Click here to switch to the map view.

The map label for this gene is ahpF [H]

Identifier: 87199735

GI number: 87199735

Start: 1805578

End: 1807137

Strand: Direct

Name: ahpF [H]

Synonym: Saro_1718

Alternate gene names: 87199735

Gene position: 1805578-1807137 (Clockwise)

Preceding gene: 87199734

Following gene: 87199736

Centisome position: 50.7

GC content: 65.38

Gene sequence:

>1560_bases
ATGCCCGTTCTCGACGCCGCCACCACTGCCCAGCTCAAGGCCTATCTAGGCAACCTGCGCCGTCCGATCGAACTGGTCGC
AACGCTCGACGACAGCCCGAAGTCGGCCGAAATGCGCAGCCTCGTCGAGGAAATCGTCGCGCAGTCGGACAAGGTTTCGG
CTCGTTTCGACGGCACCGGCGAGCGCGTGCCGAGTTTTGCCATCCGCGCGGACGAGGGCAGGGCGCAGGCCGTGTTCGCG
GGCCTTCCGATGGGCCACGAATTCACTTCCCTGATCCTTGCGCTGCTCCATGTCGGCGGACACCCGCCCAAGGAAGACGC
CGACCTGCTCGACCAGGTGCGCGGCCTCGAAGGTCCGCTGCACTTCGAGACGTTCTTCTCGCTCACCTGCCAGAACTGCC
CTGACGTGGTGCAGGCGCTGAACATCATGGCGGCGCTCAATCCGAACGTCAGCCACGTCGCCATCGACGGCGCGCTGTTC
AAGGATGAGGTCGAGCGGCGCCAGATCATGGCCGTGCCGACCGTTTACCTCAACGGCGAACTGTTCGGTTCGGGCCGCAT
GGACCTCGCCCAGATCGTGGCGAAGCTGGACACCAACGCCTCTGCCCGCGCTGCCGAGAAGATCGCGGCAAAGGAGCCTT
TCGACGTGCTTGTCGTCGGCGGCGGTCCCGCAGGCGCGGCGGCTGCGATCTATGCCGCGCGCAAGGGCATCCGCACTGGC
GTCGTGGCCGAACGTTTCGGTGGCCAGGTGCTCGACACCATGGGCATCGAGAACTTCATCTCCATCCAGCATACCGAAGG
GCCGAAGCTGGCGGCGCAACTGGAACAGCACGTCAAGGAATACGACGTCGACGTGATGAACCTCCAGCGCGCGACCGGGC
TGGTGCCGGCGGGCGATGACGGCCTGCACGAAGTGAAGCTGGAGAACGGGGCAAGCCTCAAGGCGAAGACGCTGATCCTG
TCGACCGGCGCGCGCTGGAGGCAGATGGGCGTTCCCGGCGAGGACGAGTATCGCAACAAGGGTGTCGCCTATTGCCCGCA
CTGCGACGGACCGTTGTTCAAGGGCAAGCGGACGGCTGTGATCGGTGGCGGCAACTCTGGCGTGGAAGCCGCGATCGACC
TTGCCGGCATCGTCGCCCATGTCACGCTGATCGAGTTCGACAACCAGCTTCGCGCCGACGCCGTGCTCCAGACCAAGCTG
CGCTCGCTGCCCAACGTCACCATCGTCACGTCGGCGCTCACAACCGAAGTCCTCGGTGACGGGGCGAAGGTGACGGGCCT
CGTCTACAAGGACCGCGAGACCGGCCAGACGCACACGGTCGAACTGGAAGGCATCTTCGTGCAGATCGGCCTCGTGCCCA
ACACCGAATGGCTCAAGGGCGCGATCGATCTCAGCCCGCGCGGCGAGATCGAGGTCGATGCGCGCGGCGCGACCAGTGTG
CCCGGCGTATTCGGCGCGGGCGATGCGACGACTGTGCCGTACAAGCAGATCGTCATCGCGATGGGCGAGGGTTCGAAGGC
TGCGCTGTCCGCATTCGATTACCTTATCCGACAGGGTTGA

Upstream 100 bases:

>100_bases
GCGGCCCGCCGGTTCCACGATACAGATCACATCGCCCGTTGATTTCCCGTGCGCCGACCATCCGGTGACGCGCGGACCTG
TATGTCCAAAGGATTTGACA

Downstream 100 bases:

>100_bases
TCGCACGCTTTTCAGGTCTCAAGCGGCGGCGGGGTGCAAAGGCTCCGTCGCCGCTTTTCACATGTGGCGATTGCCTTTGC
GTCGTCAGGACGTTAGCGGT

Product: pyridine nucleotide-disulfide oxidoreductase, class-II, active site

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPVLDAATTAQLKAYLGNLRRPIELVATLDDSPKSAEMRSLVEEIVAQSDKVSARFDGTGERVPSFAIRADEGRAQAVFA
GLPMGHEFTSLILALLHVGGHPPKEDADLLDQVRGLEGPLHFETFFSLTCQNCPDVVQALNIMAALNPNVSHVAIDGALF
KDEVERRQIMAVPTVYLNGELFGSGRMDLAQIVAKLDTNASARAAEKIAAKEPFDVLVVGGGPAGAAAAIYAARKGIRTG
VVAERFGGQVLDTMGIENFISIQHTEGPKLAAQLEQHVKEYDVDVMNLQRATGLVPAGDDGLHEVKLENGASLKAKTLIL
STGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRTAVIGGGNSGVEAAIDLAGIVAHVTLIEFDNQLRADAVLQTKL
RSLPNVTIVTSALTTEVLGDGAKVTGLVYKDRETGQTHTVELEGIFVQIGLVPNTEWLKGAIDLSPRGEIEVDARGATSV
PGVFGAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRQG

Sequences:

>Translated_519_residues
MPVLDAATTAQLKAYLGNLRRPIELVATLDDSPKSAEMRSLVEEIVAQSDKVSARFDGTGERVPSFAIRADEGRAQAVFA
GLPMGHEFTSLILALLHVGGHPPKEDADLLDQVRGLEGPLHFETFFSLTCQNCPDVVQALNIMAALNPNVSHVAIDGALF
KDEVERRQIMAVPTVYLNGELFGSGRMDLAQIVAKLDTNASARAAEKIAAKEPFDVLVVGGGPAGAAAAIYAARKGIRTG
VVAERFGGQVLDTMGIENFISIQHTEGPKLAAQLEQHVKEYDVDVMNLQRATGLVPAGDDGLHEVKLENGASLKAKTLIL
STGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRTAVIGGGNSGVEAAIDLAGIVAHVTLIEFDNQLRADAVLQTKL
RSLPNVTIVTSALTTEVLGDGAKVTGLVYKDRETGQTHTVELEGIFVQIGLVPNTEWLKGAIDLSPRGEIEVDARGATSV
PGVFGAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRQG
>Mature_518_residues
PVLDAATTAQLKAYLGNLRRPIELVATLDDSPKSAEMRSLVEEIVAQSDKVSARFDGTGERVPSFAIRADEGRAQAVFAG
LPMGHEFTSLILALLHVGGHPPKEDADLLDQVRGLEGPLHFETFFSLTCQNCPDVVQALNIMAALNPNVSHVAIDGALFK
DEVERRQIMAVPTVYLNGELFGSGRMDLAQIVAKLDTNASARAAEKIAAKEPFDVLVVGGGPAGAAAAIYAARKGIRTGV
VAERFGGQVLDTMGIENFISIQHTEGPKLAAQLEQHVKEYDVDVMNLQRATGLVPAGDDGLHEVKLENGASLKAKTLILS
TGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRTAVIGGGNSGVEAAIDLAGIVAHVTLIEFDNQLRADAVLQTKLR
SLPNVTIVTSALTTEVLGDGAKVTGLVYKDRETGQTHTVELEGIFVQIGLVPNTEWLKGAIDLSPRGEIEVDARGATSVP
GVFGAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRQG

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=61.5087040618955, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=319, Percent_Identity=31.974921630094, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=345, Percent_Identity=30.7246376811594, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=315, Percent_Identity=32.0634920634921, Blast_Score=142, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55074; Mature: 54943

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLDAATTAQLKAYLGNLRRPIELVATLDDSPKSAEMRSLVEEIVAQSDKVSARFDGTG
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
ERVPSFAIRADEGRAQAVFAGLPMGHEFTSLILALLHVGGHPPKEDADLLDQVRGLEGPL
CCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
HFETFFSLTCQNCPDVVQALNIMAALNPNVSHVAIDGALFKDEVERRQIMAVPTVYLNGE
EHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHEEEECEEEECCE
LFGSGRMDLAQIVAKLDTNASARAAEKIAAKEPFDVLVVGGGPAGAAAAIYAARKGIRTG
EECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHC
VVAERFGGQVLDTMGIENFISIQHTEGPKLAAQLEQHVKEYDVDVMNLQRATGLVPAGDD
HHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCC
GLHEVKLENGASLKAKTLILSTGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRTAV
CCEEEEECCCCCCEEEEEEEECCCHHHHCCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVAHVTLIEFDNQLRADAVLQTKLRSLPNVTIVTSALTTEVLGD
EECCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCC
GAKVTGLVYKDRETGQTHTVELEGIFVQIGLVPNTEWLKGAIDLSPRGEIEVDARGATSV
CCEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCHHHHCEEECCCCCCCEEECCCCCCCC
PGVFGAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRQG
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVLDAATTAQLKAYLGNLRRPIELVATLDDSPKSAEMRSLVEEIVAQSDKVSARFDGTG
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCC
ERVPSFAIRADEGRAQAVFAGLPMGHEFTSLILALLHVGGHPPKEDADLLDQVRGLEGPL
CCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
HFETFFSLTCQNCPDVVQALNIMAALNPNVSHVAIDGALFKDEVERRQIMAVPTVYLNGE
EHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHEEEECEEEECCE
LFGSGRMDLAQIVAKLDTNASARAAEKIAAKEPFDVLVVGGGPAGAAAAIYAARKGIRTG
EECCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHC
VVAERFGGQVLDTMGIENFISIQHTEGPKLAAQLEQHVKEYDVDVMNLQRATGLVPAGDD
HHHHHHCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCC
GLHEVKLENGASLKAKTLILSTGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRTAV
CCEEEEECCCCCCEEEEEEEECCCHHHHCCCCCCHHHCCCCCEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVAHVTLIEFDNQLRADAVLQTKLRSLPNVTIVTSALTTEVLGD
EECCCCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCC
GAKVTGLVYKDRETGQTHTVELEGIFVQIGLVPNTEWLKGAIDLSPRGEIEVDARGATSV
CCEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCHHHHCEEECCCCCCCEEECCCCCCCC
PGVFGAGDATTVPYKQIVIAMGEGSKAALSAFDYLIRQG
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]