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The map label for this gene is otsA [H]

Identifier: 87199523

GI number: 87199523

Start: 1549515

End: 1550909

Strand: Direct

Name: otsA [H]

Synonym: Saro_1505

Alternate gene names: 87199523

Gene position: 1549515-1550909 (Clockwise)

Preceding gene: 87199522

Following gene: 87199524

Centisome position: 43.51

GC content: 62.01

Gene sequence:

>1395_bases
ATGAGCAGGCTGATAGTCATTTCGAACCGCGTCAGCGCCGGTGGGGGCGCGCAAGGCGGACTTGCCGTCGCGCTTTCGGC
GGCCTTGCGCGAACATGGAGGGATCTGGTTCGGCTGGTCCGGCAACGAGACCAGCGAGTTTACCGGCCACATCAACATGG
AACGCGTCGATGGCGTGACCACGGCCACGGTGGATCTGGAAGAGCAGGACATCGAGGAATACTACGATGGCTACGCCAAC
CGTACACTCTGGCCGCTGTTCCACTACCGGATCGACCTGGCCGAATACGAACGCAGCTTCGCCGATGGCTACAAGCGCGC
CAACGAACGCTTTGCCGAAACGGCACTGCCGCTGATCGAGCCGGACGATCTGGTCTGGGTCCAGGATTACCATATGTTTC
CGCTGGGCAAGGAACTGCGCGCGCGCGGGGTCGAAAACCGGATCGGCTTCTTCCTGCACATTCCCTGGCCGCCCCGCCGC
CTGCTGGCGACCTTGCCCGAGGCACGCGCGCTGACCGAAAGTCTTCTCGCCTATGACCTGATCGGCTTCCACACCGAGGA
ATGGCTGCAATCCTTTTGCGATTACGTGTCGCACGAACTGGGCGGTACGATCGGCGACGATGGCTGGCTGTCCGTGGGCG
AGCGCCGTGTGCGTCTGATCGCTTGCCCGGTCGGCATCGATGCAAAGGAATTTGCCGCCCTTGCCGCAAGCGAAAAGGCC
AAGGAAACCTACGAACGTGTCCGTGTGAGTGCCGTTGGGCGCACGATGATCGTCGGGGTTGATCGGCTGGACTATTCCAA
GGGCCTGGAAGAGCGCTTCCTCGGCTACGAGCGGTTCCTTGTCGAGCACCCGGAGGAGCGCAAGGAAGTTACCCTCTTGC
AGATCGCCCCGCCCTCGCGCGGCACGGTCGACAGCTACCAGCGCATCCGCAGCACGCTCGAAGGTTTGGCCGGACGCATA
AACGGGGCCCATGCAGGTCTTGATTGGGTTCCGATCCGATACGTGAACCAAGGCTACACGCGCGACATGCTTGCCGGGGT
CTATCGCGCCAGCAAGGTGGGGCTTGTCACCCCCTTGCGCGATGGCATGAACCTTGTCGCCAAGGAGTATGTCGCCGCGC
AGGATCCCGGTGATCCCGGCGTGCTGATCCTGTCACGCTTTGCCGGTGCGGCAGAGCAGATGCCCGAAGCGCTGCTGGTC
AATCCGCACAGCGCTGAAGAGATATCCGACGCTTTGAGCCGCGCCATCACGATGCCGGGTGAAGAACGGATCCGACGTTG
GCGAACGCTCATGAATGGCGTGGAACAGCACGACGTGACGTGGTGGTCGAAGCATTTTACCGACGCGCTTAAGGACTGCC
ATGAACACGCTTGCGGCTCGGTACACAGAAACTGA

Upstream 100 bases:

>100_bases
CGGCAACCGGCGAACTCTGGGGAAACTACCCGCAGACCTACTCACTTGTCGGCCTGATCAACTGCGCCGTCCTGCTGAGC
AAACCGTGGAATACCGTACG

Downstream 100 bases:

>100_bases
GATCCAGGCACATACTTCCAAGCCACTCAACATAGAAAGCAAGTGTCTGGCCCTGTCAAAGCAACTGCACTGCTGGCGGA
GGTGAGCTGATCCGCTAAGG

Product: alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSRLIVISNRVSAGGGAQGGLAVALSAALREHGGIWFGWSGNETSEFTGHINMERVDGVTTATVDLEEQDIEEYYDGYAN
RTLWPLFHYRIDLAEYERSFADGYKRANERFAETALPLIEPDDLVWVQDYHMFPLGKELRARGVENRIGFFLHIPWPPRR
LLATLPEARALTESLLAYDLIGFHTEEWLQSFCDYVSHELGGTIGDDGWLSVGERRVRLIACPVGIDAKEFAALAASEKA
KETYERVRVSAVGRTMIVGVDRLDYSKGLEERFLGYERFLVEHPEERKEVTLLQIAPPSRGTVDSYQRIRSTLEGLAGRI
NGAHAGLDWVPIRYVNQGYTRDMLAGVYRASKVGLVTPLRDGMNLVAKEYVAAQDPGDPGVLILSRFAGAAEQMPEALLV
NPHSAEEISDALSRAITMPGEERIRRWRTLMNGVEQHDVTWWSKHFTDALKDCHEHACGSVHRN

Sequences:

>Translated_464_residues
MSRLIVISNRVSAGGGAQGGLAVALSAALREHGGIWFGWSGNETSEFTGHINMERVDGVTTATVDLEEQDIEEYYDGYAN
RTLWPLFHYRIDLAEYERSFADGYKRANERFAETALPLIEPDDLVWVQDYHMFPLGKELRARGVENRIGFFLHIPWPPRR
LLATLPEARALTESLLAYDLIGFHTEEWLQSFCDYVSHELGGTIGDDGWLSVGERRVRLIACPVGIDAKEFAALAASEKA
KETYERVRVSAVGRTMIVGVDRLDYSKGLEERFLGYERFLVEHPEERKEVTLLQIAPPSRGTVDSYQRIRSTLEGLAGRI
NGAHAGLDWVPIRYVNQGYTRDMLAGVYRASKVGLVTPLRDGMNLVAKEYVAAQDPGDPGVLILSRFAGAAEQMPEALLV
NPHSAEEISDALSRAITMPGEERIRRWRTLMNGVEQHDVTWWSKHFTDALKDCHEHACGSVHRN
>Mature_463_residues
SRLIVISNRVSAGGGAQGGLAVALSAALREHGGIWFGWSGNETSEFTGHINMERVDGVTTATVDLEEQDIEEYYDGYANR
TLWPLFHYRIDLAEYERSFADGYKRANERFAETALPLIEPDDLVWVQDYHMFPLGKELRARGVENRIGFFLHIPWPPRRL
LATLPEARALTESLLAYDLIGFHTEEWLQSFCDYVSHELGGTIGDDGWLSVGERRVRLIACPVGIDAKEFAALAASEKAK
ETYERVRVSAVGRTMIVGVDRLDYSKGLEERFLGYERFLVEHPEERKEVTLLQIAPPSRGTVDSYQRIRSTLEGLAGRIN
GAHAGLDWVPIRYVNQGYTRDMLAGVYRASKVGLVTPLRDGMNLVAKEYVAAQDPGDPGVLILSRFAGAAEQMPEALLVN
PHSAEEISDALSRAITMPGEERIRRWRTLMNGVEQHDVTWWSKHFTDALKDCHEHACGSVHRN

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=467, Percent_Identity=44.7537473233405, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI115533558, Length=429, Percent_Identity=28.2051282051282, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI115533560, Length=429, Percent_Identity=28.2051282051282, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71987012, Length=429, Percent_Identity=28.2051282051282, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319602, Length=466, Percent_Identity=33.2618025751073, Blast_Score=279, Evalue=9e-76,
Organism=Saccharomyces cerevisiae, GI6323537, Length=456, Percent_Identity=26.5350877192982, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6323917, Length=443, Percent_Identity=24.6049661399549, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320279, Length=450, Percent_Identity=24.6666666666667, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.156378600823, Blast_Score=288, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52071; Mature: 51940

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIVISNRVSAGGGAQGGLAVALSAALREHGGIWFGWSGNETSEFTGHINMERVDGVT
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHCCEE
TATVDLEEQDIEEYYDGYANRTLWPLFHYRIDLAEYERSFADGYKRANERFAETALPLIE
EEEECCCHHHHHHHHCCCCCCEEHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDDLVWVQDYHMFPLGKELRARGVENRIGFFLHIPWPPRRLLATLPEARALTESLLAYDL
CCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
IGFHTEEWLQSFCDYVSHELGGTIGDDGWLSVGERRVRLIACPVGIDAKEFAALAASEKA
HHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
KETYERVRVSAVGRTMIVGVDRLDYSKGLEERFLGYERFLVEHPEERKEVTLLQIAPPSR
HHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GTVDSYQRIRSTLEGLAGRINGAHAGLDWVPIRYVNQGYTRDMLAGVYRASKVGLVTPLR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHEECCCCCHHHHHHHHHHHCCCCEECCCH
DGMNLVAKEYVAAQDPGDPGVLILSRFAGAAEQMPEALLVNPHSAEEISDALSRAITMPG
HHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCC
EERIRRWRTLMNGVEQHDVTWWSKHFTDALKDCHEHACGSVHRN
HHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLIVISNRVSAGGGAQGGLAVALSAALREHGGIWFGWSGNETSEFTGHINMERVDGVT
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEECCCCHHHHCCEE
TATVDLEEQDIEEYYDGYANRTLWPLFHYRIDLAEYERSFADGYKRANERFAETALPLIE
EEEECCCHHHHHHHHCCCCCCEEHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDDLVWVQDYHMFPLGKELRARGVENRIGFFLHIPWPPRRLLATLPEARALTESLLAYDL
CCCEEEEECCCCCCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHH
IGFHTEEWLQSFCDYVSHELGGTIGDDGWLSVGERRVRLIACPVGIDAKEFAALAASEKA
HHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
KETYERVRVSAVGRTMIVGVDRLDYSKGLEERFLGYERFLVEHPEERKEVTLLQIAPPSR
HHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
GTVDSYQRIRSTLEGLAGRINGAHAGLDWVPIRYVNQGYTRDMLAGVYRASKVGLVTPLR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHEECCCCCHHHHHHHHHHHCCCCEECCCH
DGMNLVAKEYVAAQDPGDPGVLILSRFAGAAEQMPEALLVNPHSAEEISDALSRAITMPG
HHHHHHHHHHHHCCCCCCCCEEHHHHHCCHHHHCCCEEEECCCCHHHHHHHHHHHHCCCC
EERIRRWRTLMNGVEQHDVTWWSKHFTDALKDCHEHACGSVHRN
HHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]