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The map label for this gene is ptxB [H]

Identifier: 87199566

GI number: 87199566

Start: 1604434

End: 1605384

Strand: Direct

Name: ptxB [H]

Synonym: Saro_1549

Alternate gene names: 87199566

Gene position: 1604434-1605384 (Clockwise)

Preceding gene: 87199565

Following gene: 87199567

Centisome position: 45.05

GC content: 63.62

Gene sequence:

>951_bases
ATGGCACGGAACGGAACCTCTCGAGACCTGTGGCGACGATTGGTCCGCTGGCTTTTCATCGGGATGGCGCTCGTCGCCGG
CTTCCACTTGCTCCAGCTCGATCCGCCCTTCGCCTCTCGTGATTTCGAGCAGCCCGTCAGGGTCGTTTTCATTCCCGCCG
ACGGTGGCACGGCAAGTGGCACCCTGGCCGACTTCAAGCCTCTTTTCGCCGCCCTCACGGAATCGACCGGAATGCGCTTC
GAACTCAACGTGACCCAATCTTACAGTGCTGCGGTCGAAGCCCTCTGCAACGGCACCGCTGATTTGGCGTTCGTCGGACC
TGCAACCTACCTACAGGCTCACGGCCGGCATTGCGCGGATCTGCTGGCGACGGGCGTGCGCGATGGGCGTGCGCAGTACT
ATTCGGGCATCTTCGTGCGCAAGGACGCGCCCTATCGCACTATTGCCGACTTGCGCGGCAGCCGCATGGCGTTCGGCGAC
ATCAATTCGACCTCGTCCTTCATAATGCCGGTGGCCATGCTGCTCGATGCGGGGATCGATCCGGCGCATGATCTGGCTGA
AGCGCGGCTGACGGGTAGCCATCCCAACAATCTTGCCGCGCTTGCTTCCGCCCGGGTCGATGCGGTGGCCATGTCGTTCG
ACTCGTTCGATCGAGCCTTGCAGGCGGGCTTGCCCGGCCTGGATTCGGTCCGTGTTCTCGCTCGCAGCGAACCGATGCCC
TATCCGCCGTTCACCGTAAGCACCCGCTTAGCGCCGGACCGCCGCCGCCGGCTGCGTGATGGTTTTGCGAAGCTGGAGAA
AGGCCGCCGGCACCTGCCTGGTTATGCGGGGAGACAACTGGATCGCTACACCGCGCACGTCAACGAAAGGGCCTTCGACG
ATCTGGCAGTCAGGCTTGCTCCCGTGACCGGCGAACGCGTCGCGGCAATTCTCCGGCGGACAGACAGATGA

Upstream 100 bases:

>100_bases
TGACCGGGAGGGCGCCGACCTGCAAAAAGGGTCGGCGCCCGACTACAGCCGGGTCACCTGACTGTCACAGGAGAGGCGTA
GCTGCGGCCGTGGCGGTGAC

Downstream 100 bases:

>100_bases
GCCGTGCAGGCCTGTCCATCAGCGTTGCCGCAATGACCCTGCGTCACGCCAACGGCACTGTCGCGCTCGATCGTGTGTCC
CTTGAACTTGGCGAAGGATC

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MARNGTSRDLWRRLVRWLFIGMALVAGFHLLQLDPPFASRDFEQPVRVVFIPADGGTASGTLADFKPLFAALTESTGMRF
ELNVTQSYSAAVEALCNGTADLAFVGPATYLQAHGRHCADLLATGVRDGRAQYYSGIFVRKDAPYRTIADLRGSRMAFGD
INSTSSFIMPVAMLLDAGIDPAHDLAEARLTGSHPNNLAALASARVDAVAMSFDSFDRALQAGLPGLDSVRVLARSEPMP
YPPFTVSTRLAPDRRRRLRDGFAKLEKGRRHLPGYAGRQLDRYTAHVNERAFDDLAVRLAPVTGERVAAILRRTDR

Sequences:

>Translated_316_residues
MARNGTSRDLWRRLVRWLFIGMALVAGFHLLQLDPPFASRDFEQPVRVVFIPADGGTASGTLADFKPLFAALTESTGMRF
ELNVTQSYSAAVEALCNGTADLAFVGPATYLQAHGRHCADLLATGVRDGRAQYYSGIFVRKDAPYRTIADLRGSRMAFGD
INSTSSFIMPVAMLLDAGIDPAHDLAEARLTGSHPNNLAALASARVDAVAMSFDSFDRALQAGLPGLDSVRVLARSEPMP
YPPFTVSTRLAPDRRRRLRDGFAKLEKGRRHLPGYAGRQLDRYTAHVNERAFDDLAVRLAPVTGERVAAILRRTDR
>Mature_315_residues
ARNGTSRDLWRRLVRWLFIGMALVAGFHLLQLDPPFASRDFEQPVRVVFIPADGGTASGTLADFKPLFAALTESTGMRFE
LNVTQSYSAAVEALCNGTADLAFVGPATYLQAHGRHCADLLATGVRDGRAQYYSGIFVRKDAPYRTIADLRGSRMAFGDI
NSTSSFIMPVAMLLDAGIDPAHDLAEARLTGSHPNNLAALASARVDAVAMSFDSFDRALQAGLPGLDSVRVLARSEPMPY
PPFTVSTRLAPDRRRRLRDGFAKLEKGRRHLPGYAGRQLDRYTAHVNERAFDDLAVRLAPVTGERVAAILRRTDR

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=250, Percent_Identity=21.6, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 34620; Mature: 34489

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNGTSRDLWRRLVRWLFIGMALVAGFHLLQLDPPFASRDFEQPVRVVFIPADGGTASG
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCC
TLADFKPLFAALTESTGMRFELNVTQSYSAAVEALCNGTADLAFVGPATYLQAHGRHCAD
CHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCHHHH
LLATGVRDGRAQYYSGIFVRKDAPYRTIADLRGSRMAFGDINSTSSFIMPVAMLLDAGID
HHHHCCCCCCHHHHCCEEEECCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
PAHDLAEARLTGSHPNNLAALASARVDAVAMSFDSFDRALQAGLPGLDSVRVLARSEPMP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
YPPFTVSTRLAPDRRRRLRDGFAKLEKGRRHLPGYAGRQLDRYTAHVNERAFDDLAVRLA
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
PVTGERVAAILRRTDR
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARNGTSRDLWRRLVRWLFIGMALVAGFHLLQLDPPFASRDFEQPVRVVFIPADGGTASG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCC
TLADFKPLFAALTESTGMRFELNVTQSYSAAVEALCNGTADLAFVGPATYLQAHGRHCAD
CHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEECHHHHHHHCCCHHHH
LLATGVRDGRAQYYSGIFVRKDAPYRTIADLRGSRMAFGDINSTSSFIMPVAMLLDAGID
HHHHCCCCCCHHHHCCEEEECCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCC
PAHDLAEARLTGSHPNNLAALASARVDAVAMSFDSFDRALQAGLPGLDSVRVLARSEPMP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
YPPFTVSTRLAPDRRRRLRDGFAKLEKGRRHLPGYAGRQLDRYTAHVNERAFDDLAVRLA
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
PVTGERVAAILRRTDR
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]