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The map label for this gene is pdhB [H]

Identifier: 87199926

GI number: 87199926

Start: 2021459

End: 2022844

Strand: Direct

Name: pdhB [H]

Synonym: Saro_1909

Alternate gene names: 87199926

Gene position: 2021459-2022844 (Clockwise)

Preceding gene: 87199925

Following gene: 87199930

Centisome position: 56.76

GC content: 62.19

Gene sequence:

>1386_bases
ATGGCAATCGAACTGAAGATGCCCGCGCTCTCCCCGACGATGGAGGAGGGCACCTTGGCCAAGTGGCTCGTCAAGGCTGG
CGACGAAGTCCGGTCCGGCGACATTCTTGCAGAGATCGAGACCGACAAGGCCACGATGGAATTCGAAGCCGTGGATGAAG
GCGTGATCGCCGAAATCCTCGTCGCCGAAGGCACCGAGGGCGTGAAGGTCGGCACCGTGATCGCGACGATCCAGGGCGAG
GGCGAGGATGCCGCACCCGCAGCGGCAACTCCTGCCGTTGAACAGAAGGTCGAAATGAGCGAAGCCGCGCCAAGCGTCGA
GGCCAGGGCCGCACCCGCAGTTGCGATCGCGCCGAAGGTCGACGCAAAGCCGGCAGTAGATCCTGAGATTCCCGCCGGCA
CGGCGATGGTCCCGACCACCGTTCGCGAAGCCCTGCGCGACGCTATGGCCGAGGAAATGCGCGCCGACGACCGCGTCTTC
GTGATGGGCGAGGAAGTGGCCGAGTACCAAGGTGCCTACAAGGTTACCCAAGGCCTGCTCGATGAATTCGGTCCGCGCCG
TGTGATTGACACCCCGATCACCGAGTACGGTTTTGCGGGCATTGGCGCAGGCGCCGCGATGGGCGGCCTTCGCCCGATCA
TCGAATTCATGACGTTCAACTTCGCCATGCAGGCGATCGACCACATCATCAATTCGGCGGCCAAGACCAACTACATGTCC
GGTGGCCAGATGCGCTGCCCGATCGTGTTCCGTGGCCCCAACGGCGCTGCAAGCCGCGTCGGTGCACAGCACTCCCAGAA
CTACGGCCCGTGGTACGCCAACGTTCCCGGGCTGGTTGTCATCGCGCCATATGACAGCGCCGATGCCAAAGGCCTGATGA
AGGCTGCAATCCGCAGTGAAGACCCGGTGGTCTTCCTGGAAAACGAGCTTGTCTACGGACGCACTTTCGACGTGCCGCAG
ATGGACGACTTTGTACTGCCCATCGGCAAGGCGCGCATCGTCCGTCAGGGCAAGGACGTGACCATCGTATCATATTCGAT
CGGCGTCGGGCTTGCTCTTGAAGCGGCGGAGACTCTTGCCGCAGAGGGTATCGATGCCGAAGTGATCGATCTTCGCACGC
TGCGTCCGCTCGACAAGGACACGGTGCTGGCCTCGCTTGCCAAGACGAATCGTCTGGTTGTGGCGGAAGAAGGGTTCCCG
GTCTGCTCGATTGCTTCGGAAATCATGGCAATCTGCATGGAGGACGGGTTCGATCATCTCGACGCACCGGTCCTGCGCGT
ATGCGACGAAGACGTGCCACTGCCTTATGCCGCCAACCTCGAGAAGGCTGCGCTTATCGATGCCGGCAAGATCGCTGCGG
CCGTGCGCAAGGTCTGCTATCGCTGA

Upstream 100 bases:

>100_bases
ATTCGCCAGATCGTCGCGGAATCGGCCGACTTTGCCGAAACCTCGCCCGAGCCGGACATGGCCGAGCTCTACACTGACGT
GCTGGTGGAGAAGTACTGAG

Downstream 100 bases:

>100_bases
TCCTGCGTACCAGCCTGAATGATGAAGGGGCGTTGCAGCATTGCTGTGGCGCCCCTTGTCGTTGTCGGGCCTGGTACGCG
CGTCAACCGTGTTTACGGTT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAIELKMPALSPTMEEGTLAKWLVKAGDEVRSGDILAEIETDKATMEFEAVDEGVIAEILVAEGTEGVKVGTVIATIQGE
GEDAAPAAATPAVEQKVEMSEAAPSVEARAAPAVAIAPKVDAKPAVDPEIPAGTAMVPTTVREALRDAMAEEMRADDRVF
VMGEEVAEYQGAYKVTQGLLDEFGPRRVIDTPITEYGFAGIGAGAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMS
GGQMRCPIVFRGPNGAASRVGAQHSQNYGPWYANVPGLVVIAPYDSADAKGLMKAAIRSEDPVVFLENELVYGRTFDVPQ
MDDFVLPIGKARIVRQGKDVTIVSYSIGVGLALEAAETLAAEGIDAEVIDLRTLRPLDKDTVLASLAKTNRLVVAEEGFP
VCSIASEIMAICMEDGFDHLDAPVLRVCDEDVPLPYAANLEKAALIDAGKIAAAVRKVCYR

Sequences:

>Translated_461_residues
MAIELKMPALSPTMEEGTLAKWLVKAGDEVRSGDILAEIETDKATMEFEAVDEGVIAEILVAEGTEGVKVGTVIATIQGE
GEDAAPAAATPAVEQKVEMSEAAPSVEARAAPAVAIAPKVDAKPAVDPEIPAGTAMVPTTVREALRDAMAEEMRADDRVF
VMGEEVAEYQGAYKVTQGLLDEFGPRRVIDTPITEYGFAGIGAGAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMS
GGQMRCPIVFRGPNGAASRVGAQHSQNYGPWYANVPGLVVIAPYDSADAKGLMKAAIRSEDPVVFLENELVYGRTFDVPQ
MDDFVLPIGKARIVRQGKDVTIVSYSIGVGLALEAAETLAAEGIDAEVIDLRTLRPLDKDTVLASLAKTNRLVVAEEGFP
VCSIASEIMAICMEDGFDHLDAPVLRVCDEDVPLPYAANLEKAALIDAGKIAAAVRKVCYR
>Mature_460_residues
AIELKMPALSPTMEEGTLAKWLVKAGDEVRSGDILAEIETDKATMEFEAVDEGVIAEILVAEGTEGVKVGTVIATIQGEG
EDAAPAAATPAVEQKVEMSEAAPSVEARAAPAVAIAPKVDAKPAVDPEIPAGTAMVPTTVREALRDAMAEEMRADDRVFV
MGEEVAEYQGAYKVTQGLLDEFGPRRVIDTPITEYGFAGIGAGAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMSG
GQMRCPIVFRGPNGAASRVGAQHSQNYGPWYANVPGLVVIAPYDSADAKGLMKAAIRSEDPVVFLENELVYGRTFDVPQM
DDFVLPIGKARIVRQGKDVTIVSYSIGVGLALEAAETLAAEGIDAEVIDLRTLRPLDKDTVLASLAKTNRLVVAEEGFPV
CSIASEIMAICMEDGFDHLDAPVLRVCDEDVPLPYAANLEKAALIDAGKIAAAVRKVCYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=327, Percent_Identity=55.6574923547401, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI291084858, Length=327, Percent_Identity=52.2935779816514, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI4557353, Length=319, Percent_Identity=33.5423197492163, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI34101272, Length=319, Percent_Identity=33.5423197492163, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI203098753, Length=81, Percent_Identity=51.8518518518518, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI203098816, Length=81, Percent_Identity=51.8518518518518, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI31711992, Length=80, Percent_Identity=50, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI260898739, Length=84, Percent_Identity=45.2380952380952, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786622, Length=346, Percent_Identity=23.4104046242775, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=322, Percent_Identity=61.4906832298137, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17506935, Length=336, Percent_Identity=35.4166666666667, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=125, Percent_Identity=39.2, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319698, Length=325, Percent_Identity=57.2307692307692, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324258, Length=120, Percent_Identity=37.5, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321632, Length=109, Percent_Identity=40.3669724770642, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21358145, Length=323, Percent_Identity=59.1331269349845, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24650940, Length=323, Percent_Identity=59.1331269349845, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI160714832, Length=318, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI160714828, Length=318, Percent_Identity=33.3333333333333, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=67.4157303370787, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=67.4157303370787, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI20129315, Length=75, Percent_Identity=52, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582497, Length=64, Percent_Identity=50, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48946; Mature: 48814

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIELKMPALSPTMEEGTLAKWLVKAGDEVRSGDILAEIETDKATMEFEAVDEGVIAEIL
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEHHH
VAEGTEGVKVGTVIATIQGEGEDAAPAAATPAVEQKVEMSEAAPSVEARAAPAVAIAPKV
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCC
DAKPAVDPEIPAGTAMVPTTVREALRDAMAEEMRADDRVFVMGEEVAEYQGAYKVTQGLL
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHH
DEFGPRRVIDTPITEYGFAGIGAGAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMS
HHCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGQMRCPIVFRGPNGAASRVGAQHSQNYGPWYANVPGLVVIAPYDSADAKGLMKAAIRSE
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHCCC
DPVVFLENELVYGRTFDVPQMDDFVLPIGKARIVRQGKDVTIVSYSIGVGLALEAAETLA
CCEEEEECCEEECCCCCCCCCCCEEECCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AEGIDAEVIDLRTLRPLDKDTVLASLAKTNRLVVAEEGFPVCSIASEIMAICMEDGFDHL
HCCCCCEEEEHHHCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
DAPVLRVCDEDVPLPYAANLEKAALIDAGKIAAAVRKVCYR
CCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AIELKMPALSPTMEEGTLAKWLVKAGDEVRSGDILAEIETDKATMEFEAVDEGVIAEIL
EEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEHHCCCCCEEHHH
VAEGTEGVKVGTVIATIQGEGEDAAPAAATPAVEQKVEMSEAAPSVEARAAPAVAIAPKV
EECCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCC
DAKPAVDPEIPAGTAMVPTTVREALRDAMAEEMRADDRVFVMGEEVAEYQGAYKVTQGLL
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCHHHHHHHHH
DEFGPRRVIDTPITEYGFAGIGAGAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMS
HHCCCCCEECCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGQMRCPIVFRGPNGAASRVGAQHSQNYGPWYANVPGLVVIAPYDSADAKGLMKAAIRSE
CCCEEEEEEEECCCCHHHHHCCCCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHCCC
DPVVFLENELVYGRTFDVPQMDDFVLPIGKARIVRQGKDVTIVSYSIGVGLALEAAETLA
CCEEEEECCEEECCCCCCCCCCCEEECCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AEGIDAEVIDLRTLRPLDKDTVLASLAKTNRLVVAEEGFPVCSIASEIMAICMEDGFDHL
HCCCCCEEEEHHHCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
DAPVLRVCDEDVPLPYAANLEKAALIDAGKIAAAVRKVCYR
CCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]