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The map label for this gene is caiD [H]

Identifier: 87199304

GI number: 87199304

Start: 1325623

End: 1326387

Strand: Direct

Name: caiD [H]

Synonym: Saro_1283

Alternate gene names: 87199304

Gene position: 1325623-1326387 (Clockwise)

Preceding gene: 87199300

Following gene: 87199309

Centisome position: 37.22

GC content: 66.41

Gene sequence:

>765_bases
ATGAGCGAGGCGGAAGTCCTTACCCAAGTCGATGGCAACGTGCTGGTCGTCACGATCAACCGTCCCGATGCGAAGAACGC
GATGAACAAGGCCGCGGCCGAGGGCATCGCAGCGGCGATGGACCGGCTCGATGCCGAGGCGGACCTGCGCTGCGCGATCC
TGACGGGGGCGGGCGGAACGTTCTGTTCGGGCATGGACCTCAAGGGCTTCCTGCGTGGCGAATCGCCCTCGATCCCGGGG
CGCGGGTTCGGCGGGCTTTCCGAATGGACGCCAAAGAAGCCGATCATTGCTGCGGTCGATGGCTATGCGCTTGCGGGCGG
CATGGAACTCGCGCTGTCGTGCGACCTGATCGTGGCCAGCGCTTCCTCGAAGTTCGGCATTCCCGAAGCCAAGCGCGGCC
TTGCCGCCGCCGCCGGCGGGCTGATCAAGCTGCCGCGCCAGATCCCGCCGCGCATTGCGATGGAACTGGCCCTGACCGGC
GACTTCATCACCGCCCAGCGCGCCTATGAACTCGGCTTCATCAACCAGGTTGTCGAAGGCCCGTCGCTCGACGCCGCCAA
GGCTCTCGCCGCGCGCGTGGCCGAGAACGGTCCGCTGGCCCTGATCGCCTCGAAGGGGATCATCCGCGATTCGCACGAAT
GGACCGAGGCCGAGATGTGGAAGAAGCAGCAGGCTTACATCGCTCCCGTATTCTCCTCCAGCGACGCCCGCGAAGGCGCC
GCCGCTTTCGCCGAGAAGCGCAAGCCCAACTGGCAGGGCAAGTAA

Upstream 100 bases:

>100_bases
CGCAATTTTTGGGTCCTTGGGACTGGACATCGGCGGTCCTTCCGGGGCAGACGTTTAAGTGACCGGTTAAAGCCGGGCGG
ATTCCATTTGGAGAGATGCG

Downstream 100 bases:

>100_bases
AACCGGCAGGGAGGGAGCGCTGGTATGGGCGCGCCCTCCCGCCATCACCCCTGCGGCAGCATTTCCTCGACCACCCGGAC
ATCTTCCGGGTTGTTCACTT

Product: short chain enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSEAEVLTQVDGNVLVVTINRPDAKNAMNKAAAEGIAAAMDRLDAEADLRCAILTGAGGTFCSGMDLKGFLRGESPSIPG
RGFGGLSEWTPKKPIIAAVDGYALAGGMELALSCDLIVASASSKFGIPEAKRGLAAAAGGLIKLPRQIPPRIAMELALTG
DFITAQRAYELGFINQVVEGPSLDAAKALAARVAENGPLALIASKGIIRDSHEWTEAEMWKKQQAYIAPVFSSSDAREGA
AAFAEKRKPNWQGK

Sequences:

>Translated_254_residues
MSEAEVLTQVDGNVLVVTINRPDAKNAMNKAAAEGIAAAMDRLDAEADLRCAILTGAGGTFCSGMDLKGFLRGESPSIPG
RGFGGLSEWTPKKPIIAAVDGYALAGGMELALSCDLIVASASSKFGIPEAKRGLAAAAGGLIKLPRQIPPRIAMELALTG
DFITAQRAYELGFINQVVEGPSLDAAKALAARVAENGPLALIASKGIIRDSHEWTEAEMWKKQQAYIAPVFSSSDAREGA
AAFAEKRKPNWQGK
>Mature_253_residues
SEAEVLTQVDGNVLVVTINRPDAKNAMNKAAAEGIAAAMDRLDAEADLRCAILTGAGGTFCSGMDLKGFLRGESPSIPGR
GFGGLSEWTPKKPIIAAVDGYALAGGMELALSCDLIVASASSKFGIPEAKRGLAAAAGGLIKLPRQIPPRIAMELALTGD
FITAQRAYELGFINQVVEGPSLDAAKALAARVAENGPLALIASKGIIRDSHEWTEAEMWKKQQAYIAPVFSSSDAREGAA
AFAEKRKPNWQGK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=31.7269076305221, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=27.6, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=29.2134831460674, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI68989263, Length=191, Percent_Identity=28.7958115183246, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=259, Percent_Identity=31.2741312741313, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI20127408, Length=200, Percent_Identity=32, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI25453481, Length=251, Percent_Identity=23.1075697211155, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI51538573, Length=251, Percent_Identity=23.1075697211155, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=25.3521126760563, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=40.1515151515151, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=32.3076923076923, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=34.2307692307692, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=264, Percent_Identity=31.8181818181818, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788682, Length=203, Percent_Identity=31.5270935960591, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=35.4838709677419, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=32.7868852459016, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=33.596837944664, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=33.4782608695652, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=250, Percent_Identity=28, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=233, Percent_Identity=27.4678111587983, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=24.7967479674797, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=26.0536398467433, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=241, Percent_Identity=24.4813278008299, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=36.2549800796813, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=36.2549800796813, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=34.2105263157895, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21357171, Length=259, Percent_Identity=29.7297297297297, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=27.843137254902, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24650670, Length=272, Percent_Identity=25.7352941176471, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921000, Length=199, Percent_Identity=31.6582914572864, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583077, Length=199, Percent_Identity=31.6582914572864, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583079, Length=199, Percent_Identity=31.6582914572864, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24654903, Length=245, Percent_Identity=24.0816326530612, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26583; Mature: 26452

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAEVLTQVDGNVLVVTINRPDAKNAMNKAAAEGIAAAMDRLDAEADLRCAILTGAGGT
CCCHHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FCSGMDLKGFLRGESPSIPGRGFGGLSEWTPKKPIIAAVDGYALAGGMELALSCDLIVAS
EECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCEEEEEEEEEEEEE
ASSKFGIPEAKRGLAAAAGGLIKLPRQIPPRIAMELALTGDFITAQRAYELGFINQVVEG
CCCCCCCCHHHHHHHHHCCCCEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHCC
PSLDAAKALAARVAENGPLALIASKGIIRDSHEWTEAEMWKKQQAYIAPVFSSSDAREGA
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
AAFAEKRKPNWQGK
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SEAEVLTQVDGNVLVVTINRPDAKNAMNKAAAEGIAAAMDRLDAEADLRCAILTGAGGT
CCHHHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
FCSGMDLKGFLRGESPSIPGRGFGGLSEWTPKKPIIAAVDGYALAGGMELALSCDLIVAS
EECCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEECCEEEEEEEEEEEEE
ASSKFGIPEAKRGLAAAAGGLIKLPRQIPPRIAMELALTGDFITAQRAYELGFINQVVEG
CCCCCCCCHHHHHHHHHCCCCEECCCCCCHHHEEEEEECCCHHHHHHHHHHHHHHHHHCC
PSLDAAKALAARVAENGPLALIASKGIIRDSHEWTEAEMWKKQQAYIAPVFSSSDAREGA
CCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHH
AAFAEKRKPNWQGK
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA