGGCGCAGGCAAGAGCACGGTCCTCAAACCTGTCGCCGATATCCTGCGCGAGGAGGGCAG
GAGTGTGCTCGGGCTGTCGAAGCCGCCGCAGCCTGGTTGTCGCTTTCGACAGCCGAGCGCGAGGCAACCGCGATTTATGC
CTCAGGCCGAAACTTGCGCAGCGCCGTGA

Upstream 100 bases:

>100_bases
GGTTTGCGGGAGCACGAGCCTCCGCCGATCACTGGTCCCGATCTTGGTGACATGGAGATGTGAGCATGGCGCTGACTGCC
TCGGGATGGCGGAGCGATCG

Downstream 100 bases:

>100_bases
ACGAGGCTGTGCAGACAGGCCTCAAATCCAGCGGCGAGCTTGGTCCTGACACCATGCGGCTCCAAACCCTGTCGCGCGTC
AATGTAACCCGCGAGGAGCT

Product: TrwC protein

Products: NA

Alternate protein names: DNA helicase I [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLSVANVRTAGGAANYFAADNYYTRADADRSGEWLGNGAAALGLKGAVDARQFEAVLKGLLPDGSRVGSDNRAHRAGTDL
TFSMPKSWSVLALVGGDKRILDAYAAAVRETLRWAEKNLAETRMEVRGRERAVQTGNLAIALFQHDTNRNQEPNAHFHAV
IANVTRGADGKWRALRNDKLWQHNTLLNAMTMARFRLSVEKLGYEVGEFGKHGNFEAVGVPKDVRDAFSSRRAEILDRLA
TMEGRGLAARNAANLMTRADKGRIEDRDALRQQWRDTSTRLGFDPAMVIARSNARSAQDLGNLTGVGSTMRAIVRNAQAL
ATSLAERLGLREGDPLIPARLTNRSVEQVAAIHAVASAVRHLGEREAAFTRAEIYRAALGFALPTSLADIEQRVDQLLRQ
GHLEKGRGADRELLTTRDAISLEQRIIAGVEDGRGTAPAIVSPDIAGSRLQALSQIKYGLTLNAGQEGAGRLLLGSNNRI
VAIQGVAGAGKSTVLKPVADILREEGRSVLGLSKPPQPGCRFRQPSARQPRFMPQAETCAAP

Sequences:

>Translated_542_residues
MLSVANVRTAGGAANYFAADNYYTRADADRSGEWLGNGAAALGLKGAVDARQFEAVLKGLLPDGSRVGSDNRAHRAGTDL
TFSMPKSWSVLALVGGDKRILDAYAAAVRETLRWAEKNLAETRMEVRGRERAVQTGNLAIALFQHDTNRNQEPNAHFHAV
IANVTRGADGKWRALRNDKLWQHNTLLNAMTMARFRLSVEKLGYEVGEFGKHGNFEAVGVPKDVRDAFSSRRAEILDRLA
TMEGRGLAARNAANLMTRADKGRIEDRDALRQQWRDTSTRLGFDPAMVIARSNARSAQDLGNLTGVGSTMRAIVRNAQAL
ATSLAERLGLREGDPLIPARLTNRSVEQVAAIHAVASAVRHLGEREAAFTRAEIYRAALGFALPTSLADIEQRVDQLLRQ
GHLEKGRGADRELLTTRDAISLEQRIIAGVEDGRGTAPAIVSPDIAGSRLQALSQIKYGLTLNAGQEGAGRLLLGSNNRI
VAIQGVAGAGKSTVLKPVADILREEGRSVLGLSKPPQPGCRFRQPSARQPRFMPQAETCAAP
>Mature_542_residues
MLSVANVRTAGGAANYFAADNYYTRADADRSGEWLGNGAAALGLKGAVDARQFEAVLKGLLPDGSRVGSDNRAHRAGTDL
TFSMPKSWSVLALVGGDKRILDAYAAAVRETLRWAEKNLAETRMEVRGRERAVQTGNLAIALFQHDTNRNQEPNAHFHAV
IANVTRGADGKWRALRNDKLWQHNTLLNAMTMARFRLSVEKLGYEVGEFGKHGNFEAVGVPKDVRDAFSSRRAEILDRLA
TMEGRGLAARNAANLMTRADKGRIEDRDALRQQWRDTSTRLGFDPAMVIARSNARSAQDLGNLTGVGSTMRAIVRNAQAL
ATSLAERLGLREGDPLIPARLTNRSVEQVAAIHAVASAVRHLGEREAAFTRAEIYRAALGFALPTSLADIEQRVDQLLRQ
GHLEKGRGADRELLTTRDAISLEQRIIAGVEDGRGTAPAIVSPDIAGSRLQALSQIKYGLTLNAGQEGAGRLLLGSNNRI
VAIQGVAGAGKSTVLKPVADILREEGRSVLGLSKPPQPGCRFRQPSARQPRFMPQAETCAAP

Specific function: TraI has been identified as DNA helicase I and it also has an additional activity of site-specific nicking at oriT. DNA helicase I is a potent DNA-dependent ATPase [H]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traI of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014059
- InterPro:   IPR014129
- InterPro:   IPR009767
- InterPro:   IPR014862 [H]

Pfam domain/function: PF07057 TraI; PF08751 TrwC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 58621; Mature: 58621

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVANVRTAGGAANYFAADNYYTRADADRSGEWLGNGAAALGLKGAVDARQFEAVLKGL
CCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
LPDGSRVGSDNRAHRAGTDLTFSMPKSWSVLALVGGDKRILDAYAAAVRETLRWAEKNLA
CCCCCCCCCCCCCHHCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ETRMEVRGRERAVQTGNLAIALFQHDTNRNQEPNAHFHAVIANVTRGADGKWRALRNDKL
HHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCC
WQHNTLLNAMTMARFRLSVEKLGYEVGEFGKHGNFEAVGVPKDVRDAFSSRRAEILDRLA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
TMEGRGLAARNAANLMTRADKGRIEDRDALRQQWRDTSTRLGFDPAMVIARSNARSAQDL
HCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
GNLTGVGSTMRAIVRNAQALATSLAERLGLREGDPLIPARLTNRSVEQVAAIHAVASAVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
HLGEREAAFTRAEIYRAALGFALPTSLADIEQRVDQLLRQGHLEKGRGADRELLTTRDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLEQRIIAGVEDGRGTAPAIVSPDIAGSRLQALSQIKYGLTLNAGQEGAGRLLLGSNNRI
HHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCE
VAIQGVAGAGKSTVLKPVADILREEGRSVLGLSKPPQPGCRFRQPSARQPRFMPQAETCA
EEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
AP
CC
>Mature Secondary Structure
MLSVANVRTAGGAANYFAADNYYTRADADRSGEWLGNGAAALGLKGAVDARQFEAVLKGL
CCCCCCCCCCCCCCCEEECCCCEECCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
LPDGSRVGSDNRAHRAGTDLTFSMPKSWSVLALVGGDKRILDAYAAAVRETLRWAEKNLA
CCCCCCCCCCCCCHHCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
ETRMEVRGRERAVQTGNLAIALFQHDTNRNQEPNAHFHAVIANVTRGADGKWRALRNDKL
HHHHHHHHHHHHEECCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCC
WQHNTLLNAMTMARFRLSVEKLGYEVGEFGKHGNFEAVGVPKDVRDAFSSRRAEILDRLA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
TMEGRGLAARNAANLMTRADKGRIEDRDALRQQWRDTSTRLGFDPAMVIARSNARSAQDL
HCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHH
GNLTGVGSTMRAIVRNAQALATSLAERLGLREGDPLIPARLTNRSVEQVAAIHAVASAVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
HLGEREAAFTRAEIYRAALGFALPTSLADIEQRVDQLLRQGHLEKGRGADRELLTTRDAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
SLEQRIIAGVEDGRGTAPAIVSPDIAGSRLQALSQIKYGLTLNAGQEGAGRLLLGSNNRI
HHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCEEECCCCCCCCEEEECCCCCE
VAIQGVAGAGKSTVLKPVADILREEGRSVLGLSKPPQPGCRFRQPSARQPRFMPQAETCA
EEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2163400; 7915817; 2680768; 2164585; 8736534 [H]