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The map label for this gene is fadE12 [H]

Identifier: 87199243

GI number: 87199243

Start: 1273185

End: 1274354

Strand: Reverse

Name: fadE12 [H]

Synonym: Saro_1221

Alternate gene names: 87199243

Gene position: 1274354-1273185 (Counterclockwise)

Preceding gene: 87199244

Following gene: 87199239

Centisome position: 35.78

GC content: 64.96

Gene sequence:

>1170_bases
ATGATCCTCTCGCCGTACGACAGCGAAGACCACGGCGAAATCCGTGAGGGCGTTCGCAAGCTATGCGCCCAGTTTCCGGG
CGAGTACTGGCGCAAGCTCGACGAGAAGCGCGAGTATCCGACCGACTTCGTCAACGCGCTGACCGAGGCGGGCTACCTTT
CCGTCCTCATCCCCGAGGAATACGGCGGCTCGGGCCTCGGCCTTGGTGCTGCGGCGGCGATCCTCGAGACGATCCAGTCG
GAGGGCGGCAACGGCGGCGCCTGTCATGCGCAGATGTACATCATGGGCACGATCCTTCGCCACGGGTCGGAAGAACAGAA
GCGCGCATACCTTCCCCGTATCGCCGCCGGGACCTTGCGCCTCCAGGCCTTCGGCGTGACCGAGCCGACCAGCGGCACCG
ACACCACCAGCTTGCGCACCTTCGCCCGCCGCGAGGGCGACGAATATGTCGTCAATGGCCAGAAGATCTGGACCAGCCGC
GCCGAACATTCCGATCTCATGCTCCTCCTCGCCCGCACCACCGCGCGCGAGGACGTGGCGAAGAAGACCGACGGCCTGTC
AGTCTTCATCGTCGACATGCGCGACGTCGTGGGCAAGTCGCTCACTATCCGCCCGATCCGCACGATGATGAACCACGCGA
CGACCGAGGTCTTCTTCGACAACATGCGCATTCCCGCCGCAAACCTTCTCGGCGAGGAGGGCAAGGGCTTCCGTTACATC
CTGTCGGGCATGAACGCCGAACGGGTGCTGATCGCGGCGGAATGCGTGGGCGACGCCAAGTGGTTCACGCGGCGGTCGGT
CGAATATGCCGGGGAGCGAAAGGTCTTCGGCCGTGCCATCGGCGCCAATCAGGGCGTCGCCTTCCCCATCGCCAAGGCCT
ACGCAAACATGCGCGCCGCCGAACTCATGGTGCGCGAAGCCGCGCGCATGTACGAGGCGGGTTTGCCTATGGGCGCGGAA
GCGAACATGGCCAAGATGCTTGCGGCTGATGCATCATGGGAAGCCGGCAACGCCTGCGTCCAGACGCACGGCGGCTTCGG
CTTTGCCGAGGAGTACGACATCGAACGCAAGCTGCGCGAGACCCGGCTCTACCAGGTGGCGCCGATCAGCACGAACCTGA
TCCTGTCGTTCCTGGCCGAACACGTTCTGGGCCTGCCCCGCAGCTACTGA

Upstream 100 bases:

>100_bases
ACCTTACGCGCGAATTGAAGCGGCGCGGCGGGCGCTATGGCCTTGAAACCATGTGCATTGGTGGCGGTCAGGGAATCGCG
GCGGTGTTCGAGGCGGCCTG

Downstream 100 bases:

>100_bases
GGCTTCGTCACTTCCACCAATCCGCCTCCCAGCGCACGGTACAGCTCGACCATGTTGCTCGCTTTCGTGAGCCGCACCGA
AACCAGCGACTGCCGCGCAT

Product: acyl-CoA dehydrogenase-like protein

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MILSPYDSEDHGEIREGVRKLCAQFPGEYWRKLDEKREYPTDFVNALTEAGYLSVLIPEEYGGSGLGLGAAAAILETIQS
EGGNGGACHAQMYIMGTILRHGSEEQKRAYLPRIAAGTLRLQAFGVTEPTSGTDTTSLRTFARREGDEYVVNGQKIWTSR
AEHSDLMLLLARTTAREDVAKKTDGLSVFIVDMRDVVGKSLTIRPIRTMMNHATTEVFFDNMRIPAANLLGEEGKGFRYI
LSGMNAERVLIAAECVGDAKWFTRRSVEYAGERKVFGRAIGANQGVAFPIAKAYANMRAAELMVREAARMYEAGLPMGAE
ANMAKMLAADASWEAGNACVQTHGGFGFAEEYDIERKLRETRLYQVAPISTNLILSFLAEHVLGLPRSY

Sequences:

>Translated_389_residues
MILSPYDSEDHGEIREGVRKLCAQFPGEYWRKLDEKREYPTDFVNALTEAGYLSVLIPEEYGGSGLGLGAAAAILETIQS
EGGNGGACHAQMYIMGTILRHGSEEQKRAYLPRIAAGTLRLQAFGVTEPTSGTDTTSLRTFARREGDEYVVNGQKIWTSR
AEHSDLMLLLARTTAREDVAKKTDGLSVFIVDMRDVVGKSLTIRPIRTMMNHATTEVFFDNMRIPAANLLGEEGKGFRYI
LSGMNAERVLIAAECVGDAKWFTRRSVEYAGERKVFGRAIGANQGVAFPIAKAYANMRAAELMVREAARMYEAGLPMGAE
ANMAKMLAADASWEAGNACVQTHGGFGFAEEYDIERKLRETRLYQVAPISTNLILSFLAEHVLGLPRSY
>Mature_389_residues
MILSPYDSEDHGEIREGVRKLCAQFPGEYWRKLDEKREYPTDFVNALTEAGYLSVLIPEEYGGSGLGLGAAAAILETIQS
EGGNGGACHAQMYIMGTILRHGSEEQKRAYLPRIAAGTLRLQAFGVTEPTSGTDTTSLRTFARREGDEYVVNGQKIWTSR
AEHSDLMLLLARTTAREDVAKKTDGLSVFIVDMRDVVGKSLTIRPIRTMMNHATTEVFFDNMRIPAANLLGEEGKGFRYI
LSGMNAERVLIAAECVGDAKWFTRRSVEYAGERKVFGRAIGANQGVAFPIAKAYANMRAAELMVREAARMYEAGLPMGAE
ANMAKMLAADASWEAGNACVQTHGGFGFAEEYDIERKLRETRLYQVAPISTNLILSFLAEHVLGLPRSY

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=375, Percent_Identity=30.1333333333333, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI4501857, Length=378, Percent_Identity=30.1587301587302, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI226958412, Length=365, Percent_Identity=27.1232876712329, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4557233, Length=364, Percent_Identity=30.2197802197802, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI7656849, Length=354, Percent_Identity=29.0960451977401, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI21361497, Length=365, Percent_Identity=30.1369863013699, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI187960098, Length=357, Percent_Identity=29.4117647058824, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4557231, Length=357, Percent_Identity=29.4117647058824, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI226958414, Length=337, Percent_Identity=28.1899109792285, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4557235, Length=348, Percent_Identity=31.3218390804598, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI76496475, Length=348, Percent_Identity=31.3218390804598, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI38505218, Length=293, Percent_Identity=26.2798634812287, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI7669494, Length=341, Percent_Identity=26.3929618768328, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4503943, Length=323, Percent_Identity=26.9349845201238, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786223, Length=373, Percent_Identity=29.7587131367292, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081958, Length=389, Percent_Identity=26.7352185089974, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17508101, Length=380, Percent_Identity=31.3157894736842, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17506239, Length=373, Percent_Identity=30.2949061662198, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71985184, Length=356, Percent_Identity=32.3033707865169, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17569725, Length=369, Percent_Identity=31.7073170731707, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17534899, Length=349, Percent_Identity=34.3839541547278, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17570075, Length=382, Percent_Identity=29.3193717277487, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71990804, Length=314, Percent_Identity=33.4394904458599, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17538396, Length=359, Percent_Identity=26.1838440111421, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17533517, Length=355, Percent_Identity=28.169014084507, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71985192, Length=280, Percent_Identity=32.8571428571429, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17551932, Length=368, Percent_Identity=25.8152173913043, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17505929, Length=369, Percent_Identity=26.8292682926829, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32563615, Length=232, Percent_Identity=31.0344827586207, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982178, Length=273, Percent_Identity=27.8388278388278, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17531909, Length=246, Percent_Identity=28.8617886178862, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17534353, Length=270, Percent_Identity=25.9259259259259, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17531913, Length=167, Percent_Identity=26.9461077844311, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17562446, Length=273, Percent_Identity=24.9084249084249, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI86563383, Length=274, Percent_Identity=27.3722627737226, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI86563381, Length=274, Percent_Identity=27.3722627737226, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24666513, Length=387, Percent_Identity=31.7829457364341, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21356377, Length=363, Percent_Identity=29.2011019283747, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24660351, Length=366, Percent_Identity=30.0546448087432, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24646207, Length=378, Percent_Identity=26.4550264550265, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281363737, Length=343, Percent_Identity=30.3206997084548, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI21355753, Length=339, Percent_Identity=27.4336283185841, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI19920834, Length=269, Percent_Identity=29.7397769516729, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42846; Mature: 42846

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00072 ACYL_COA_DH_1 ; PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSPYDSEDHGEIREGVRKLCAQFPGEYWRKLDEKREYPTDFVNALTEAGYLSVLIPEE
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECC
YGGSGLGLGAAAAILETIQSEGGNGGACHAQMYIMGTILRHGSEEQKRAYLPRIAAGTLR
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEE
LQAFGVTEPTSGTDTTSLRTFARREGDEYVVNGQKIWTSRAEHSDLMLLLARTTAREDVA
EEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCEEEEEHHHHHHHHHH
KKTDGLSVFIVDMRDVVGKSLTIRPIRTMMNHATTEVFFDNMRIPAANLLGEEGKGFRYI
HHCCCCEEEEEECHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHH
LSGMNAERVLIAAECVGDAKWFTRRSVEYAGERKVFGRAIGANQGVAFPIAKAYANMRAA
CCCCCCCEEEEEEHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELMVREAARMYEAGLPMGAEANMAKMLAADASWEAGNACVQTHGGFGFAEEYDIERKLRE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
TRLYQVAPISTNLILSFLAEHVLGLPRSY
HHCEEECCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILSPYDSEDHGEIREGVRKLCAQFPGEYWRKLDEKREYPTDFVNALTEAGYLSVLIPEE
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECC
YGGSGLGLGAAAAILETIQSEGGNGGACHAQMYIMGTILRHGSEEQKRAYLPRIAAGTLR
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEE
LQAFGVTEPTSGTDTTSLRTFARREGDEYVVNGQKIWTSRAEHSDLMLLLARTTAREDVA
EEEECCCCCCCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCCCCEEEEEHHHHHHHHHH
KKTDGLSVFIVDMRDVVGKSLTIRPIRTMMNHATTEVFFDNMRIPAANLLGEEGKGFRYI
HHCCCCEEEEEECHHHHCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHH
LSGMNAERVLIAAECVGDAKWFTRRSVEYAGERKVFGRAIGANQGVAFPIAKAYANMRAA
CCCCCCCEEEEEEHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELMVREAARMYEAGLPMGAEANMAKMLAADASWEAGNACVQTHGGFGFAEEYDIERKLRE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
TRLYQVAPISTNLILSFLAEHVLGLPRSY
HHCEEECCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]