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The map label for this gene is gshB [H]

Identifier: 87200097

GI number: 87200097

Start: 2219246

End: 2220193

Strand: Reverse

Name: gshB [H]

Synonym: Saro_2081

Alternate gene names: 87200097

Gene position: 2220193-2219246 (Counterclockwise)

Preceding gene: 87200099

Following gene: 87200096

Centisome position: 62.34

GC content: 64.66

Gene sequence:

>948_bases
ATGACACTTCGCGTCGCGGTCCAGATGGACCCGCTCCATTCGATCAACATCGCCGGCGATTCGAGCTTTGCGCTGATGCT
TTCGGCGCAGGAGCGCGGACACGAGGTCTATCACTATGACGTCGGTTCGTTGTCGCTCGACGAGGACGACCGGTTGATCG
CTCATGCGAACCCGGTGACGGTCCAGCGGATCGAGGGCAACCATTTCCAGACCGGCGAGCGCCGGCGACTCGACCTGGGG
CGCGACATCGACGTGGTGCTGATGCGTCAGGACCCGCCGTTCCACATGGGCTACATCACGGCCACGCACCTGCTGGAGCG
GATCGAGAGCGAGACGCTGGTGGTCAACAACCCGCGCAGCGTGCGCAACGCGCCCGAGAAGGTCATGGTGCTCGACTACC
GGCGCTTCATGCCGCCGACGCTGGTCACGCGCTCGGTCGAGGAGGTGCGCAGCTTCCAGAAGAAGCACGGCGCCATCGTG
ATCAAGCCGATTCACGGCAACGGCGGCAAGGCCATCTTCCGGGTGAGCGAGGAGGGCGAGAACCTTGGCGCGCTGTTCGA
GGTGTTCAACCAGACCTGGCCCGAACCACACATGGTGCAGGCGTTCCTTCCCGAAGTGGCCAAGGGCGACAAGCGGATCG
TGCTGGTCGACGGCGAGATCGCCGGCGCGATCAACCGCAAGCCGGGCGTGGGCGAGTTCCGCTCGAACCTCGCGGTCGGC
GGCTATGCCGAAAAGACCGAACTGACGCAGGAGGAGCAGGAAATCTGCGCCGCGCTCGGGCCGGAGCTGAAGCGGCTTGG
GCTGATCTTCGTCGGCATCGACGTGATCGGCGGCAAGTACCTGACCGAAATCAACGTGACTTCGCCGACGGGCATCGTCG
CCATCGACAAGTTCAACGGCACCGATACGCCCGGGATGATCTGGGACGCGATCGAGGCCCGGCTCTAG

Upstream 100 bases:

>100_bases
AAGGTGTGGCGCCATCGTGGCGCGGGGCGAAAATGGTCGCGCAGCCGGTAATTCGGCTTTAAGGCGCCGGTTAGAGAAAT
ACCTGAGGCTGAGCACAAGC

Downstream 100 bases:

>100_bases
TCCTGCGGCTTTTCCTTCGCCCGGCAGGTAACGACGCGGGTGGATCGAAAGAACCTCGCGGCGCGTTGGCAGACCCATGA
ACGACTGGATCATCCACCTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTLRVAVQMDPLHSINIAGDSSFALMLSAQERGHEVYHYDVGSLSLDEDDRLIAHANPVTVQRIEGNHFQTGERRRLDLG
RDIDVVLMRQDPPFHMGYITATHLLERIESETLVVNNPRSVRNAPEKVMVLDYRRFMPPTLVTRSVEEVRSFQKKHGAIV
IKPIHGNGGKAIFRVSEEGENLGALFEVFNQTWPEPHMVQAFLPEVAKGDKRIVLVDGEIAGAINRKPGVGEFRSNLAVG
GYAEKTELTQEEQEICAALGPELKRLGLIFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPGMIWDAIEARL

Sequences:

