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The map label for this gene is gloB [H]

Identifier: 87199342

GI number: 87199342

Start: 1364792

End: 1365556

Strand: Reverse

Name: gloB [H]

Synonym: Saro_1321

Alternate gene names: 87199342

Gene position: 1365556-1364792 (Counterclockwise)

Preceding gene: 87199343

Following gene: 87199341

Centisome position: 38.34

GC content: 68.89

Gene sequence:

>765_bases
ATGACGCTAGAGATCCACCAGTTCCCGTGCCTGTCCGACAACTACGGCTTCCTCGTCCACGATTCCGACAGCGGCGAGAC
CGCGTGCATCGACACGCCCGATGCCGAAGCCTACCTGCGCGAGGCAAACGCCAGAGGCTGGCGGATCACGCAGATCTGGA
ACACGCACTGGCACCCCGACCATGCGGGCGGCAACGAGGCGATCAAGGCGGCAACCGGATGCACCGTCGTCGCGCCCGAG
GATGACGCCGGCAAGATCGCGGGCGTGGACCGCGCCGTCGCGCATGGCGATACCGTGCAGCTTGGCAGCTTCACCGCCGA
GGTGATCGACGTCGGCGGGCATACCATGGGCCACTGCGCCTACCACCTGCCGGAAGCCTCCGTCGCGTTCGTCGGCGACG
CGCTGTTCCCGCTGGGCTGCGGCCGCATGTTCGAGGGGACGCCGCCACAGTTCTGGGCCAGCCTCTCGCGCATCAAGGCC
CTGCCCCCCGGGACGACGCTCTACAGCGCTCACGAATATACCCAGTCCAACGCCCGCTTCGCGCTTCACGCCGATCCCGA
CAATGCCGCGCTGCGCGCTTATGCGGACGGGGTCGAAGCAAGGCGCGCGCGCGGCGAATGGACCGTGCCCACGACGCTTG
CCGCCGAACTCGCGGCGAACCCCTTCCTGCGCGCCGATACGCCCGGGATGGCCGCGCGCTGGGGCGGGTCTTCGCCCGTC
GACACATTTGCCGCCCTGCGCGCCGCCAAGGATACTTTCCGCTGA

Upstream 100 bases:

>100_bases
AGCAGTAAGCTTCACCGCCTTCAAGGCACCAGAAGCAAGGCCCGTCCGGAGAAATCCGGGCGGGCTTTTGCTTTGCCGGC
ACTGCGGCTAAGCTCGGGCC

Downstream 100 bases:

>100_bases
CGCCGATCCTGCTAAAGGGGTGTTGACCGGGGGAAAGCCTAATGCCGAGCGACAGGAAGATCATCCGCGTAACGTCGTTC
GACGTGGCCGAAGCGGCGGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTLEIHQFPCLSDNYGFLVHDSDSGETACIDTPDAEAYLREANARGWRITQIWNTHWHPDHAGGNEAIKAATGCTVVAPE
DDAGKIAGVDRAVAHGDTVQLGSFTAEVIDVGGHTMGHCAYHLPEASVAFVGDALFPLGCGRMFEGTPPQFWASLSRIKA
LPPGTTLYSAHEYTQSNARFALHADPDNAALRAYADGVEARRARGEWTVPTTLAAELAANPFLRADTPGMAARWGGSSPV
DTFAALRAAKDTFR

Sequences:

>Translated_254_residues
MTLEIHQFPCLSDNYGFLVHDSDSGETACIDTPDAEAYLREANARGWRITQIWNTHWHPDHAGGNEAIKAATGCTVVAPE
DDAGKIAGVDRAVAHGDTVQLGSFTAEVIDVGGHTMGHCAYHLPEASVAFVGDALFPLGCGRMFEGTPPQFWASLSRIKA
LPPGTTLYSAHEYTQSNARFALHADPDNAALRAYADGVEARRARGEWTVPTTLAAELAANPFLRADTPGMAARWGGSSPV
DTFAALRAAKDTFR
>Mature_253_residues
TLEIHQFPCLSDNYGFLVHDSDSGETACIDTPDAEAYLREANARGWRITQIWNTHWHPDHAGGNEAIKAATGCTVVAPED
DAGKIAGVDRAVAHGDTVQLGSFTAEVIDVGGHTMGHCAYHLPEASVAFVGDALFPLGCGRMFEGTPPQFWASLSRIKAL
PPGTTLYSAHEYTQSNARFALHADPDNAALRAYADGVEARRARGEWTVPTTLAAELAANPFLRADTPGMAARWGGSSPVD
TFAALRAAKDTFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=35.7692307692308, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=35.5212355212355, Blast_Score=168, Evalue=4e-42,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=32.295719844358, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI116642887, Length=228, Percent_Identity=32.8947368421053, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI21703352, Length=228, Percent_Identity=32.8947368421053, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI46361987, Length=205, Percent_Identity=32.6829268292683, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI41327741, Length=175, Percent_Identity=32, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1786406, Length=256, Percent_Identity=34.375, Blast_Score=134, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17536925, Length=252, Percent_Identity=33.7301587301587, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=26.9961977186312, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324614, Length=234, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=35, Blast_Score=166, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=35, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=35, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI221330176, Length=168, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27179; Mature: 27048

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEIHQFPCLSDNYGFLVHDSDSGETACIDTPDAEAYLREANARGWRITQIWNTHWHPD
CEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCC
HAGGNEAIKAATGCTVVAPEDDAGKIAGVDRAVAHGDTVQLGSFTAEVIDVGGHTMGHCA
CCCCCHHEEHHCCEEEECCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCCCCCEE
YHLPEASVAFVGDALFPLGCGRMFEGTPPQFWASLSRIKALPPGTTLYSAHEYTQSNARF
EECCCCCEEHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCEE
ALHADPDNAALRAYADGVEARRARGEWTVPTTLAAELAANPFLRADTPGMAARWGGSSPV
EEEECCCCCCHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCCCEEECCCCCCH
DTFAALRAAKDTFR
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TLEIHQFPCLSDNYGFLVHDSDSGETACIDTPDAEAYLREANARGWRITQIWNTHWHPD
EEEECCCCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCC
HAGGNEAIKAATGCTVVAPEDDAGKIAGVDRAVAHGDTVQLGSFTAEVIDVGGHTMGHCA
CCCCCHHEEHHCCEEEECCCCCCCCCCCCCHHHHCCCEEEECCCEEEEEECCCCCCCCEE
YHLPEASVAFVGDALFPLGCGRMFEGTPPQFWASLSRIKALPPGTTLYSAHEYTQSNARF
EECCCCCEEHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHCCCCCEE
ALHADPDNAALRAYADGVEARRARGEWTVPTTLAAELAANPFLRADTPGMAARWGGSSPV
EEEECCCCCCHHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCEECCCCCCEEECCCCCCH
DTFAALRAAKDTFR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA