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The map label for this gene is rsmD [H]

Identifier: 87199317

GI number: 87199317

Start: 1341179

End: 1341721

Strand: Reverse

Name: rsmD [H]

Synonym: Saro_1296

Alternate gene names: 87199317

Gene position: 1341721-1341179 (Counterclockwise)

Preceding gene: 87199318

Following gene: 87199314

Centisome position: 37.67

GC content: 71.82

Gene sequence:

>543_bases
ATGAGAATCATTGCCGGAGAATGGCGCGGCCGTCCGCTGCGTGCGCCCGAGGGCGATACCACCCGCCCCACCGCCGACCG
CACCCGCGAAACCCTGTTCTCGATGCTCGCCAGCAGGCTGGGCGGGTTCGAGGGGCTGGCCGTGGCCGACCTGTTCGCCG
GGTCCGGCGCGCTGGGGCTTGAAGCCCTGTCGCGCGGGGCCGCGTCGGCCGTGTTCGTCGAGCAGGATCCCGCCGCCGTC
CGCGCGATCCGCGCCAACATCGCCGCGCTGCGCTGCGCCCCGCAGTGCGACGTGCGCGCCGCATCGGTGCTGACGCTGGG
ACCGGCGAAGCAGCCGCTCGACCTCGTCATGCTCGACCCGCCCTATGGCACCGGTGCCGGTGCTGTCGCGATCGACCGGC
TGGTCCGGCTGGGCTGGATCGGCGAGGCGACGTGGATCAGCCTCGAGACCGCCGTCAAGGAAGTGCCCGAGGTCAAGGTC
TGCACCGTCGACGCGCAGCGCAAGGTGGGCAAGGCGCTGCTTACGCTGATGCGGCTGAAGTAG

Upstream 100 bases:

>100_bases
CCAAGTACGGCCCGTTCGAACTGGCCGACCTGCCGCGCGGCGCCGCCGAGGAAATCCCGCAGGTCATGGTCGAACGCTTT
CGGAAGACGCTGAAGACAGC

Downstream 100 bases:

>100_bases
CCGCCTTCACGCCTCCCCGGGTTCGGCCCCCCGGGGGGAGAACTGTACGCCCGCCAGCGTCATCGCCCCAGCCAGGGTCA
GGAACACGCCGACATAGGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MRIIAGEWRGRPLRAPEGDTTRPTADRTRETLFSMLASRLGGFEGLAVADLFAGSGALGLEALSRGAASAVFVEQDPAAV
RAIRANIAALRCAPQCDVRAASVLTLGPAKQPLDLVMLDPPYGTGAGAVAIDRLVRLGWIGEATWISLETAVKEVPEVKV
CTVDAQRKVGKALLTLMRLK

Sequences:

>Translated_180_residues
MRIIAGEWRGRPLRAPEGDTTRPTADRTRETLFSMLASRLGGFEGLAVADLFAGSGALGLEALSRGAASAVFVEQDPAAV
RAIRANIAALRCAPQCDVRAASVLTLGPAKQPLDLVMLDPPYGTGAGAVAIDRLVRLGWIGEATWISLETAVKEVPEVKV
CTVDAQRKVGKALLTLMRLK
>Mature_180_residues
MRIIAGEWRGRPLRAPEGDTTRPTADRTRETLFSMLASRLGGFEGLAVADLFAGSGALGLEALSRGAASAVFVEQDPAAV
RAIRANIAALRCAPQCDVRAASVLTLGPAKQPLDLVMLDPPYGTGAGAVAIDRLVRLGWIGEATWISLETAVKEVPEVKV
CTVDAQRKVGKALLTLMRLK

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=154, Percent_Identity=40.2597402597403, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19033; Mature: 19033

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIAGEWRGRPLRAPEGDTTRPTADRTRETLFSMLASRLGGFEGLAVADLFAGSGALGL
CEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
EALSRGAASAVFVEQDPAAVRAIRANIAALRCAPQCDVRAASVLTLGPAKQPLDLVMLDP
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEECC
PYGTGAGAVAIDRLVRLGWIGEATWISLETAVKEVPEVKVCTVDAQRKVGKALLTLMRLK
CCCCCCHHHHHHHHHHHCCCCCHHEEHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIIAGEWRGRPLRAPEGDTTRPTADRTRETLFSMLASRLGGFEGLAVADLFAGSGALGL
CEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHH
EALSRGAASAVFVEQDPAAVRAIRANIAALRCAPQCDVRAASVLTLGPAKQPLDLVMLDP
HHHHCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEECC
PYGTGAGAVAIDRLVRLGWIGEATWISLETAVKEVPEVKVCTVDAQRKVGKALLTLMRLK
CCCCCCHHHHHHHHHHHCCCCCHHEEHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]