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The map label for this gene is fadD21 [H]

Identifier: 87198843

GI number: 87198843

Start: 875100

End: 876857

Strand: Reverse

Name: fadD21 [H]

Synonym: Saro_0821

Alternate gene names: 87198843

Gene position: 876857-875100 (Counterclockwise)

Preceding gene: 87198848

Following gene: 87198842

Centisome position: 24.62

GC content: 66.72

Gene sequence:

>1758_bases
ATGACCGTTTCGGACGCCGTCGCTGACAAGGCGACTCTCGTTCCCACGCCCAACCTCGACGTGCAGACGCGTCGATATGC
GGACTTCGACACCTTCTGCGATGCACTCGATTATGCCGCGAAGGGCGTCCTGGGCTTCAACTTCCACGACCCGCGCGGCA
ACCTTGCCCGGGTCTATCCCTATTCCGAGCTGCGCGAGGACGCGCTCGTGGCCGCGCGCAAGCTGATCGGCCTCGGCGTC
CAGCCCGGAGACCGGGTCGCGCTCGTGGCAGAGACGGGCCCCGATTTCGCGGCCATCTTCTGCGGTGCGATGTATGCCGG
CGCATGGCCGGTCCCGCTCCCGCTGCCGACCAGCTTCGGCGGCAAGGAGAGCTACATCGACCAGCTCGCGGTCCAGCTTT
CCAGTTCAGACCCGAAGATGCTCGTCTACCCCGGCGAAATCGCCGAGATGGCCAGTGCCGCAGCCGCACGCCAGGGTTGC
GCGGGCCTGTCGTGGGATGAATTCCACGCCGGCGCAGCCGCCGATGGCCCGCTGCCCACGCCCGATCCGGACGCGATCAG
CTATCTCCAGTATTCGAGCGGGTCGACCCGCTTCCCGCATGGCGTCGCCGTCACTCACCGCGCGCTCATGGCGAACCTCG
CCGGCCATTCGCATGGCATGAAGATCGCGGACCCCGATCGCGCGATCTCGTGGCTGCCCTGGTATCACGACATGGGGCTC
GTCGGCTGCTTCCTCTCGCCGATCGCCAATGGCGTGTCGGTCGATTACCTCAAGACCGAGGACTTCGCCCGCCGCCCGCT
CGCCTGGCTCGACCTGATCAGCCGCAACACCGGCACGACACTCTCCTATTCGCCCACCTTCGGCTACGACATCTGCGCCC
GCCGCATCTCGAGCCAGAGCCACGTTTCCGACCGCTTCGACCTTTCGCGCTGGCGCGTCGCCGGCAACGGGGCCGACATG
ATCCGCCCCGACGTGATGCAGAACTTCGTCAACGCCTTTGCCGAGGCCGGGTTCAAGGCCGAAGCCTTCCTGCCCAGCTA
CGGACTTGCCGAAGCGACGCTTGCCGTCACCATCATGCCCCCGGGCGAAGGCATCCGCGTCGAACTGGTCGAGGAAGAAC
GCCTCTCGGGCACCCCGCGCGACCTTTCGCGCCCGGCCCGCTACCGCGCCATGGTCAACTGCGGCGTTCCCGTGCGCGGC
ATGGAAGTCGCCATTCGTGGCGAGAACGGCAACGAGATCGGCCACCACCACATCGGCAAGGTGTGGTGCCGCGGCTCATC
GGTCATGCACTCCTACTTCCGCGATCCCGAAGCGACCGACGCCTGCATGGTCGACGGCTGGCTCGACACCGGCGACATGG
GCTACGTCGACGAGAACGGCTACCTGTTCATCGTCGGCCGCGCCAAGGACATGATCATCATCAACGGCAAGAACCACTGG
CCCCAGGACATCGAATGGGCGGTGGAGCAGCTTCCCGGCTTCCACCAGGGCGATATCGCCGCCTTCTCGGTCGAGACCGA
GAACGGCGAGGAGGCGCCTGCCGTCCTCGTCCATTGCCGCGTCTCCGACCCGGTCGAACGGGTCAAGCTGCGCGACCAGA
TCCGCGACAAGGTTCGCTCGATCACCGGCATGAACTGCGTCGTGGAACTCGTGCCGCCGCGCACCCTGCCGCGCACCAGC
TCGGGCAAGCTGAGCCGGGCCAAGGCCAAGAAGCTCTACCTCACCGGCGAGATCGCGCCCTATCAGCTCGCAGCCTGA

Upstream 100 bases:

>100_bases
GTGGCAGCATAGATTTCGCGCCGCGGACCAGTCATGGGCCATTCACCTGCGCGCGCAGAATGGGCTCGCAGGACGATAGT
AGAGAAAATCGGGGTATCGC

Downstream 100 bases:

