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The map label for this gene is outK [H]

Identifier: 87199407

GI number: 87199407

Start: 1430763

End: 1431749

Strand: Direct

Name: outK [H]

Synonym: Saro_1386

Alternate gene names: 87199407

Gene position: 1430763-1431749 (Clockwise)

Preceding gene: 87199406

Following gene: 87199408

Centisome position: 40.17

GC content: 67.58

Gene sequence:

>987_bases
GTGAGCGCAGGGGACCCGAAGGAGCGCGGTGCGGCACTGCTGTCCGTGTTGCTGCTGGTTGCGGTCATGGCGGTGATCGC
GGCGGTTATGCTCGACCGGCTCAACCTTGCTGCGCGGCTTGCGGGCAACGGTCAGGCGATGACTCAGGCGCGGCTTTACG
CGACAAGCGCCGAAACGCTCGCGATGGCGCAGATCAAGGGGATGGTCGACAGCTCGCAGGAGCGGACGGTGGACCGGCAA
GGGCTGCTTGGCCGCGACTTCCCGCTGCCTCTGCCGCGCGGTACGGCGATGGCGCGGGTGGAGGACGCGGGTAACTGCTT
CAACCTGAACTCGCTGGTCGAGGCCGATGCGCAAGGCGTTCTTGAGCTGCGGCTCGTCGGCCTCAACCAGATGCGCGCGC
TCATGACCGGCCTCGCCATTCCCGAAGCGGAAAGTGCGATCATCAGCGATTCGATCGCCGACTGGATCGACACCGACAAC
CGGCCCGCCCCCAACGGTGCGGAGGACGAGACCTACCAGGGTACGGCAACGCCCTATCGCACGGCCGGGCGGCTGATTGG
CGACGTCAGCGAGATCCGCGCTGTGCGGGGAATGACGCCGCAATTCTACGAACGCCTGCGCCCCTGGCTCTGCGCGCTCC
CGGCGGCGGAGCTTTCACCGATCGACGTGAACACCCTTCGGCCCGATCAGGCGCCGCTTCTAGCCATGCTCGCGCCCAAG
GCCATCCCGGTCGATCGCGCCCGCGCCATGCTGTCCGGGCGGCCCGCGCTTGGCTGGGCAAGCGGTGACGAAGCCTTGCG
CGGTTTCGGCGGAGAGGGAGCCGGGGGCGTACCGCTCAACCAGTTGCAGGTGCGCAGCCGCTGGTTCCTGTTGACGCTGA
TGGTCACGGTCGATTCGGCGGTGGTCGAGGAGCAGGCGTTGATCGACGTCGGCGACAACCCTCCAAAAGTCGCCTTCCGT
ACCTGGGGCGACCGTTTCAGCCGTTAG

Upstream 100 bases:

>100_bases
CCCAGCTCCAGACCGAGCTTCCGGTGGCGGTCGAACTGCTCCTGCCCCAGGCCGGGGTGCCGCCGCTGCGCATCGTGTCT
CTGGTCGGGGTGAACTACCA

Downstream 100 bases:

>100_bases
TGCGCGTCCAAATCGCGCCGATCTTGCTCCTCGCGCAAAATTCCGCTTCCCTTTGCCGCGCCTTTCTGCAAAGGGGCCAG
CCCGCGCAGCAGAGACTTGC

Product: general secretion pathway protein K

Products: NA

Alternate protein names: Pectic enzymes secretion protein OUTK [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSAGDPKERGAALLSVLLLVAVMAVIAAVMLDRLNLAARLAGNGQAMTQARLYATSAETLAMAQIKGMVDSSQERTVDRQ
GLLGRDFPLPLPRGTAMARVEDAGNCFNLNSLVEADAQGVLELRLVGLNQMRALMTGLAIPEAESAIISDSIADWIDTDN
RPAPNGAEDETYQGTATPYRTAGRLIGDVSEIRAVRGMTPQFYERLRPWLCALPAAELSPIDVNTLRPDQAPLLAMLAPK
AIPVDRARAMLSGRPALGWASGDEALRGFGGEGAGGVPLNQLQVRSRWFLLTLMVTVDSAVVEEQALIDVGDNPPKVAFR
TWGDRFSR

Sequences:

>Translated_328_residues
MSAGDPKERGAALLSVLLLVAVMAVIAAVMLDRLNLAARLAGNGQAMTQARLYATSAETLAMAQIKGMVDSSQERTVDRQ
GLLGRDFPLPLPRGTAMARVEDAGNCFNLNSLVEADAQGVLELRLVGLNQMRALMTGLAIPEAESAIISDSIADWIDTDN
RPAPNGAEDETYQGTATPYRTAGRLIGDVSEIRAVRGMTPQFYERLRPWLCALPAAELSPIDVNTLRPDQAPLLAMLAPK
AIPVDRARAMLSGRPALGWASGDEALRGFGGEGAGGVPLNQLQVRSRWFLLTLMVTVDSAVVEEQALIDVGDNPPKVAFR
TWGDRFSR
>Mature_327_residues
SAGDPKERGAALLSVLLLVAVMAVIAAVMLDRLNLAARLAGNGQAMTQARLYATSAETLAMAQIKGMVDSSQERTVDRQG
LLGRDFPLPLPRGTAMARVEDAGNCFNLNSLVEADAQGVLELRLVGLNQMRALMTGLAIPEAESAIISDSIADWIDTDNR
PAPNGAEDETYQGTATPYRTAGRLIGDVSEIRAVRGMTPQFYERLRPWLCALPAAELSPIDVNTLRPDQAPLLAMLAPKA
IPVDRARAMLSGRPALGWASGDEALRGFGGEGAGGVPLNQLQVRSRWFLLTLMVTVDSAVVEEQALIDVGDNPPKVAFRT
WGDRFSR

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG3156

COG function: function code U; Type II secretory pathway, component PulK

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP K family [H]

Homologues:

Organism=Escherichia coli, GI1789729, Length=256, Percent_Identity=31.640625, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005628 [H]

Pfam domain/function: PF03934 GspK [H]

EC number: NA

Molecular weight: Translated: 35132; Mature: 35001

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGDPKERGAALLSVLLLVAVMAVIAAVMLDRLNLAARLAGNGQAMTQARLYATSAETL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
AMAQIKGMVDSSQERTVDRQGLLGRDFPLPLPRGTAMARVEDAGNCFNLNSLVEADAQGV
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCEECHHHHHHHHHCCE
LELRLVGLNQMRALMTGLAIPEAESAIISDSIADWIDTDNRPAPNGAEDETYQGTATPYR
EEHEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
TAGRLIGDVSEIRAVRGMTPQFYERLRPWLCALPAAELSPIDVNTLRPDQAPLLAMLAPK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCC
AIPVDRARAMLSGRPALGWASGDEALRGFGGEGAGGVPLNQLQVRSRWFLLTLMVTVDSA
CCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VVEEQALIDVGDNPPKVAFRTWGDRFSR
HHHHHHEEECCCCCCCCHHCCCHHHCCC
>Mature Secondary Structure 
SAGDPKERGAALLSVLLLVAVMAVIAAVMLDRLNLAARLAGNGQAMTQARLYATSAETL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHH
AMAQIKGMVDSSQERTVDRQGLLGRDFPLPLPRGTAMARVEDAGNCFNLNSLVEADAQGV
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCEECHHHHHHHHHCCE
LELRLVGLNQMRALMTGLAIPEAESAIISDSIADWIDTDNRPAPNGAEDETYQGTATPYR
EEHEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
TAGRLIGDVSEIRAVRGMTPQFYERLRPWLCALPAAELSPIDVNTLRPDQAPLLAMLAPK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCC
AIPVDRARAMLSGRPALGWASGDEALRGFGGEGAGGVPLNQLQVRSRWFLLTLMVTVDSA
CCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VVEEQALIDVGDNPPKVAFRTWGDRFSR
HHHHHHEEECCCCCCCCHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859 [H]