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The map label for this gene is gacA [H]

Identifier: 87199258

GI number: 87199258

Start: 1287299

End: 1287925

Strand: Reverse

Name: gacA [H]

Synonym: Saro_1236

Alternate gene names: 87199258

Gene position: 1287925-1287299 (Counterclockwise)

Preceding gene: 87199259

Following gene: 87199257

Centisome position: 36.16

GC content: 63.96

Gene sequence:

>627_bases
ATGATTCCTGTTCTGGTTGCTGACGACCATGGCTTCATCCGCGCCGGTGTCGAAGCCGTGTTGCGGGGCACGCGTTTCTC
CGTCGTGGCAACCACGGCGAGTGGCGAGGAAACGCTCGACGCGATCAAGACCCACGATCCGGCGGTGGTCCTCCTCGACA
TCAACATGCCCGGCATGAACGGTGTCCAGGCGCTGGAAGCCCTGCGCGAACGCGGCGACAAGCGTCCGGTCGTGCTGCTG
ACCGCCGAGATCAACGACCGCCAACTCATCTCCGTCATGCGCGCGGGCGTAGACGGGATCGTGTCGAAGGACGGCGCGGA
GGAAGGGCTGGTGGAAGTTCTTGAGAAAGTCCACGCGGGACAGAAAGCCATCGAACCGAACTTCCTGCAACGCGCGCTCG
ATCTCTCGCTTCGCCCGGACTCGCAAGGGCCGCTTGCCAAGCTGAACCCGCGCGAGCGCAAGATCGCCGCGCTCGTCGCG
CGGGGAATGCGCAACCGCGACATTGGCGCCGAACTCGGCATCGGCGAGGGCACCGTGAAAGTCTATCTCCACACGATGTA
CCAGAAGCTCGGCATCGACAACCGCACCGAACTGGCCCTGTTGGCGGTGAACGAACAGGAAGGGTGA

Upstream 100 bases:

>100_bases
CGATCAGCGAACGCGTCGAGGCACTGGGCGGTCGCTTCGAACTTCACAGCGACGGGGCCGGAACGCGCCTCGCCATTGCC
CTAAGATCCGGAGAACCTGC

Downstream 100 bases:

>100_bases
AATGCGCCGCCTCCGGTTCGGCGGGCAAAATCCCGGATCGAAACTGCGTCCAGGGTGTTGTCCCGTCGTCTAGACGGGAG
TTGCAATGTTCAGGAATGCT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIPVLVADDHGFIRAGVEAVLRGTRFSVVATTASGEETLDAIKTHDPAVVLLDINMPGMNGVQALEALRERGDKRPVVLL
TAEINDRQLISVMRAGVDGIVSKDGAEEGLVEVLEKVHAGQKAIEPNFLQRALDLSLRPDSQGPLAKLNPRERKIAALVA
RGMRNRDIGAELGIGEGTVKVYLHTMYQKLGIDNRTELALLAVNEQEG

Sequences:

>Translated_208_residues
MIPVLVADDHGFIRAGVEAVLRGTRFSVVATTASGEETLDAIKTHDPAVVLLDINMPGMNGVQALEALRERGDKRPVVLL
TAEINDRQLISVMRAGVDGIVSKDGAEEGLVEVLEKVHAGQKAIEPNFLQRALDLSLRPDSQGPLAKLNPRERKIAALVA
RGMRNRDIGAELGIGEGTVKVYLHTMYQKLGIDNRTELALLAVNEQEG
>Mature_208_residues
MIPVLVADDHGFIRAGVEAVLRGTRFSVVATTASGEETLDAIKTHDPAVVLLDINMPGMNGVQALEALRERGDKRPVVLL
TAEINDRQLISVMRAGVDGIVSKDGAEEGLVEVLEKVHAGQKAIEPNFLQRALDLSLRPDSQGPLAKLNPRERKIAALVA
RGMRNRDIGAELGIGEGTVKVYLHTMYQKLGIDNRTELALLAVNEQEG

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=30.2439024390244, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788521, Length=204, Percent_Identity=30.3921568627451, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=32.3671497584541, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=31, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1788712, Length=200, Percent_Identity=26.5, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=22.9591836734694, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1789937, Length=170, Percent_Identity=25.8823529411765, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22458; Mature: 22458

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVLVADDHGFIRAGVEAVLRGTRFSVVATTASGEETLDAIKTHDPAVVLLDINMPGMN
CCEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCC
GVQALEALRERGDKRPVVLLTAEINDRQLISVMRAGVDGIVSKDGAEEGLVEVLEKVHAG
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCC
QKAIEPNFLQRALDLSLRPDSQGPLAKLNPRERKIAALVARGMRNRDIGAELGIGEGTVK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VYLHTMYQKLGIDNRTELALLAVNEQEG
HHHHHHHHHHCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MIPVLVADDHGFIRAGVEAVLRGTRFSVVATTASGEETLDAIKTHDPAVVLLDINMPGMN
CCEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCC
GVQALEALRERGDKRPVVLLTAEINDRQLISVMRAGVDGIVSKDGAEEGLVEVLEKVHAG
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHCC
QKAIEPNFLQRALDLSLRPDSQGPLAKLNPRERKIAALVARGMRNRDIGAELGIGEGTVK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VYLHTMYQKLGIDNRTELALLAVNEQEG
HHHHHHHHHHCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]