>Translated_315_residues
MTLRVAVQMDPLHSINIAGDSSFALMLSAQERGHEVYHYDVGSLSLDEDDRLIAHANPVTVQRIEGNHFQTGERRRLDLG
RDIDVVLMRQDPPFHMGYITATHLLERIESETLVVNNPRSVRNAPEKVMVLDYRRFMPPTLVTRSVEEVRSFQKKHGAIV
IKPIHGNGGKAIFRVSEEGENLGALFEVFNQTWPEPHMVQAFLPEVAKGDKRIVLVDGEIAGAINRKPGVGEFRSNLAVG
GYAEKTELTQEEQEICAALGPELKRLGLIFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPGMIWDAIEARL
>Mature_314_residues
TLRVAVQMDPLHSINIAGDSSFALMLSAQERGHEVYHYDVGSLSLDEDDRLIAHANPVTVQRIEGNHFQTGERRRLDLGR
DIDVVLMRQDPPFHMGYITATHLLERIESETLVVNNPRSVRNAPEKVMVLDYRRFMPPTLVTRSVEEVRSFQKKHGAIVI
KPIHGNGGKAIFRVSEEGENLGALFEVFNQTWPEPHMVQAFLPEVAKGDKRIVLVDGEIAGAINRKPGVGEFRSNLAVGG
YAEKTELTQEEQEICAALGPELKRLGLIFVGIDVIGGKYLTEINVTSPTGIVAIDKFNGTDTPGMIWDAIEARL

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=43.1746031746032, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34971; Mature: 34839

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRVAVQMDPLHSINIAGDSSFALMLSAQERGHEVYHYDVGSLSLDEDDRLIAHANPVT
CEEEEEEEECCCEEEEECCCCCEEEEEEEHHCCCEEEEEECCCEEECCCCCEEEECCCEE
VQRIEGNHFQTGERRRLDLGRDIDVVLMRQDPPFHMGYITATHLLERIESETLVVNNPRS
EEEECCCCCCCCCCEECCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCHH
VRNAPEKVMVLDYRRFMPPTLVTRSVEEVRSFQKKHGAIVIKPIHGNGGKAIFRVSEEGE
HCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCC
NLGALFEVFNQTWPEPHMVQAFLPEVAKGDKRIVLVDGEIAGAINRKPGVGEFRSNLAVG
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHHCCEEC
GYAEKTELTQEEQEICAALGPELKRLGLIFVGIDVIGGKYLTEINVTSPTGIVAIDKFNG
CCCHHHHHHHHHHHHHHHHCHHHHHCEEEEEEEEEECCEEEEEEEECCCCCEEEEECCCC
TDTPGMIWDAIEARL
CCCCCHHHHHHHHCC
>Mature Secondary Structure 
TLRVAVQMDPLHSINIAGDSSFALMLSAQERGHEVYHYDVGSLSLDEDDRLIAHANPVT
EEEEEEEECCCEEEEECCCCCEEEEEEEHHCCCEEEEEECCCEEECCCCCEEEECCCEE
VQRIEGNHFQTGERRRLDLGRDIDVVLMRQDPPFHMGYITATHLLERIESETLVVNNPRS
EEEECCCCCCCCCCEECCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCEEEEECCHH
VRNAPEKVMVLDYRRFMPPTLVTRSVEEVRSFQKKHGAIVIKPIHGNGGKAIFRVSEEGE
HCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCC
NLGALFEVFNQTWPEPHMVQAFLPEVAKGDKRIVLVDGEIAGAINRKPGVGEFRSNLAVG
CHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHHCCEEC
GYAEKTELTQEEQEICAALGPELKRLGLIFVGIDVIGGKYLTEINVTSPTGIVAIDKFNG
CCCHHHHHHHHHHHHHHHHCHHHHHCEEEEEEEEEECCEEEEEEEECCCCCEEEEECCCC
TDTPGMIWDAIEARL
CCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA