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The map label for this gene is gcvA [H]

Identifier: 87199000

GI number: 87199000

Start: 1022068

End: 1022949

Strand: Reverse

Name: gcvA [H]

Synonym: Saro_0978

Alternate gene names: 87199000

Gene position: 1022949-1022068 (Counterclockwise)

Preceding gene: 87199001

Following gene: 87198999

Centisome position: 28.72

GC content: 64.4

Gene sequence:

>882_bases
ATGATCCGCCGCTTGCCCCCGCTGCGCTCGCTCGAGGCGTTCATTCGCGTCGTGCGCGCGGGTTCGGCCAAGACCGCGGC
GGCGGAACTCGCGCTCAGCCCTTCGGCCCTGTCGCGCAGGCTCGGCGCGCTCGAGGAATTCATCGGCAAGCCGCTGTTCG
AACGCAAGCACCAGGCGCTCAAGCTGACCGACGAGGGCCAGCAGCTCTATGATGCCGTCGCCCCGCTGATCGATGAAATG
GCCGACCGGGTGGATCGCCTGATCGATACCGGCAAGGTCATGCGTCTTCGCCTCGGCGTGCTTCCGCTGTTCGGCAGCCA
GCGCCTGTTCCCGCGCCTGGGCGAACTGCGCAAGCTGCATCCGCAACTGCACATCGACATCGATTCCGGCCACGCCGCCG
AAACCAAGCTGGGCGACACGATCGATGCCGCCATCGTCCTTTCCGAAGGACCGGACGCCTCGCTCCATTCGGTGCGCCTC
GATCACAACCGTGTCCACGCCATCACTTCGAAGCAACTGGCCGACGAACTGGGCGACAAGCCGGACATCGCCAAGCTCTC
GAAGCAGACGTTCCTCGTTCACAACGACCTGCCGATGAGCTTCGAGGCATGGAAGAAGGCGCTGCACCTCGAAAAGCTCG
AACCAGCAGCCATCGACCATTTCGATTCGGGCCAGCTCATCCTCGAAGCGGCGGCGCAGGGCCTCGGCATCGCGATCATG
CACGACGACCATTATCATCGCAGCCATGATCCGCGCCTCGCGCGTCTCTACAACATCGATGTCGACAGCCCCTATTCCTA
CTGGTTCGTCTGCCGTCCCCGCGCGCTCCAGTCGCGCCCGGTCCGGTTGTTCCACGACTGGATGCTGAAGGCGGGGCTTT
GA

Upstream 100 bases:

>100_bases
CCAGCGGTTGGCGCATCGAGGTCACGGGTGGCGACGAACGTCGCCTCACGCGCCTCAGGCTCCATGCGCCGCAGGCCGAA
CCGGTTGACGAGGAATAACG

Downstream 100 bases:

>100_bases
CGCTCGCACGCCGCGCGGCCATCGCGGTCAAGATCCATTGCCCGGTCTCTGCCGCGACGCTGGCAGCAATGCTCGCCGGC
GATGCCTCCGCGATCGAGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MIRRLPPLRSLEAFIRVVRAGSAKTAAAELALSPSALSRRLGALEEFIGKPLFERKHQALKLTDEGQQLYDAVAPLIDEM
ADRVDRLIDTGKVMRLRLGVLPLFGSQRLFPRLGELRKLHPQLHIDIDSGHAAETKLGDTIDAAIVLSEGPDASLHSVRL
DHNRVHAITSKQLADELGDKPDIAKLSKQTFLVHNDLPMSFEAWKKALHLEKLEPAAIDHFDSGQLILEAAAQGLGIAIM
HDDHYHRSHDPRLARLYNIDVDSPYSYWFVCRPRALQSRPVRLFHDWMLKAGL

Sequences:

>Translated_293_residues
MIRRLPPLRSLEAFIRVVRAGSAKTAAAELALSPSALSRRLGALEEFIGKPLFERKHQALKLTDEGQQLYDAVAPLIDEM
ADRVDRLIDTGKVMRLRLGVLPLFGSQRLFPRLGELRKLHPQLHIDIDSGHAAETKLGDTIDAAIVLSEGPDASLHSVRL
DHNRVHAITSKQLADELGDKPDIAKLSKQTFLVHNDLPMSFEAWKKALHLEKLEPAAIDHFDSGQLILEAAAQGLGIAIM
HDDHYHRSHDPRLARLYNIDVDSPYSYWFVCRPRALQSRPVRLFHDWMLKAGL
>Mature_293_residues
MIRRLPPLRSLEAFIRVVRAGSAKTAAAELALSPSALSRRLGALEEFIGKPLFERKHQALKLTDEGQQLYDAVAPLIDEM
ADRVDRLIDTGKVMRLRLGVLPLFGSQRLFPRLGELRKLHPQLHIDIDSGHAAETKLGDTIDAAIVLSEGPDASLHSVRL
DHNRVHAITSKQLADELGDKPDIAKLSKQTFLVHNDLPMSFEAWKKALHLEKLEPAAIDHFDSGQLILEAAAQGLGIAIM
HDDHYHRSHDPRLARLYNIDVDSPYSYWFVCRPRALQSRPVRLFHDWMLKAGL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=26.4406779661017, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786448, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789440, Length=244, Percent_Identity=24.1803278688525, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788706, Length=239, Percent_Identity=26.3598326359833, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=26.2975778546713, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789639, Length=193, Percent_Identity=24.8704663212435, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081978, Length=245, Percent_Identity=24.8979591836735, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32900; Mature: 32900

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRLPPLRSLEAFIRVVRAGSAKTAAAELALSPSALSRRLGALEEFIGKPLFERKHQAL
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
KLTDEGQQLYDAVAPLIDEMADRVDRLIDTGKVMRLRLGVLPLFGSQRLFPRLGELRKLH
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLHIDIDSGHAAETKLGDTIDAAIVLSEGPDASLHSVRLDHNRVHAITSKQLADELGDK
CEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
PDIAKLSKQTFLVHNDLPMSFEAWKKALHLEKLEPAAIDHFDSGQLILEAAAQGLGIAIM
CCHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHCCCEEEEE
HDDHYHRSHDPRLARLYNIDVDSPYSYWFVCRPRALQSRPVRLFHDWMLKAGL
ECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIRRLPPLRSLEAFIRVVRAGSAKTAAAELALSPSALSRRLGALEEFIGKPLFERKHQAL
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHE
KLTDEGQQLYDAVAPLIDEMADRVDRLIDTGKVMRLRLGVLPLFGSQRLFPRLGELRKLH
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
PQLHIDIDSGHAAETKLGDTIDAAIVLSEGPDASLHSVRLDHNRVHAITSKQLADELGDK
CEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
PDIAKLSKQTFLVHNDLPMSFEAWKKALHLEKLEPAAIDHFDSGQLILEAAAQGLGIAIM
CCHHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHCCCEEEEE
HDDHYHRSHDPRLARLYNIDVDSPYSYWFVCRPRALQSRPVRLFHDWMLKAGL
ECCCCCCCCCCCEEEEEECCCCCCCCEEEEECCHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]