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The map label for this gene is ppdK [H]

Identifier: 87200126

GI number: 87200126

Start: 2246098

End: 2248761

Strand: Reverse

Name: ppdK [H]

Synonym: Saro_2110

Alternate gene names: 87200126

Gene position: 2248761-2246098 (Counterclockwise)

Preceding gene: 87200127

Following gene: 87200124

Centisome position: 63.14

GC content: 66.89

Gene sequence:

>2664_bases
ATGACTGCATACGTATTCCACTTCGGCGGCGGCGCCACCTCTTCGGAACCGCGCGCGAAGGACAAGACCATCGTCGGCGG
CAAGGGTGCGAACCTGGCCGAGATGGCGGGCATCGGTCTGCCGGTGCCCCCCGGATTTACGATCAGCACCGAAGTCTGCA
CGGCCTACAATGCCAGCGGCAAGACTTTCGACGATGCCCTGCGCGCCGGCGTTGCGGCGGGCGTCTCCCATATCGAGAAG
GCGACAGGCCGCACGTTTGGCGATGCCGCGAATCCTCTGCTGGTCTCGGTCCGCTCGGGCGCGCGCGTCTCGATGCCGGG
CATGATGGACACCGTGCTCAACCTCGGCCTCAACGACGCGACCGTCGAAGGCCTGGCCGCCGGTTCGGGTGATCCGCGCT
TCGCCTGGGACAGCTACCGCCGCTTCATCCAGATGTATTCCGACGTGGTGCTTGGCCTCGACCATCACCGGTTCGAGGAC
GCGCTGGAAATCGTCAAGGAAGACAATGGCTTCTTCAACGACGTGGAACTTGGCGCCAATCATTGGCAGGCGCTTGTCGG
CGAATACAAGTCCATCGTCGAAGACCAGCTCGGCCGCCCGTTCCCGCAGGACGTCCACGAACAGCTCTGGGGCGCGATCG
GCGCCGTGTTCGACAGCTGGGAAAGCGACCGCGCCAAGGTCTATCGCAAGCTCAACGACATTCCGCACGACTGGGGCACC
GCCGTCAACGTGCAGGCCATGGTCTTCGGCAACATGGGCGATACCTCGGCCACCGGCGTCGCGTTCACCCGTGACCCTGC
GACCGGCGAGAAGGCCTACTACGGCGAATGGCTGGTTAACGCACAGGGCGAGGACGTCGTCGCCGGCATCCGCACCCCGC
AGTACCTGACGCTGGCCGCGCGCGAACGTGCCGGGGCCAAGCCGCTGTCGATGGAAGAGGCCATGCCCGGGGCATACGCC
GAACTCGCCGCCGTGTTCGAGCTGCTGGAAAAGCACTATCGCGACATGCAGGACATCGAGTTCACCGTCGAGCAGGGCAA
GCTGTGGATGCTGCAGACCCGTTCGGGCAAGCGCACCGCCAAGGCCGCGCTCAAGATGGCGGTGGACATGGTCGGCGAAG
GCCTGATCGACGAGGCGACCGCGATCAAGCGCATCGACCCGATGGCGCTCGACCAGCTCCTTCACCCCACGCTCGATCCC
AAGGCCGCGCGCGACGTGCTGACCAAGGGCCTGCCGGCTTCGCCCGGCGCGGCTTCGGGCACGGTCGTGTTCGATGCCGA
CACCGCCCAGGCCAAGGCCGAGCGCGGCGATGCGGTGATCCTGGTGCGCGTGGAAACCTCGCCCGAAGACATCCACGGCA
TGCACGCCGCCAAGGGCATCCTCACGGCACGCGGCGGCATGACCAGCCACGCGGCGGTGGTGGCGCGCGGCATGGGCCGT
CCCTGCGTCTCGGGCGCTTCGGCGCTGTCGATCGACATGGCCAGCCGCACCGCGCGCATCGGCAACCGCGAAATCCGCGA
GGGCGACGTGATCACGCTCGACGGTTCGAACGGCGATGTCATGGCCGGCAAGGTCCCGACGGTCGAACCTGAACTGGTCG
GCGATTTCGGCACGCTGATGGCGTGGGCCGACAAGCATCGTCGCATGAAGGTGCGCACCAACGCCGAGACCCCGCTCGAC
TGCCAGGTCGCGCGCCAGTTCGGAGCCGAGGGCATCGGCCTGTGCCGGACCGAGCACATGTTCTTCGAAGCGGGCCGCAT
CAGCGCCGTGCGCCAGATGATCCTTGCCGAGGACGAGGCCGGCCGTCGCGCCGCGCTGGCCAAGCTGCTGCCCGAGCAGC
GCAGCGACTTCCAGTCGATCTTCGAGGTCATGGCCGGCCTGCCCGTCACGATCCGTCTCCTCGACCCGCCGCTGCACGAG
TTCCTGCCCCACGGCGAGACCGAGTTCGCGGAACTGGCCGAAGCGACCGGCCTCGGCATCGAGCATCTGAAACGCCGCGC
GGGCGAACTGCACGAGTTCAATCCGATGCTCGGCCATCGCGGCTGCCGCCTCGGCATCACGTTCCCGGAAATCTACGAGA
TGCAGGCCCGCGCCATCTTCGAGGCAGCCTGCGACGTTGCCGAACAGAGCGGCGATGCGCCGATTCCCGAAGTGATGATC
CCGCTCGTCGCGACGAAGAAGGAACTCGAGATCCTTCGCGCGCTGGTCGACAGGGTGGCGGCAGAGGTCTTCGCCGAGAA
GGGCCGCACGCTCGAATATATGGTCGGCACCATGATCGAACTGCCGCGCGCAGCACTCATGGCGGGCGAGATCGCGGAAG
AGGCGAAGTTCTTCTCGTTCGGCACCAACGACCTCACCCAGACGACGCTGGGCGTCAGCCGTGACGATGCCGCGCGCTTC
CTCAACCCCTATGTCGACCAGGGCATCTACCCGCGCGATCCCTTCGTGAGCCTCGACATCGAGGGCGTCGGCCAGCTTGT
CGAACTTGCGGCGGAGCGCGGGCGCAAGACCCGTCCCGATATCAAGCTCGGCATCTGCGGCGAGCATGGCGGCGATCCGG
CCAGCATCGCGTTCTGTGAGAAGACCGGCCTCGATTACGTCTCGGCCAGCCCCTATCGCGTGCCGATCGCCCGGCTTTCG
GCGGCGCAAGCGGCACTCGCCTGA

