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The map label for this gene is epsL [H]

Identifier: 87200065

GI number: 87200065

Start: 2189261

End: 2190664

Strand: Reverse

Name: epsL [H]

Synonym: Saro_2049

Alternate gene names: 87200065

Gene position: 2190664-2189261 (Counterclockwise)

Preceding gene: 87200066

Following gene: 87200064

Centisome position: 61.51

GC content: 63.46

Gene sequence:

>1404_bases
ATGGCAAGCGCCGGGCAGATGATCCGCCTGTTCAAACACTATATACCGCATTCGGTCCTGCTGCTGGGGCTGCTGGATTT
CATCCTGCTGCTCGGCGCGGGCGAGATCGGCTGGCAGCTTCGCGCCCACCAGATCGGCATCGATTCCGGCCAGTTCGGCA
TCCGCCTGACGCCGCTGCTGCTGTTCGCGGGGCTGGTGCAGACCGCGATGATCGCGGTCGGCGTCTATGGTTCGGATGCC
CTGCGTTCGATGCGCTATGCGACCGCGCGCCTGCTGGTCGCCGTAAGCCTTGGCATCATTGCGCTGTCGGTCGTCTATTT
CATGCTGCCGGGGCGGACGTTGTGGCGTTCGAATCTGCTCTACGCGATGTTCCTGGCCATGGGGATGCTCGTGCTCATCC
GCCTCCTTCTGGGCGGATTGCTGGGCACGTCGGCCTTCCGCAGGCGCGTCCTGGTCCTTGGCGCGGGAGCGCGGGCCGAA
AGGCTGCGCAAGCTCGGAGAGAGGCCTGAAGCCGGTTTCGCCATCGTCGGCTACATCGGCATGAGCAGCGCTGCACCGAC
GGTCGTCGAGGCGATACATCGCGATGCGATCAACAACCTGACGCGCTACGTCGAGAACCTTGGCGTCAGCGAGGTGGTCC
TCGCGCTCGAGGAGCGGCGGAACGCCTTGCCGCTCAAGGATCTTCTCAGGATCAAGACCACCGGCGTCCACGTCAACGAC
TTCTCCTCCTTCATGGAGCGAGAGACGGGCCGCGTGGACCTCGACACGGTCAATCCGAGCTGGCTGATCTTTTCAGACGG
GTTCTCATCGGGCAGGGCGCTGTCGAGCGTGGCAAAGCGCATCTTCGACATTGGCGCGAGCCTGCTGCTGCTTGTCGCCA
CGTTCCCGGTCATCCTCCTGTTCGCGATGCTGGTGAAGCTCGACAGCAAGGGCCCTGCGTTCTTCCGCCAGACGCGCGTT
GGCCTCTACGGTCAGCCGTTCGACCTCATCAAGCTGCGTTCGATGCGCATGGATGCGGAAGCCAACGGGGCGCAGTTCGC
GCAGAAGGACGATCCTCGCGTGACCCGCATCGGCCGGATCATCCGCAAGCTGCGGATCGATGAACTGCCGCAGGCCTGGA
CGGTGCTGAAAGGCGAGATGAGCTTCGTCGGGCCGCGCCCGGAACGTCCCGAGTTCGTGGCCGACCTGGAAGACAAGCTG
CCTTATTATGCCGAGCGCCACATGGTGAAGCCAGGCATCACTGGCTGGGCGCAGATCAACTACCCCTATGGCGCGTCCAT
CGAGGATTCACGGCACAAGCTCGAATACGACCTCTACTACGCCAAGAACTACACCCCCTTTCTCGATCTCCTGATCCTGC
TCCAGACCTTGCGCGTCGTGCTGTGGCACGAAGGCGCGCGGTGA

Upstream 100 bases:

>100_bases
TATACCCGTCTCAAATGGCGTTTGAATTGTCAGGGTAACCGACGCTTTACCCTTTGCGCAGTATGCCCTTGCCCGTGGGA
GCCTGCCGGCTTCCAAGGGG

Downstream 100 bases:

>100_bases
AAGGCGTGCCATGCTCAATCCACTGACCTGGGAAGGCTTCGGCGAGTGGGCGTTCATCGCCTCGGCATTCGCGTTCCTGA
TGCTGGCCGCGTGGCGCTGG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MASAGQMIRLFKHYIPHSVLLLGLLDFILLLGAGEIGWQLRAHQIGIDSGQFGIRLTPLLLFAGLVQTAMIAVGVYGSDA
LRSMRYATARLLVAVSLGIIALSVVYFMLPGRTLWRSNLLYAMFLAMGMLVLIRLLLGGLLGTSAFRRRVLVLGAGARAE
RLRKLGERPEAGFAIVGYIGMSSAAPTVVEAIHRDAINNLTRYVENLGVSEVVLALEERRNALPLKDLLRIKTTGVHVND
FSSFMERETGRVDLDTVNPSWLIFSDGFSSGRALSSVAKRIFDIGASLLLLVATFPVILLFAMLVKLDSKGPAFFRQTRV
GLYGQPFDLIKLRSMRMDAEANGAQFAQKDDPRVTRIGRIIRKLRIDELPQAWTVLKGEMSFVGPRPERPEFVADLEDKL
PYYAERHMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVVLWHEGAR

Sequences:

>Translated_467_residues
MASAGQMIRLFKHYIPHSVLLLGLLDFILLLGAGEIGWQLRAHQIGIDSGQFGIRLTPLLLFAGLVQTAMIAVGVYGSDA
LRSMRYATARLLVAVSLGIIALSVVYFMLPGRTLWRSNLLYAMFLAMGMLVLIRLLLGGLLGTSAFRRRVLVLGAGARAE
RLRKLGERPEAGFAIVGYIGMSSAAPTVVEAIHRDAINNLTRYVENLGVSEVVLALEERRNALPLKDLLRIKTTGVHVND
FSSFMERETGRVDLDTVNPSWLIFSDGFSSGRALSSVAKRIFDIGASLLLLVATFPVILLFAMLVKLDSKGPAFFRQTRV
GLYGQPFDLIKLRSMRMDAEANGAQFAQKDDPRVTRIGRIIRKLRIDELPQAWTVLKGEMSFVGPRPERPEFVADLEDKL
PYYAERHMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVVLWHEGAR
>Mature_466_residues
ASAGQMIRLFKHYIPHSVLLLGLLDFILLLGAGEIGWQLRAHQIGIDSGQFGIRLTPLLLFAGLVQTAMIAVGVYGSDAL
RSMRYATARLLVAVSLGIIALSVVYFMLPGRTLWRSNLLYAMFLAMGMLVLIRLLLGGLLGTSAFRRRVLVLGAGARAER
LRKLGERPEAGFAIVGYIGMSSAAPTVVEAIHRDAINNLTRYVENLGVSEVVLALEERRNALPLKDLLRIKTTGVHVNDF
SSFMERETGRVDLDTVNPSWLIFSDGFSSGRALSSVAKRIFDIGASLLLLVATFPVILLFAMLVKLDSKGPAFFRQTRVG
LYGQPFDLIKLRSMRMDAEANGAQFAQKDDPRVTRIGRIIRKLRIDELPQAWTVLKGEMSFVGPRPERPEFVADLEDKLP
YYAERHMVKPGITGWAQINYPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVVLWHEGAR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=398, Percent_Identity=30.4020100502513, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52094; Mature: 51963

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAGQMIRLFKHYIPHSVLLLGLLDFILLLGAGEIGWQLRAHQIGIDSGQFGIRLTPLL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEHHHHH
LFAGLVQTAMIAVGVYGSDALRSMRYATARLLVAVSLGIIALSVVYFMLPGRTLWRSNLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YAMFLAMGMLVLIRLLLGGLLGTSAFRRRVLVLGAGARAERLRKLGERPEAGFAIVGYIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCCEEEEEECC
MSSAAPTVVEAIHRDAINNLTRYVENLGVSEVVLALEERRNALPLKDLLRIKTTGVHVND
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
FSSFMERETGRVDLDTVNPSWLIFSDGFSSGRALSSVAKRIFDIGASLLLLVATFPVILL
HHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAMLVKLDSKGPAFFRQTRVGLYGQPFDLIKLRSMRMDAEANGAQFAQKDDPRVTRIGRI
HHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
IRKLRIDELPQAWTVLKGEMSFVGPRPERPEFVADLEDKLPYYAERHMVKPGITGWAQIN
HHHHHHHCCCHHHHHHHCCHHCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEEC
YPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVVLWHEGAR
CCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASAGQMIRLFKHYIPHSVLLLGLLDFILLLGAGEIGWQLRAHQIGIDSGQFGIRLTPLL
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEHHHHH
LFAGLVQTAMIAVGVYGSDALRSMRYATARLLVAVSLGIIALSVVYFMLPGRTLWRSNLL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YAMFLAMGMLVLIRLLLGGLLGTSAFRRRVLVLGAGARAERLRKLGERPEAGFAIVGYIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCCEEEEEECC
MSSAAPTVVEAIHRDAINNLTRYVENLGVSEVVLALEERRNALPLKDLLRIKTTGVHVND
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
FSSFMERETGRVDLDTVNPSWLIFSDGFSSGRALSSVAKRIFDIGASLLLLVATFPVILL
HHHHHHHCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAMLVKLDSKGPAFFRQTRVGLYGQPFDLIKLRSMRMDAEANGAQFAQKDDPRVTRIGRI
HHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
IRKLRIDELPQAWTVLKGEMSFVGPRPERPEFVADLEDKLPYYAERHMVKPGITGWAQIN
HHHHHHHCCCHHHHHHHCCHHCCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCEEEEC
YPYGASIEDSRHKLEYDLYYAKNYTPFLDLLILLQTLRVVLWHEGAR
CCCCCCCCCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]