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The map label for this gene is trpB

Identifier: 87199323

GI number: 87199323

Start: 1347061

End: 1348275

Strand: Reverse

Name: trpB

Synonym: Saro_1302

Alternate gene names: 87199323

Gene position: 1348275-1347061 (Counterclockwise)

Preceding gene: 87199325

Following gene: 87199322

Centisome position: 37.86

GC content: 67.82

Gene sequence:

>1215_bases
ATGACCGCCCAGACTCCCAACAGCTTCCGCAACCTGCCCGACGAGCGCGGCCACTTCGGCCAGTTCGGCGGTCGCTACGT
CGCCGAAACGCTGATGCCGCTGATCCTCGATCTCGAACGCGAGTACAACGCCGCGAAAGCCGACCCGGCGTTCAAGGCCG
AGTTCGACGACCTCCTGGAACACTATGTCGGCCGCCCGAGCCCGCTCTACTTCGCGCCGCGGCTGACCGAGGAGCTGGGC
GGAGCACAGGTCTGGTTCAAGCGCGACGAGCTGAACCACACCGGCGCGCACAAGATCAACAACTGCATCGGCCAGATCCT
GCTCGCCATGCGCATGGGCAAGACCAGGATCATCGCCGAGACCGGCGCGGGCCAGCACGGCGTGGCCACCGCCACCGTCT
GCGCGCGCTTCGGCCTGCCCTGCGTGATCTTCATGGGTGCGACCGACGTTGCCCGCCAGGCGCCCAACGTGTTCCGCATG
AAGCTGCTCGGCGCCGAAGTCGTGCCGGTCACGGCGGGCGCGGGCACGCTGAAGGACGCGATGAACGAGGCGCTGCGCGA
CTGGGTCGCCAACGTCCACAACACTTTCTACATCATCGGCACCGCCGCGGGCCCGCACCCCTATCCGGAACTGGTCCGCG
ACTTCCAGAGCGTGATCGGCAAGGAAGCGCGCGCGCAGATGCTCTCCCGCACCGGCCGCCTGCCCGACCTTCTGGTCGCG
GCGATCGGCGGCGGCTCCAACGCCATCGGCCTGTTCCACCCCTTCCTCGACGACCCGAGCGTCAGGATGCTGGGCGTGGA
GGCCGCCGGCCACGGCCTCGACAAGGAGCACGCCGCCAGCCTCGCGGGCGGACGCCCCGGCATCCTCCACGGCAACAAGA
CCTACCTGCTGCAGGACGAGGACGGCCAGATCACCGAAGGTCACTCGATCTCGGCTGGCCTCGACTATCCCGGCATCGGC
CCGGAACACGCCTGGCTGAAGGAAATCGGCCGCGTCGACTACACCTCGGTCACCGATACCGAGGCGCTCGACGCCTTCCA
GCTCCTGTGCCGCACCGAAGGCATCATCCCCGCGCTCGAACCGGCCCATGCCATCGCGGCGGTCAAGAAGGTCGCCCCGA
CCATGGGCAAGGACGAAATCATCCTCGCCAACCTATGCGGCCGTGGCGACAAGGACATCTTCTCGGTGGCCGAACATCTG
GGGGTGTCGCTCTGA

Upstream 100 bases:

>100_bases
GCGCGTGCAGCTTTCCGCAATGCAGCATGGGCCCTCTGCCCCAAAGGGCCGGCTTTTGCAAATCTGGACAAGCGGCGCGC
CCTCTGCCAAGCGCCGCAAC

Downstream 100 bases:

>100_bases
TGACCCGTCTTTCCAACGCCTTCGCCAAGGGCCGCCCCGCCCTCGTCACGTTCGTCACCGGCGGCGATCCCACGCCAGGC
GCCACGGATGCGATCCTCGA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTAQTPNSFRNLPDERGHFGQFGGRYVAETLMPLILDLEREYNAAKADPAFKAEFDDLLEHYVGRPSPLYFAPRLTEELG
GAQVWFKRDELNHTGAHKINNCIGQILLAMRMGKTRIIAETGAGQHGVATATVCARFGLPCVIFMGATDVARQAPNVFRM
KLLGAEVVPVTAGAGTLKDAMNEALRDWVANVHNTFYIIGTAAGPHPYPELVRDFQSVIGKEARAQMLSRTGRLPDLLVA
AIGGGSNAIGLFHPFLDDPSVRMLGVEAAGHGLDKEHAASLAGGRPGILHGNKTYLLQDEDGQITEGHSISAGLDYPGIG
PEHAWLKEIGRVDYTSVTDTEALDAFQLLCRTEGIIPALEPAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHL
GVSL

Sequences:

>Translated_404_residues
MTAQTPNSFRNLPDERGHFGQFGGRYVAETLMPLILDLEREYNAAKADPAFKAEFDDLLEHYVGRPSPLYFAPRLTEELG
GAQVWFKRDELNHTGAHKINNCIGQILLAMRMGKTRIIAETGAGQHGVATATVCARFGLPCVIFMGATDVARQAPNVFRM
KLLGAEVVPVTAGAGTLKDAMNEALRDWVANVHNTFYIIGTAAGPHPYPELVRDFQSVIGKEARAQMLSRTGRLPDLLVA
AIGGGSNAIGLFHPFLDDPSVRMLGVEAAGHGLDKEHAASLAGGRPGILHGNKTYLLQDEDGQITEGHSISAGLDYPGIG
PEHAWLKEIGRVDYTSVTDTEALDAFQLLCRTEGIIPALEPAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHL
GVSL
>Mature_403_residues
TAQTPNSFRNLPDERGHFGQFGGRYVAETLMPLILDLEREYNAAKADPAFKAEFDDLLEHYVGRPSPLYFAPRLTEELGG
AQVWFKRDELNHTGAHKINNCIGQILLAMRMGKTRIIAETGAGQHGVATATVCARFGLPCVIFMGATDVARQAPNVFRMK
LLGAEVVPVTAGAGTLKDAMNEALRDWVANVHNTFYIIGTAAGPHPYPELVRDFQSVIGKEARAQMLSRTGRLPDLLVAA
IGGGSNAIGLFHPFLDDPSVRMLGVEAAGHGLDKEHAASLAGGRPGILHGNKTYLLQDEDGQITEGHSISAGLDYPGIGP
EHAWLKEIGRVDYTSVTDTEALDAFQLLCRTEGIIPALEPAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHLG
VSL

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=381, Percent_Identity=55.6430446194226, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=57.5520833333333, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_NOVAD (Q2G8S7)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496580.1
- HSSP:   P0A2K1
- ProteinModelPortal:   Q2G8S7
- SMR:   Q2G8S7
- STRING:   Q2G8S7
- GeneID:   3917934
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1302
- NMPDR:   fig|48935.1.peg.1883
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q2G8S7
- ProtClustDB:   PRK04346
- BioCyc:   NARO279238:SARO_1302-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43432; Mature: 43301

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTPNSFRNLPDERGHFGQFGGRYVAETLMPLILDLEREYNAAKADPAFKAEFDDLLE
CCCCCCHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
HYVGRPSPLYFAPRLTEELGGAQVWFKRDELNHTGAHKINNCIGQILLAMRMGKTRIIAE
HHCCCCCCEEECCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGQHGVATATVCARFGLPCVIFMGATDVARQAPNVFRMKLLGAEVVPVTAGAGTLKDA
CCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHCCCEEEEECCCHHHHHH
MNEALRDWVANVHNTFYIIGTAAGPHPYPELVRDFQSVIGKEARAQMLSRTGRLPDLLVA
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHE
AIGGGSNAIGLFHPFLDDPSVRMLGVEAAGHGLDKEHAASLAGGRPGILHGNKTYLLQDE
EECCCCCCEEEECCCCCCCCEEEEEEEHHCCCCCHHHHHHHCCCCCCEEECCEEEEEECC
DGQITEGHSISAGLDYPGIGPEHAWLKEIGRVDYTSVTDTEALDAFQLLCRTEGIIPALE
CCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
PAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHLGVSL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAQTPNSFRNLPDERGHFGQFGGRYVAETLMPLILDLEREYNAAKADPAFKAEFDDLLE
CCCCCHHHHCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
HYVGRPSPLYFAPRLTEELGGAQVWFKRDELNHTGAHKINNCIGQILLAMRMGKTRIIAE
HHCCCCCCEEECCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
TGAGQHGVATATVCARFGLPCVIFMGATDVARQAPNVFRMKLLGAEVVPVTAGAGTLKDA
CCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHCCCEEEEECCCHHHHHH
MNEALRDWVANVHNTFYIIGTAAGPHPYPELVRDFQSVIGKEARAQMLSRTGRLPDLLVA
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHE
AIGGGSNAIGLFHPFLDDPSVRMLGVEAAGHGLDKEHAASLAGGRPGILHGNKTYLLQDE
EECCCCCCEEEECCCCCCCCEEEEEEEHHCCCCCHHHHHHHCCCCCCEEECCEEEEEECC
DGQITEGHSISAGLDYPGIGPEHAWLKEIGRVDYTSVTDTEALDAFQLLCRTEGIIPALE
CCCEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
PAHAIAAVKKVAPTMGKDEIILANLCGRGDKDIFSVAEHLGVSL
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA