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The map label for this gene is pyrG

Identifier: 87200033

GI number: 87200033

Start: 2149283

End: 2150914

Strand: Reverse

Name: pyrG

Synonym: Saro_2017

Alternate gene names: 87200033

Gene position: 2150914-2149283 (Counterclockwise)

Preceding gene: 87200034

Following gene: 87200032

Centisome position: 60.39

GC content: 65.38

Gene sequence:

>1632_bases
ATGGCGCGGTACATATTCATCACCGGCGGCGTGGTTTCCTCGCTCGGCAAGGGACTCATGGCAGCGAGCCTCGCGGCTCT
GTTGCAGGCACGCGGATTTCGCGTGCGCATCCGCAAGTTCGACCCCTATCTGAACGTCGATCCGGGGACGATGAGCCCCT
ATCAGCACGGCGAGGTCTACGTGACCGACGACGGCGCCGAGACCGACCTCGACCTCGGCCACTACGAACGCTTCACCGGC
GTTTCGGCGCGGCAGGCGGACAACATCACTTCGGGCCGCATCTATCGCGATATCATCACGAAGGAGCGGCGCGGCGACTA
TCTCGGCGCGACGGTCCAGGTGATTCCGCACGTGACCGATGCGATCAAGGACTTCGCCCAGGCCGAGACCGAGGATCTCG
ACTTCGTGCTGTGCGAGATCGGCGGCACCGTGGGCGACATCGAGGGTCTGCCCTTCATCGAGGCGCTGCGTCAGCTCCAC
AACGAGCTTGACCGCGACCAGACCTGTTTCGTCCACGTGACGCTGGTGCCCTACATCGCGGCGGCGGGCGAGCTGAAGAC
CAAGCCGACCCAGCATTCCGTGCGCGAACTGACCGGGCTTGGCATCCAGCCGGACATCCTGCTTTGCCGCTGCGAGAAGC
CGCTTCCGGAGGGTGAGCGCGCCAAGATCGCGCAGTTCTGCAACGTGCGCAAATCGGCGGTCATTCCCGCGCTCGACGCT
TCGAGCATCTATGCGGTGCCGTTGCAGTATCACGCCGAGGGGCTGGACGGCGAAGTGCTGCGCCACTTCGGCCTGACCGC
TCCGGACCCGGACCTGGCGCGGTGGGAAGACATCGTCGACCGCTACCAGAACCCCGAGGGCGAAGTGACGATCGGCGTGG
TCGGCAAGTATGTCGGCCTGCAGGACGCCTACAAGTCGCTCAACGAGGCGCTGGCGCATGGCGGCATGGCCAATCGCGTC
AAGGTGCGGGTCAAGTGGCTCGACGCCGAGCTTTTCGAGAAGGGCGACGACGAGATCGCCGCGCAGCTCGAGCCGATGCA
CGGCATTCTCGTGCCCGGCGGCTTCGGCGAGCGCGGGACCGAAGGCAAGATCGCCTCGGTGCGCTTTGCGCGCGAACGCA
AGGTGCCGTTCTTCGGCATCTGCCTCGGCATGCAGATGGCCTGCGTCGAGGGGGCCCGCAACACGGCGGGGATCGCGGGC
GCTTCGTCGACAGAGTTCGGGCCGACCGACGAGCCGGTCGTGGGCATCATCACCGAGTGGATGACCGCCGAAGGGCTTGA
GAAACGCTCCGAAGGCGGGGATCTGGGCGGCACCATGCGCCTGGGCGCGTATGAGGCGCACCTTGCAGGCAACAGTCACG
TCGCCAACATCTATGGCAGCACCGTGATCAGCGAACGGCATCGCCACCGCTACGAAGTGAACGTCGCCTACAAGGAACGG
CTGGAAAAGGGCGGGCTGGTGTTTTCGGGCATGTCGCCCGACGGACTGCTCCCCGAGATCGTGGAGCGTCCCGACCATCC
GTGGTTCATCGGTGTGCAGTTCCATCCCGAACTGAAGAGCCGTCCGTTCGAGCCGCACCCGCTGTTCAAGGGGTTCATCG
CGGCCGCAGTCAAGCAGGCGCGTCTGGTTTAA

Upstream 100 bases:

>100_bases
GCTTTCGTGTTGTTTTCGATACCCGCTTGTGGATTGCCGCAAAGATTACAGCGGCAACCGCTTGCGTTCATGCAACCTCT
GACGCTAAGGCCCGACTCCC

Downstream 100 bases:

>100_bases
AAAACGTGAAGGGGCTGGCCAGAGCCCCGGTTTGCTGATTATTTGCCGCCGGCCGCGGCTGTCGCGGCAGGGGGCGAGAC
GGCGATGGACGCGGCGAGGC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MARYIFITGGVVSSLGKGLMAASLAALLQARGFRVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTG
VSARQADNITSGRIYRDIITKERRGDYLGATVQVIPHVTDAIKDFAQAETEDLDFVLCEIGGTVGDIEGLPFIEALRQLH
NELDRDQTCFVHVTLVPYIAAAGELKTKPTQHSVRELTGLGIQPDILLCRCEKPLPEGERAKIAQFCNVRKSAVIPALDA
SSIYAVPLQYHAEGLDGEVLRHFGLTAPDPDLARWEDIVDRYQNPEGEVTIGVVGKYVGLQDAYKSLNEALAHGGMANRV
KVRVKWLDAELFEKGDDEIAAQLEPMHGILVPGGFGERGTEGKIASVRFARERKVPFFGICLGMQMACVEGARNTAGIAG
ASSTEFGPTDEPVVGIITEWMTAEGLEKRSEGGDLGGTMRLGAYEAHLAGNSHVANIYGSTVISERHRHRYEVNVAYKER
LEKGGLVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAVKQARLV

Sequences:

>Translated_543_residues
MARYIFITGGVVSSLGKGLMAASLAALLQARGFRVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTG
VSARQADNITSGRIYRDIITKERRGDYLGATVQVIPHVTDAIKDFAQAETEDLDFVLCEIGGTVGDIEGLPFIEALRQLH
NELDRDQTCFVHVTLVPYIAAAGELKTKPTQHSVRELTGLGIQPDILLCRCEKPLPEGERAKIAQFCNVRKSAVIPALDA
SSIYAVPLQYHAEGLDGEVLRHFGLTAPDPDLARWEDIVDRYQNPEGEVTIGVVGKYVGLQDAYKSLNEALAHGGMANRV
KVRVKWLDAELFEKGDDEIAAQLEPMHGILVPGGFGERGTEGKIASVRFARERKVPFFGICLGMQMACVEGARNTAGIAG
ASSTEFGPTDEPVVGIITEWMTAEGLEKRSEGGDLGGTMRLGAYEAHLAGNSHVANIYGSTVISERHRHRYEVNVAYKER
LEKGGLVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAVKQARLV
>Mature_542_residues
ARYIFITGGVVSSLGKGLMAASLAALLQARGFRVRIRKFDPYLNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGV
SARQADNITSGRIYRDIITKERRGDYLGATVQVIPHVTDAIKDFAQAETEDLDFVLCEIGGTVGDIEGLPFIEALRQLHN
ELDRDQTCFVHVTLVPYIAAAGELKTKPTQHSVRELTGLGIQPDILLCRCEKPLPEGERAKIAQFCNVRKSAVIPALDAS
SIYAVPLQYHAEGLDGEVLRHFGLTAPDPDLARWEDIVDRYQNPEGEVTIGVVGKYVGLQDAYKSLNEALAHGGMANRVK
VRVKWLDAELFEKGDDEIAAQLEPMHGILVPGGFGERGTEGKIASVRFARERKVPFFGICLGMQMACVEGARNTAGIAGA
SSTEFGPTDEPVVGIITEWMTAEGLEKRSEGGDLGGTMRLGAYEAHLAGNSHVANIYGSTVISERHRHRYEVNVAYKERL
EKGGLVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAVKQARLV

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=45.9893048128342, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI28559085, Length=555, Percent_Identity=43.4234234234234, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI28559083, Length=555, Percent_Identity=43.4234234234234, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI221316689, Length=555, Percent_Identity=43.4234234234234, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=57.3556797020484, Blast_Score=606, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25148299, Length=609, Percent_Identity=36.6174055829228, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322563, Length=570, Percent_Identity=41.5789473684211, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=567, Percent_Identity=40.3880070546737, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=44.524236983842, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=43.5387673956262, Blast_Score=390, Evalue=1e-108,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_NOVAD (Q2G6R7)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497290.1
- HSSP:   P17812
- ProteinModelPortal:   Q2G6R7
- SMR:   Q2G6R7
- STRING:   Q2G6R7
- GeneID:   3917338
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2017
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q2G6R7
- ProtClustDB:   PRK05380
- BioCyc:   NARO279238:SARO_2017-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59507; Mature: 59376

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 515-515 ACT_SITE 517-517

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYIFITGGVVSSLGKGLMAASLAALLQARGFRVRIRKFDPYLNVDPGTMSPYQHGEVY
CCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARQADNITSGRIYRDIITKERRGDYLGATVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFAQAETEDLDFVLCEIGGTVGDIEGLPFIEALRQLHNELDRDQTCFVHVTLVPYIA
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHH
AAGELKTKPTQHSVRELTGLGIQPDILLCRCEKPLPEGERAKIAQFCNVRKSAVIPALDA
HCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
SSIYAVPLQYHAEGLDGEVLRHFGLTAPDPDLARWEDIVDRYQNPEGEVTIGVVGKYVGL
CCEEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCH
QDAYKSLNEALAHGGMANRVKVRVKWLDAELFEKGDDEIAAQLEPMHGILVPGGFGERGT
HHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCCHHHHHHCCCCCEEEECCCCCCCCC
EGKIASVRFARERKVPFFGICLGMQMACVEGARNTAGIAGASSTEFGPTDEPVVGIITEW
CCCEEEEEEHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
MTAEGLEKRSEGGDLGGTMRLGAYEAHLAGNSHVANIYGSTVISERHRHRYEVNVAYKER
HHHHHHHHHCCCCCCCCEEEECCEEEEECCCCEEEHHHHHHHHHHHHCCEEEEEEHHHHH
LEKGGLVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAVKQA
HHHCCEEEECCCCCCCCHHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
CCC
>Mature Secondary Structure 
ARYIFITGGVVSSLGKGLMAASLAALLQARGFRVRIRKFDPYLNVDPGTMSPYQHGEVY
CEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFTGVSARQADNITSGRIYRDIITKERRGDYLGATVQVIPHVTD
EECCCCCCCCCCCHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AIKDFAQAETEDLDFVLCEIGGTVGDIEGLPFIEALRQLHNELDRDQTCFVHVTLVPYIA
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHH
AAGELKTKPTQHSVRELTGLGIQPDILLCRCEKPLPEGERAKIAQFCNVRKSAVIPALDA
HCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCHHHHCCCCCCC
SSIYAVPLQYHAEGLDGEVLRHFGLTAPDPDLARWEDIVDRYQNPEGEVTIGVVGKYVGL
CCEEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHCCH
QDAYKSLNEALAHGGMANRVKVRVKWLDAELFEKGDDEIAAQLEPMHGILVPGGFGERGT
HHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHHCCCHHHHHHCCCCCEEEECCCCCCCCC
EGKIASVRFARERKVPFFGICLGMQMACVEGARNTAGIAGASSTEFGPTDEPVVGIITEW
CCCEEEEEEHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
MTAEGLEKRSEGGDLGGTMRLGAYEAHLAGNSHVANIYGSTVISERHRHRYEVNVAYKER
HHHHHHHHHCCCCCCCCEEEECCEEEEECCCCEEEHHHHHHHHHHHHCCEEEEEEHHHHH
LEKGGLVFSGMSPDGLLPEIVERPDHPWFIGVQFHPELKSRPFEPHPLFKGFIAAAVKQA
HHHCCEEEECCCCCCCCHHHHCCCCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHHHHH
RLV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA