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The map label for this gene is sacC [H]

Identifier: 87199901

GI number: 87199901

Start: 1989933

End: 1991255

Strand: Direct

Name: sacC [H]

Synonym: Saro_1884

Alternate gene names: 87199901

Gene position: 1989933-1991255 (Clockwise)

Preceding gene: 87199900

Following gene: 87199905

Centisome position: 55.87

GC content: 66.89

Gene sequence:

>1323_bases
ATGCGACCCCTGTACCATTACGCGCCCGCCGCCAACTGGCTGAGCGACCCCAACGGCCTCGTGTGGCAGGATGGGGAGTG
GCACCTGTTCTACCAGTACAATCCCTTCGGCGAGGACTGGGGGCACATGTCCTGGGGCCACGCGGTCAGTCGCGATCTTG
TGACTTGGCAGGAACTGCCGGTGGCTCTTGCCGAAGAAGACGGGACGATGATCTTCTCGGGATCTGCAGTCATCGATCAC
CAGGGCAGCGCGGGCTTCGGAAAGGGCGCGATGGTCGCGGTCTATACCGGCGCGCGAACCGACCGGGCGCATCAGTTCCA
GAGCATTGCCGCCAGCACGGACCGGGGGCGCACTTTCACCAAGTTCACGGGCAACCCGGTGCTCGACCTGCAAATGGCGG
ACTTCCGCGATCCGAACGTGTTCTGGCACGGGCCGTCGGGCCGGTGGATCATGTCGGTCGTGCTTTCGGAGGAGAACCGC
GCGCAATTGTACGCTTCGGTGGACTTGAGGCACTGGGATCTGCTGTCCGATATTGGGCGCGACGGTGCGCCCGGGCACTT
GTGGGAATGCCCCTGGATGGTCGAGTTGCCGGTGGAGGGCACCGACGAGACACGTTGGCTGTTCAAGGTCGATGTCCTGT
CCGGCGCGGCAGGCCAGGCATCGCCGTGGCGCGTGGGGCATTTCGACGGAAGGCGGTTCGTTCCGGAGACCGGTTGGGCG
GTGGGTGACCACGGGCCTGACTTCTATGCGGCAATCGGTTGGAACGCCGCCCCGGATGCGGAAGGACGACCCGTCTGGAT
CGGATGGGCGGGCAATCACGCCTATCAGAAGTACCTCGAGCCGCAGGGCTGGCGCGGCGCGATGACGCTGCCGCGCCGTG
TCTCGCTTCGCCGTGACGAAAGTGGCTACGCGCTGGTGCAGGAAGTGGAACCGGCCTGCCGCGCTCTGTTCGGCAAGGCC
GAGGCCACGTCGCTGTGCGATGGGTGCATCCCGCTGCCGTCAGCGGCGCTGCTGAGCTTTCCGGCCGGCAAGGACTGGTC
CTTCAGGATCGAGGACGATCATGGGCGCAGCATCGAGTGCGCGCTCTCGGGGGGGCGGCTGACCGTGCGCCGACACGACC
CGGTGACGCCGCAGCTCGAACATCGCGCGGCGATGGAAGCAGGGAAGGGCCCTGTCGAACTGTGGCTCGATGCAGGCAGC
CTTGAGGTCTTCGCGAACCGGGGCGGGGCAGTTCTGACGTTGCAGCATCGCCTCGCCGGCGAGGCCTGGGCCTTGCGCGG
CGAGGGCGCGTGCAGCGTGGCCTATCCCGCCGCGATGGCCTGA

Upstream 100 bases:

>100_bases
GCCCTTTTCCCTTCGACCATGTGGCACGGCACCGTCCCCTTCGCGGATGGCGAACGGATGACCATAGCCTTCGACATCGT
ACCCAACCTGAAAGCCTGAA

Downstream 100 bases:

>100_bases
CGGCCCAGTTCGATGGCGCCGGTGATCCCGGCGTCGTCGCCTAGGCCCGGGGTCACGATGTAGCTTTCGAGCCCATCGTC
CAGTCGCGGATGATGGACAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRPLYHYAPAANWLSDPNGLVWQDGEWHLFYQYNPFGEDWGHMSWGHAVSRDLVTWQELPVALAEEDGTMIFSGSAVIDH
QGSAGFGKGAMVAVYTGARTDRAHQFQSIAASTDRGRTFTKFTGNPVLDLQMADFRDPNVFWHGPSGRWIMSVVLSEENR
AQLYASVDLRHWDLLSDIGRDGAPGHLWECPWMVELPVEGTDETRWLFKVDVLSGAAGQASPWRVGHFDGRRFVPETGWA
VGDHGPDFYAAIGWNAAPDAEGRPVWIGWAGNHAYQKYLEPQGWRGAMTLPRRVSLRRDESGYALVQEVEPACRALFGKA
EATSLCDGCIPLPSAALLSFPAGKDWSFRIEDDHGRSIECALSGGRLTVRRHDPVTPQLEHRAAMEAGKGPVELWLDAGS
LEVFANRGGAVLTLQHRLAGEAWALRGEGACSVAYPAAMA

Sequences:

>Translated_440_residues
MRPLYHYAPAANWLSDPNGLVWQDGEWHLFYQYNPFGEDWGHMSWGHAVSRDLVTWQELPVALAEEDGTMIFSGSAVIDH
QGSAGFGKGAMVAVYTGARTDRAHQFQSIAASTDRGRTFTKFTGNPVLDLQMADFRDPNVFWHGPSGRWIMSVVLSEENR
AQLYASVDLRHWDLLSDIGRDGAPGHLWECPWMVELPVEGTDETRWLFKVDVLSGAAGQASPWRVGHFDGRRFVPETGWA
VGDHGPDFYAAIGWNAAPDAEGRPVWIGWAGNHAYQKYLEPQGWRGAMTLPRRVSLRRDESGYALVQEVEPACRALFGKA
EATSLCDGCIPLPSAALLSFPAGKDWSFRIEDDHGRSIECALSGGRLTVRRHDPVTPQLEHRAAMEAGKGPVELWLDAGS
LEVFANRGGAVLTLQHRLAGEAWALRGEGACSVAYPAAMA
>Mature_440_residues
MRPLYHYAPAANWLSDPNGLVWQDGEWHLFYQYNPFGEDWGHMSWGHAVSRDLVTWQELPVALAEEDGTMIFSGSAVIDH
QGSAGFGKGAMVAVYTGARTDRAHQFQSIAASTDRGRTFTKFTGNPVLDLQMADFRDPNVFWHGPSGRWIMSVVLSEENR
AQLYASVDLRHWDLLSDIGRDGAPGHLWECPWMVELPVEGTDETRWLFKVDVLSGAAGQASPWRVGHFDGRRFVPETGWA
VGDHGPDFYAAIGWNAAPDAEGRPVWIGWAGNHAYQKYLEPQGWRGAMTLPRRVSLRRDESGYALVQEVEPACRALFGKA
EATSLCDGCIPLPSAALLSFPAGKDWSFRIEDDHGRSIECALSGGRLTVRRHDPVTPQLEHRAAMEAGKGPVELWLDAGS
LEVFANRGGAVLTLQHRLAGEAWALRGEGACSVAYPAAMA

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=312, Percent_Identity=35.5769230769231, Blast_Score=204, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040 [H]

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.80 [H]

Molecular weight: Translated: 48509; Mature: 48509

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLYHYAPAANWLSDPNGLVWQDGEWHLFYQYNPFGEDWGHMSWGHAVSRDLVTWQELP
CCCCCCCCCCCHHCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
VALAEEDGTMIFSGSAVIDHQGSAGFGKGAMVAVYTGARTDRAHQFQSIAASTDRGRTFT
EEEECCCCCEEEECCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEE
KFTGNPVLDLQMADFRDPNVFWHGPSGRWIMSVVLSEENRAQLYASVDLRHWDLLSDIGR
EECCCEEEEEEECCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHCC
DGAPGHLWECPWMVELPVEGTDETRWLFKVDVLSGAAGQASPWRVGHFDGRRFVPETGWA
CCCCCCEECCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCEECCCCCCC
VGDHGPDFYAAIGWNAAPDAEGRPVWIGWAGNHAYQKYLEPQGWRGAMTLPRRVSLRRDE
CCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCEEEEEECC
SGYALVQEVEPACRALFGKAEATSLCDGCIPLPSAALLSFPAGKDWSFRIEDDHGRSIEC
CCCEEHHHHCHHHHHHHCCCCHHHHHCCCCCCCCHHHEECCCCCCEEEEEECCCCCEEEE
ALSGGRLTVRRHDPVTPQLEHRAAMEAGKGPVELWLDAGSLEVFANRGGAVLTLQHRLAG
EEECCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEEHHHCC
EAWALRGEGACSVAYPAAMA
CEEEECCCCCEEECCCCCCC
>Mature Secondary Structure
MRPLYHYAPAANWLSDPNGLVWQDGEWHLFYQYNPFGEDWGHMSWGHAVSRDLVTWQELP
CCCCCCCCCCCHHCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
VALAEEDGTMIFSGSAVIDHQGSAGFGKGAMVAVYTGARTDRAHQFQSIAASTDRGRTFT
EEEECCCCCEEEECCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCEEE
KFTGNPVLDLQMADFRDPNVFWHGPSGRWIMSVVLSEENRAQLYASVDLRHWDLLSDIGR
EECCCEEEEEEECCCCCCCEEEECCCCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHCC
DGAPGHLWECPWMVELPVEGTDETRWLFKVDVLSGAAGQASPWRVGHFDGRRFVPETGWA
CCCCCCEECCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEECCCCCEECCCCCCC
VGDHGPDFYAAIGWNAAPDAEGRPVWIGWAGNHAYQKYLEPQGWRGAMTLPRRVSLRRDE
CCCCCCCEEEEECCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCEEEEEECC
SGYALVQEVEPACRALFGKAEATSLCDGCIPLPSAALLSFPAGKDWSFRIEDDHGRSIEC
CCCEEHHHHCHHHHHHHCCCCHHHHHCCCCCCCCHHHEECCCCCCEEEEEECCCCCEEEE
ALSGGRLTVRRHDPVTPQLEHRAAMEAGKGPVELWLDAGSLEVFANRGGAVLTLQHRLAG
EEECCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEEHHHCC
EAWALRGEGACSVAYPAAMA
CEEEECCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666 [H]