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The map label for this gene is bioB

Identifier: 87198743

GI number: 87198743

Start: 758713

End: 759783

Strand: Reverse

Name: bioB

Synonym: Saro_0719

Alternate gene names: 87198743

Gene position: 759783-758713 (Counterclockwise)

Preceding gene: 87198744

Following gene: 87198742

Centisome position: 21.33

GC content: 64.89

Gene sequence:

>1071_bases
GTGCAGGAAACCGCGCTTCTTGAGCGAGAATCGGCTGGAACCGGCAACGCCCCCGTCCGCACCGACTGGACGCGCGAGGA
AATCGCCGCGCTGTTCGACCTGCCATTCACCGAACTCCTGTTCCGGGCTGCCGAGGTACACCGCGCGCACCATCGCGCAG
GCGAGGTGCAGCTCTGCACGCTGCTGTCGATCAAGACCGGCGGGTGCCCTGAAGATTGCGGCTACTGTTCGCAATCGGTG
AAGGCAGACAGCGGCGTCGAGGCGACGAAACTGATGGAAGTGCAGAAGGTCCTGCAATCCGCCGCGCAGGCCAAGGACAA
TGGCTCCAAGCGCTTCTGCATGGGCGCCGCCTGGCGCAACCCCAAGGACCGAGACATGCCGGCGATCATCTCCATGATCA
AGGGCGTGCGCGAGATGGGCATGGAAACCTGCATGACGCTTGGCATGCTGACGCCTTCGCAGGCAGCGCAGCTCGCCGAC
GCGGGTCTCGACTACTACAACCATAACATCGACACCTCGCCCGAACGCTACGAGGAAGTCATCACCACCCGCACTTTTGC
GGACCGCCTGCAGACGCTCGACAACGTGCGCAACGCCGGGATCAACGTGTGCTCGGGCGGCATCGTCGGCATGGGCGAGA
CTCGCGCCGACCGCGTCGGCTTCGTTCACGCCCTCGCCACGCTTGAACAGCATCCGGAAAGCGTTCCCGTGAACGCCCTC
GTCCCGATCAAGGGCACCGTGCTGGGCGACATGCTGGCCGATACCCCGCTCGCGAAGATCGACGACATCGAGTTCGTTCG
CACCGTGGCCGTCGCGCGCATCACCATGCCGCTCTCTATGGTTCGCCTGTCCGCCGGACGCGAATCGATGTCGGAGGCCA
CGCAGGCGCTGTGCTTCATGGCAGGCGCCAACTCGATCTTCACCGGCGACAAGCTGCTTACCGCTGCCAACGCCGGCGAC
GATGCCGATGCCGCCATGTTCAAGCGCCTCGGTCTCAAGCCGATGGAAGGCGAGGAACCCATGCGGGCGATGAAGTCCGT
CGGAGGCTGCTCCGGAGGCTGCGCGGCGTAA

Upstream 100 bases:

>100_bases
CAAGATTTCGTCACGCGGTTCCCGCTTGAATTCGCAGGCGCGCTGGCCAAAGTTTTACGCTATGGGCCCCGCCTACGTAT
TCACTGGAAGGATTCCGAAC

Downstream 100 bases:

>100_bases
TGGCCGAAAGCACCACCCGCACCGGCAACCTGTGGTCGGCGCACACCGCCGACCTTGCAGCGCTTGGCGAAAAGGCGCGC
CTGCGCTCGCTCGCTCCGCG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQETALLERESAGTGNAPVRTDWTREEIAALFDLPFTELLFRAAEVHRAHHRAGEVQLCTLLSIKTGGCPEDCGYCSQSV
KADSGVEATKLMEVQKVLQSAAQAKDNGSKRFCMGAAWRNPKDRDMPAIISMIKGVREMGMETCMTLGMLTPSQAAQLAD
AGLDYYNHNIDTSPERYEEVITTRTFADRLQTLDNVRNAGINVCSGGIVGMGETRADRVGFVHALATLEQHPESVPVNAL
VPIKGTVLGDMLADTPLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGD
DADAAMFKRLGLKPMEGEEPMRAMKSVGGCSGGCAA

Sequences:

>Translated_356_residues
MQETALLERESAGTGNAPVRTDWTREEIAALFDLPFTELLFRAAEVHRAHHRAGEVQLCTLLSIKTGGCPEDCGYCSQSV
KADSGVEATKLMEVQKVLQSAAQAKDNGSKRFCMGAAWRNPKDRDMPAIISMIKGVREMGMETCMTLGMLTPSQAAQLAD
AGLDYYNHNIDTSPERYEEVITTRTFADRLQTLDNVRNAGINVCSGGIVGMGETRADRVGFVHALATLEQHPESVPVNAL
VPIKGTVLGDMLADTPLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGD
DADAAMFKRLGLKPMEGEEPMRAMKSVGGCSGGCAA
>Mature_356_residues
MQETALLERESAGTGNAPVRTDWTREEIAALFDLPFTELLFRAAEVHRAHHRAGEVQLCTLLSIKTGGCPEDCGYCSQSV
KADSGVEATKLMEVQKVLQSAAQAKDNGSKRFCMGAAWRNPKDRDMPAIISMIKGVREMGMETCMTLGMLTPSQAAQLAD
AGLDYYNHNIDTSPERYEEVITTRTFADRLQTLDNVRNAGINVCSGGIVGMGETRADRVGFVHALATLEQHPESVPVNAL
VPIKGTVLGDMLADTPLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFMAGANSIFTGDKLLTAANAGD
DADAAMFKRLGLKPMEGEEPMRAMKSVGGCSGGCAA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=317, Percent_Identity=58.6750788643533, Blast_Score=394, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=51.4195583596215, Blast_Score=352, Evalue=5e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_NOVAD (Q2GAF7)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_496000.1
- ProteinModelPortal:   Q2GAF7
- SMR:   Q2GAF7
- STRING:   Q2GAF7
- GeneID:   3918543
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0719
- NMPDR:   fig|48935.1.peg.1627
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q2GAF7
- ProtClustDB:   CLSK2391555
- BioCyc:   NARO279238:SARO_0719-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38170; Mature: 38170

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETALLERESAGTGNAPVRTDWTREEIAALFDLPFTELLFRAAEVHRAHHRAGEVQLCT
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LLSIKTGGCPEDCGYCSQSVKADSGVEATKLMEVQKVLQSAAQAKDNGSKRFCMGAAWRN
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PKDRDMPAIISMIKGVREMGMETCMTLGMLTPSQAAQLADAGLDYYNHNIDTSPERYEEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ITTRTFADRLQTLDNVRNAGINVCSGGIVGMGETRADRVGFVHALATLEQHPESVPVNAL
HHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VPIKGTVLGDMLADTPLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFM
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AGANSIFTGDKLLTAANAGDDADAAMFKRLGLKPMEGEEPMRAMKSVGGCSGGCAA
HCCCCEECCCHHEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQETALLERESAGTGNAPVRTDWTREEIAALFDLPFTELLFRAAEVHRAHHRAGEVQLCT
CCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LLSIKTGGCPEDCGYCSQSVKADSGVEATKLMEVQKVLQSAAQAKDNGSKRFCMGAAWRN
EEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PKDRDMPAIISMIKGVREMGMETCMTLGMLTPSQAAQLADAGLDYYNHNIDTSPERYEEV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ITTRTFADRLQTLDNVRNAGINVCSGGIVGMGETRADRVGFVHALATLEQHPESVPVNAL
HHHHHHHHHHHHHHHHHHCCCEEECCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEE
VPIKGTVLGDMLADTPLAKIDDIEFVRTVAVARITMPLSMVRLSAGRESMSEATQALCFM
ECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AGANSIFTGDKLLTAANAGDDADAAMFKRLGLKPMEGEEPMRAMKSVGGCSGGCAA
HCCCCEECCCHHEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA