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The map label for this gene is ispE

Identifier: 87199799

GI number: 87199799

Start: 1880119

End: 1880976

Strand: Direct

Name: ispE

Synonym: Saro_1782

Alternate gene names: 87199799

Gene position: 1880119-1880976 (Clockwise)

Preceding gene: 87199798

Following gene: 87199800

Centisome position: 52.79

GC content: 68.65

Gene sequence:

>858_bases
ATGCAGGAGGCGTCGGGGCTTGCTCCGGCGCCTCTTCCGTTTCAGGGGAGGGCCATGAGTCTGCGCGAAACCGCCTATGC
CAAGATCAACCTTGCCCTCCACGTAAGGCGGCGCAGGGATGACGGGTATCACGAGCTGGAAACGCTGTTCGCGTTCGTCG
ATGCGGGGGATGAGCTGACGGCGAAGCCGGCTCCTGCGGACTCCTTGCGAGTCTTCGGCGAGTTTGGCGGGGCGCTGGAC
GATCCGTTCGGTAACATCGTAGCCAAGGCGCTCGGGACCTTGCCGCATGGCGAGGGCTGGGCGGTGACGCTGGAGAAGCA
TCTCCCGGTCGCCGCGGGCCTCGGTGGAGGATCCGCCGATGCGGGGGCGGTCTTCCGCATGGTGGAGCGCAGCCACGGCC
TTCCGGCAGACTGGCATGCCCGCGCTGCCAGGCTGGGCGCGGATGTCCCCGCCTGTGTCGAAAGCGCGGCCTGCATCGGT
CGCGGCACGGGAACCGAACTCGAACCCATCCCTAACGACCTTGCCGGAACGCCGGTTCTTCTCGTCAATCCGCGCATCCC
GCTTGCGACCGGCCCGGTGTTCAAGGCCTGGGATGGCGTGGACCGGGGCGCGCTTGAAGGCGCCACCGCTCGCGAAGTCG
CCTTTGCCGGACGCAACGACCTCGAGGCGCCCGCGCTCTCGCTCGTGCCGGAGATCGGCGCTGTCCTCGTTACCCTGCGC
CAGACTGGCGGATGGCTCACGCGCATGTCCGGCTCGGGCGCAACCTGCTTCGCGCTCTACGACACGCCGGAGCAGCGCGA
TCTGGCGCAGGCCGCGATGCCGCCATCGTGGTGGACACTGGGGGGAGCCCTGCGTTGA

Upstream 100 bases:

>100_bases
ACCTGCGACATCAAGGACATGACCCAGAACATCGACTGGGTCACGCCCGAAGGCGGCGGTGGCCCGAACTACCCGAACAT
GTGATCGGGGCAGGACGAAA

Downstream 100 bases:

>100_bases
ACGAAGCCTGGAAGATCGTCGGCATACCCCGCTTCGGTGGCATCCTGGTCGTGTCCGATCATGCCTCCAACCACGTGCCG
GCCGACATCGACCTCGGCAT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MQEASGLAPAPLPFQGRAMSLRETAYAKINLALHVRRRRDDGYHELETLFAFVDAGDELTAKPAPADSLRVFGEFGGALD
DPFGNIVAKALGTLPHGEGWAVTLEKHLPVAAGLGGGSADAGAVFRMVERSHGLPADWHARAARLGADVPACVESAACIG
RGTGTELEPIPNDLAGTPVLLVNPRIPLATGPVFKAWDGVDRGALEGATAREVAFAGRNDLEAPALSLVPEIGAVLVTLR
QTGGWLTRMSGSGATCFALYDTPEQRDLAQAAMPPSWWTLGGALR

Sequences:

>Translated_285_residues
MQEASGLAPAPLPFQGRAMSLRETAYAKINLALHVRRRRDDGYHELETLFAFVDAGDELTAKPAPADSLRVFGEFGGALD
DPFGNIVAKALGTLPHGEGWAVTLEKHLPVAAGLGGGSADAGAVFRMVERSHGLPADWHARAARLGADVPACVESAACIG
RGTGTELEPIPNDLAGTPVLLVNPRIPLATGPVFKAWDGVDRGALEGATAREVAFAGRNDLEAPALSLVPEIGAVLVTLR
QTGGWLTRMSGSGATCFALYDTPEQRDLAQAAMPPSWWTLGGALR
>Mature_285_residues
MQEASGLAPAPLPFQGRAMSLRETAYAKINLALHVRRRRDDGYHELETLFAFVDAGDELTAKPAPADSLRVFGEFGGALD
DPFGNIVAKALGTLPHGEGWAVTLEKHLPVAAGLGGGSADAGAVFRMVERSHGLPADWHARAARLGADVPACVESAACIG
RGTGTELEPIPNDLAGTPVLLVNPRIPLATGPVFKAWDGVDRGALEGATAREVAFAGRNDLEAPALSLVPEIGAVLVTLR
QTGGWLTRMSGSGATCFALYDTPEQRDLAQAAMPPSWWTLGGALR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=267, Percent_Identity=31.8352059925094, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NOVAD (Q2G7F1)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497056.1
- ProteinModelPortal:   Q2G7F1
- SMR:   Q2G7F1
- STRING:   Q2G7F1
- GeneID:   3918341
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1782
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- PhylomeDB:   Q2G7F1
- ProtClustDB:   PRK14608
- BioCyc:   NARO279238:SARO_1782-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29832; Mature: 29832

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: ACT_SITE 28-28 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEASGLAPAPLPFQGRAMSLRETAYAKINLALHVRRRRDDGYHELETLFAFVDAGDELT
CCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
AKPAPADSLRVFGEFGGALDDPFGNIVAKALGTLPHGEGWAVTLEKHLPVAAGLGGGSAD
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCH
AGAVFRMVERSHGLPADWHARAARLGADVPACVESAACIGRGTGTELEPIPNDLAGTPVL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LVNPRIPLATGPVFKAWDGVDRGALEGATAREVAFAGRNDLEAPALSLVPEIGAVLVTLR
EECCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHEEEHH
QTGGWLTRMSGSGATCFALYDTPEQRDLAQAAMPPSWWTLGGALR
HCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQEASGLAPAPLPFQGRAMSLRETAYAKINLALHVRRRRDDGYHELETLFAFVDAGDELT
CCCCCCCCCCCCCCCCCCCHHHHHHEEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
AKPAPADSLRVFGEFGGALDDPFGNIVAKALGTLPHGEGWAVTLEKHLPVAAGLGGGSAD
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCH
AGAVFRMVERSHGLPADWHARAARLGADVPACVESAACIGRGTGTELEPIPNDLAGTPVL
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
LVNPRIPLATGPVFKAWDGVDRGALEGATAREVAFAGRNDLEAPALSLVPEIGAVLVTLR
EECCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHEEEHH
QTGGWLTRMSGSGATCFALYDTPEQRDLAQAAMPPSWWTLGGALR
HCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA