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The map label for this gene is ligA [H]

Identifier: 87199255

GI number: 87199255

Start: 1285156

End: 1285884

Strand: Direct

Name: ligA [H]

Synonym: Saro_1233

Alternate gene names: 87199255

Gene position: 1285156-1285884 (Clockwise)

Preceding gene: 87199253

Following gene: 87199256

Centisome position: 36.08

GC content: 61.04

Gene sequence:

>729_bases
ATGGACGTTCGTGATAGGCCCAGAGCGCCAGCACTGCCCGCTCCACAGCCCGCCGGGCCTGGTCGACGATCGCAGCGCCG
GAAGCGCGCGCCTTCAGGTTCTGGCGATAGCGGCGTTGTCGTTCTGCGTTGCTCAGGGGCATGTCGTGAATTTCTAGCGA
TTAACCAAGGCGGCGACAGCCCGTTACGTTACTTACGCTCGCGTAAACCATTGGGGGCGGGCGTGTTTGACCTCGATCAA
GGAAAATTCGTATGTGTTGCATATGAAATGTCCGTCGGCCGCGCTTCCCTCTCGGGGATCGATTCGGGCCGGAAGGGAGA
AGCGGACATGACACCTGAAGGAAACCGCGAGGACCGGGCGGCCGTGGACAAGGCGCTCCGCCGCGCGATTCCGCTGTTCG
ATGGCGATCTCGCCACGCGCGGATACGAGCTCAACGCGATGTGTTTTTCCTTCAACGAAAAAGCCAATCGCGAGGCCTTT
CTGGCCGATGAAGAAGCCTATTGCCGCAAGTTCAACCTGACGCCGCAGCAACGCAAGGCCGTGGCCGATCGCGATGTGCT
CGCGATGCTCGATGCGGGCGGGAACGTCTATTATCTGGCCAAGCTGGCCGGCATTTTCGGCCTTGGCGTGCAGGACCTGG
GCGCATTGCAGACCGGCATGTCGGTCGCTGATTTCAAGGCCATGCTCGTGCGCTGGGCCGACAGTATTCCCAACAAGGAG
AACGCGTGA

Upstream 100 bases:

>100_bases
CATGTCTGCAGAAGATTGACTGGAGAACGTTCCAGTTCGGAGCGATATTCGTCGAGCGTGCGGCATCCGTCGATCTCGCT
CCACGGTACCCCGGTGGGCG

Downstream 100 bases:

>100_bases
TGGCCAAGGTCATTGGCGGTTATTTCACCAGCCACGTCCCGGGCATTGGCGGCGCCATCGTTCGCGGCGATCAGGAAACG
CCTTATTGGAAGCCGTTCTT

Product: extradiol ring-cleavage dioxygenase LigAB, LigA subunit

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDVRDRPRAPALPAPQPAGPGRRSQRRKRAPSGSGDSGVVVLRCSGACREFLAINQGGDSPLRYLRSRKPLGAGVFDLDQ
GKFVCVAYEMSVGRASLSGIDSGRKGEADMTPEGNREDRAAVDKALRRAIPLFDGDLATRGYELNAMCFSFNEKANREAF
LADEEAYCRKFNLTPQQRKAVADRDVLAMLDAGGNVYYLAKLAGIFGLGVQDLGALQTGMSVADFKAMLVRWADSIPNKE
NA

Sequences:

>Translated_242_residues
MDVRDRPRAPALPAPQPAGPGRRSQRRKRAPSGSGDSGVVVLRCSGACREFLAINQGGDSPLRYLRSRKPLGAGVFDLDQ
GKFVCVAYEMSVGRASLSGIDSGRKGEADMTPEGNREDRAAVDKALRRAIPLFDGDLATRGYELNAMCFSFNEKANREAF
LADEEAYCRKFNLTPQQRKAVADRDVLAMLDAGGNVYYLAKLAGIFGLGVQDLGALQTGMSVADFKAMLVRWADSIPNKE
NA
>Mature_242_residues
MDVRDRPRAPALPAPQPAGPGRRSQRRKRAPSGSGDSGVVVLRCSGACREFLAINQGGDSPLRYLRSRKPLGAGVFDLDQ
GKFVCVAYEMSVGRASLSGIDSGRKGEADMTPEGNREDRAAVDKALRRAIPLFDGDLATRGYELNAMCFSFNEKANREAF
LADEEAYCRKFNLTPQQRKAVADRDVLAMLDAGGNVYYLAKLAGIFGLGVQDLGALQTGMSVADFKAMLVRWADSIPNKE
NA

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 26192; Mature: 26192

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRDRPRAPALPAPQPAGPGRRSQRRKRAPSGSGDSGVVVLRCSGACREFLAINQGGDS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHEECCCCCH
PLRYLRSRKPLGAGVFDLDQGKFVCVAYEMSVGRASLSGIDSGRKGEADMTPEGNREDRA
HHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
AVDKALRRAIPLFDGDLATRGYELNAMCFSFNEKANREAFLADEEAYCRKFNLTPQQRKA
HHHHHHHHHCCCCCCCHHCCCEEEEEEEEECCCCCCCCEEECCCHHHHHCCCCCHHHHHH
VADRDVLAMLDAGGNVYYLAKLAGIFGLGVQDLGALQTGMSVADFKAMLVRWADSIPNKE
HHCCHHEEEEECCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
NA
CC
>Mature Secondary Structure
MDVRDRPRAPALPAPQPAGPGRRSQRRKRAPSGSGDSGVVVLRCSGACREFLAINQGGDS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHEECCCCCH
PLRYLRSRKPLGAGVFDLDQGKFVCVAYEMSVGRASLSGIDSGRKGEADMTPEGNREDRA
HHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
AVDKALRRAIPLFDGDLATRGYELNAMCFSFNEKANREAFLADEEAYCRKFNLTPQQRKA
HHHHHHHHHCCCCCCCHHCCCEEEEEEEEECCCCCCCCEEECCCHHHHHCCCCCHHHHHH
VADRDVLAMLDAGGNVYYLAKLAGIFGLGVQDLGALQTGMSVADFKAMLVRWADSIPNKE
HHCCHHEEEEECCCCEEEHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]