Upstream 100 bases:

>100_bases
AAAGTTGCGGACTTTTCGCGCATAGAAGGCTAAAGGGTCGGCCCGACTCCGGGCCGTCCGCCCGGCAAGAACGAGAGAGT
TTCAGGGAGTTCGTCTCGGC

Downstream 100 bases:

>100_bases
TCAGGAGCCGCCTTCGGGCGGCTCAGAACTTCGGCGGGCGGAGCGGTTTCGCCCACCCGCTGAGGGTCAGGATCTCGAAG
CGTTCGGTCACCTTGCCGTC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 887; Mature: 886

Protein sequence:

>887_residues
MTAYVFHFGGGATSSEPRAKDKTIVGGKGANLAEMAGIGLPVPPGFTISTEVCTAYNASGKTFDDALRAGVAAGVSHIEK
ATGRTFGDAANPLLVSVRSGARVSMPGMMDTVLNLGLNDATVEGLAAGSGDPRFAWDSYRRFIQMYSDVVLGLDHHRFED
ALEIVKEDNGFFNDVELGANHWQALVGEYKSIVEDQLGRPFPQDVHEQLWGAIGAVFDSWESDRAKVYRKLNDIPHDWGT
AVNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGEWLVNAQGEDVVAGIRTPQYLTLAARERAGAKPLSMEEAMPGAYA
ELAAVFELLEKHYRDMQDIEFTVEQGKLWMLQTRSGKRTAKAALKMAVDMVGEGLIDEATAIKRIDPMALDQLLHPTLDP
KAARDVLTKGLPASPGAASGTVVFDADTAQAKAERGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGR
PCVSGASALSIDMASRTARIGNREIREGDVITLDGSNGDVMAGKVPTVEPELVGDFGTLMAWADKHRRMKVRTNAETPLD
CQVARQFGAEGIGLCRTEHMFFEAGRISAVRQMILAEDEAGRRAALAKLLPEQRSDFQSIFEVMAGLPVTIRLLDPPLHE
FLPHGETEFAELAEATGLGIEHLKRRAGELHEFNPMLGHRGCRLGITFPEIYEMQARAIFEAACDVAEQSGDAPIPEVMI
PLVATKKELEILRALVDRVAAEVFAEKGRTLEYMVGTMIELPRAALMAGEIAEEAKFFSFGTNDLTQTTLGVSRDDAARF
LNPYVDQGIYPRDPFVSLDIEGVGQLVELAAERGRKTRPDIKLGICGEHGGDPASIAFCEKTGLDYVSASPYRVPIARLS
AAQAALA

Sequences:

>Translated_887_residues
MTAYVFHFGGGATSSEPRAKDKTIVGGKGANLAEMAGIGLPVPPGFTISTEVCTAYNASGKTFDDALRAGVAAGVSHIEK
ATGRTFGDAANPLLVSVRSGARVSMPGMMDTVLNLGLNDATVEGLAAGSGDPRFAWDSYRRFIQMYSDVVLGLDHHRFED
ALEIVKEDNGFFNDVELGANHWQALVGEYKSIVEDQLGRPFPQDVHEQLWGAIGAVFDSWESDRAKVYRKLNDIPHDWGT
AVNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGEWLVNAQGEDVVAGIRTPQYLTLAARERAGAKPLSMEEAMPGAYA
ELAAVFELLEKHYRDMQDIEFTVEQGKLWMLQTRSGKRTAKAALKMAVDMVGEGLIDEATAIKRIDPMALDQLLHPTLDP
KAARDVLTKGLPASPGAASGTVVFDADTAQAKAERGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGR
PCVSGASALSIDMASRTARIGNREIREGDVITLDGSNGDVMAGKVPTVEPELVGDFGTLMAWADKHRRMKVRTNAETPLD
CQVARQFGAEGIGLCRTEHMFFEAGRISAVRQMILAEDEAGRRAALAKLLPEQRSDFQSIFEVMAGLPVTIRLLDPPLHE
FLPHGETEFAELAEATGLGIEHLKRRAGELHEFNPMLGHRGCRLGITFPEIYEMQARAIFEAACDVAEQSGDAPIPEVMI
PLVATKKELEILRALVDRVAAEVFAEKGRTLEYMVGTMIELPRAALMAGEIAEEAKFFSFGTNDLTQTTLGVSRDDAARF
LNPYVDQGIYPRDPFVSLDIEGVGQLVELAAERGRKTRPDIKLGICGEHGGDPASIAFCEKTGLDYVSASPYRVPIARLS
AAQAALA
>Mature_886_residues
TAYVFHFGGGATSSEPRAKDKTIVGGKGANLAEMAGIGLPVPPGFTISTEVCTAYNASGKTFDDALRAGVAAGVSHIEKA
TGRTFGDAANPLLVSVRSGARVSMPGMMDTVLNLGLNDATVEGLAAGSGDPRFAWDSYRRFIQMYSDVVLGLDHHRFEDA
LEIVKEDNGFFNDVELGANHWQALVGEYKSIVEDQLGRPFPQDVHEQLWGAIGAVFDSWESDRAKVYRKLNDIPHDWGTA
VNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGEWLVNAQGEDVVAGIRTPQYLTLAARERAGAKPLSMEEAMPGAYAE
LAAVFELLEKHYRDMQDIEFTVEQGKLWMLQTRSGKRTAKAALKMAVDMVGEGLIDEATAIKRIDPMALDQLLHPTLDPK
AARDVLTKGLPASPGAASGTVVFDADTAQAKAERGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGRP
CVSGASALSIDMASRTARIGNREIREGDVITLDGSNGDVMAGKVPTVEPELVGDFGTLMAWADKHRRMKVRTNAETPLDC
QVARQFGAEGIGLCRTEHMFFEAGRISAVRQMILAEDEAGRRAALAKLLPEQRSDFQSIFEVMAGLPVTIRLLDPPLHEF
LPHGETEFAELAEATGLGIEHLKRRAGELHEFNPMLGHRGCRLGITFPEIYEMQARAIFEAACDVAEQSGDAPIPEVMIP
LVATKKELEILRALVDRVAAEVFAEKGRTLEYMVGTMIELPRAALMAGEIAEEAKFFSFGTNDLTQTTLGVSRDDAARFL
NPYVDQGIYPRDPFVSLDIEGVGQLVELAAERGRKTRPDIKLGICGEHGGDPASIAFCEKTGLDYVSASPYRVPIARLSA
AQAALA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=455, Percent_Identity=26.1538461538462, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789193, Length=429, Percent_Identity=25.6410256410256, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788726, Length=448, Percent_Identity=24.7767857142857, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787994, Length=721, Percent_Identity=25.9361997226075, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI48994992, Length=455, Percent_Identity=23.2967032967033, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 95604; Mature: 95473

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYVFHFGGGATSSEPRAKDKTIVGGKGANLAEMAGIGLPVPPGFTISTEVCTAYNASG
CEEEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCEEEHHHHHEECCCC
KTFDDALRAGVAAGVSHIEKATGRTFGDAANPLLVSVRSGARVSMPGMMDTVLNLGLNDA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHH
TVEGLAAGSGDPRFAWDSYRRFIQMYSDVVLGLDHHRFEDALEIVKEDNGFFNDVELGAN
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCHH
HWQALVGEYKSIVEDQLGRPFPQDVHEQLWGAIGAVFDSWESDRAKVYRKLNDIPHDWGT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
AVNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGEWLVNAQGEDVVAGIRTPQYLTLAA
EEEEEEEEEECCCCCCCCCEEEECCCCCCCCCEECCEEECCCCCCEEEECCCCCEEEEEH
RERAGAKPLSMEEAMPGAYAELAAVFELLEKHYRDMQDIEFTVEQGKLWMLQTRSGKRTA
HHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHEEEEECCCEEEEEECCCCHHH
KAALKMAVDMVGEGLIDEATAIKRIDPMALDQLLHPTLDPKAARDVLTKGLPASPGAASG
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
TVVFDADTAQAKAERGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGR
EEEEECCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCCHHHHHHHHCCCC
PCVSGASALSIDMASRTARIGNREIREGDVITLDGSNGDVMAGKVPTVEPELVGDFGTLM
CCCCCCCCEEEEHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHH
AWADKHRRMKVRTNAETPLDCQVARQFGAEGIGLCRTEHMFFEAGRISAVRQMILAEDEA
HHHHHHCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRRAALAKLLPEQRSDFQSIFEVMAGLPVTIRLLDPPLHEFLPHGETEFAELAEATGLGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCH
EHLKRRAGELHEFNPMLGHRGCRLGITFPEIYEMQARAIFEAACDVAEQSGDAPIPEVMI
HHHHHHHCCHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
PLVATKKELEILRALVDRVAAEVFAEKGRTLEYMVGTMIELPRAALMAGEIAEEAKFFSF
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTNDLTQTTLGVSRDDAARFLNPYVDQGIYPRDPFVSLDIEGVGQLVELAAERGRKTRPD
CCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
IKLGICGEHGGDPASIAFCEKTGLDYVSASPYRVPIARLSAAQAALA
EEEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TAYVFHFGGGATSSEPRAKDKTIVGGKGANLAEMAGIGLPVPPGFTISTEVCTAYNASG
EEEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCEEEHHHHHEECCCC
KTFDDALRAGVAAGVSHIEKATGRTFGDAANPLLVSVRSGARVSMPGMMDTVLNLGLNDA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHH
TVEGLAAGSGDPRFAWDSYRRFIQMYSDVVLGLDHHRFEDALEIVKEDNGFFNDVELGAN
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCHH
HWQALVGEYKSIVEDQLGRPFPQDVHEQLWGAIGAVFDSWESDRAKVYRKLNDIPHDWGT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCC
AVNVQAMVFGNMGDTSATGVAFTRDPATGEKAYYGEWLVNAQGEDVVAGIRTPQYLTLAA
EEEEEEEEEECCCCCCCCCEEEECCCCCCCCCEECCEEECCCCCCEEEECCCCCEEEEEH
RERAGAKPLSMEEAMPGAYAELAAVFELLEKHYRDMQDIEFTVEQGKLWMLQTRSGKRTA
HHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCHHHHEEEEECCCEEEEEECCCCHHH
KAALKMAVDMVGEGLIDEATAIKRIDPMALDQLLHPTLDPKAARDVLTKGLPASPGAASG
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCC
TVVFDADTAQAKAERGDAVILVRVETSPEDIHGMHAAKGILTARGGMTSHAAVVARGMGR
EEEEECCCHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCHHEECCCCCHHHHHHHHCCCC
PCVSGASALSIDMASRTARIGNREIREGDVITLDGSNGDVMAGKVPTVEPELVGDFGTLM
CCCCCCCCEEEEHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHH
AWADKHRRMKVRTNAETPLDCQVARQFGAEGIGLCRTEHMFFEAGRISAVRQMILAEDEA
HHHHHHCEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRRAALAKLLPEQRSDFQSIFEVMAGLPVTIRLLDPPLHEFLPHGETEFAELAEATGLGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCH
EHLKRRAGELHEFNPMLGHRGCRLGITFPEIYEMQARAIFEAACDVAEQSGDAPIPEVMI
HHHHHHHCCHHHCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
PLVATKKELEILRALVDRVAAEVFAEKGRTLEYMVGTMIELPRAALMAGEIAEEAKFFSF
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTNDLTQTTLGVSRDDAARFLNPYVDQGIYPRDPFVSLDIEGVGQLVELAAERGRKTRPD
CCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCC
IKLGICGEHGGDPASIAFCEKTGLDYVSASPYRVPIARLSAAQAALA
EEEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]