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The map label for this gene is glyS [H]

Identifier: 87200127

GI number: 87200127

Start: 2248829

End: 2251045

Strand: Reverse

Name: glyS [H]

Synonym: Saro_2111

Alternate gene names: 87200127

Gene position: 2251045-2248829 (Counterclockwise)

Preceding gene: 87200128

Following gene: 87200126

Centisome position: 63.2

GC content: 64.73

Gene sequence:

>2217_bases
ATGACCACCGCAGACTTCCTTCTCGAACTGCGCAGCGAGGAAATCCCGGCCCGCATGCAGGCGGGCGCCCGCGCCGATCT
GGAAAAGCTGTTCCGCAAGGAGCTTGACGCCGCCGGCCTGAAGGCGGGTGAGGTGACCGTGTGGTCCACGCCGCGCCGCC
TCGCGCTGATCGCGCGCGCTCTGCCGCTGGCGACGGAAGCCGTGTCCGAGGAAGTAAAGGGGCCACGCACCTCCGCGCCC
GAACAGGCACTCGAAGGCTTCCTGCGCAAGACCGGTCTGACCAGGGACCAGTTGACCGAGCGCGATGGCGTATGGTTTGC
CGTGACGGAGAAGCCGGGACGCGCAACCAGGGATGTGCTCGCGGAAGCAGTCCCCGCGATCATCCGCGCGTTCCCCTGGC
CCAAGAGCCAGCGCTGGGGCGCGGCTTCGCTCTCGACCGAGTCCTTGCGCTGGGTGCGTCCGCTCTCGGGCATCGTAGCG
ATCCTGGGCGATGATCTGGTCGAGTGCGAGATCGGGGCTGTGAAGTCCGGCTATGCCACGCGCGGCCACCGCTTCCATTG
CCCGGGCGAAATCACCATCGGCAGCGCGCACGACTATGCCGACAAGCTCCGCGCCTGCCACGTGATCGTCGATCACGTCG
AGCGCGAGGCGATGGTGCGCGACAAGGCCAAGGCTGCGGCAGAGGCCGCTGGCCTCGTGCTGGTCGAGGACGAAGGTCTG
GTGATCGAGAACGCGGGCCTCACCGAATGGCCGGTCCCGCTGCTCGGCCGCTTCGACGAGGCGTTCCTCGACGTGCCGCC
CGAAGTCATCCAGCTCACCGCCCGCGTCAACCAGAAGTACTTCATCTGCCGTGACAGCGCCGGAAAGCTCGCCAACGCCT
TCGTCTGCACCGCGAACATCGAGGCCAAGGACGGCGGCGCCGCCATCGTCGACGGCAACCGCAAGGTGCTGGCGGCACGC
CTCTCCGACGCCCGCTTCTTCTGGGAGCAGGACAAGAAGAAGCCGCTCGCCGAACAGGCGCAGAAGCTCTCTCGCATCAC
CTTCCACGAAAAGCTCGGCACCGTCGCGGACAAGGTTCAGCGCGTCGTCAAGCTGGCCGAGTGGCTGGCAAGCGAAGGCA
TCGTGCCGAACTGCGATCCCGCTCTGGCGCGGCAGGCTGCGGAACTGTGCAAGGCCGATCTCGTCACCGAAATGGTCGGC
GAATTCCCCGAACTCCAGGGCCTGATGGGCGGCTACTACGCCCGCGCCGAAGGTCTGCCCGATGCCGTGGCAGATGCCAT
CCGCGATCACTACAAGCCGGTCGGGCAGGGCGATGACGTGCCGACCGCGCCGGTAACGGTGGCAGTGGCGCTGGCGGATA
AACTGGATACGTTGGTCGGGTTCTTCCTAATTGATCAGCGCCCCACAGGATCGCGAGACCCGTTTGCGCTTCGACGTGCA
GCTCTTGGCGTGCTCGAACTTCTCAACTGGGGTTCACTGCGCGTCTCGATGTGGGAGGCAATTGCGAAGGCACTCGCCGC
ATTTACAAAGCAGCTTGTTTCTGACGACATGGCGGAAGCTTCCTATGAAGCTTTTCAAACAAACCAGAAGGCAGTGTTTA
CGACACTTCCTGACTTCTTCGCCGACCGCCTCAAAGTCCAGCAACGCGAAGCAGGTGTCCGCCACGACCTGATCGATGCC
GTGTTCGCGCTTGGAGGCGAGGACGATCTCGTCCGCCTGCTTGCCCGCGTCCATGCGCTTCAGGCCTTCGTCGGCACCGA
GGACGGCACCAACCTGCTCGCAGGCTACAAGCGCGCCGCCAACATCCTCAAGAAGGAAGGCTTCGCCGCCAACCCCAACG
CCGGGTCCGAAGGCGAGGGCGTGGTTCCGCCGACGGGCGAGGAAGATCCGCTGGTGCTGGTCGACGATCCGGCGATGGAA
GGCGTCGTCGCGCAGTTCTCGCACCCCGAATGCAACTACCAGCGCGAACCTGCCGAGCAGGCGCTCGTCGATGCGCTGGA
CTACGCAGAACCAGCGGCACGTGGTGCGGTCGAAAGTGAAGATTTCGATTTCGCCATGCGGGTGCTCGCCTCGCTTCGCG
CGCCCATCGACTCCTTCTTCGATCAGGTCACGGTCAATGATGCGGATGCTAACAAGCGTTCCAGTCGCCTTGCCCTGCTC
GACCGGTTCCGTGCTGCGGTGCACAAAGTTGCGGACTTTTCGCGCATAGAAGGCTAA

Upstream 100 bases:

>100_bases
CCATCGGCGCGGCCTCGCACATGCGCAAAGCTTCGGCTCTGGCCGCGCGATGGACCCTGAAACAAGTTCAGGGTGACGAG
ACTCTTGACGGAAACGCAGC

Downstream 100 bases:

>100_bases
AGGGTCGGCCCGACTCCGGGCCGTCCGCCCGGCAAGAACGAGAGAGTTTCAGGGAGTTCGTCTCGGCATGACTGCATACG
TATTCCACTTCGGCGGCGGC

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 738; Mature: 737

Protein sequence:

>738_residues
MTTADFLLELRSEEIPARMQAGARADLEKLFRKELDAAGLKAGEVTVWSTPRRLALIARALPLATEAVSEEVKGPRTSAP
EQALEGFLRKTGLTRDQLTERDGVWFAVTEKPGRATRDVLAEAVPAIIRAFPWPKSQRWGAASLSTESLRWVRPLSGIVA
ILGDDLVECEIGAVKSGYATRGHRFHCPGEITIGSAHDYADKLRACHVIVDHVEREAMVRDKAKAAAEAAGLVLVEDEGL
VIENAGLTEWPVPLLGRFDEAFLDVPPEVIQLTARVNQKYFICRDSAGKLANAFVCTANIEAKDGGAAIVDGNRKVLAAR
LSDARFFWEQDKKKPLAEQAQKLSRITFHEKLGTVADKVQRVVKLAEWLASEGIVPNCDPALARQAAELCKADLVTEMVG
EFPELQGLMGGYYARAEGLPDAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTLVGFFLIDQRPTGSRDPFALRRA
ALGVLELLNWGSLRVSMWEAIAKALAAFTKQLVSDDMAEASYEAFQTNQKAVFTTLPDFFADRLKVQQREAGVRHDLIDA
VFALGGEDDLVRLLARVHALQAFVGTEDGTNLLAGYKRAANILKKEGFAANPNAGSEGEGVVPPTGEEDPLVLVDDPAME
GVVAQFSHPECNYQREPAEQALVDALDYAEPAARGAVESEDFDFAMRVLASLRAPIDSFFDQVTVNDADANKRSSRLALL
DRFRAAVHKVADFSRIEG

Sequences:

>Translated_738_residues
MTTADFLLELRSEEIPARMQAGARADLEKLFRKELDAAGLKAGEVTVWSTPRRLALIARALPLATEAVSEEVKGPRTSAP
EQALEGFLRKTGLTRDQLTERDGVWFAVTEKPGRATRDVLAEAVPAIIRAFPWPKSQRWGAASLSTESLRWVRPLSGIVA
ILGDDLVECEIGAVKSGYATRGHRFHCPGEITIGSAHDYADKLRACHVIVDHVEREAMVRDKAKAAAEAAGLVLVEDEGL
VIENAGLTEWPVPLLGRFDEAFLDVPPEVIQLTARVNQKYFICRDSAGKLANAFVCTANIEAKDGGAAIVDGNRKVLAAR
LSDARFFWEQDKKKPLAEQAQKLSRITFHEKLGTVADKVQRVVKLAEWLASEGIVPNCDPALARQAAELCKADLVTEMVG
EFPELQGLMGGYYARAEGLPDAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTLVGFFLIDQRPTGSRDPFALRRA
ALGVLELLNWGSLRVSMWEAIAKALAAFTKQLVSDDMAEASYEAFQTNQKAVFTTLPDFFADRLKVQQREAGVRHDLIDA
VFALGGEDDLVRLLARVHALQAFVGTEDGTNLLAGYKRAANILKKEGFAANPNAGSEGEGVVPPTGEEDPLVLVDDPAME
GVVAQFSHPECNYQREPAEQALVDALDYAEPAARGAVESEDFDFAMRVLASLRAPIDSFFDQVTVNDADANKRSSRLALL
DRFRAAVHKVADFSRIEG
>Mature_737_residues
TTADFLLELRSEEIPARMQAGARADLEKLFRKELDAAGLKAGEVTVWSTPRRLALIARALPLATEAVSEEVKGPRTSAPE
QALEGFLRKTGLTRDQLTERDGVWFAVTEKPGRATRDVLAEAVPAIIRAFPWPKSQRWGAASLSTESLRWVRPLSGIVAI
LGDDLVECEIGAVKSGYATRGHRFHCPGEITIGSAHDYADKLRACHVIVDHVEREAMVRDKAKAAAEAAGLVLVEDEGLV
IENAGLTEWPVPLLGRFDEAFLDVPPEVIQLTARVNQKYFICRDSAGKLANAFVCTANIEAKDGGAAIVDGNRKVLAARL
SDARFFWEQDKKKPLAEQAQKLSRITFHEKLGTVADKVQRVVKLAEWLASEGIVPNCDPALARQAAELCKADLVTEMVGE
FPELQGLMGGYYARAEGLPDAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTLVGFFLIDQRPTGSRDPFALRRAA
LGVLELLNWGSLRVSMWEAIAKALAAFTKQLVSDDMAEASYEAFQTNQKAVFTTLPDFFADRLKVQQREAGVRHDLIDAV
FALGGEDDLVRLLARVHALQAFVGTEDGTNLLAGYKRAANILKKEGFAANPNAGSEGEGVVPPTGEEDPLVLVDDPAMEG
VVAQFSHPECNYQREPAEQALVDALDYAEPAARGAVESEDFDFAMRVLASLRAPIDSFFDQVTVNDADANKRSSRLALLD
RFRAAVHKVADFSRIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=751, Percent_Identity=34.7536617842876, Blast_Score=343, Evalue=3e-95,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80296; Mature: 80165

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTADFLLELRSEEIPARMQAGARADLEKLFRKELDAAGLKAGEVTVWSTPRRLALIARA
CCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHH
LPLATEAVSEEVKGPRTSAPEQALEGFLRKTGLTRDQLTERDGVWFAVTEKPGRATRDVL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCHHHHHH
AEAVPAIIRAFPWPKSQRWGAASLSTESLRWVRPLSGIVAILGDDLVECEIGAVKSGYAT
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCC
RGHRFHCPGEITIGSAHDYADKLRACHVIVDHVEREAMVRDKAKAAAEAAGLVLVEDEGL
CCCEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCE
VIENAGLTEWPVPLLGRFDEAFLDVPPEVIQLTARVNQKYFICRDSAGKLANAFVCTANI
EEECCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEC
EAKDGGAAIVDGNRKVLAARLSDARFFWEQDKKKPLAEQAQKLSRITFHEKLGTVADKVQ
EECCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVKLAEWLASEGIVPNCDPALARQAAELCKADLVTEMVGEFPELQGLMGGYYARAEGLP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
DAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTLVGFFLIDQRPTGSRDPFALRRA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHH
ALGVLELLNWGSLRVSMWEAIAKALAAFTKQLVSDDMAEASYEAFQTNQKAVFTTLPDFF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVGTEDGTNLLAGYKRAA
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NILKKEGFAANPNAGSEGEGVVPPTGEEDPLVLVDDPAMEGVVAQFSHPECNYQREPAEQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCHHHH
ALVDALDYAEPAARGAVESEDFDFAMRVLASLRAPIDSFFDQVTVNDADANKRSSRLALL
HHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHH
DRFRAAVHKVADFSRIEG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTADFLLELRSEEIPARMQAGARADLEKLFRKELDAAGLKAGEVTVWSTPRRLALIARA
CHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHH
LPLATEAVSEEVKGPRTSAPEQALEGFLRKTGLTRDQLTERDGVWFAVTEKPGRATRDVL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCHHHHHH
AEAVPAIIRAFPWPKSQRWGAASLSTESLRWVRPLSGIVAILGDDLVECEIGAVKSGYAT
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCC
RGHRFHCPGEITIGSAHDYADKLRACHVIVDHVEREAMVRDKAKAAAEAAGLVLVEDEGL
CCCEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCE
VIENAGLTEWPVPLLGRFDEAFLDVPPEVIQLTARVNQKYFICRDSAGKLANAFVCTANI
EEECCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEC
EAKDGGAAIVDGNRKVLAARLSDARFFWEQDKKKPLAEQAQKLSRITFHEKLGTVADKVQ
EECCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RVVKLAEWLASEGIVPNCDPALARQAAELCKADLVTEMVGEFPELQGLMGGYYARAEGLP
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
DAVADAIRDHYKPVGQGDDVPTAPVTVAVALADKLDTLVGFFLIDQRPTGSRDPFALRRA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHH
ALGVLELLNWGSLRVSMWEAIAKALAAFTKQLVSDDMAEASYEAFQTNQKAVFTTLPDFF
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
ADRLKVQQREAGVRHDLIDAVFALGGEDDLVRLLARVHALQAFVGTEDGTNLLAGYKRAA
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NILKKEGFAANPNAGSEGEGVVPPTGEEDPLVLVDDPAMEGVVAQFSHPECNYQREPAEQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCHHHH
ALVDALDYAEPAARGAVESEDFDFAMRVLASLRAPIDSFFDQVTVNDADANKRSSRLALL
HHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHH
DRFRAAVHKVADFSRIEG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA