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The map label for this gene is gdhA [H]

Identifier: 87199293

GI number: 87199293

Start: 1311265

End: 1312620

Strand: Direct

Name: gdhA [H]

Synonym: Saro_1272

Alternate gene names: 87199293

Gene position: 1311265-1312620 (Clockwise)

Preceding gene: 87199292

Following gene: 87199296

Centisome position: 36.82

GC content: 62.46

Gene sequence:

>1356_bases
GTGGCCGTTTCCGATCATGTCGACCTGCCGACGTTCATGGAGGGCGTGAAGAAGCGCAATCCCTATCAGCCCGAATTCGT
CCAGGCTGTGCAGGAGGTTGCCGAGGACATCTTCGACTTCATCGAGGACAAGGAGGAATACCACGCCCAGCAGATCCTGC
GCCGCATCGCGGAGCCGGACCGGGTCGTGTCGTTCCGGGTGTGCTGGGAGGATGACAATGGCAACGTCCGTGTCCAGCGC
GGTTGGCGCGTGCAGAACAACAATGCCATCGGCCCCTACAAGGGCGGCATCCGCTTTCATCCCTCGGTCAATGAGTCCGT
CCTGAAGTTCCTCGCTTTCGAGCAGACCTTCAAGAACGCGCTGACGGGCTTGCCCATGGGGGGTGGCAAGGGCGGTTCGA
ACTTCAACCCCAAGGGCAAGAGCGTGCGCGAGATCATGCGCTTCTGCCAGTCGTTCATGACCGAGCTCTATCGCCACATC
GGCGCCGACGTCGACGTTCCGGCGGGCGATATCGGTGTTGGCGGGCGCGAGATCGGCTACATGTTCGGTCAGTACAAGCG
CATCACCAACCACTTCACCGGCGTGCTGACGGGCAAGGGTCTCGAATGGGGTGGCTCGCTTATCCGTACCGAGGCGACCG
GTTACGGCGCGGTCTATTTCCTCGCCAACATGCTGGCGACCAAGGGTGAGGACCTTCGTGGCAAGACGGCGGTGATCTCC
GGTTCGGGCAACGTCGCGACGCATGCCGCCGAGAAGATCGTCCAGTTGGGCGGAAAGGTTCTGACGCTGTCCGATTCCGG
CGGGTTCATCCATGACCCCGACGGCATCGACCAGGAGAAGATCGACTGGGTGAAGACCCACAAGACTCATCGTCGCGGGC
GCATCGAGGAATACGTCGATGCCTTTCCAGGAGCGTCGTTCCATGCAGGCAAGACGCCGTGGGGCGTGCCCTGCGATGTC
GCGCTGCCCTGCGCCACGCAGAACGAACTGCTGGGCGAGGATGCGCGCACGCTCATCGCCAACGGCTGCAAGGGCGTGGC
CGAAGGCGCCAACATGCCGACCGACCTCGAAGGCGTGCATGTGTTCAAGGCTGCCAGGATTCTCTACGCGCCGGGCAAGG
CATCGAACGCGGGGGGCGTCGCGGTTTCTGGCCTCGAGATGAGCCAGAACTCCGAACGTCGCTCGTGGAAGGAGCATGAA
CTGCAGCAGATGTTGAAGGACATCATGGCCGGCATCCACAAGTCCTGCCTGACGTACGGCGACCAGGGCGGCGGCTATAT
CGACTACGTGAAGGGCGCGAACATCGCCGGCTTCAAGAAGGTTGCCGATGCCATGCTGGCTTTCGGCGTGGTCTGA

Upstream 100 bases:

>100_bases
ATCCGACAAAAGCGCGACTCGGCATGGTCCGAAGTCGCGGCACGGGCCGCACCTGCCAGCAAGAGAAGCCGTCCATGGCT
TCGATCAGGGGGAGAGTACC

Downstream 100 bases:

>100_bases
CGGAACCGGCGCCGGCTGCACTTTCCTGCGGGGAAGCGGCCGGCGCTTCCCGGCTCAGTCCTCGAACTCGCAGGTCACCC
GCGCAAGGCAGCGCAGGAAC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH; NAD(P)H-utilizing glutamate dehydrogenase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MAVSDHVDLPTFMEGVKKRNPYQPEFVQAVQEVAEDIFDFIEDKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNVRVQR
GWRVQNNNAIGPYKGGIRFHPSVNESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
GADVDVPAGDIGVGGREIGYMFGQYKRITNHFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLATKGEDLRGKTAVIS
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGIDQEKIDWVKTHKTHRRGRIEEYVDAFPGASFHAGKTPWGVPCDV
ALPCATQNELLGEDARTLIANGCKGVAEGANMPTDLEGVHVFKAARILYAPGKASNAGGVAVSGLEMSQNSERRSWKEHE
LQQMLKDIMAGIHKSCLTYGDQGGGYIDYVKGANIAGFKKVADAMLAFGVV

