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The map label for this gene is cnrH [H]

Identifier: 87200153

GI number: 87200153

Start: 2277362

End: 2277895

Strand: Reverse

Name: cnrH [H]

Synonym: Saro_2137

Alternate gene names: 87200153

Gene position: 2277895-2277362 (Counterclockwise)

Preceding gene: 87200154

Following gene: 87200152

Centisome position: 63.96

GC content: 62.36

Gene sequence:

>534_bases
TTGGCCGCACTGGCGCTCGCTGGTCGGCAAGACGCCTATCGCGAACTGCTGGCGCGCCACCGTGAATCTGTATTTCGTCT
GGTACGGGCATCCGTCGGCGATCCGCACGAGGCACTCGACATTACCCAGGAGACCTTCATCGCCGCGTTCTCGGCGCTTG
GACGCTACGATCATGAGCGACCGTTCCTGGCCTGGCTTAAGCACATCGCGCTCAACAAGTGCCGGGACTGGGCAAGAAGG
AGAAAGGTGCGTGCCTTCTTCACGCGCGCAATGCCGCTCGAGATGGCTTTCGATGTTTCGGACGATGCCATTTCCGCCGA
CGTACAAGCGGTGGACCGGGCCGAGCTGGCGCGCGTGACCGCGGCCATATCGCGCTTGCCATCCCGTCTGCGCGATGCCT
TGGTCCTCCGCACGATCGATGGGCTCGGCCAGGCTGAAGCCGCAGAGGTGCTGGGTGTCAGCGAAAAAACGGTTGAGACC
CGGCTCTACCGGGCGAGGGGAAAATTAAAAGCGATGCTTGGCGACCAGATCTGA

Upstream 100 bases:

>100_bases
TGAGGCCTGATCAGGCGGTCAAATTCGACAGGACTGTGACCAGAGCCCTGACCCCAGAAGTACGGTGAGCCTTTCGCTGT
CAGAGGGGAGCGACGGCGAA

Downstream 100 bases:

>100_bases
GGGGTAAGGAAAGCGGCTGCGTAGAGAACTATGAAGCCGTCAATCGTACCGGACACCATTCATGTATTCTTTTGCGCTTA
ATCGCCGTACCCTCATTCGT

Product: FecI-like sigma-24 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MAALALAGRQDAYRELLARHRESVFRLVRASVGDPHEALDITQETFIAAFSALGRYDHERPFLAWLKHIALNKCRDWARR
RKVRAFFTRAMPLEMAFDVSDDAISADVQAVDRAELARVTAAISRLPSRLRDALVLRTIDGLGQAEAAEVLGVSEKTVET
RLYRARGKLKAMLGDQI

Sequences:

>Translated_177_residues
MAALALAGRQDAYRELLARHRESVFRLVRASVGDPHEALDITQETFIAAFSALGRYDHERPFLAWLKHIALNKCRDWARR
RKVRAFFTRAMPLEMAFDVSDDAISADVQAVDRAELARVTAAISRLPSRLRDALVLRTIDGLGQAEAAEVLGVSEKTVET
RLYRARGKLKAMLGDQI
>Mature_176_residues
AALALAGRQDAYRELLARHRESVFRLVRASVGDPHEALDITQETFIAAFSALGRYDHERPFLAWLKHIALNKCRDWARRR
KVRAFFTRAMPLEMAFDVSDDAISADVQAVDRAELARVTAAISRLPSRLRDALVLRTIDGLGQAEAAEVLGVSEKTVETR
LYRARGKLKAMLGDQI

Specific function: CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in a RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigm

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=163, Percent_Identity=30.6748466257669, Blast_Score=60, Evalue=6e-11,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 19859; Mature: 19728

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALALAGRQDAYRELLARHRESVFRLVRASVGDPHEALDITQETFIAAFSALGRYDHER
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PFLAWLKHIALNKCRDWARRRKVRAFFTRAMPLEMAFDVSDDAISADVQAVDRAELARVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AAISRLPSRLRDALVLRTIDGLGQAEAAEVLGVSEKTVETRLYRARGKLKAMLGDQI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AALALAGRQDAYRELLARHRESVFRLVRASVGDPHEALDITQETFIAAFSALGRYDHER
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
PFLAWLKHIALNKCRDWARRRKVRAFFTRAMPLEMAFDVSDDAISADVQAVDRAELARVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AAISRLPSRLRDALVLRTIDGLGQAEAAEVLGVSEKTVETRLYRARGKLKAMLGDQI
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380802; 8052622; 10671463; 10671464 [H]