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The map label for this gene is ksgA

Identifier: 87198908

GI number: 87198908

Start: 941886

End: 942707

Strand: Reverse

Name: ksgA

Synonym: Saro_0886

Alternate gene names: 87198908

Gene position: 942707-941886 (Counterclockwise)

Preceding gene: 87198909

Following gene: 87198906

Centisome position: 26.47

GC content: 67.03

Gene sequence:

>822_bases
ATGACCGACCTTCCCCCCCTGCGCGACGTCGTCAACCGTCACGGCCTCTACGCAACCAAGGCCCTGGGGCAGAACTTCCT
GTTCGACGAGCAGTTGCTCGACCGCATCGCCCGCGTGCCGGGCAAGCTCAAGGGCGAGAACGTGCTCGAGGTCGGCCCCG
GCCCCGGCGGCCTGACCCGCGCCCTGCTGCGCGCGGGGGCAAAGGTCACTGCCATCGAGATGGACAAGCGCTGCCTTCCC
GCGCTGGCCGAACTGGCCGATGCCTTTCCAGGCCAACTGACAGTGATCGAAGGCGACGCGACCAAGATTGCGCCCGAAAC
CCTGTTCGACGGCCCCTGGCACGTCGCCGCCAACCTGCCCTACAACGTCGGCACGCAGCTTTTTACCGGCTGGCTTTCCG
GACAGGACTGGCCGCCGCAGTGGAAATCGCTGACGCTGATGTTCCAGCTTGAAGTAGCCGAACGGATCGTCGCGCAGCCC
GGCACCGATGCCTATGGCCGGTTGGCGGTTCTGGCCCAGTGGCGCGCCACCCCGCGCATCGCGACCCGGGTACACCGCAG
CGCCTTCACCCCGCCACCCAAGGTGATGTCGGCCATCATCCACGTAGAACCCGCCGCGATGCCCGAAGGCGTTTCGGCGC
GCATGCTCGAACGGGTGACCGAGGCGGCTTTCGGCCAGCGCCGCAAGATGCTGCGCCAATCGCTAAAAGGCTTGCCCGGT
GCGCTGGATGCGCTCGAAACGCTCGGCATCGATCCGCAACGCCGCGCCGAAACGCTCAGCGTCGAAGATTTCGTGGCGAT
CGCGCGGCTGCTGACGAAGTAG

Upstream 100 bases:

>100_bases
GCACCGCGTTCGCCATCGCCGGCACGGGCACCGCACGCGTCGGTCCGACAATCGCGGCGATCCGGATGGCAGGCGAATGC
GCCAAACGGAGGGCCATGCC

Downstream 100 bases:

>100_bases
GCAGCCGCGCTCCGTCGGCGGCTCAGGCCGGTTCGGCCACCTTCCCCACCGCCGCCTTCTTCTTCAGGCGCCACGCGTGC
AGCAGCGGCTCGGTATAGCC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTDLPPLRDVVNRHGLYATKALGQNFLFDEQLLDRIARVPGKLKGENVLEVGPGPGGLTRALLRAGAKVTAIEMDKRCLP
ALAELADAFPGQLTVIEGDATKIAPETLFDGPWHVAANLPYNVGTQLFTGWLSGQDWPPQWKSLTLMFQLEVAERIVAQP
GTDAYGRLAVLAQWRATPRIATRVHRSAFTPPPKVMSAIIHVEPAAMPEGVSARMLERVTEAAFGQRRKMLRQSLKGLPG
ALDALETLGIDPQRRAETLSVEDFVAIARLLTK

Sequences:

>Translated_273_residues
MTDLPPLRDVVNRHGLYATKALGQNFLFDEQLLDRIARVPGKLKGENVLEVGPGPGGLTRALLRAGAKVTAIEMDKRCLP
ALAELADAFPGQLTVIEGDATKIAPETLFDGPWHVAANLPYNVGTQLFTGWLSGQDWPPQWKSLTLMFQLEVAERIVAQP
GTDAYGRLAVLAQWRATPRIATRVHRSAFTPPPKVMSAIIHVEPAAMPEGVSARMLERVTEAAFGQRRKMLRQSLKGLPG
ALDALETLGIDPQRRAETLSVEDFVAIARLLTK
>Mature_272_residues
TDLPPLRDVVNRHGLYATKALGQNFLFDEQLLDRIARVPGKLKGENVLEVGPGPGGLTRALLRAGAKVTAIEMDKRCLPA
LAELADAFPGQLTVIEGDATKIAPETLFDGPWHVAANLPYNVGTQLFTGWLSGQDWPPQWKSLTLMFQLEVAERIVAQPG
TDAYGRLAVLAQWRATPRIATRVHRSAFTPPPKVMSAIIHVEPAAMPEGVSARMLERVTEAAFGQRRKMLRQSLKGLPGA
LDALETLGIDPQRRAETLSVEDFVAIARLLTK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=295, Percent_Identity=35.5932203389831, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI7657198, Length=227, Percent_Identity=30.8370044052863, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=37.9699248120301, Blast_Score=150, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=31.8791946308725, Blast_Score=143, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI25146882, Length=212, Percent_Identity=33.0188679245283, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357273, Length=246, Percent_Identity=32.9268292682927, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21358017, Length=238, Percent_Identity=32.7731092436975, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NOVAD (Q2G9Z2)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496165.1
- ProteinModelPortal:   Q2G9Z2
- SMR:   Q2G9Z2
- STRING:   Q2G9Z2
- GeneID:   3917972
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0886
- NMPDR:   fig|48935.1.peg.1569
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q2G9Z2
- ProtClustDB:   PRK00274
- BioCyc:   NARO279238:SARO_0886-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 29810; Mature: 29679

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 25-25 BINDING 27-27 BINDING 52-52 BINDING 73-73 BINDING 99-99 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPPLRDVVNRHGLYATKALGQNFLFDEQLLDRIARVPGKLKGENVLEVGPGPGGLTR
CCCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHH
ALLRAGAKVTAIEMDKRCLPALAELADAFPGQLTVIEGDATKIAPETLFDGPWHVAANLP
HHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEECCC
YNVGTQLFTGWLSGQDWPPQWKSLTLMFQLEVAERIVAQPGTDAYGRLAVLAQWRATPRI
HHHHHHHHHHHHCCCCCCCCHHHEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHH
ATRVHRSAFTPPPKVMSAIIHVEPAAMPEGVSARMLERVTEAAFGQRRKMLRQSLKGLPG
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ALDALETLGIDPQRRAETLSVEDFVAIARLLTK
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLPPLRDVVNRHGLYATKALGQNFLFDEQLLDRIARVPGKLKGENVLEVGPGPGGLTR
CCCCHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHH
ALLRAGAKVTAIEMDKRCLPALAELADAFPGQLTVIEGDATKIAPETLFDGPWHVAANLP
HHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEECCC
YNVGTQLFTGWLSGQDWPPQWKSLTLMFQLEVAERIVAQPGTDAYGRLAVLAQWRATPRI
HHHHHHHHHHHHCCCCCCCCHHHEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHH
ATRVHRSAFTPPPKVMSAIIHVEPAAMPEGVSARMLERVTEAAFGQRRKMLRQSLKGLPG
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ALDALETLGIDPQRRAETLSVEDFVAIARLLTK
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA