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The map label for this gene is miaB

Identifier: 87199004

GI number: 87199004

Start: 1025606

End: 1026937

Strand: Reverse

Name: miaB

Synonym: Saro_0982

Alternate gene names: 87199004

Gene position: 1026937-1025606 (Counterclockwise)

Preceding gene: 87199005

Following gene: 87199003

Centisome position: 28.83

GC content: 66.44

Gene sequence:

>1332_bases
ATGTCCTCCTCCGCACCTTCCTCCGCATCTCCCAAGACGTTCCGCGTCAAAAGCTTCGGCTGCCAGATGAACGTCTACGA
CGGCGAGCGCATGGCAGAGCTACTTGCGGCGCAAGGGATGTCGGCCGCAGGGGACGGGGACGATGCCGACCTGGTCGTGC
TCAACACGTGCCACATCCGCGAAAAGGCCACCGAAAAGGTCTATTCCGACATCGGTCGCCTGCGCCGCGCGGACGGCTCT
GCCCCGCTCATCGCCGTGGCGGGGTGCGTCGCCCAGGCCGAGGGCGAGGAGATCATGGCCCGCGCGCCAAGCGTAAAGGT
CGTGGTCGGCCCGCAGTCGTATCACCGCCTGCCCGAAATGGTCGCCGACGCTGCTGCCGGCAAGCGCTCGACCGAAACCG
ACATGCCCGCCGAGGCCAAGTTCGCGGCATTGCCCAAGCGCCGCAAGTCTGCGCCGACCGCGTTCCTTACCGTGCAGGAA
GGTTGCGACAAGTTCTGCACCTATTGCGTCGTGCCCTATACGCGCGGGGCCGAAATCAGCCGGCCGTTCTCCGATCTGGT
AGAGGAGGCAAAGCTGCTCGTCGCAGGCGGCGCGCGCGAGATCACGCTGCTCGGCCAGAACGTCAACGCCTGGGCCGGCG
AGGACGACAAGGGCCGCCCCATCGGGCTCGACGGTCTTGCCCGCGCGCTGGCCGCCGAACCAGACCTCAAGCGCATCCGC
TATACCACCAGCCATCCCAACGACATGACCGACGGCCTGATCGCCGCGCATGGCGAACTCGAAAAGCTGATGCCGTTCCT
GCACCTGCCCGTCCAGGCCGGTAACGACCGCGTGCTCAAGGCGATGAACCGCAGCCATACCGCCGACAGCTACATGGCGC
TGCTTGAACGCATCCGCGCCGCGCGGCCGGACATCGCGCTTTCGGGCGACTTCATCGTCGGTTTCCCCGGCGAGACCGAC
GCCGAATTCGAGGACACGCTGCGCCTGGTCGATGCGGTGGGCTATGCTCAGGCCTTCAGCTTCAAGTATTCCGCCCGCCC
CGGCACTCCGGCGGCGACGATGGAGAACCACGTTCCCGTCGCCGTCATGGACGAACGCCTCCAGCGCCTCCAGGCCGCGC
TCAACCGGGATCAACTCGCCTTCAACAAGGCCAGCGTCGGCAAGACGTGCGAGGTTCTGGTCGAACGCCGGGGCAAGCAT
CCCGGCCAGTGGCTCGGCAAATCGCCCTGGCTCCAATCGGTGCACTTTTCCGGCGAGGCGGAAATCGGGGACATGGTCAC
AGTGGAACTGATCGAGGCCGGGCCGAACTCGATTTCCGGGCGCCTGGCCTGA

Upstream 100 bases:

>100_bases
AGCCGCAAACTGATGGCAACTGCCTCGCGTGAACGGATCCTCGCCGCGATGGGCCGCAACAGGTAGCCAAGCCTCGCCAA
ATCCAGTATCGGCGCGGCCC

Downstream 100 bases:

>100_bases
ACCGCGCCCCTGCCTGAAAGACACCCTGCCGAACGCTTCGTCCAATTGGTCTGTGGAATAGTCGGCGCGGCGCTTGCGCC
GGCAATATCCATACCTATTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIREKATEKVYSDIGRLRRADGS
APLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEMVADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQE
GCDKFCTYCVVPYTRGAEISRPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIR
YTTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRAARPDIALSGDFIVGFPGETD
AEFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPVAVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKH
PGQWLGKSPWLQSVHFSGEAEIGDMVTVELIEAGPNSISGRLA

Sequences:

>Translated_443_residues
MSSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIREKATEKVYSDIGRLRRADGS
APLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEMVADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQE
GCDKFCTYCVVPYTRGAEISRPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIR
YTTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRAARPDIALSGDFIVGFPGETD
AEFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPVAVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKH
PGQWLGKSPWLQSVHFSGEAEIGDMVTVELIEAGPNSISGRLA
>Mature_442_residues
SSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIREKATEKVYSDIGRLRRADGSA
PLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEMVADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQEG
CDKFCTYCVVPYTRGAEISRPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIRY
TTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRAARPDIALSGDFIVGFPGETDA
EFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPVAVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKHP
GQWLGKSPWLQSVHFSGEAEIGDMVTVELIEAGPNSISGRLA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=33.0526315789474, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI28872782, Length=414, Percent_Identity=34.7826086956522, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI93277076, Length=438, Percent_Identity=28.5388127853881, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=46.0136674259681, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787057, Length=441, Percent_Identity=29.0249433106576, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17553146, Length=416, Percent_Identity=34.8557692307692, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=276, Percent_Identity=31.8840579710145, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21356207, Length=469, Percent_Identity=32.8358208955224, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI19922432, Length=374, Percent_Identity=30.2139037433155, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NOVAD (Q2G9P6)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496261.1
- ProteinModelPortal:   Q2G9P6
- SMR:   Q2G9P6
- STRING:   Q2G9P6
- GeneID:   3915764
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0982
- NMPDR:   fig|48935.1.peg.3088
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- PhylomeDB:   Q2G9P6
- ProtClustDB:   PRK14325
- BioCyc:   NARO279238:SARO_0982-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 47686; Mature: 47554

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIR
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHH
EKATEKVYSDIGRLRRADGSAPLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHH
VADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQEGCDKFCTYCVVPYTRGAEIS
HHHHHCCCCCCCCCCCCCHHHHHCCCHHCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCC
RPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIR
CCHHHHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHCCCCHHHEE
YTTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRA
EECCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ARPDIALSGDFIVGFPGETDAEFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPV
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCH
AVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKHPGQWLGKSPWLQSVHFSGEA
HHHHHHHHHHHHHHCHHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCC
EIGDMVTVELIEAGPNSISGRLA
CCCCEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SSSAPSSASPKTFRVKSFGCQMNVYDGERMAELLAAQGMSAAGDGDDADLVVLNTCHIR
CCCCCCCCCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHH
EKATEKVYSDIGRLRRADGSAPLIAVAGCVAQAEGEEIMARAPSVKVVVGPQSYHRLPEM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHHH
VADAAAGKRSTETDMPAEAKFAALPKRRKSAPTAFLTVQEGCDKFCTYCVVPYTRGAEIS
HHHHHCCCCCCCCCCCCCHHHHHCCCHHCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCC
RPFSDLVEEAKLLVAGGAREITLLGQNVNAWAGEDDKGRPIGLDGLARALAAEPDLKRIR
CCHHHHHHHHHEEEECCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHCCCCHHHEE
YTTSHPNDMTDGLIAAHGELEKLMPFLHLPVQAGNDRVLKAMNRSHTADSYMALLERIRA
EECCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
ARPDIALSGDFIVGFPGETDAEFEDTLRLVDAVGYAQAFSFKYSARPGTPAATMENHVPV
CCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCH
AVMDERLQRLQAALNRDQLAFNKASVGKTCEVLVERRGKHPGQWLGKSPWLQSVHFSGEA
HHHHHHHHHHHHHHCHHHHHCCHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHEECCCCC
EIGDMVTVELIEAGPNSISGRLA
CCCCEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA