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The map label for this gene is cysK [H]

Identifier: 87200119

GI number: 87200119

Start: 2239574

End: 2240491

Strand: Direct

Name: cysK [H]

Synonym: Saro_2103

Alternate gene names: 87200119

Gene position: 2239574-2240491 (Clockwise)

Preceding gene: 87200118

Following gene: 87200120

Centisome position: 62.88

GC content: 65.9

Gene sequence:

>918_bases
ATGAGAGCGGAATCCATCCTCGCCACGATCGGCAACACCCCGCACATTCGCCTGTCCCGCCTGTTTCCCGACCACGAGGT
ATGGGTGAAGTGCGAGCGCGCCAATCCGGGCGGCTCCATCAAGGACAGGATCGGCCTCGCCATGATCGAGGCCGCAGAGG
CCGACGGCAGCCTGAAGCCGGGCGGCACCATCGTCGAGCCCACTTCCGGCAATACCGGCATCGGTCTTGCCATGGCGGCG
GCGGTAAAGGGCTACAAGCTCATCCTCGTCATGCCTGAATCGATGTCGCTTGAACGCCGCCGCCTGATGCTGGCCTATGG
CGCGACCTTCGACCTGACCCCGCGCGAAAAGGGCATGAAGGGCGCGATCGAGCGCGCGAAGGAAATCGTCGATCAGACCG
ACGGCGCATGGATGCCGCAGCAGTTCGACAATCCGGCCAACGTATCGGTTCACGTCCGCACGACCGCCCAGGAAATCCTC
AAGGACTTCGGCAGCGAACCGATCGACGTGCTCATCACCGGCGTCGGCACCGGCGGTCACCTGACCGGTTGCGCCGAGGA
ACTGAAGAAGCACTGGCCCTCGCTCAAGGCCTATGCCGTGGAACCCACCCTTTCCCCGGTCATCTCGGGCGGCCAACCCG
GCCCGCACCCGATCCAGGGCATCGGCGCGGGCTTCATCCCCGGCAACCTCCACACCCAGTCGATCGACGGCGCAATCCAG
GTCGACCCGGCGGACGCCAAGGAAATGGCGCGCCTTTGCGCATCGAAGGAAGGCATGCTGGTCGGCATCTCGTCCGGCGC
AACGCTGGCGGCCATCGCGCAGAAGCTGCCGAGCCTGCCCGCCGGCAGCCGCGTGCTGGGCTTCAACTACGACACCGGCG
AGCGCTATCTCTCGGTGCCGGACTTCCTGCCGGAGTAA

Upstream 100 bases:

>100_bases
CATGGTTTTTCCTTTTCCCGGAACTGAAGAACGCGCGAACCTTTGCAGCGCGATACAGATCGGCCTAAACTCTCCAAACT
CACCACAAGGAGCAAGCGAC

Downstream 100 bases:

>100_bases
CGGACATGGCGGTCGACCAGGTCGACTATGCCGATGGCGAGGTGTCGCTTGCCGGTTTCCTCGCGAAACCGGCGGGCAAG
CCCCGCGCGGCGGTGCTGGT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRAESILATIGNTPHIRLSRLFPDHEVWVKCERANPGGSIKDRIGLAMIEAAEADGSLKPGGTIVEPTSGNTGIGLAMAA
AVKGYKLILVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAKEIVDQTDGAWMPQQFDNPANVSVHVRTTAQEIL
KDFGSEPIDVLITGVGTGGHLTGCAEELKKHWPSLKAYAVEPTLSPVISGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDPADAKEMARLCASKEGMLVGISSGATLAAIAQKLPSLPAGSRVLGFNYDTGERYLSVPDFLPE

Sequences:

>Translated_305_residues
MRAESILATIGNTPHIRLSRLFPDHEVWVKCERANPGGSIKDRIGLAMIEAAEADGSLKPGGTIVEPTSGNTGIGLAMAA
AVKGYKLILVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAKEIVDQTDGAWMPQQFDNPANVSVHVRTTAQEIL
KDFGSEPIDVLITGVGTGGHLTGCAEELKKHWPSLKAYAVEPTLSPVISGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDPADAKEMARLCASKEGMLVGISSGATLAAIAQKLPSLPAGSRVLGFNYDTGERYLSVPDFLPE
>Mature_305_residues
MRAESILATIGNTPHIRLSRLFPDHEVWVKCERANPGGSIKDRIGLAMIEAAEADGSLKPGGTIVEPTSGNTGIGLAMAA
AVKGYKLILVMPESMSLERRRLMLAYGATFDLTPREKGMKGAIERAKEIVDQTDGAWMPQQFDNPANVSVHVRTTAQEIL
KDFGSEPIDVLITGVGTGGHLTGCAEELKKHWPSLKAYAVEPTLSPVISGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
VDPADAKEMARLCASKEGMLVGISSGATLAAIAQKLPSLPAGSRVLGFNYDTGERYLSVPDFLPE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=305, Percent_Identity=38.0327868852459, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI295821200, Length=305, Percent_Identity=38.0327868852459, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4557415, Length=305, Percent_Identity=38.0327868852459, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1788754, Length=305, Percent_Identity=48.8524590163934, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI2367138, Length=296, Percent_Identity=38.1756756756757, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=46.2295081967213, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17535051, Length=294, Percent_Identity=43.8775510204082, Blast_Score=214, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17562970, Length=295, Percent_Identity=42.0338983050847, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI32566674, Length=294, Percent_Identity=42.5170068027211, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17561720, Length=295, Percent_Identity=41.0169491525424, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17534315, Length=312, Percent_Identity=38.7820512820513, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25147552, Length=310, Percent_Identity=36.7741935483871, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32566672, Length=210, Percent_Identity=39.5238095238095, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17561716, Length=113, Percent_Identity=39.8230088495575, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=31.306990881459, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24643623, Length=306, Percent_Identity=35.9477124183007, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI20129101, Length=306, Percent_Identity=35.9477124183007, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32270; Mature: 32270

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAESILATIGNTPHIRLSRLFPDHEVWVKCERANPGGSIKDRIGLAMIEAAEADGSLKP
CCHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCC
GGTIVEPTSGNTGIGLAMAAAVKGYKLILVMPESMSLERRRLMLAYGATFDLTPREKGMK
CCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCHH
GAIERAKEIVDQTDGAWMPQQFDNPANVSVHVRTTAQEILKDFGSEPIDVLITGVGTGGH
HHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCC
LTGCAEELKKHWPSLKAYAVEPTLSPVISGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCEEE
VDPADAKEMARLCASKEGMLVGISSGATLAAIAQKLPSLPAGSRVLGFNYDTGERYLSVP
ECCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECC
DFLPE
CCCCC
>Mature Secondary Structure
MRAESILATIGNTPHIRLSRLFPDHEVWVKCERANPGGSIKDRIGLAMIEAAEADGSLKP
CCHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCC
GGTIVEPTSGNTGIGLAMAAAVKGYKLILVMPESMSLERRRLMLAYGATFDLTPREKGMK
CCEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHEEEEEECCEEECCCHHCCHH
GAIERAKEIVDQTDGAWMPQQFDNPANVSVHVRTTAQEILKDFGSEPIDVLITGVGTGGH
HHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECCCCCC
LTGCAEELKKHWPSLKAYAVEPTLSPVISGGQPGPHPIQGIGAGFIPGNLHTQSIDGAIQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCHHHCCCCCCCCCCEECCCCCEEE
VDPADAKEMARLCASKEGMLVGISSGATLAAIAQKLPSLPAGSRVLGFNYDTGERYLSVP
ECCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECC
DFLPE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867384 [H]