Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 87199322

GI number: 87199322

Start: 1346261

End: 1347061

Strand: Reverse

Name: trpA

Synonym: Saro_1301

Alternate gene names: 87199322

Gene position: 1347061-1346261 (Counterclockwise)

Preceding gene: 87199323

Following gene: 87199321

Centisome position: 37.82

GC content: 69.41

Gene sequence:

>801_bases
ATGACCCGTCTTTCCAACGCCTTCGCCAAGGGCCGCCCCGCCCTCGTCACGTTCGTCACCGGCGGCGATCCCACGCCAGG
CGCCACGGATGCGATCCTCGACGCGCTCGTCGAAGGCGGCGCCGACGTGATCGAGCTGGGCATGCCCTTCACCGATCCCA
TGGCCGATGGCCCCGCGATCCAGCTCGCCAACCTTCGCAGCCTCGGCGCCGGCACGAAGACGGCCGACGTTTTCCGCATC
GCCGCCGATTTCCGCGCACGTCATCCGGAAGTGCCGCTGGTGCTGATGGGCTATGCCAATCCCATGGTCACGCGCGGACC
CGACTGGTTCGCCGCCGAATGCGTGAAGGCCGGGGTCGATGGCGTGATCTGCGTCGACATCCCGCCCGAGGAAGACCCGG
AACTCGGCCCCGCCCTGCGCGGCGCGGGCGTCTCGCTGATCCGCCTCGCCACGCCGACGACCGATGCGGCGCGGCTCCCC
GCCGTGCTCGAAGGCTCGTCCGGGTTCCTCTACTACGTCTCGGTCGCAGGCATCACCGGCATGCAGCAGGCGGCACAGGC
CTCGATCGAAGAGGCGGTTGCCCGCATCAAGCAGTCCGCCACGATCCCGGTCGCGGTCGGCTTCGGTGTGCGCACGCCCG
AACAGGCTGCCGCAATCGCCAAGGTGGCCGATGGCGTCGTCGTTGGCTCGGCACTGGTCGATCTGGTCGCCCGGCATGGC
GCCGATGCGGCAGGTCCGGTGAAGGAACTTACCGCCGCGCTTGCCGCTGCCGTCCACTCTGCCCGCAAGGAAATCGCATG
A

Upstream 100 bases:

>100_bases
CCCCGACCATGGGCAAGGACGAAATCATCCTCGCCAACCTATGCGGCCGTGGCGACAAGGACATCTTCTCGGTGGCCGAA
CATCTGGGGGTGTCGCTCTG

Downstream 100 bases:

>100_bases
GCTGGCTCAACAAGGTCCGCAACGCCCTCCCCTTCACGCCGAAGCGGGACACGCCGGACAACCTGTGGATCAAGTGCCCT
TCGTGCAGCGAGATGCTGTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTRLSNAFAKGRPALVTFVTGGDPTPGATDAILDALVEGGADVIELGMPFTDPMADGPAIQLANLRSLGAGTKTADVFRI
AADFRARHPEVPLVLMGYANPMVTRGPDWFAAECVKAGVDGVICVDIPPEEDPELGPALRGAGVSLIRLATPTTDAARLP
AVLEGSSGFLYYVSVAGITGMQQAAQASIEEAVARIKQSATIPVAVGFGVRTPEQAAAIAKVADGVVVGSALVDLVARHG
ADAAGPVKELTAALAAAVHSARKEIA

Sequences:

>Translated_266_residues
MTRLSNAFAKGRPALVTFVTGGDPTPGATDAILDALVEGGADVIELGMPFTDPMADGPAIQLANLRSLGAGTKTADVFRI
AADFRARHPEVPLVLMGYANPMVTRGPDWFAAECVKAGVDGVICVDIPPEEDPELGPALRGAGVSLIRLATPTTDAARLP
AVLEGSSGFLYYVSVAGITGMQQAAQASIEEAVARIKQSATIPVAVGFGVRTPEQAAAIAKVADGVVVGSALVDLVARHG
ADAAGPVKELTAALAAAVHSARKEIA
>Mature_265_residues
TRLSNAFAKGRPALVTFVTGGDPTPGATDAILDALVEGGADVIELGMPFTDPMADGPAIQLANLRSLGAGTKTADVFRIA
ADFRARHPEVPLVLMGYANPMVTRGPDWFAAECVKAGVDGVICVDIPPEEDPELGPALRGAGVSLIRLATPTTDAARLPA
VLEGSSGFLYYVSVAGITGMQQAAQASIEEAVARIKQSATIPVAVGFGVRTPEQAAAIAKVADGVVVGSALVDLVARHGA
DAAGPVKELTAALAAAVHSARKEIA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=244, Percent_Identity=37.2950819672131, Blast_Score=158, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321412, Length=233, Percent_Identity=42.0600858369099, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_NOVAD (Q2G8S8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496579.1
- HSSP:   P00929
- ProteinModelPortal:   Q2G8S8
- SMR:   Q2G8S8
- STRING:   Q2G8S8
- GeneID:   3917933
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1301
- NMPDR:   fig|48935.1.peg.1882
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   Q2G8S8
- ProtClustDB:   PRK13111
- BioCyc:   NARO279238:SARO_1301-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27042; Mature: 26911

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 45-45 ACT_SITE 56-56

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLSNAFAKGRPALVTFVTGGDPTPGATDAILDALVEGGADVIELGMPFTDPMADGPAI
CCCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCE
QLANLRSLGAGTKTADVFRIAADFRARHPEVPLVLMGYANPMVTRGPDWFAAECVKAGVD
EEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC
GVICVDIPPEEDPELGPALRGAGVSLIRLATPTTDAARLPAVLEGSSGFLYYVSVAGITG
CEEEEECCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHCCEEEECCCCEEEEEEECCCHH
MQQAAQASIEEAVARIKQSATIPVAVGFGVRTPEQAAAIAKVADGVVVGSALVDLVARHG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
ADAAGPVKELTAALAAAVHSARKEIA
CCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRLSNAFAKGRPALVTFVTGGDPTPGATDAILDALVEGGADVIELGMPFTDPMADGPAI
CCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCE
QLANLRSLGAGTKTADVFRIAADFRARHPEVPLVLMGYANPMVTRGPDWFAAECVKAGVD
EEHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCC
GVICVDIPPEEDPELGPALRGAGVSLIRLATPTTDAARLPAVLEGSSGFLYYVSVAGITG
CEEEEECCCCCCCCCCCHHHCCCEEEEEEECCCCCHHHCCEEEECCCCEEEEEEECCCHH
MQQAAQASIEEAVARIKQSATIPVAVGFGVRTPEQAAAIAKVADGVVVGSALVDLVARHG
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCC
ADAAGPVKELTAALAAAVHSARKEIA
CCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA