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The map label for this gene is gcvH [H]

Identifier: 87199868

GI number: 87199868

Start: 1951046

End: 1951417

Strand: Direct

Name: gcvH [H]

Synonym: Saro_1851

Alternate gene names: 87199868

Gene position: 1951046-1951417 (Clockwise)

Preceding gene: 87199867

Following gene: 87199869

Centisome position: 54.78

GC content: 64.52

Gene sequence:

>372_bases
ATGACCCGCTATTTCACCAAGGACCACGAATGGATCGCGGTCGAGGGCAGCGAAGCGACCGTCGGCATCACCGACTACGC
ACAGAGCCAGCTCGGTGACATCACCTTCGTCGAACTGCCCGGCGAGGGCAGCGAAGTCGGCAAGGGCGACAGCGCATCGG
TGGTGGACAGCGTCAAGGCCGCGTCGGACGTGTACGCCCCGGTTTCGGGCAAGGTCACTCTCGCCAATGCCGCGCTCGAA
GAGCAGCCCGAACTGGTCAACACCGACCCTGAAGGCGAGGGCTGGCTGTGGAAGATGACCCTGGCCGATGCCGCCGAACT
CGACGGCCTGATGGACGAGGCTGCCTATCAGGCCTACGTCGCGGAGCTTTGA

Upstream 100 bases:

>100_bases
GCACCGCCCTTTCCATCGACGTGCGCGGACGCAAGCTCGCCGCCAGCGTCGTGTCCATGCCTTTCGTTCCCCACCGTTAT
CACCGCAAAGGAGCCGCCTG

Downstream 100 bases:

>100_bases
CACGATGCGTTATCTTCCCCTGACCGACGCCGACCGGAGCGCCATGCTCTCGGTCGTCGGCGCGGGTTCCGTCGACGAGC
TGTTCGCCGACGTTCCCGCC

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MTRYFTKDHEWIAVEGSEATVGITDYAQSQLGDITFVELPGEGSEVGKGDSASVVDSVKAASDVYAPVSGKVTLANAALE
EQPELVNTDPEGEGWLWKMTLADAAELDGLMDEAAYQAYVAEL

Sequences:

>Translated_123_residues
MTRYFTKDHEWIAVEGSEATVGITDYAQSQLGDITFVELPGEGSEVGKGDSASVVDSVKAASDVYAPVSGKVTLANAALE
EQPELVNTDPEGEGWLWKMTLADAAELDGLMDEAAYQAYVAEL
>Mature_122_residues
TRYFTKDHEWIAVEGSEATVGITDYAQSQLGDITFVELPGEGSEVGKGDSASVVDSVKAASDVYAPVSGKVTLANAALEE
QPELVNTDPEGEGWLWKMTLADAAELDGLMDEAAYQAYVAEL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=47.1074380165289, Blast_Score=120, Evalue=2e-28,
Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=47.1074380165289, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1789271, Length=117, Percent_Identity=48.7179487179487, Blast_Score=117, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=52.991452991453, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=49.1666666666667, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=47.008547008547, Blast_Score=104, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=47.5, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13104; Mature: 12973

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYFTKDHEWIAVEGSEATVGITDYAQSQLGDITFVELPGEGSEVGKGDSASVVDSVKA
CCCCCCCCCCEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
ASDVYAPVSGKVTLANAALEEQPELVNTDPEGEGWLWKMTLADAAELDGLMDEAAYQAYV
HHHCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AEL
HCC
>Mature Secondary Structure 
TRYFTKDHEWIAVEGSEATVGITDYAQSQLGDITFVELPGEGSEVGKGDSASVVDSVKA
CCCCCCCCCEEEEECCCEEEEEHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
ASDVYAPVSGKVTLANAALEEQPELVNTDPEGEGWLWKMTLADAAELDGLMDEAAYQAYV
HHHCCCCCCCCEEEECCCHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AEL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]