Sequences:

>Translated_451_residues
MAVSDHVDLPTFMEGVKKRNPYQPEFVQAVQEVAEDIFDFIEDKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNVRVQR
GWRVQNNNAIGPYKGGIRFHPSVNESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
GADVDVPAGDIGVGGREIGYMFGQYKRITNHFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLATKGEDLRGKTAVIS
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGIDQEKIDWVKTHKTHRRGRIEEYVDAFPGASFHAGKTPWGVPCDV
ALPCATQNELLGEDARTLIANGCKGVAEGANMPTDLEGVHVFKAARILYAPGKASNAGGVAVSGLEMSQNSERRSWKEHE
LQQMLKDIMAGIHKSCLTYGDQGGGYIDYVKGANIAGFKKVADAMLAFGVV
>Mature_450_residues
AVSDHVDLPTFMEGVKKRNPYQPEFVQAVQEVAEDIFDFIEDKEEYHAQQILRRIAEPDRVVSFRVCWEDDNGNVRVQRG
WRVQNNNAIGPYKGGIRFHPSVNESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIG
ADVDVPAGDIGVGGREIGYMFGQYKRITNHFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLATKGEDLRGKTAVISG
SGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGIDQEKIDWVKTHKTHRRGRIEEYVDAFPGASFHAGKTPWGVPCDVA
LPCATQNELLGEDARTLIANGCKGVAEGANMPTDLEGVHVFKAARILYAPGKASNAGGVAVSGLEMSQNSERRSWKEHEL
QQMLKDIMAGIHKSCLTYGDQGGGYIDYVKGANIAGFKKVADAMLAFGVV

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=371, Percent_Identity=32.6145552560647, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI4885281, Length=412, Percent_Identity=30.5825242718447, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1788059, Length=444, Percent_Identity=56.5315315315315, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17544676, Length=362, Percent_Identity=31.767955801105, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=454, Percent_Identity=50.8810572687225, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319256, Length=456, Percent_Identity=51.0964912280702, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45549226, Length=390, Percent_Identity=29.4871794871795, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45553475, Length=390, Percent_Identity=29.4871794871795, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24649283, Length=362, Percent_Identity=30.1104972375691, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24649460, Length=295, Percent_Identity=30.8474576271186, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 49166; Mature: 49035

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSDHVDLPTFMEGVKKRNPYQPEFVQAVQEVAEDIFDFIEDKEEYHAQQILRRIAEPD
CCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
RVVSFRVCWEDDNGNVRVQRGWRVQNNNAIGPYKGGIRFHPSVNESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIGADVDVPAGDIGVGGREIGY
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNHFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLATKGEDLRGKTAVIS
HHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGIDQEKIDWVKTHKTHRRGRIEEYVD
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AFPGASFHAGKTPWGVPCDVALPCATQNELLGEDARTLIANGCKGVAEGANMPTDLEGVH
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHH
VFKAARILYAPGKASNAGGVAVSGLEMSQNSERRSWKEHELQQMLKDIMAGIHKSCLTYG
HHHHHHHEECCCCCCCCCCEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DQGGGYIDYVKGANIAGFKKVADAMLAFGVV
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVSDHVDLPTFMEGVKKRNPYQPEFVQAVQEVAEDIFDFIEDKEEYHAQQILRRIAEPD
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCC
RVVSFRVCWEDDNGNVRVQRGWRVQNNNAIGPYKGGIRFHPSVNESVLKFLAFEQTFKNA
CEEEEEEEEECCCCCEEEEECEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
LTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHIGADVDVPAGDIGVGGREIGY
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNHFTGVLTGKGLEWGGSLIRTEATGYGAVYFLANMLATKGEDLRGKTAVIS
HHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
GSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGIDQEKIDWVKTHKTHRRGRIEEYVD
CCCCHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHH
AFPGASFHAGKTPWGVPCDVALPCATQNELLGEDARTLIANGCKGVAEGANMPTDLEGVH
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHH
VFKAARILYAPGKASNAGGVAVSGLEMSQNSERRSWKEHELQQMLKDIMAGIHKSCLTYG
HHHHHHHEECCCCCCCCCCEEEECEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DQGGGYIDYVKGANIAGFKKVADAMLAFGVV
CCCCCEEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955404; 12663928 [H]