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The map label for this gene is ccsA [H]

Identifier: 87200048

GI number: 87200048

Start: 2167442

End: 2168728

Strand: Direct

Name: ccsA [H]

Synonym: Saro_2032

Alternate gene names: 87200048

Gene position: 2167442-2168728 (Clockwise)

Preceding gene: 87200047

Following gene: 87200049

Centisome position: 60.86

GC content: 62.86

Gene sequence:

>1287_bases
GTGAGCGATAACCAGGCGTCTTTGACCGCTGACGGCAAGACCGTGGAAATGCCGGTCAAGAGCGGCACGATCGGGCCGGA
TGTCATCGATATCCGCAAGCTCTATGGCCAGACGGGCATGTTCACCTACGACCCGGGCTTCACCTCGACGGCGAGCTGCG
ATTCCGCGCTCACCTACATCGATGGCGACGAGGGCGTGCTGCTCCACCGCGGCTATCCGATCGGTCAGCTCGCCGAACAT
TCGTCGTTCATGGAAGTGAGCTACCTGCTGCTCAACGGCGAACTGCCGAGCCAGCAGGAACTGGCGGACTTCACCCGCAC
GATCACCCGCCACACCATGCTGCACGAACAGCTTGCGACGTTCTATCGCGGCTTCCGCCGTGATGCGCACCCGATGGCGA
TCATGTGCGGCGTGGTCGGCGCGCTTTCGGCGTTCTATCACGACAGCACCGACATTTCCGATCCGACGCACCGCAAGATC
AGCTCGCACCGCCTGATCGCGAAGATGCCGACGATCGCGGCAATGGCCTACAAGTATTCGGTCGGCCAGCCCTTCGTCTA
TCCGGACAACAAGCTGTCGTACACCGGCAACTTCCTGAAGATGACCTTCGGCGTCCCCGCCGAGGAATATGAAGTGATCC
CCGCCGTCGAAAAGGCGATGGACCGCATCTTCATCCTCCACGCCGACCACGAGCAGAACGCCTCGACGTCGACCGTGCGA
CTGGCCGGATCGTCGGGCGCCAATCCGTTCGCGTGCATCGCGGCCGGCATTGCCTGCCTGTGGGGCCCTGCGCATGGCGG
CGCCAACGAAGCCGCGCTGAACATGCTCAAGGAAATCGGCACTCCGGACAAGATCCCGCACTACATCGAGCGTGCGAAGG
ACAAGAACGATCCGTTCCGCCTGATGGGCTTCGGTCACCGCGTCTACAAGAACTACGACCCGCGCGCGACCGTGATGCAG
CAGACCGTGCGCGAAGTGTTCGACGCGCTGAAGGTCAACGATCCGCTGTTCGAGACCGCGCTGCGCCTCGAAGAGATCGC
GCTGAACGACCCGTACTTCGTCGAGAAGAAGCTGTTCCCGAACGTCGACTTCTATTCGGGCATCATCCTGTCGGCGATCG
GCTTCCCGACGACGATGTTCACCGTGCTCTTCGCGCTGGCCCGCACCGTGGGCTGGGTTGCGCAGTGGAACGAAATGATC
AGCGATCCCGGCCAGAAGATCGGCCGTCCGCGCCAGCTCTACACCGGCCCGACGCAGCGCGATTACGTGCCGCTGGCTAG
CCGCTGA

Upstream 100 bases:

>100_bases
TGGCGCTGCTCGGCAAGGACGAGTCGCTGTCGCGCATTCGCGATCAGGCGGCCTGACCGGGCAAGCTATTGCAAAGACAC
AACGAACAGGAGGGCTAAGC

Downstream 100 bases:

>100_bases
GGCGTCTACGCGCGGGTTGTCCGGGCCGGTTTCTCGAGAGAAACCGGTCGGGCCAGCTCTACACTGGCCCGACGCAGCGC
GATTACGTGCCGCTGGCTAG

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSDNQASLTADGKTVEMPVKSGTIGPDVIDIRKLYGQTGMFTYDPGFTSTASCDSALTYIDGDEGVLLHRGYPIGQLAEH
SSFMEVSYLLLNGELPSQQELADFTRTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPTHRKI
SSHRLIAKMPTIAAMAYKYSVGQPFVYPDNKLSYTGNFLKMTFGVPAEEYEVIPAVEKAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLKEIGTPDKIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQ
QTVREVFDALKVNDPLFETALRLEEIALNDPYFVEKKLFPNVDFYSGIILSAIGFPTTMFTVLFALARTVGWVAQWNEMI
SDPGQKIGRPRQLYTGPTQRDYVPLASR

Sequences:

>Translated_428_residues
MSDNQASLTADGKTVEMPVKSGTIGPDVIDIRKLYGQTGMFTYDPGFTSTASCDSALTYIDGDEGVLLHRGYPIGQLAEH
SSFMEVSYLLLNGELPSQQELADFTRTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPTHRKI
SSHRLIAKMPTIAAMAYKYSVGQPFVYPDNKLSYTGNFLKMTFGVPAEEYEVIPAVEKAMDRIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLKEIGTPDKIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQ
QTVREVFDALKVNDPLFETALRLEEIALNDPYFVEKKLFPNVDFYSGIILSAIGFPTTMFTVLFALARTVGWVAQWNEMI
SDPGQKIGRPRQLYTGPTQRDYVPLASR
>Mature_427_residues
SDNQASLTADGKTVEMPVKSGTIGPDVIDIRKLYGQTGMFTYDPGFTSTASCDSALTYIDGDEGVLLHRGYPIGQLAEHS
SFMEVSYLLLNGELPSQQELADFTRTITRHTMLHEQLATFYRGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPTHRKIS
SHRLIAKMPTIAAMAYKYSVGQPFVYPDNKLSYTGNFLKMTFGVPAEEYEVIPAVEKAMDRIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALNMLKEIGTPDKIPHYIERAKDKNDPFRLMGFGHRVYKNYDPRATVMQQ
TVREVFDALKVNDPLFETALRLEEIALNDPYFVEKKLFPNVDFYSGIILSAIGFPTTMFTVLFALARTVGWVAQWNEMIS
DPGQKIGRPRQLYTGPTQRDYVPLASR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=355, Percent_Identity=29.0140845070423, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1786939, Length=423, Percent_Identity=68.0851063829787, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1786527, Length=380, Percent_Identity=32.8947368421053, Blast_Score=174, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17555174, Length=399, Percent_Identity=26.8170426065163, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=356, Percent_Identity=27.5280898876405, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=352, Percent_Identity=27.5568181818182, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325257, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640124, Length=453, Percent_Identity=28.476821192053, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640126, Length=454, Percent_Identity=28.4140969162996, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21356863, Length=354, Percent_Identity=27.4011299435028, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47508; Mature: 47376

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQASLTADGKTVEMPVKSGTIGPDVIDIRKLYGQTGMFTYDPGFTSTASCDSALTYI
CCCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
DGDEGVLLHRGYPIGQLAEHSSFMEVSYLLLNGELPSQQELADFTRTITRHTMLHEQLAT
ECCCCEEEECCCCHHHHHHCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FYRGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPTHRKISSHRLIAKMPTIAAMAYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHCCHHHHHHHHHC
VGQPFVYPDNKLSYTGNFLKMTFGVPAEEYEVIPAVEKAMDRIFILHADHEQNASTSTVR
CCCCEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLKEIGTPDKIPHYIERAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRATVMQQTVREVFDALKVNDPLFETALRLEEIALNDPYFVEKKLFP
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHCC
NVDFYSGIILSAIGFPTTMFTVLFALARTVGWVAQWNEMISDPGQKIGRPRQLYTGPTQR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCC
DYVPLASR
CCCCCCCC
>Mature Secondary Structure 
SDNQASLTADGKTVEMPVKSGTIGPDVIDIRKLYGQTGMFTYDPGFTSTASCDSALTYI
CCCCCEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEE
DGDEGVLLHRGYPIGQLAEHSSFMEVSYLLLNGELPSQQELADFTRTITRHTMLHEQLAT
ECCCCEEEECCCCHHHHHHCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
FYRGFRRDAHPMAIMCGVVGALSAFYHDSTDISDPTHRKISSHRLIAKMPTIAAMAYKYS
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHHHCCHHHHHHHHHC
VGQPFVYPDNKLSYTGNFLKMTFGVPAEEYEVIPAVEKAMDRIFILHADHEQNASTSTVR
CCCCEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLKEIGTPDKIPHYIERAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRATVMQQTVREVFDALKVNDPLFETALRLEEIALNDPYFVEKKLFP
EEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHCC
NVDFYSGIILSAIGFPTTMFTVLFALARTVGWVAQWNEMISDPGQKIGRPRQLYTGPTQR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEECCCCCCC
DYVPLASR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]