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The map label for this gene is leuC

Identifier: 87199192

GI number: 87199192

Start: 1210614

End: 1212050

Strand: Direct

Name: leuC

Synonym: Saro_1170

Alternate gene names: 87199192

Gene position: 1210614-1212050 (Clockwise)

Preceding gene: 87199191

Following gene: 87199193

Centisome position: 33.99

GC content: 68.34

Gene sequence:

>1437_bases
ATGTCCAGCACCGCACCCCGCACGCTCTACCAGAAGATCTGGGACGCCCACGTCGTCGAACGCCGTGATGATGGCACCTG
CCTCATCTACATCGACCGTCACCTCGTCCACGAAGTGACCAGCCCGCAGGCCTTCGAGGCGCTTCGCGCCGCCGGCCGCA
AGGTGCGCCGTCCCGATCTCACGCTTGCGGTGCCAGACCACAACCTGCCGACCACCGCGCGCCGTACCGCCGATGGCCGG
CGCGTGCCCATCGCCGATCCCGAATCGGCCCAGCAGCTCGAGGCGCTGGAGCGCAACGCCCCCGAATTCGGCATCCGCTA
TATCGGCGATGCCGATGACGAGCAGGGCATCGTCCACGTCGTCGGCCCGGAACAGGGCTTCTCGCTCCCCGGCGCGACGA
TCGTCTGCGGCGACAGCCACACCGCCTGCCACGGCGGCCTGGGTGCGCTGGCCTTCGGCATCGGCACGAGCGAGGTCGAG
CACGTCCTCGCCACGCAGACCCTGCTGCTCAAGCAGTCGAAGACGATGGAAGTGCGCGTCGAGGGCGAACTGACCCCGGG
CGTCACGGCCAAGGATGTCGTCCTGCACATCACCGGCGTGCTCGGCGCGGCTGGCGGCACCGGCTCGGTCATCGAGTACA
CCGGCTCCGTCATCCGCGACCTGTCGATCGAGGGTCGCCTGACCATCTCCAACATGGCGATCGAGCACGGCGCGCGCGCG
GGCCTTTGCGCTCCGGACGAAAAGACCTTCGCCTATCTCAAGGGCCGTCCCTACGCGCCCAGGGGCGAGGACTGGGACAA
GGCCGTCGCGTGGTGGAAGAGCCTCGCGACAGATCCCGGCGCGACCTATGACAAGGTCGTCGTGATCGACGCGAAGGACA
TCGCTCCTTCCGTCACCTGGGGCACCAGCCCGGAAGACGTGCTGCCGATCTCCGGCCTCGTCCCCGCGCCTGAATCCTTC
GCAGATCCCTCCAAGCAGGAAGCCGCCCGCGCGAGCCTCGAATACATGGGCCTCGTTCCCGGCCAGCGCATGGAGGACGT
CGAGGTGCAGAACATCTTCATCGGCTCGTGCACCAACAGCCGCATCGAGGACATGCGCGCCGCTGCCGCGATCCTGAAGG
GCCGCAAGAAGGCGGACAACGTGAAGTGGGCCATCGTGGTGCCCGGCTCGGGGCTGGTGAAGAAGCAGGCGGAAGAGGAA
GGCCTCGACCGCGTGTTCATCGAAGCCGGCTTCGAATGGCGCGAGCCCGGATGTTCGGCCTGTCTCGGCATGAACCCGGA
CAAGGTGCCAGCGGGCGAACGCTGCGCTTCGACCTCCAACCGCAACTTCGTCGGCCGCCAGGGCCCCGGCGCGCGCACGC
ACCTCGTCAGCCCGGCGATGGCGGCGGCCGCTGCCGTTACCGGCAGGCTGACGGACGTGCGCAAGCTGATGGCCTGA

Upstream 100 bases:

>100_bases
CGAAAGTAAGCGCGCGGCGTAACAAAATTGCGCCGCGCCGCATCAAGGCCCGCCCTTTCCTGCTTGAATTGCAGCAAAGG
CGGGCCTAACCGCTTGATCC

Downstream 100 bases:

>100_bases
CGCGGAAATGCCGGCACTTCCGACAGCCGCGCTCCAGCTCGCGGGGTTCCTCATGGCCCATGCGTTCTGGACGGCGTCGG
ATCTGCCGCCCGGCGGCCAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSSTAPRTLYQKIWDAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEALRAAGRKVRRPDLTLAVPDHNLPTTARRTADGR
RVPIADPESAQQLEALERNAPEFGIRYIGDADDEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVE
HVLATQTLLLKQSKTMEVRVEGELTPGVTAKDVVLHITGVLGAAGGTGSVIEYTGSVIRDLSIEGRLTISNMAIEHGARA
GLCAPDEKTFAYLKGRPYAPRGEDWDKAVAWWKSLATDPGATYDKVVVIDAKDIAPSVTWGTSPEDVLPISGLVPAPESF
ADPSKQEAARASLEYMGLVPGQRMEDVEVQNIFIGSCTNSRIEDMRAAAAILKGRKKADNVKWAIVVPGSGLVKKQAEEE
GLDRVFIEAGFEWREPGCSACLGMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRKLMA

Sequences:

>Translated_478_residues
MSSTAPRTLYQKIWDAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEALRAAGRKVRRPDLTLAVPDHNLPTTARRTADGR
RVPIADPESAQQLEALERNAPEFGIRYIGDADDEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVE
HVLATQTLLLKQSKTMEVRVEGELTPGVTAKDVVLHITGVLGAAGGTGSVIEYTGSVIRDLSIEGRLTISNMAIEHGARA
GLCAPDEKTFAYLKGRPYAPRGEDWDKAVAWWKSLATDPGATYDKVVVIDAKDIAPSVTWGTSPEDVLPISGLVPAPESF
ADPSKQEAARASLEYMGLVPGQRMEDVEVQNIFIGSCTNSRIEDMRAAAAILKGRKKADNVKWAIVVPGSGLVKKQAEEE
GLDRVFIEAGFEWREPGCSACLGMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRKLMA
>Mature_477_residues
SSTAPRTLYQKIWDAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEALRAAGRKVRRPDLTLAVPDHNLPTTARRTADGRR
VPIADPESAQQLEALERNAPEFGIRYIGDADDEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEH
VLATQTLLLKQSKTMEVRVEGELTPGVTAKDVVLHITGVLGAAGGTGSVIEYTGSVIRDLSIEGRLTISNMAIEHGARAG
LCAPDEKTFAYLKGRPYAPRGEDWDKAVAWWKSLATDPGATYDKVVVIDAKDIAPSVTWGTSPEDVLPISGLVPAPESFA
DPSKQEAARASLEYMGLVPGQRMEDVEVQNIFIGSCTNSRIEDMRAAAAILKGRKKADNVKWAIVVPGSGLVKKQAEEEG
LDRVFIEAGFEWREPGCSACLGMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRKLMA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=28.099173553719, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI8659555, Length=456, Percent_Identity=27.6315789473684, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=28.1984334203655, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=61.1464968152866, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1787531, Length=368, Percent_Identity=28.2608695652174, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081781, Length=356, Percent_Identity=26.123595505618, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI2367097, Length=373, Percent_Identity=28.1501340482574, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=30.6629834254144, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=30.6629834254144, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17568399, Length=442, Percent_Identity=28.9592760180996, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=29.8701298701299, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=59.704641350211, Blast_Score=600, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=31.5934065934066, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=499, Percent_Identity=28.4569138276553, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320440, Length=434, Percent_Identity=24.6543778801843, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=31.129476584022, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281365315, Length=363, Percent_Identity=31.6804407713499, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17864292, Length=363, Percent_Identity=31.6804407713499, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=31.6804407713499, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=29.3800539083558, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=29.4594594594595, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_NOVAD (Q2G958)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496449.1
- STRING:   Q2G958
- GeneID:   3916467
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1170
- NMPDR:   fig|48935.1.peg.2008
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q2G958
- ProtClustDB:   PRK05478
- BioCyc:   NARO279238:SARO_1170-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51126; Mature: 50995

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTAPRTLYQKIWDAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEALRAAGRKVRRPDL
CCCCCHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCE
TLAVPDHNLPTTARRTADGRRVPIADPESAQQLEALERNAPEFGIRYIGDADDEQGIVHV
EEEECCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
VGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEHVLATQTLLLKQSKTMEVRV
ECCCCCCCCCCCEEEECCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
EGELTPGVTAKDVVLHITGVLGAAGGTGSVIEYTGSVIRDLSIEGRLTISNMAIEHGARA
ECCCCCCCCHHHHHEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCC
GLCAPDEKTFAYLKGRPYAPRGEDWDKAVAWWKSLATDPGATYDKVVVIDAKDIAPSVTW
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHCCCCCCC
GTSPEDVLPISGLVPAPESFADPSKQEAARASLEYMGLVPGQRMEDVEVQNIFIGSCTNS
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCH
RIEDMRAAAAILKGRKKADNVKWAIVVPGSGLVKKQAEEEGLDRVFIEAGFEWREPGCSA
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCE
CLGMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRKLMA
ECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSTAPRTLYQKIWDAHVVERRDDGTCLIYIDRHLVHEVTSPQAFEALRAAGRKVRRPDL
CCCCHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHCCCCHHHHHHHHHCCCCCCCCE
TLAVPDHNLPTTARRTADGRRVPIADPESAQQLEALERNAPEFGIRYIGDADDEQGIVHV
EEEECCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEE
VGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEHVLATQTLLLKQSKTMEVRV
ECCCCCCCCCCCEEEECCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEE
EGELTPGVTAKDVVLHITGVLGAAGGTGSVIEYTGSVIRDLSIEGRLTISNMAIEHGARA
ECCCCCCCCHHHHHEEEEHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCC
GLCAPDEKTFAYLKGRPYAPRGEDWDKAVAWWKSLATDPGATYDKVVVIDAKDIAPSVTW
CCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECHHCCCCCCC
GTSPEDVLPISGLVPAPESFADPSKQEAARASLEYMGLVPGQRMEDVEVQNIFIGSCTNS
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHEECCCCCCCCCCEEEEEEEECCCCH
RIEDMRAAAAILKGRKKADNVKWAIVVPGSGLVKKQAEEEGLDRVFIEAGFEWREPGCSA
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCE
CLGMNPDKVPAGERCASTSNRNFVGRQGPGARTHLVSPAMAAAAAVTGRLTDVRKLMA
ECCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA