Click here to switch to the map view.

The map label for this gene is lcfB [H]

Identifier: 87198890

GI number: 87198890

Start: 921894

End: 923498

Strand: Reverse

Name: lcfB [H]

Synonym: Saro_0868

Alternate gene names: 87198890

Gene position: 923498-921894 (Counterclockwise)

Preceding gene: 87198891

Following gene: 87198889

Centisome position: 25.93

GC content: 66.36

Gene sequence:

>1605_bases
ATGCGGATCGAGGATATCGTCGAAAGCGAATTCACGCCGCTGAGCGACATAATCCGCGCCCATGCCGCAAGGCGGGGCGC
GGCAGTGGCGCTGGCGGACGAGAACCGCACTGTCGGCTGGGCGGAAATGGATGCCCTGCTCGACCGCATCGCCGTGGCCC
TCCAGCGCGAGGGCGTGGAAAAGGATGAGCCGGTCGCCATAGCGGCAATGAACTCGGTGACGACGGGGCTGGCCTTCCTT
GGGGCGATCCGTGCCGGGGCGGTTGCGGCACCCCTCACCTCCACTGCGGCGCCCGACACCATCGTGGCCATGCTGGAGGA
CAGCGGCAGCCGGGTGCTGCTGCTCGACAAGGACATTGCCGAGCGGCTTGAAGGGCTGGACCTGCCGGCCGGCGTGAAGC
GCATCGCGCTGGATGACAGCGACGCGGGCGAGCCGTTCAGCTCGTGGATGGCGGAAGAAGGTGCGAAGCCCGAGGAGCGC
GCCGTGGCGCCGGGCGATCCGTTCAACATCATCTATTCGTCGGGCACCACCGGCGCGCCCAAGGGCATCGTGCAGAGCCA
CAAGATGCGCCACGAATACGCGCGGCGCACGATCGTGGCGGGTTACGACGACGATACGGCGGCGATCTGTTCGACGCCGC
TCTATTCCAACACCACGCTGGTCAGCTTCCTTCCGGCGCTCACCGCCGGCGGCAAGGTGGTGCTGATGCGCAAGTTCGAC
GCGCGCACGTTCCTGGAACTGTCCGAGCGCGAGCGGGTGACCAACGCCATGCTGGTGCCGGTGCAGTACCGCCGCATCAT
GGAACTGCCCGACTTCGACAGCTTCGACCTTTCGTCCTACCGGCTGAAGTCCTGCACGTCCGCGCCCTTCCCGGCTTGGC
TGAAGGCCGATGTGCTGAAGCGCTGGCCGGGCGGGCTGACCGATGGCTATGGCATGACCGAAGGCGGGGCGACGACGATC
CTGCTGGCGCACAAGTTCCCGGACAAGCTGCACACCGTGGGCCAGGTCGCACCCGGGCACGTGATGAAGATCATCGACGA
GGACGGGAACGAGTTGCCGCAGGGCGAAGTCGGCGAGATCGTCGGCCATTCGCCGATCATGATGAACGGCTACCACGGCA
AGCCCGACAAGACCCGCGAGATCGAGTGGCATGACAGCGAAGGCCGTCGCTACATCCGCCACGGCGACATCGGCAAGTTC
GACGAGGACGGCTTCCTGATCCTCATGGACCGCGCCAAGGACATGATCATCTCGGGCGGGTTCAACATCTACCCGAGCGA
TCTGGAAGCCGAACTCACCCGGCAAGGCGCGGTGAAGGAAGCGGCCGTGGTCGGCGTGCCCAGCGATGCCTGGGGCGAAA
CCCCGCTTGCGGTGGTGGTGCTGGCCGATCCGGCCGCCGATCCGGCGGCGATCCTTGCCGAGGCGAACAGCCGGCTGGGC
AAGACGCAGCGCATCTCGGCCATCGAAGTGATCGACGAACTGCCCCGCAGCCCCATCGGCAAGGTGCTCAAGCGCGAACT
GCGCGACAAATTCGGCGGGAATCCGATCCTCGCCGCGACCTCCCTGGAAAAGGCCCCTGCCCCAACACGTTCTCAAGGAG
CATGA

Upstream 100 bases:

>100_bases
CCGTGGTTGCGGGCCTGAAGAAGCACGGGCTGCCGGTGGCACCTCTGCTGGAGCGCAAGGCGGCCGCGGGCGAAACGTTC
AACTGAACCGGAGAACGGTG

Downstream 100 bases:

>100_bases
ACATGCGTGACGCCGTAATCGTATCCGCCGCTCGCACGCCTATCGGGCGCGCCTACAAGGGCGCCTTCAATGCCACTCCC
GGCGCGACGCTGGGCGCGCT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MRIEDIVESEFTPLSDIIRAHAARRGAAVALADENRTVGWAEMDALLDRIAVALQREGVEKDEPVAIAAMNSVTTGLAFL
GAIRAGAVAAPLTSTAAPDTIVAMLEDSGSRVLLLDKDIAERLEGLDLPAGVKRIALDDSDAGEPFSSWMAEEGAKPEER
AVAPGDPFNIIYSSGTTGAPKGIVQSHKMRHEYARRTIVAGYDDDTAAICSTPLYSNTTLVSFLPALTAGGKVVLMRKFD
ARTFLELSERERVTNAMLVPVQYRRIMELPDFDSFDLSSYRLKSCTSAPFPAWLKADVLKRWPGGLTDGYGMTEGGATTI
LLAHKFPDKLHTVGQVAPGHVMKIIDEDGNELPQGEVGEIVGHSPIMMNGYHGKPDKTREIEWHDSEGRRYIRHGDIGKF
DEDGFLILMDRAKDMIISGGFNIYPSDLEAELTRQGAVKEAAVVGVPSDAWGETPLAVVVLADPAADPAAILAEANSRLG
KTQRISAIEVIDELPRSPIGKVLKRELRDKFGGNPILAATSLEKAPAPTRSQGA

Sequences:

>Translated_534_residues
MRIEDIVESEFTPLSDIIRAHAARRGAAVALADENRTVGWAEMDALLDRIAVALQREGVEKDEPVAIAAMNSVTTGLAFL
GAIRAGAVAAPLTSTAAPDTIVAMLEDSGSRVLLLDKDIAERLEGLDLPAGVKRIALDDSDAGEPFSSWMAEEGAKPEER
AVAPGDPFNIIYSSGTTGAPKGIVQSHKMRHEYARRTIVAGYDDDTAAICSTPLYSNTTLVSFLPALTAGGKVVLMRKFD
ARTFLELSERERVTNAMLVPVQYRRIMELPDFDSFDLSSYRLKSCTSAPFPAWLKADVLKRWPGGLTDGYGMTEGGATTI
LLAHKFPDKLHTVGQVAPGHVMKIIDEDGNELPQGEVGEIVGHSPIMMNGYHGKPDKTREIEWHDSEGRRYIRHGDIGKF
DEDGFLILMDRAKDMIISGGFNIYPSDLEAELTRQGAVKEAAVVGVPSDAWGETPLAVVVLADPAADPAAILAEANSRLG
KTQRISAIEVIDELPRSPIGKVLKRELRDKFGGNPILAATSLEKAPAPTRSQGA
>Mature_534_residues
MRIEDIVESEFTPLSDIIRAHAARRGAAVALADENRTVGWAEMDALLDRIAVALQREGVEKDEPVAIAAMNSVTTGLAFL
GAIRAGAVAAPLTSTAAPDTIVAMLEDSGSRVLLLDKDIAERLEGLDLPAGVKRIALDDSDAGEPFSSWMAEEGAKPEER
AVAPGDPFNIIYSSGTTGAPKGIVQSHKMRHEYARRTIVAGYDDDTAAICSTPLYSNTTLVSFLPALTAGGKVVLMRKFD
ARTFLELSERERVTNAMLVPVQYRRIMELPDFDSFDLSSYRLKSCTSAPFPAWLKADVLKRWPGGLTDGYGMTEGGATTI
LLAHKFPDKLHTVGQVAPGHVMKIIDEDGNELPQGEVGEIVGHSPIMMNGYHGKPDKTREIEWHDSEGRRYIRHGDIGKF
DEDGFLILMDRAKDMIISGGFNIYPSDLEAELTRQGAVKEAAVVGVPSDAWGETPLAVVVLADPAADPAAILAEANSRLG
KTQRISAIEVIDELPRSPIGKVLKRELRDKFGGNPILAATSLEKAPAPTRSQGA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=533, Percent_Identity=25.703564727955, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI58082049, Length=485, Percent_Identity=27.4226804123711, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157311624, Length=485, Percent_Identity=26.8041237113402, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI157311622, Length=485, Percent_Identity=26.8041237113402, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI38505220, Length=471, Percent_Identity=25.6900212314225, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI115511026, Length=506, Percent_Identity=25.098814229249, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI42544132, Length=469, Percent_Identity=26.226012793177, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI122937307, Length=509, Percent_Identity=27.1119842829077, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI187761345, Length=544, Percent_Identity=24.8161764705882, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI187761343, Length=544, Percent_Identity=24.8161764705882, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI28416953, Length=511, Percent_Identity=24.6575342465753, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI195947378, Length=351, Percent_Identity=24.5014245014245, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI8923896, Length=542, Percent_Identity=22.140221402214, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI253970489, Length=381, Percent_Identity=25.4593175853018, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=25.5474452554745, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=26.2548262548263, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI221142682, Length=486, Percent_Identity=23.8683127572016, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI145693145, Length=496, Percent_Identity=24.7983870967742, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1790505, Length=534, Percent_Identity=22.8464419475655, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786801, Length=475, Percent_Identity=24.2105263157895, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=508, Percent_Identity=28.5433070866142, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=26.1278195488722, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=360, Percent_Identity=28.8888888888889, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=26.3366336633663, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=501, Percent_Identity=25.3493013972056, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=523, Percent_Identity=25.0478011472275, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=372, Percent_Identity=31.4516129032258, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=365, Percent_Identity=28.2191780821918, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=365, Percent_Identity=28.2191780821918, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71994703, Length=365, Percent_Identity=28.2191780821918, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=505, Percent_Identity=26.9306930693069, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=523, Percent_Identity=23.9005736137667, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71985884, Length=394, Percent_Identity=27.1573604060914, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319699, Length=491, Percent_Identity=24.4399185336049, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323182, Length=541, Percent_Identity=23.6598890942699, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355181, Length=487, Percent_Identity=26.694045174538, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21356947, Length=475, Percent_Identity=26.5263157894737, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI18859661, Length=521, Percent_Identity=26.4875239923225, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24648253, Length=499, Percent_Identity=27.0541082164329, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648255, Length=499, Percent_Identity=26.8537074148297, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24648260, Length=487, Percent_Identity=25.4620123203285, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24653035, Length=477, Percent_Identity=24.5283018867925, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356441, Length=517, Percent_Identity=24.9516441005803, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161076582, Length=484, Percent_Identity=23.7603305785124, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24581924, Length=548, Percent_Identity=24.0875912408759, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24656500, Length=479, Percent_Identity=23.7995824634656, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20130357, Length=503, Percent_Identity=24.0556660039761, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=481, Percent_Identity=25.3638253638254, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281365686, Length=281, Percent_Identity=29.5373665480427, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648257, Length=479, Percent_Identity=24.0083507306889, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358303, Length=466, Percent_Identity=22.9613733905579, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355225, Length=559, Percent_Identity=21.6457960644007, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57559; Mature: 57559

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEDIVESEFTPLSDIIRAHAARRGAAVALADENRTVGWAEMDALLDRIAVALQREGVE
CCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
KDEPVAIAAMNSVTTGLAFLGAIRAGAVAAPLTSTAAPDTIVAMLEDSGSRVLLLDKDIA
CCCCEEEEEHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEEECCCCEEEEECHHHH
ERLEGLDLPAGVKRIALDDSDAGEPFSSWMAEEGAKPEERAVAPGDPFNIIYSSGTTGAP
HHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCCC
KGIVQSHKMRHEYARRTIVAGYDDDTAAICSTPLYSNTTLVSFLPALTAGGKVVLMRKFD
HHHHHHHHHHHHHHHCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEECC
ARTFLELSERERVTNAMLVPVQYRRIMELPDFDSFDLSSYRLKSCTSAPFPAWLKADVLK
CHHHHHHHHHHHHHHEEECCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
RWPGGLTDGYGMTEGGATTILLAHKFPDKLHTVGQVAPGHVMKIIDEDGNELPQGEVGEI
HCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHH
VGHSPIMMNGYHGKPDKTREIEWHDSEGRRYIRHGDIGKFDEDGFLILMDRAKDMIISGG
HCCCCEEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECC
FNIYPSDLEAELTRQGAVKEAAVVGVPSDAWGETPLAVVVLADPAADPAAILAEANSRLG
CEECCCCHHHHHHHCCCCHHEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
KTQRISAIEVIDELPRSPIGKVLKRELRDKFGGNPILAATSLEKAPAPTRSQGA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIEDIVESEFTPLSDIIRAHAARRGAAVALADENRTVGWAEMDALLDRIAVALQREGVE
CCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
KDEPVAIAAMNSVTTGLAFLGAIRAGAVAAPLTSTAAPDTIVAMLEDSGSRVLLLDKDIA
CCCCEEEEEHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCEEEEEEECCCCEEEEECHHHH
ERLEGLDLPAGVKRIALDDSDAGEPFSSWMAEEGAKPEERAVAPGDPFNIIYSSGTTGAP
HHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCEEEEECCCCCCC
KGIVQSHKMRHEYARRTIVAGYDDDTAAICSTPLYSNTTLVSFLPALTAGGKVVLMRKFD
HHHHHHHHHHHHHHHCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEEECC
ARTFLELSERERVTNAMLVPVQYRRIMELPDFDSFDLSSYRLKSCTSAPFPAWLKADVLK
CHHHHHHHHHHHHHHEEECCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHH
RWPGGLTDGYGMTEGGATTILLAHKFPDKLHTVGQVAPGHVMKIIDEDGNELPQGEVGEI
HCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHH
VGHSPIMMNGYHGKPDKTREIEWHDSEGRRYIRHGDIGKFDEDGFLILMDRAKDMIISGG
HCCCCEEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCCEEEECC
FNIYPSDLEAELTRQGAVKEAAVVGVPSDAWGETPLAVVVLADPAADPAAILAEANSRLG
CEECCCCHHHHHHHCCCCHHEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
KTQRISAIEVIDELPRSPIGKVLKRELRDKFGGNPILAATSLEKAPAPTRSQGA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]