>100_bases
CGTCACGACGTCTCAATTCGGTTTCGTTTCCGGTTAGATACGGGTTGACGCGCCGTTCCTCTTGCCATTCCCTGTGCCAG
GGGAATTGGGGAGAGACGGA

Product: acyl-CoA synthetase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTVSDAVADKATLVPTPNLDVQTRRYADFDTFCDALDYAAKGVLGFNFHDPRGNLARVYPYSELREDALVAARKLIGLGV
QPGDRVALVAETGPDFAAIFCGAMYAGAWPVPLPLPTSFGGKESYIDQLAVQLSSSDPKMLVYPGEIAEMASAAAARQGC
AGLSWDEFHAGAAADGPLPTPDPDAISYLQYSSGSTRFPHGVAVTHRALMANLAGHSHGMKIADPDRAISWLPWYHDMGL
VGCFLSPIANGVSVDYLKTEDFARRPLAWLDLISRNTGTTLSYSPTFGYDICARRISSQSHVSDRFDLSRWRVAGNGADM
IRPDVMQNFVNAFAEAGFKAEAFLPSYGLAEATLAVTIMPPGEGIRVELVEEERLSGTPRDLSRPARYRAMVNCGVPVRG
MEVAIRGENGNEIGHHHIGKVWCRGSSVMHSYFRDPEATDACMVDGWLDTGDMGYVDENGYLFIVGRAKDMIIINGKNHW
PQDIEWAVEQLPGFHQGDIAAFSVETENGEEAPAVLVHCRVSDPVERVKLRDQIRDKVRSITGMNCVVELVPPRTLPRTS
SGKLSRAKAKKLYLTGEIAPYQLAA

Sequences:

>Translated_585_residues
MTVSDAVADKATLVPTPNLDVQTRRYADFDTFCDALDYAAKGVLGFNFHDPRGNLARVYPYSELREDALVAARKLIGLGV
QPGDRVALVAETGPDFAAIFCGAMYAGAWPVPLPLPTSFGGKESYIDQLAVQLSSSDPKMLVYPGEIAEMASAAAARQGC
AGLSWDEFHAGAAADGPLPTPDPDAISYLQYSSGSTRFPHGVAVTHRALMANLAGHSHGMKIADPDRAISWLPWYHDMGL
VGCFLSPIANGVSVDYLKTEDFARRPLAWLDLISRNTGTTLSYSPTFGYDICARRISSQSHVSDRFDLSRWRVAGNGADM
IRPDVMQNFVNAFAEAGFKAEAFLPSYGLAEATLAVTIMPPGEGIRVELVEEERLSGTPRDLSRPARYRAMVNCGVPVRG
MEVAIRGENGNEIGHHHIGKVWCRGSSVMHSYFRDPEATDACMVDGWLDTGDMGYVDENGYLFIVGRAKDMIIINGKNHW
PQDIEWAVEQLPGFHQGDIAAFSVETENGEEAPAVLVHCRVSDPVERVKLRDQIRDKVRSITGMNCVVELVPPRTLPRTS
SGKLSRAKAKKLYLTGEIAPYQLAA
>Mature_584_residues
TVSDAVADKATLVPTPNLDVQTRRYADFDTFCDALDYAAKGVLGFNFHDPRGNLARVYPYSELREDALVAARKLIGLGVQ
PGDRVALVAETGPDFAAIFCGAMYAGAWPVPLPLPTSFGGKESYIDQLAVQLSSSDPKMLVYPGEIAEMASAAAARQGCA
GLSWDEFHAGAAADGPLPTPDPDAISYLQYSSGSTRFPHGVAVTHRALMANLAGHSHGMKIADPDRAISWLPWYHDMGLV
GCFLSPIANGVSVDYLKTEDFARRPLAWLDLISRNTGTTLSYSPTFGYDICARRISSQSHVSDRFDLSRWRVAGNGADMI
RPDVMQNFVNAFAEAGFKAEAFLPSYGLAEATLAVTIMPPGEGIRVELVEEERLSGTPRDLSRPARYRAMVNCGVPVRGM
EVAIRGENGNEIGHHHIGKVWCRGSSVMHSYFRDPEATDACMVDGWLDTGDMGYVDENGYLFIVGRAKDMIIINGKNHWP
QDIEWAVEQLPGFHQGDIAAFSVETENGEEAPAVLVHCRVSDPVERVKLRDQIRDKVRSITGMNCVVELVPPRTLPRTSS
GKLSRAKAKKLYLTGEIAPYQLAA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=493, Percent_Identity=23.9350912778905, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788107, Length=555, Percent_Identity=24.5045045045045, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI145693145, Length=537, Percent_Identity=22.9050279329609, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=593, Percent_Identity=22.9342327150084, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=24.1779497098646, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=443, Percent_Identity=24.3792325056433, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193202679, Length=569, Percent_Identity=21.9683655536028, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=522, Percent_Identity=22.6053639846743, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=21.9378427787934, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=546, Percent_Identity=20.3296703296703, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=479, Percent_Identity=24.0083507306889, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18859661, Length=559, Percent_Identity=22.0035778175313, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20130357, Length=175, Percent_Identity=27.4285714285714, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356947, Length=182, Percent_Identity=28.021978021978, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356441, Length=537, Percent_Identity=22.7188081936685, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161076582, Length=184, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=22.3230490018149, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21358303, Length=192, Percent_Identity=29.6875, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648260, Length=181, Percent_Identity=30.3867403314917, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281365686, Length=187, Percent_Identity=27.807486631016, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=191, Percent_Identity=27.2251308900524, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24654656, Length=611, Percent_Identity=21.7675941080196, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648257, Length=177, Percent_Identity=29.9435028248588, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648255, Length=201, Percent_Identity=26.3681592039801, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648253, Length=201, Percent_Identity=26.3681592039801, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 63742; Mature: 63611

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSDAVADKATLVPTPNLDVQTRRYADFDTFCDALDYAAKGVLGFNFHDPRGNLARVYP
CCCCHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCEECCCCCEEEECC
YSELREDALVAARKLIGLGVQPGDRVALVAETGPDFAAIFCGAMYAGAWPVPLPLPTSFG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GKESYIDQLAVQLSSSDPKMLVYPGEIAEMASAAAARQGCAGLSWDEFHAGAAADGPLPT
CHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC
PDPDAISYLQYSSGSTRFPHGVAVTHRALMANLAGHSHGMKIADPDRAISWLPWYHDMGL
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCEEECCCHHHHHH
VGCFLSPIANGVSVDYLKTEDFARRPLAWLDLISRNTGTTLSYSPTFGYDICARRISSQS
HHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHH
HVSDRFDLSRWRVAGNGADMIRPDVMQNFVNAFAEAGFKAEAFLPSYGLAEATLAVTIMP
HCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEC
PGEGIRVELVEEERLSGTPRDLSRPARYRAMVNCGVPVRGMEVAIRGENGNEIGHHHIGK
CCCCCEEEEEEHHHCCCCCHHHCCCHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
VWCRGSSVMHSYFRDPEATDACMVDGWLDTGDMGYVDENGYLFIVGRAKDMIIINGKNHW
EEECCHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCC
PQDIEWAVEQLPGFHQGDIAAFSVETENGEEAPAVLVHCRVSDPVERVKLRDQIRDKVRS
CHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
ITGMNCVVELVPPRTLPRTSSGKLSRAKAKKLYLTGEIAPYQLAA
HCCCCEEEEECCCCCCCCCCCCCCHHHCCEEEEEECCCCCCEECC
>Mature Secondary Structure 
TVSDAVADKATLVPTPNLDVQTRRYADFDTFCDALDYAAKGVLGFNFHDPRGNLARVYP
CCCHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCEECCCCCEEEECC
YSELREDALVAARKLIGLGVQPGDRVALVAETGPDFAAIFCGAMYAGAWPVPLPLPTSFG
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GKESYIDQLAVQLSSSDPKMLVYPGEIAEMASAAAARQGCAGLSWDEFHAGAAADGPLPT
CHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC
PDPDAISYLQYSSGSTRFPHGVAVTHRALMANLAGHSHGMKIADPDRAISWLPWYHDMGL
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCEEECCCHHHHHH
VGCFLSPIANGVSVDYLKTEDFARRPLAWLDLISRNTGTTLSYSPTFGYDICARRISSQS
HHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCHH
HVSDRFDLSRWRVAGNGADMIRPDVMQNFVNAFAEAGFKAEAFLPSYGLAEATLAVTIMP
HCHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEEC
PGEGIRVELVEEERLSGTPRDLSRPARYRAMVNCGVPVRGMEVAIRGENGNEIGHHHIGK
CCCCCEEEEEEHHHCCCCCHHHCCCHHEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCC
VWCRGSSVMHSYFRDPEATDACMVDGWLDTGDMGYVDENGYLFIVGRAKDMIIINGKNHW
EEECCHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEECCCEEEEEECCCCEEEECCCCCC
PQDIEWAVEQLPGFHQGDIAAFSVETENGEEAPAVLVHCRVSDPVERVKLRDQIRDKVRS
CHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
ITGMNCVVELVPPRTLPRTSSGKLSRAKAKKLYLTGEIAPYQLAA
HCCCCEEEEECCCCCCCCCCCCCCHHHCCEEEEEECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]