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The map label for this gene is otsB [H]

Identifier: 87199521

GI number: 87199521

Start: 1547027

End: 1547737

Strand: Direct

Name: otsB [H]

Synonym: Saro_1503

Alternate gene names: 87199521

Gene position: 1547027-1547737 (Clockwise)

Preceding gene: 87199520

Following gene: 87199522

Centisome position: 43.44

GC content: 64.14

Gene sequence:

>711_bases
GTGGTTCTGCACGAAGCCACGCTTTTCCTGGATCTCGACGGCACTCTATTCGAACTTGTCGACAACCCCGAGGATGTCCG
CGCCGATCATCGGACACAGGCCCTGATCGGGCAACTCTTGCAGCACATGGATGGCAGGGTCGCAGTGGTCAGCGGACGTT
CGCTGGCCCAGATTGACGACATGCTGGGCGATGCTGCGGGTACGCTCTGGATTTCCGGCAGCCATGGCTGCGAATACCGC
TGGGACGGTGTCATCCAAAGCCCCTCGCGCCCGGCCGCGCTTGACGACGTCGCAGCCAGTTTCCGCAATTTTGCGCAAAA
CCAGCCCGGCGTTCTGGTCGAGGAGAAGAGCCTCGGCGTTGCCCTGCATTACCGCACGGCCCCCGAAGCCGGGGCCTCTG
CCCTCGCGCTGGCGCAGGAAATGGCTGGCCTGCACGACCTCTATCTTCAGCACGGCAAGTCCATGGTGGAACTGCGCGCA
GGCAGCGAGAACAAAGGTAGCGCCATCCTCACCATGATGGCGCACCCGGGCCTTGCCGGCACGACCCCGGTCTTCGCTGG
CGACGACGTGACCGATGAACCCGGCTTCGAAGCGGTCCTCGAACTTGGCGGCCACGCGATCCTTGTCGGCGAACCCAGGC
CCACGCTTGCCACCTTCCGCCTGCGCTCGCCGGAGGAACTTCGCGAATGGCTCTGGAGAGCAACGGTATGA

Upstream 100 bases:

>100_bases
GGGTTCCAATAGGGCCCGGGCGCAGCGTCCGGCTGCGGAGGCACCTTTCAGGAGAAGCACAATCGGGGATGCCACACTTG
CGTTAGCGGCTCCGCCCGAC

Downstream 100 bases:

>100_bases
CAGCTTCCCTCGATCTCTGGCCAATCGGCAACTGCCAGGTTTCGGCGCTGGTCGATACCTCGGGCGCATTTGTCTGGGGC
TGCATTCCGCGGGTCGATGG

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVLHEATLFLDLDGTLFELVDNPEDVRADHRTQALIGQLLQHMDGRVAVVSGRSLAQIDDMLGDAAGTLWISGSHGCEYR
WDGVIQSPSRPAALDDVAASFRNFAQNQPGVLVEEKSLGVALHYRTAPEAGASALALAQEMAGLHDLYLQHGKSMVELRA
GSENKGSAILTMMAHPGLAGTTPVFAGDDVTDEPGFEAVLELGGHAILVGEPRPTLATFRLRSPEELREWLWRATV

Sequences:

>Translated_236_residues
MVLHEATLFLDLDGTLFELVDNPEDVRADHRTQALIGQLLQHMDGRVAVVSGRSLAQIDDMLGDAAGTLWISGSHGCEYR
WDGVIQSPSRPAALDDVAASFRNFAQNQPGVLVEEKSLGVALHYRTAPEAGASALALAQEMAGLHDLYLQHGKSMVELRA
GSENKGSAILTMMAHPGLAGTTPVFAGDDVTDEPGFEAVLELGGHAILVGEPRPTLATFRLRSPEELREWLWRATV
>Mature_236_residues
MVLHEATLFLDLDGTLFELVDNPEDVRADHRTQALIGQLLQHMDGRVAVVSGRSLAQIDDMLGDAAGTLWISGSHGCEYR
WDGVIQSPSRPAALDDVAASFRNFAQNQPGVLVEEKSLGVALHYRTAPEAGASALALAQEMAGLHDLYLQHGKSMVELRA
GSENKGSAILTMMAHPGLAGTTPVFAGDDVTDEPGFEAVLELGGHAILVGEPRPTLATFRLRSPEELREWLWRATV

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=226, Percent_Identity=34.5132743362832, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 25415; Mature: 25415

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLHEATLFLDLDGTLFELVDNPEDVRADHRTQALIGQLLQHMDGRVAVVSGRSLAQIDD
CEEEEEEEEEECCCHHEEHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
MLGDAAGTLWISGSHGCEYRWDGVIQSPSRPAALDDVAASFRNFAQNQPGVLVEEKSLGV
HHCCCCCEEEEECCCCCCEEECCEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCE
ALHYRTAPEAGASALALAQEMAGLHDLYLQHGKSMVELRAGSENKGSAILTMMAHPGLAG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCC
TTPVFAGDDVTDEPGFEAVLELGGHAILVGEPRPTLATFRLRSPEELREWLWRATV
CCCEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVLHEATLFLDLDGTLFELVDNPEDVRADHRTQALIGQLLQHMDGRVAVVSGRSLAQIDD
CEEEEEEEEEECCCHHEEHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
MLGDAAGTLWISGSHGCEYRWDGVIQSPSRPAALDDVAASFRNFAQNQPGVLVEEKSLGV
HHCCCCCEEEEECCCCCCEEECCEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCE
ALHYRTAPEAGASALALAQEMAGLHDLYLQHGKSMVELRAGSENKGSAILTMMAHPGLAG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCCC
TTPVFAGDDVTDEPGFEAVLELGGHAILVGEPRPTLATFRLRSPEELREWLWRATV
CCCEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]