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The map label for this gene is pdhA [H]

Identifier: 87199925

GI number: 87199925

Start: 2020312

End: 2021457

Strand: Direct

Name: pdhA [H]

Synonym: Saro_1908

Alternate gene names: 87199925

Gene position: 2020312-2021457 (Clockwise)

Preceding gene: 87199924

Following gene: 87199926

Centisome position: 56.73

GC content: 62.22

Gene sequence:

>1146_bases
TTGGCCAAGTCCGCCAGGCCCAGAGCCGCCAAACCCGCCGCCAAACCTGCTGCAAAATCCGCAGGCAAATCGCCCGCTTC
GGCTGCGGCCGATCCGGTGATCGCTTCCTCGGTTGCCGAAGAGGCAGCCTTCGCACTACGCAGCCTCCAGCAGGCCCATG
CGAACAACAAGCGTTACGACGCGAGCGATGCGGAACTGCTGAAGTTCTATGAGCAGATGGTCCTGATCCGCCGGTTCGAG
GAAAAGGCGGGCCAGCTTTACGGCCTCGGCCTCATCGGCGGGTTCTGCCATCTTTACATCGGCCAGGAAGCCGTGGCAGT
CGGTCTTCAGTCGGCGCTGAAGGAAGGTCACGATAGCGTGATCACCGGCTATCGCGATCACGGGCACATGCTTGCCTACG
GCATCGATCCGAAGGTGATCATGGCAGAGTTGACCGGTCGCGGCGCGGGCATCTCGCGCGGCAAGGGCGGTTCGATGCAC
ATGTTCAGCACGGACCACAAGTTCTACGGCGGTCACGGCATCGTCGGAGCGCAGGTTCCGCTCGGAGCGGGCCTTGCCTT
TGCACACAAGTATCGCGGTGACGACGGCGTGTGCATGGCTTACTTCGGCGACGGCGCGGCAAACCAGGGCCAGGTCTACG
AGACCTTCAACATGGCCGCCCTGTGGAAGCTGCCGATCATCTTCGTGGTCGAGAACAACGGCTACGCCATGGGAACCGCG
GTCAAGCGGGGGTCGGCAGAGACCGAGTTCTATCGCCGTGGCACCGCGTTCCGCATTCCAGGCATGGACGTCAACGGCAT
GGACGTTCTCGAAGTGCGCCAAGCCGCCGAGGTCGCGCTCGAGTATGTTCGTGCGGGCAACGGCCCCGTGCTCATGGAAC
TCAACACCTACCGTTACCGCGGGCATTCGATGTCCGACCCCGCAAAGTATCGCAGTCGCGAGGAAGTGCAGGAAATGCGG
GACAAGCACGATCCTATCGAAGGCGCCAAGGCAGAACTGCTGAAGCGGGGCGTGACCGAGGACAAGATCAAGGAAATCGA
CAAGCGCATTCGCCAGATCGTCGCGGAATCGGCCGACTTTGCCGAAACCTCGCCCGAGCCGGACATGGCCGAGCTCTACA
CTGACGTGCTGGTGGAGAAGTACTGA

Upstream 100 bases:

>100_bases
GACCGAACAGCGTTGCGAAGCCGTCGCGCTAGGGTATAGGCTCGGCTCGCGCGTTCCCGACGTTACGGAACCCGAGGCTG
CCAGAAAAGGAAACACAGAC

Downstream 100 bases:

>100_bases
GATGGCAATCGAACTGAAGATGCCCGCGCTCTCCCCGACGATGGAGGAGGGCACCTTGGCCAAGTGGCTCGTCAAGGCTG
GCGACGAAGTCCGGTCCGGC

Product: pyruvate dehydrogenase (lipoamide)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MAKSARPRAAKPAAKPAAKSAGKSPASAAADPVIASSVAEEAAFALRSLQQAHANNKRYDASDAELLKFYEQMVLIRRFE
EKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALKEGHDSVITGYRDHGHMLAYGIDPKVIMAELTGRGAGISRGKGGSMH
MFSTDHKFYGGHGIVGAQVPLGAGLAFAHKYRGDDGVCMAYFGDGAANQGQVYETFNMAALWKLPIIFVVENNGYAMGTA
VKRGSAETEFYRRGTAFRIPGMDVNGMDVLEVRQAAEVALEYVRAGNGPVLMELNTYRYRGHSMSDPAKYRSREEVQEMR
DKHDPIEGAKAELLKRGVTEDKIKEIDKRIRQIVAESADFAETSPEPDMAELYTDVLVEKY

Sequences:

>Translated_381_residues
MAKSARPRAAKPAAKPAAKSAGKSPASAAADPVIASSVAEEAAFALRSLQQAHANNKRYDASDAELLKFYEQMVLIRRFE
EKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALKEGHDSVITGYRDHGHMLAYGIDPKVIMAELTGRGAGISRGKGGSMH
MFSTDHKFYGGHGIVGAQVPLGAGLAFAHKYRGDDGVCMAYFGDGAANQGQVYETFNMAALWKLPIIFVVENNGYAMGTA
VKRGSAETEFYRRGTAFRIPGMDVNGMDVLEVRQAAEVALEYVRAGNGPVLMELNTYRYRGHSMSDPAKYRSREEVQEMR
DKHDPIEGAKAELLKRGVTEDKIKEIDKRIRQIVAESADFAETSPEPDMAELYTDVLVEKY
>Mature_380_residues
AKSARPRAAKPAAKPAAKSAGKSPASAAADPVIASSVAEEAAFALRSLQQAHANNKRYDASDAELLKFYEQMVLIRRFEE
KAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALKEGHDSVITGYRDHGHMLAYGIDPKVIMAELTGRGAGISRGKGGSMHM
FSTDHKFYGGHGIVGAQVPLGAGLAFAHKYRGDDGVCMAYFGDGAANQGQVYETFNMAALWKLPIIFVVENNGYAMGTAV
KRGSAETEFYRRGTAFRIPGMDVNGMDVLEVRQAAEVALEYVRAGNGPVLMELNTYRYRGHSMSDPAKYRSREEVQEMRD
KHDPIEGAKAELLKRGVTEDKIKEIDKRIRQIVAESADFAETSPEPDMAELYTDVLVEKY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=313, Percent_Identity=53.0351437699681, Blast_Score=337, Evalue=9e-93,
Organism=Homo sapiens, GI291084742, Length=308, Percent_Identity=53.8961038961039, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI4505685, Length=308, Percent_Identity=53.8961038961039, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI291084744, Length=315, Percent_Identity=52.6984126984127, Blast_Score=314, Evalue=8e-86,
Organism=Homo sapiens, GI291084757, Length=308, Percent_Identity=47.0779220779221, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI258645172, Length=322, Percent_Identity=31.6770186335404, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI11386135, Length=323, Percent_Identity=31.5789473684211, Blast_Score=129, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32564172, Length=313, Percent_Identity=53.0351437699681, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17536047, Length=313, Percent_Identity=53.0351437699681, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI86563355, Length=316, Percent_Identity=32.2784810126582, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI86563357, Length=316, Percent_Identity=32.2784810126582, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321026, Length=325, Percent_Identity=51.6923076923077, Blast_Score=340, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24639744, Length=364, Percent_Identity=49.4505494505495, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI28571106, Length=364, Percent_Identity=49.4505494505495, Blast_Score=350, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24639740, Length=360, Percent_Identity=49.4444444444444, Blast_Score=348, Evalue=3e-96,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=54.2483660130719, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24639748, Length=344, Percent_Identity=50.2906976744186, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI21355903, Length=316, Percent_Identity=29.4303797468354, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 41452; Mature: 41320

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSARPRAAKPAAKPAAKSAGKSPASAAADPVIASSVAEEAAFALRSLQQAHANNKRYD
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ASDAELLKFYEQMVLIRRFEEKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALKEGHDSV
CCHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHH
ITGYRDHGHMLAYGIDPKVIMAELTGRGAGISRGKGGSMHMFSTDHKFYGGHGIVGAQVP
EECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEECCCCEEECCCC
LGAGLAFAHKYRGDDGVCMAYFGDGAANQGQVYETFNMAALWKLPIIFVVENNGYAMGTA
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHCHHEEEECCEEEEEECCCEEEEHH
VKRGSAETEFYRRGTAFRIPGMDVNGMDVLEVRQAAEVALEYVRAGNGPVLMELNTYRYR
ECCCCCHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEC
GHSMSDPAKYRSREEVQEMRDKHDPIEGAKAELLKRGVTEDKIKEIDKRIRQIVAESADF
CCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
AETSPEPDMAELYTDVLVEKY
CCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSARPRAAKPAAKPAAKSAGKSPASAAADPVIASSVAEEAAFALRSLQQAHANNKRYD
CCCCCCCCCCCCCCHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ASDAELLKFYEQMVLIRRFEEKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALKEGHDSV
CCHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHH
ITGYRDHGHMLAYGIDPKVIMAELTGRGAGISRGKGGSMHMFSTDHKFYGGHGIVGAQVP
EECCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCEECCCCEEECCCC
LGAGLAFAHKYRGDDGVCMAYFGDGAANQGQVYETFNMAALWKLPIIFVVENNGYAMGTA
CCCCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEHHCHHEEEECCEEEEEECCCEEEEHH
VKRGSAETEFYRRGTAFRIPGMDVNGMDVLEVRQAAEVALEYVRAGNGPVLMELNTYRYR
ECCCCCHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEEC
GHSMSDPAKYRSREEVQEMRDKHDPIEGAKAELLKRGVTEDKIKEIDKRIRQIVAESADF
CCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
AETSPEPDMAELYTDVLVEKY
CCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]