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The map label for this gene is folE [H]

Identifier: 87199252

GI number: 87199252

Start: 1282400

End: 1283035

Strand: Reverse

Name: folE [H]

Synonym: Saro_1230

Alternate gene names: 87199252

Gene position: 1283035-1282400 (Counterclockwise)

Preceding gene: 87199254

Following gene: 87199251

Centisome position: 36.02

GC content: 67.3

Gene sequence:

>636_bases
ATGAACGTGCTCTCCCCATCCCTCGCCGCGTCGTCCGGCATGGCCGACGTGATCGGCGAGGACGGCAAGATCGTGGTGCC
GGCCGACGTCGAGGAGGCGATCCGCACCCTCATCCGCTGGTCAGGCGACCAGCCCGAGCGCGAAGGCCTGCTCGACACGC
CCAAGCGCGTGGCGCGGGCCTGGCGGGAATATTGCAGCGGCTATGCTCAGGACCCGGGGCTTCACCTGTCCCGCACGTTC
GAGGAAGTCGGCGGCTACGATGAAGTCGTGCTGTTGCGCGACATCCCCTTTCAGAGCCACTGCGAACACCACATGGCGCC
GATCACCGGGACCGCATCGATCGCCTACCTGCCCCGCGACCGGGTGGTCGGCATCTCGAAGCTCGCCCGCGTACTGCACG
GATTTGCGCAGCGGCTGCAGGTGCAGGAACGGCTGACCGCGGAAGTGGCGCAATGCATCTGGGACAATCTCAAGCCGCAC
GGCGTCGCGGTGGTGATCGAGGCGCAGCATGGCTGCATGACCGGGCGCGGCGTCAGGACGCACGGCGTGGGCATGGTTAC
CAGCCGGATGCTCGGCTGCTTCCTCGATGACGCGAGCAGCCGCAAGGAAGTTCTGGCCCTGATGGGACGGGGTTGA

Upstream 100 bases:

>100_bases
GGTATTCCAATGCGTGCCCTTTGGTCCGTGTTGGCAGGAAGCGGGGCGGGGCGCATCCGCAGGGCATCCCGCAACATTGC
CACACCGGAGCCTTGCCGCA

Downstream 100 bases:

>100_bases
TGGGACGGGGCTCAAACGGCTGAAAATGCAGATGCATGTTGCAATCGTTGGCGCGGGCATGGCCGGTCTTTCCTGCGCAA
GCCATCTCGTGCGTGCAGGT

Product: GTP cyclohydrolase

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNVLSPSLAASSGMADVIGEDGKIVVPADVEEAIRTLIRWSGDQPEREGLLDTPKRVARAWREYCSGYAQDPGLHLSRTF
EEVGGYDEVVLLRDIPFQSHCEHHMAPITGTASIAYLPRDRVVGISKLARVLHGFAQRLQVQERLTAEVAQCIWDNLKPH
GVAVVIEAQHGCMTGRGVRTHGVGMVTSRMLGCFLDDASSRKEVLALMGRG

Sequences:

>Translated_211_residues
MNVLSPSLAASSGMADVIGEDGKIVVPADVEEAIRTLIRWSGDQPEREGLLDTPKRVARAWREYCSGYAQDPGLHLSRTF
EEVGGYDEVVLLRDIPFQSHCEHHMAPITGTASIAYLPRDRVVGISKLARVLHGFAQRLQVQERLTAEVAQCIWDNLKPH
GVAVVIEAQHGCMTGRGVRTHGVGMVTSRMLGCFLDDASSRKEVLALMGRG
>Mature_211_residues
MNVLSPSLAASSGMADVIGEDGKIVVPADVEEAIRTLIRWSGDQPEREGLLDTPKRVARAWREYCSGYAQDPGLHLSRTF
EEVGGYDEVVLLRDIPFQSHCEHHMAPITGTASIAYLPRDRVVGISKLARVLHGFAQRLQVQERLTAEVAQCIWDNLKPH
GVAVVIEAQHGCMTGRGVRTHGVGMVTSRMLGCFLDDASSRKEVLALMGRG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=190, Percent_Identity=42.1052631578947, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI4503949, Length=190, Percent_Identity=42.1052631578947, Blast_Score=151, Evalue=4e-37,
Organism=Homo sapiens, GI66932970, Length=160, Percent_Identity=39.375, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI66932972, Length=150, Percent_Identity=41.3333333333333, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788476, Length=161, Percent_Identity=39.7515527950311, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=179, Percent_Identity=43.0167597765363, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=46.6666666666667, Blast_Score=156, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24656782, Length=182, Percent_Identity=43.4065934065934, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24656796, Length=182, Percent_Identity=43.4065934065934, Blast_Score=140, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24656791, Length=182, Percent_Identity=43.4065934065934, Blast_Score=140, Evalue=8e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23072; Mature: 23072

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLSPSLAASSGMADVIGEDGKIVVPADVEEAIRTLIRWSGDQPEREGLLDTPKRVARA
CCCCCCCHHHCCCCHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
WREYCSGYAQDPGLHLSRTFEEVGGYDEVVLLRDIPFQSHCEHHMAPITGTASIAYLPRD
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCC
RVVGISKLARVLHGFAQRLQVQERLTAEVAQCIWDNLKPHGVAVVIEAQHGCMTGRGVRT
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
HGVGMVTSRMLGCFLDDASSRKEVLALMGRG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNVLSPSLAASSGMADVIGEDGKIVVPADVEEAIRTLIRWSGDQPEREGLLDTPKRVARA
CCCCCCCHHHCCCCHHHHCCCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
WREYCSGYAQDPGLHLSRTFEEVGGYDEVVLLRDIPFQSHCEHHMAPITGTASIAYLPRD
HHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCCCEEEECCCC
RVVGISKLARVLHGFAQRLQVQERLTAEVAQCIWDNLKPHGVAVVIEAQHGCMTGRGVRT
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCC
HGVGMVTSRMLGCFLDDASSRKEVLALMGRG
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]