BacMap

Definition	Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome.
Accession	NC_007794
Length	3,561,584

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The map label for this gene is fabG [C]

Identifier: 87199247

GI number: 87199247

Start: 1278073

End: 1278990

Strand: Reverse

Name: fabG [C]

Synonym: Saro_1225

Alternate gene names: 87199247

Gene position: 1278990-1278073 (Counterclockwise)

Preceding gene: 87199250

Following gene: 87199246

Centisome position: 35.91

GC content: 65.14

Gene sequence:

>918_bases
ATGGCAGGCGTGCTTGAAGGCAAAGTCGTTGCCGTGACCGGGGCTGGACGCGGGATCGGACGGGAGATTGCCCTGCTTTG
CGCACGCGAAGGCGCGGCAGTCGTCGTCAACGATCCCGGCGTGGCGCAAGGCGGCGATGGCTCGGACGAAGGCCCGGCCC
AGACCACCGTCAACGACATCGTTGCGGCAGGCGGCAGAGCCTGGGCAAACCTTGCCAGCGTTGCCGATCCCATGGGCGCC
ACCAGCATCGTCGAGGATGCCGTGCAGCGCTTTGGCCGGATCGATGCGGTGGTCAACAATGCCGGCATCCTGCGCGACAC
GATCTGGCACAAGATGTCCTACGAGGACTGGACCAGCGTGATCGACGTGAACCTTACTGGCGCGTTCAACGTGTCGAAGG
CGGCGACTCCCTATTTCCGCGAGCAGTCTTCCGGCAGCTTCATCCACTTCACGTCCACCTCCGGCCTGATCGGCAACCTC
GGCCAGGCGAACTACTCGGCGGCAAAGCTGGGGCTGGTCGCGATGTCGCAATCCATCGCGCTCGACATGGCGCGCTGGGG
CGTGCGCTCCAACTGCATCGCGCCCTTCGCGTGGAGCCGCATGACCGCCTCGATCCCGGCCGAAACGCCAGAGCAGAAGC
AGCGCGTGGAACGGATGAAGACGATGAGCGCGGACAAGATCGCGCCGCTCGTCGCCTATCTCGCCTCGGACCTCTCGCAG
GACGTGACCAACCAGGTCTTCTCGGTCCGCAAGAACGAGATCGTGCTGTTCAGCAAGCCACGCCCGGTCCGCTCGATGGT
CAAGCTCGAAGGCTGGACGCCTTCCGCCATCGCCGAGGAACTGATCCCGGCGTTCAAGCCCGCATTCGCGCGGGCGGACG
AAGTCTCCGCCCACGTCTTCCCTTACGATCCGGTCTGA

Upstream 100 bases:

>100_bases
CGCGATGATACTTTTCATCATTATGAAAAGCAATTCTCGATGTTGAAAACTGTTAACTGTGCGGTTAGCGTCAGCCGAAG
AACTGAAACTGGAGATTCGA

Downstream 100 bases:

>100_bases
GGAGGCCTGAGATGAGCACGGTAAACCCTGGCATGGACGCCGAAATCTTCGACCAGTTCATCGAGCAGTTGCGCCGCTAC
GTACGCGAGCGGCTGATCCC

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAGVLEGKVVAVTGAGRGIGREIALLCAREGAAVVVNDPGVAQGGDGSDEGPAQTTVNDIVAAGGRAWANLASVADPMGA
TSIVEDAVQRFGRIDAVVNNAGILRDTIWHKMSYEDWTSVIDVNLTGAFNVSKAATPYFREQSSGSFIHFTSTSGLIGNL
GQANYSAAKLGLVAMSQSIALDMARWGVRSNCIAPFAWSRMTASIPAETPEQKQRVERMKTMSADKIAPLVAYLASDLSQ
DVTNQVFSVRKNEIVLFSKPRPVRSMVKLEGWTPSAIAEELIPAFKPAFARADEVSAHVFPYDPV

Sequences:

>Translated_305_residues
MAGVLEGKVVAVTGAGRGIGREIALLCAREGAAVVVNDPGVAQGGDGSDEGPAQTTVNDIVAAGGRAWANLASVADPMGA
TSIVEDAVQRFGRIDAVVNNAGILRDTIWHKMSYEDWTSVIDVNLTGAFNVSKAATPYFREQSSGSFIHFTSTSGLIGNL
GQANYSAAKLGLVAMSQSIALDMARWGVRSNCIAPFAWSRMTASIPAETPEQKQRVERMKTMSADKIAPLVAYLASDLSQ
DVTNQVFSVRKNEIVLFSKPRPVRSMVKLEGWTPSAIAEELIPAFKPAFARADEVSAHVFPYDPV
>Mature_304_residues
AGVLEGKVVAVTGAGRGIGREIALLCAREGAAVVVNDPGVAQGGDGSDEGPAQTTVNDIVAAGGRAWANLASVADPMGAT
SIVEDAVQRFGRIDAVVNNAGILRDTIWHKMSYEDWTSVIDVNLTGAFNVSKAATPYFREQSSGSFIHFTSTSGLIGNLG
QANYSAAKLGLVAMSQSIALDMARWGVRSNCIAPFAWSRMTASIPAETPEQKQRVERMKTMSADKIAPLVAYLASDLSQD
VTNQVFSVRKNEIVLFSKPRPVRSMVKLEGWTPSAIAEELIPAFKPAFARADEVSAHVFPYDPV

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=237, Percent_Identity=41.7721518987342, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI15277342, Length=251, Percent_Identity=34.6613545816733, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI40254992, Length=206, Percent_Identity=35.4368932038835, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI59889578, Length=168, Percent_Identity=32.1428571428571, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI10190704, Length=250, Percent_Identity=27.2, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=28.4, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4758504, Length=217, Percent_Identity=31.3364055299539, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40254986, Length=174, Percent_Identity=30.4597701149425, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI40807363, Length=223, Percent_Identity=25.5605381165919, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19923817, Length=253, Percent_Identity=24.901185770751, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI31542939, Length=243, Percent_Identity=27.9835390946502, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=25.390625, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI83715985, Length=198, Percent_Identity=31.3131313131313, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=36.6141732283465, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=31.8897637795276, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786812, Length=253, Percent_Identity=30.0395256916996, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=28.2868525896414, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=29.2682926829268, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=29.2181069958848, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082160, Length=213, Percent_Identity=29.1079812206573, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790717, Length=202, Percent_Identity=28.7128712871287, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787526, Length=262, Percent_Identity=32.0610687022901, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=26.8, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789057, Length=185, Percent_Identity=30.2702702702703, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=26.2548262548263, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=243, Percent_Identity=41.9753086419753, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI25147288, Length=254, Percent_Identity=35.8267716535433, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17555706, Length=250, Percent_Identity=30.8, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564282, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17561402, Length=254, Percent_Identity=29.1338582677165, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=26.7716535433071, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=257, Percent_Identity=28.0155642023346, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71982365, Length=140, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=248, Percent_Identity=25.8064516129032, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=249, Percent_Identity=28.1124497991968, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=218, Percent_Identity=28.4403669724771, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=248, Percent_Identity=27.8225806451613, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=207, Percent_Identity=30.9178743961353, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=205, Percent_Identity=27.8048780487805, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=199, Percent_Identity=28.643216080402, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=252, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=199, Percent_Identity=27.1356783919598, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=203, Percent_Identity=26.6009852216749, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=249, Percent_Identity=25.3012048192771, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=198, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=198, Percent_Identity=28.2828282828283, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536651, Length=251, Percent_Identity=25.4980079681275, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=203, Percent_Identity=26.1083743842365, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=244, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17558628, Length=198, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=199, Percent_Identity=28.1407035175879, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17551412, Length=196, Percent_Identity=28.0612244897959, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=246, Percent_Identity=43.4959349593496, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324126, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859837, Length=236, Percent_Identity=44.0677966101695, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=30.0395256916996, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24644339, Length=248, Percent_Identity=28.6290322580645, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357673, Length=197, Percent_Identity=30.4568527918782, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28571526, Length=189, Percent_Identity=31.2169312169312, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355319, Length=198, Percent_Identity=27.7777777777778, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17737361, Length=218, Percent_Identity=27.0642201834862, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=192, Percent_Identity=30.7291666666667, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640492, Length=196, Percent_Identity=25.5102040816327, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281364892, Length=211, Percent_Identity=27.4881516587678, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356301, Length=219, Percent_Identity=26.4840182648402, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24649181, Length=178, Percent_Identity=28.6516853932584, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 32416; Mature: 32285

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVLEGKVVAVTGAGRGIGREIALLCAREGAAVVVNDPGVAQGGDGSDEGPAQTTVNDI
CCCCCCCCEEEEECCCCCCCHHHHEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
VAAGGRAWANLASVADPMGATSIVEDAVQRFGRIDAVVNNAGILRDTIWHKMSYEDWTSV
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHEE
IDVNLTGAFNVSKAATPYFREQSSGSFIHFTSTSGLIGNLGQANYSAAKLGLVAMSQSIA
EEEEECCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LDMARWGVRSNCIAPFAWSRMTASIPAETPEQKQRVERMKTMSADKIAPLVAYLASDLSQ
HHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DVTNQVFSVRKNEIVLFSKPRPVRSMVKLEGWTPSAIAEELIPAFKPAFARADEVSAHVF
HHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEC
PYDPV
CCCCC
>Mature Secondary Structure 
AGVLEGKVVAVTGAGRGIGREIALLCAREGAAVVVNDPGVAQGGDGSDEGPAQTTVNDI
CCCCCCCEEEEECCCCCCCHHHHEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
VAAGGRAWANLASVADPMGATSIVEDAVQRFGRIDAVVNNAGILRDTIWHKMSYEDWTSV
HHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHEE
IDVNLTGAFNVSKAATPYFREQSSGSFIHFTSTSGLIGNLGQANYSAAKLGLVAMSQSIA
EEEEECCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LDMARWGVRSNCIAPFAWSRMTASIPAETPEQKQRVERMKTMSADKIAPLVAYLASDLSQ
HHHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
DVTNQVFSVRKNEIVLFSKPRPVRSMVKLEGWTPSAIAEELIPAFKPAFARADEVSAHVF
HHHHHHHHHCCCCEEEEECCCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEC
PYDPV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]