| Definition | Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome. |
|---|---|
| Accession | NC_007794 |
| Length | 3,561,584 |
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The map label for this gene is topA [H]
Identifier: 87199820
GI number: 87199820
Start: 1899884
End: 1902463
Strand: Direct
Name: topA [H]
Synonym: Saro_1803
Alternate gene names: 87199820
Gene position: 1899884-1902463 (Clockwise)
Preceding gene: 87199819
Following gene: 87199826
Centisome position: 53.34
GC content: 65.0
Gene sequence:
>2580_bases ATGCAGCTTGTCATCGTAGAATCCCCCGCCAAGGCCAAGACCATCGAGAAGTACCTCGGACCTGGCTACAAGGTGCTGGC CTCCTACGGCCACGTCCGCGATCTCCCCGTGAAGGATGGGTCCGTCCGCCCGGACGAGGATTTCGCCATGGATTGGGAGC TTTACGGGGACAAGCAGGCCCGCGTGAAGGCGATCTCCGATGCGGCCAAGGGCGCGGACCGACTGATCCTCGCGACCGAC CCTGACCGCGAGGGCGAAGCCATTTCGTGGCACGTCAGGGAACTGCTGGCGAAACGACGCGTGCTTCCGAAGGAAGTCGA ACGCGTTACTTTCAACGCCATCACCAAGCAGACCGTGACCGACGCGATGAAGAAGCCGCGTGCGCTCGACCAGGACCTGA TCGACGCGTACCTGGCTCGCCGCGCGCTGGACTATCTGTTTGGGTTCACGCTTTCGCCGGTCCTGTGGCGCAAGCTGCCC GGCGCCAAGTCGGCCGGGCGCGTCCAGTCGGTCGCCCTGCGCCTCATCGTCGAGCGCGAGCGCGAGATCGAGGCCTTCCG CGCGCAGGAATACTGGTCCGTCATCGCCCGGCTCGAACATGCCGGCACCGAGTTTGCGGCGCGGCTCGTAAAGTTCGACG GGCAGAAGCTCGACAAGCTGACGCTGGGCGATGAAGGCGCGGCGATGAGGGCCAAGGGCGTGGTCGAGGCCGCGACTTTC CGCGTCGAGGAAGTCGAGACAAAGCCCACGCGCCGCAACCCCTATCCGCCGTTCACCACATCGACCCTGCAACAGGAAGC CGCGCGCAAGCTCGGCTTCTCGGCCAGCCACACCATGCGCGTGGCGCAGACGCTCTACGAAGCGGGCGCGATCACCTACA TGCGTACCGATGGCGTGCAGATGGACCCGAGCGCCATTTCGGCCGCGCGCAAGGCCATCTCGGATCGTTACGACGGCCAC TACCTGCCCGAAAAGCCCCGCCATTACGAAACTAAGGCCAAGAACGCGCAGGAAGCCCACGAGGCGATCCGGCCCACGGA TTTCTCGAAGGACCGTTACGGGTCCGCCGACGAGGCGCGGCTCTACGATCTGATCTACAAGCGTGCGATGGCCAGCCAGA TGGCGTCGGCCAATATCGAGCGGACGACCGTTACGTTGCGCGACGGCACCGGAAAGCACGAACTGCGCGCCACTGGCCAG GTGGTGAAGTTCCCCGGCTTCCTCGCCGTCTATGAGGAAGGTCGCGACCAGAAGCAGGATGGCGACGAAGAGGATGGCAG CCTGCTTCCCGCGATGGCATCGGGCGATACCCCGGCCAAGCGCGGGGTCGATGCCACCCAGCACTTCACCCAGCCGCCGC CGCGCTATTCGGAAGCGAGCCTCGTCAAGCGGCTCGAGGAACTCGGCATCGGCCGTCCTTCGACCTACGCTGCAACCCTT CAGGTGTTGAAGGACCGCAACTACGTCAGGACCGAGAAAAACCGTTTCTTCGCAGAGGAATCCGGACGACTTCTCACAGC ATTTCTCGAGCGATTCTTCCCTCGCTACGTGGCTTACGAGTTCACGGCGGGCATGGAGGAGGAGCTGGACGACGTGTCCG GTGGACGCGCCGAATGGAAGAAGGTGCTTGCCGACTTCTGGCGCGACTTCAAGCCCAAGTCCGACGAGGTGATGGAGAAG AAGCCGAGCGAGGTTACCGCCGAGCTTGACGAGTTTCTCTCCGACTACCTCTTCCCCCCGCGCGCCGATGGCACCGACCC GCGCCTCTGCCCCAAGTGCGAAGAGGGCCGTCTGTCGCTGCGCGGCGGGCGTTACGGCGCTTTCGTGGCCTGCTCGAACT ATCCCGAATGCAAGTATACCCGCAAGTTCGCGCAGCCTGGCGGAGCCGATGGCGACGGCGGCGAGGATGGTGCGCTGGGC AAGGATCCAGAGACGGGTCTGGAAGTCGTGCGCAAGGCAGGCCGATTCGGCCCCTACATCCAGCTTGGTGACGGCAAGGA GGCCAAGCGCGCCTCTATCCCCAAGGACATTGGCGAACTCGATCTGGAATGGGCGCTCAAGCTGCTGTCACTGCCGCGTG AAGTGGGCGTGCATCCGGAAACCGGAAACCCGATCACCGCCAGCATCGGGCGTTATGGCCCCTATCTGGCGCACGACGGC AAGTATGCGCGGCTCAAGTCCACTGCCGAGGTGTTCGAGACGGGCATGAACGCGGCGGTCATGCTGCTGGCTGCTGCAGC GGCCGGGGCGGGCGCGCGCGGCAGCCGTGCCGCGGCCGAACCCCTCAAGGTCTTCGGCGCGCATCCCACTTCCGGGGGTG AGATCAAGCTGATGGCAGGGCGCTATGGCCCTTACGTCACCGACGGCACCACCAACGCCACCCTCCCGCGCGACAAGCAG CCCGAGGCGCTGACGCTGGAAGAGGCGATCACGCTCATCGACGAACGTGCCGCCAAAGGCCCGGCCAAGGGCAAGAAGAA GGCCCCTGCCAAGAAGGCTTCGGCCGCGAAAAAGCCTCCCGCCGCGAAGAAGGCTCCGGCCAAGAAGGCGGCACCGAAAA AGGCCGCTGCTGCGGAATGA
Upstream 100 bases:
>100_bases GGCCTATCGGGCAACCCCGCCCGATCCTCCGGCTTGACGAGGGCGGGGTCCGGTCCCCACCCTCGCGCACACGTACACGT AAGGACTACCTTTCATCGCC
Downstream 100 bases:
>100_bases CCCGGCGCTGCGGCCTGCCCCCGGGCGGGCCGCAGCCAGGCCGCGCGGCCCCTAGCGCCGTGGGCGGTAAAGCCAGTACA CCCGCGTCCCCAGCGTGAAC
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 859; Mature: 859
Protein sequence:
>859_residues MQLVIVESPAKAKTIEKYLGPGYKVLASYGHVRDLPVKDGSVRPDEDFAMDWELYGDKQARVKAISDAAKGADRLILATD PDREGEAISWHVRELLAKRRVLPKEVERVTFNAITKQTVTDAMKKPRALDQDLIDAYLARRALDYLFGFTLSPVLWRKLP GAKSAGRVQSVALRLIVEREREIEAFRAQEYWSVIARLEHAGTEFAARLVKFDGQKLDKLTLGDEGAAMRAKGVVEAATF RVEEVETKPTRRNPYPPFTTSTLQQEAARKLGFSASHTMRVAQTLYEAGAITYMRTDGVQMDPSAISAARKAISDRYDGH YLPEKPRHYETKAKNAQEAHEAIRPTDFSKDRYGSADEARLYDLIYKRAMASQMASANIERTTVTLRDGTGKHELRATGQ VVKFPGFLAVYEEGRDQKQDGDEEDGSLLPAMASGDTPAKRGVDATQHFTQPPPRYSEASLVKRLEELGIGRPSTYAATL QVLKDRNYVRTEKNRFFAEESGRLLTAFLERFFPRYVAYEFTAGMEEELDDVSGGRAEWKKVLADFWRDFKPKSDEVMEK KPSEVTAELDEFLSDYLFPPRADGTDPRLCPKCEEGRLSLRGGRYGAFVACSNYPECKYTRKFAQPGGADGDGGEDGALG KDPETGLEVVRKAGRFGPYIQLGDGKEAKRASIPKDIGELDLEWALKLLSLPREVGVHPETGNPITASIGRYGPYLAHDG KYARLKSTAEVFETGMNAAVMLLAAAAAGAGARGSRAAAEPLKVFGAHPTSGGEIKLMAGRYGPYVTDGTTNATLPRDKQ PEALTLEEAITLIDERAAKGPAKGKKKAPAKKASAAKKPPAAKKAPAKKAAPKKAAAAE
Sequences:
>Translated_859_residues MQLVIVESPAKAKTIEKYLGPGYKVLASYGHVRDLPVKDGSVRPDEDFAMDWELYGDKQARVKAISDAAKGADRLILATD PDREGEAISWHVRELLAKRRVLPKEVERVTFNAITKQTVTDAMKKPRALDQDLIDAYLARRALDYLFGFTLSPVLWRKLP GAKSAGRVQSVALRLIVEREREIEAFRAQEYWSVIARLEHAGTEFAARLVKFDGQKLDKLTLGDEGAAMRAKGVVEAATF RVEEVETKPTRRNPYPPFTTSTLQQEAARKLGFSASHTMRVAQTLYEAGAITYMRTDGVQMDPSAISAARKAISDRYDGH YLPEKPRHYETKAKNAQEAHEAIRPTDFSKDRYGSADEARLYDLIYKRAMASQMASANIERTTVTLRDGTGKHELRATGQ VVKFPGFLAVYEEGRDQKQDGDEEDGSLLPAMASGDTPAKRGVDATQHFTQPPPRYSEASLVKRLEELGIGRPSTYAATL QVLKDRNYVRTEKNRFFAEESGRLLTAFLERFFPRYVAYEFTAGMEEELDDVSGGRAEWKKVLADFWRDFKPKSDEVMEK KPSEVTAELDEFLSDYLFPPRADGTDPRLCPKCEEGRLSLRGGRYGAFVACSNYPECKYTRKFAQPGGADGDGGEDGALG KDPETGLEVVRKAGRFGPYIQLGDGKEAKRASIPKDIGELDLEWALKLLSLPREVGVHPETGNPITASIGRYGPYLAHDG KYARLKSTAEVFETGMNAAVMLLAAAAAGAGARGSRAAAEPLKVFGAHPTSGGEIKLMAGRYGPYVTDGTTNATLPRDKQ PEALTLEEAITLIDERAAKGPAKGKKKAPAKKASAAKKPPAAKKAPAKKAAPKKAAAAE >Mature_859_residues MQLVIVESPAKAKTIEKYLGPGYKVLASYGHVRDLPVKDGSVRPDEDFAMDWELYGDKQARVKAISDAAKGADRLILATD PDREGEAISWHVRELLAKRRVLPKEVERVTFNAITKQTVTDAMKKPRALDQDLIDAYLARRALDYLFGFTLSPVLWRKLP GAKSAGRVQSVALRLIVEREREIEAFRAQEYWSVIARLEHAGTEFAARLVKFDGQKLDKLTLGDEGAAMRAKGVVEAATF RVEEVETKPTRRNPYPPFTTSTLQQEAARKLGFSASHTMRVAQTLYEAGAITYMRTDGVQMDPSAISAARKAISDRYDGH YLPEKPRHYETKAKNAQEAHEAIRPTDFSKDRYGSADEARLYDLIYKRAMASQMASANIERTTVTLRDGTGKHELRATGQ VVKFPGFLAVYEEGRDQKQDGDEEDGSLLPAMASGDTPAKRGVDATQHFTQPPPRYSEASLVKRLEELGIGRPSTYAATL QVLKDRNYVRTEKNRFFAEESGRLLTAFLERFFPRYVAYEFTAGMEEELDDVSGGRAEWKKVLADFWRDFKPKSDEVMEK KPSEVTAELDEFLSDYLFPPRADGTDPRLCPKCEEGRLSLRGGRYGAFVACSNYPECKYTRKFAQPGGADGDGGEDGALG KDPETGLEVVRKAGRFGPYIQLGDGKEAKRASIPKDIGELDLEWALKLLSLPREVGVHPETGNPITASIGRYGPYLAHDG KYARLKSTAEVFETGMNAAVMLLAAAAAGAGARGSRAAAEPLKVFGAHPTSGGEIKLMAGRYGPYVTDGTTNATLPRDKQ PEALTLEEAITLIDERAAKGPAKGKKKAPAKKASAAKKPPAAKKAPAKKAAPKKAAAAE
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=591, Percent_Identity=25.0423011844332, Blast_Score=127, Evalue=5e-29, Organism=Homo sapiens, GI4507635, Length=648, Percent_Identity=22.6851851851852, Blast_Score=109, Evalue=9e-24, Organism=Escherichia coli, GI1787529, Length=648, Percent_Identity=41.5123456790123, Blast_Score=439, Evalue=1e-124, Organism=Escherichia coli, GI1788061, Length=625, Percent_Identity=25.12, Blast_Score=119, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17555378, Length=473, Percent_Identity=27.4841437632135, Blast_Score=124, Evalue=2e-28, Organism=Caenorhabditis elegans, GI32563869, Length=619, Percent_Identity=25.5250403877221, Blast_Score=123, Evalue=4e-28, Organism=Saccharomyces cerevisiae, GI6323263, Length=491, Percent_Identity=24.6435845213849, Blast_Score=105, Evalue=3e-23, Organism=Drosophila melanogaster, GI24585251, Length=586, Percent_Identity=26.4505119453925, Blast_Score=137, Evalue=3e-32, Organism=Drosophila melanogaster, GI24640096, Length=529, Percent_Identity=24.3856332703214, Blast_Score=101, Evalue=2e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 94438; Mature: 94438
Theoretical pI: Translated: 9.31; Mature: 9.31
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MQLVIVESPAKAKTIEKYLGPGYKVLASYGHVRDLPVKDGSVRPDEDFAMDWELYGDKQA CEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHH RVKAISDAAKGADRLILATDPDREGEAISWHVRELLAKRRVLPKEVERVTFNAITKQTVT HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH DAMKKPRALDQDLIDAYLARRALDYLFGFTLSPVLWRKLPGAKSAGRVQSVALRLIVERE HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH REIEAFRAQEYWSVIARLEHAGTEFAARLVKFDGQKLDKLTLGDEGAAMRAKGVVEAATF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEECCCCCHHHHCCHHHHHHH RVEEVETKPTRRNPYPPFTTSTLQQEAARKLGFSASHTMRVAQTLYEAGAITYMRTDGVQ HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCC MDPSAISAARKAISDRYDGHYLPEKPRHYETKAKNAQEAHEAIRPTDFSKDRYGSADEAR CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHH LYDLIYKRAMASQMASANIERTTVTLRDGTGKHELRATGQVVKFPGFLAVYEEGRDQKQD HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCEEECCCEEEEECCCCCCCCC GDEEDGSLLPAMASGDTPAKRGVDATQHFTQPPPRYSEASLVKRLEELGIGRPSTYAATL CCCCCCCEECCCCCCCCCHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH QVLKDRNYVRTEKNRFFAEESGRLLTAFLERFFPRYVAYEFTAGMEEELDDVSGGRAEWK HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHH KVLADFWRDFKPKSDEVMEKKPSEVTAELDEFLSDYLFPPRADGTDPRLCPKCEEGRLSL HHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE RGGRYGAFVACSNYPECKYTRKFAQPGGADGDGGEDGALGKDPETGLEVVRKAGRFGPYI CCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE QLGDGKEAKRASIPKDIGELDLEWALKLLSLPREVGVHPETGNPITASIGRYGPYLAHDG EECCCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCCCCCC KYARLKSTAEVFETGMNAAVMLLAAAAAGAGARGSRAAAEPLKVFGAHPTSGGEIKLMAG CHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHCHHHHHCCCCCCCCEEEEEEC RYGPYVTDGTTNATLPRDKQPEALTLEEAITLIDERAAKGPAKGKKKAPAKKASAAKKPP CCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC AAKKAPAKKAAPKKAAAAE CCCCCCCHHCCCCHHCCCC >Mature Secondary Structure MQLVIVESPAKAKTIEKYLGPGYKVLASYGHVRDLPVKDGSVRPDEDFAMDWELYGDKQA CEEEEECCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHH RVKAISDAAKGADRLILATDPDREGEAISWHVRELLAKRRVLPKEVERVTFNAITKQTVT HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH DAMKKPRALDQDLIDAYLARRALDYLFGFTLSPVLWRKLPGAKSAGRVQSVALRLIVERE HHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH REIEAFRAQEYWSVIARLEHAGTEFAARLVKFDGQKLDKLTLGDEGAAMRAKGVVEAATF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCEEEECCCCCHHHHCCHHHHHHH RVEEVETKPTRRNPYPPFTTSTLQQEAARKLGFSASHTMRVAQTLYEAGAITYMRTDGVQ HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCC MDPSAISAARKAISDRYDGHYLPEKPRHYETKAKNAQEAHEAIRPTDFSKDRYGSADEAR CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHCCCCCCCCCCCCCCHHHH LYDLIYKRAMASQMASANIERTTVTLRDGTGKHELRATGQVVKFPGFLAVYEEGRDQKQD HHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHCCCEEECCCEEEEECCCCCCCCC GDEEDGSLLPAMASGDTPAKRGVDATQHFTQPPPRYSEASLVKRLEELGIGRPSTYAATL CCCCCCCEECCCCCCCCCHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHH QVLKDRNYVRTEKNRFFAEESGRLLTAFLERFFPRYVAYEFTAGMEEELDDVSGGRAEWK HHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHH KVLADFWRDFKPKSDEVMEKKPSEVTAELDEFLSDYLFPPRADGTDPRLCPKCEEGRLSL HHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE RGGRYGAFVACSNYPECKYTRKFAQPGGADGDGGEDGALGKDPETGLEVVRKAGRFGPYI CCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE QLGDGKEAKRASIPKDIGELDLEWALKLLSLPREVGVHPETGNPITASIGRYGPYLAHDG EECCCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCEEECCCCCCCCCCCCC KYARLKSTAEVFETGMNAAVMLLAAAAAGAGARGSRAAAEPLKVFGAHPTSGGEIKLMAG CHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHCHHHHHCCCCCCCCEEEEEEC RYGPYVTDGTTNATLPRDKQPEALTLEEAITLIDERAAKGPAKGKKKAPAKKASAAKKPP CCCCEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCC AAKKAPAKKAAPKKAAAAE CCCCCCCHHCCCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA