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The map label for this gene is panB

Identifier: 87200059

GI number: 87200059

Start: 2181011

End: 2181880

Strand: Direct

Name: panB

Synonym: Saro_2043

Alternate gene names: 87200059

Gene position: 2181011-2181880 (Clockwise)

Preceding gene: 87200057

Following gene: 87200060

Centisome position: 61.24

GC content: 65.98

Gene sequence:

>870_bases
ATGTCCACGACCTTCCAGCTCGATACCTCGACAAGCCGTGCCAACCCCACCCCGGCACCGATGAAGCGCCTGACGATCCC
CGCGATCCGCCAGCGCAAGAAGGACGGCGTGACGGCACAACCGGTCGTGATGCTCACCGCCTATACCGCCCGTCAGGCGC
AATTGCTCGACGCCCATTGCGATCTGCTGCTGGTCGGGGATTCCCTTGGCCAGGTGATCTATGGCCTGCCTTCCAGCGTT
CCGGTCACGCTCGACATGATGGCAGCCCATGGCGCGGCGGTCGTGCGTGGATCGTATCACGCGGCGGTCGTGGTCGACAT
GCCGTTCGGCAGCTACGAAGCATCGCCGCAGCAGGCCTTCGAAAGCGCCTCGCGGCTGCTGAAGGAAACCGGTTGCGCGG
CAGTGAAGCTCGAAGGGGGCGAGGCCATGGCCGAAACGGTCGCGTTCCTCACCAACCGGGGCATCCCGGTCATGGGCCAC
GTCGGCCTCACCCCGCAGGCGGTCAATGTGCTGGGCGGCTACAACGCGCGCGGCCGCAGCGAGGCCGAAGCGGCCAAGAT
CGTCGGAGACGCACGGTCGCTTGCCGATGCCGGTGCCTTCGCCATCGTGATCGAAGGCGTGGTCGAGCCCATCGCGATTG
CCATCACCCAGGCCGTGGCGTGCCCGACAATCGGCATCGGCGCCTCGGCTCAATGCGACGGGCAGGTTCTGGTCACCGAA
GACATGCTCGGCATGTTCGAGCGCGTTCCGCGGTTCGTGAAGATCTACGAGGATCTCGCCGGGACCATCTCCGGGGCTGC
GGCGCGTTATGCCGACGAAGTGCGCGCGCGCACCTTCCCCGGTATTGAACAGACGTACCAGCCCAAGTAA

Upstream 100 bases:

>100_bases
GCAAGCCATTTTTCCCCAAGATTGCGCGTGTGCAGCGCCAGCCTTGCGGCTTTCTGACAAGGCAGTAGAAGCATCAGCCC
GCCTGAAAGGATGCGCGCCC

Downstream 100 bases:

>100_bases
CCGTCACGCCGAGGTAACCGTCACGGCCAGGCCATCGTCACACCTCGGCCGGCACACCAAGACAGACAGGGAATTCCGCT
TGCTCCGCTTCTACAAGGGT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTTFQLDTSTSRANPTPAPMKRLTIPAIRQRKKDGVTAQPVVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSSV
PVTLDMMAAHGAAVVRGSYHAAVVVDMPFGSYEASPQQAFESASRLLKETGCAAVKLEGGEAMAETVAFLTNRGIPVMGH
VGLTPQAVNVLGGYNARGRSEAEAAKIVGDARSLADAGAFAIVIEGVVEPIAIAITQAVACPTIGIGASAQCDGQVLVTE
DMLGMFERVPRFVKIYEDLAGTISGAAARYADEVRARTFPGIEQTYQPK

Sequences:

>Translated_289_residues
MSTTFQLDTSTSRANPTPAPMKRLTIPAIRQRKKDGVTAQPVVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSSV
PVTLDMMAAHGAAVVRGSYHAAVVVDMPFGSYEASPQQAFESASRLLKETGCAAVKLEGGEAMAETVAFLTNRGIPVMGH
VGLTPQAVNVLGGYNARGRSEAEAAKIVGDARSLADAGAFAIVIEGVVEPIAIAITQAVACPTIGIGASAQCDGQVLVTE
DMLGMFERVPRFVKIYEDLAGTISGAAARYADEVRARTFPGIEQTYQPK
>Mature_288_residues
STTFQLDTSTSRANPTPAPMKRLTIPAIRQRKKDGVTAQPVVMLTAYTARQAQLLDAHCDLLLVGDSLGQVIYGLPSSVP
VTLDMMAAHGAAVVRGSYHAAVVVDMPFGSYEASPQQAFESASRLLKETGCAAVKLEGGEAMAETVAFLTNRGIPVMGHV
GLTPQAVNVLGGYNARGRSEAEAAKIVGDARSLADAGAFAIVIEGVVEPIAIAITQAVACPTIGIGASAQCDGQVLVTED
MLGMFERVPRFVKIYEDLAGTISGAAARYADEVRARTFPGIEQTYQPK

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=269, Percent_Identity=40.5204460966543, Blast_Score=189, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319653, Length=285, Percent_Identity=38.2456140350877, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_NOVAD (Q2G6P1)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497316.1
- ProteinModelPortal:   Q2G6P1
- SMR:   Q2G6P1
- STRING:   Q2G6P1
- GeneID:   3917690
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2043
- NMPDR:   fig|48935.1.peg.730
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- PhylomeDB:   Q2G6P1
- ProtClustDB:   PRK00311
- BioCyc:   NARO279238:SARO_2043-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 30324; Mature: 30193

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: ACT_SITE 205-205 BINDING 106-106 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTFQLDTSTSRANPTPAPMKRLTIPAIRQRKKDGVTAQPVVMLTAYTARQAQLLDAHC
CCCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCC
DLLLVGDSLGQVIYGLPSSVPVTLDMMAAHGAAVVRGSYHAAVVVDMPFGSYEASPQQAF
CEEEECCHHHHHHHCCCCCCCEEEEHHHHCCCEEEECCCEEEEEEECCCCCCCCCHHHHH
ESASRLLKETGCAAVKLEGGEAMAETVAFLTNRGIPVMGHVGLTPQAVNVLGGYNARGRS
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCC
EAEAAKIVGDARSLADAGAFAIVIEGVVEPIAIAITQAVACPTIGIGASAQCDGQVLVTE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHH
DMLGMFERVPRFVKIYEDLAGTISGAAARYADEVRARTFPGIEQTYQPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STTFQLDTSTSRANPTPAPMKRLTIPAIRQRKKDGVTAQPVVMLTAYTARQAQLLDAHC
CCEEEEECCCCCCCCCCCCHHHCCCHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHCCC
DLLLVGDSLGQVIYGLPSSVPVTLDMMAAHGAAVVRGSYHAAVVVDMPFGSYEASPQQAF
CEEEECCHHHHHHHCCCCCCCEEEEHHHHCCCEEEECCCEEEEEEECCCCCCCCCHHHHH
ESASRLLKETGCAAVKLEGGEAMAETVAFLTNRGIPVMGHVGLTPQAVNVLGGYNARGRS
HHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCC
EAEAAKIVGDARSLADAGAFAIVIEGVVEPIAIAITQAVACPTIGIGASAQCDGQVLVTE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHH
DMLGMFERVPRFVKIYEDLAGTISGAAARYADEVRARTFPGIEQTYQPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA