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The map label for this gene is gatA [H]

Identifier: 87198629

GI number: 87198629

Start: 649374

End: 651143

Strand: Reverse

Name: gatA [H]

Synonym: Saro_0605

Alternate gene names: 87198629

Gene position: 651143-649374 (Counterclockwise)

Preceding gene: 87198630

Following gene: 87198628

Centisome position: 18.28

GC content: 69.77

Gene sequence:

>1770_bases
ATGACGGCGGCGCCGCGCCCCACCGCCCGCGCCATTGCCGCAGCGGTCAACTCCGGGCAGACCACCGCACTGGCCGTGGC
AGAGGCCACCCTGCTCCGGCTCGCCGCCTACGACGCGGTCCAGCCGCAAATCTGGATCAGCCGCGCCAGTCCCGAAGCCC
TGCTGGCAGCCGCTCGCGCCATCGATGTGCGCCTCGCCGCCGGAGAGGACCTCCCGCTCGCGGGCGTCCCCTTCGCGGTA
AAGGACAACATCGACGTCGCCGGCTTCCACACCACTGCCGCCTGCCCGGCCTTCGCCTATCGGCCCGCAACGTCGGCGAC
GGTTGTGGAGCGCCTGCTCGCGGCGGGCGCGCTCTGCGTCGGCAAGACCAACCTCGATCAGTTCGCGACCGGCCTCAACG
GCACGCGCAGCCCCTATGGCGCCCCGCGCAATGCCCACAACCTCGCCTATGTCAGTGGCGGCTCCAGCTCCGGCTCGGCC
AGCGCGGTGGCGGCCGGGCTCGTCGCCTTCGCCCTCGGCACGGACACCGCAGGGTCCGGGCGCGTACCCGCCGCGTTCCA
GCACCTCGTCGGCTTCAAGCCGAGCAAGGGCCGGTGGAGCAATCGCGGGCTCGTCCCGGCGTGCCGCACGCTCGACTGCA
TAACCGTCTTCGCCCACGACACCGCCGATGCGCGCATTGTCGACGGCATCGTCGCGGGGTTCGATCCGGCCGACGCCTTC
TCCAAACCACTCGCGGACCGACCACGGAAGATGCGCGCCATCGGCGTGCCCCGCCGCGACCAGCGCGCCTTCTTCGGCGA
TGTCGAGGCCGAACATCTCTACGACCGCGCCCTGGACCGGCTTTCGACGCTCGGCCGGATCGTCGAGATCGACTATGCCC
CGCTGCAGGAAGCAGCGCAGCTTCTCTACGGGGGCCCCTGGGTTGCCGAACGGACCGCCGCACTGGCCGGCCTCCTTGCC
GACAATCCCGACGCCCTGGACCCGACCGTCCGGGAAGTCGTGGCGCCCGGGCAGGACATCGGCGCGGTAGACCTGTTCAA
CGGCATCTACCGGCTCGCCGAACTGAAACGACACGCCGACACGCTCTGGGAAAGCATCGACCTGCTGGCCTTTCCCACCA
CGGGCACGACCTATCGGGTGGCGGAATTGCTGGCCGCACCAATCGCACTCAACAGTGCGCTTGGGTACTATACCAACTTC
GTGAACCTGCTCGACATGGCCGCGCTCGCCGTGCCGGCCGGCTCGCGGGCCAACGCGACCGGCTTCGGCGTGACCCTGAT
CGGGCCGGCCGACACCGACCTGGCGCTTCTCGACGCGGCGGAAGCCTATCTGTCCGTGGCAGATCTCCCACCACCACCTC
CGCTCGACCTGGAGGGCAAGATGCAGACCGTGAAACTCGCCGTCGTTGGCGCCCATCTCAAGGACATGCCGCTCCACTGG
CAACTCACCTCGCGCGACGCGAAATTCGTGGGCGCGTTCGAAACCGCCCCCAACTACCGCCTCTACGCCATGGCCGACAG
CGTGCCGCCCAAGCCTGCGCTGATCCATAGCGAGGACGGCGGCGCCATCGCTATCGAGGTCTACGAACTGGGGGTCGCCG
AATTCGGCAGCTTCGTGGCCGAAGTGCCGCCGCCGCTGGCGATCGGCACGGTCACGCTTGCCGATGGCAGCAGCGTCAAG
GGCTTCGTCGCGGAACCCCGCGCCCTCGTCGGCGCGCGGGACATCACCCACCTTGGCGGCTGGCGCGCCTTCGTTGCGGC
GGGAGCATGA

Upstream 100 bases:

>100_bases
GGGTCGAAAGTCCGGGGGCGGGCACTGTCGCCGCGCTCTATGCGCAGGAGCGCCAGTCGGTCCAGCCCGGCACGCCGATG
CTCGCCCTGACGAGGCACGC

Downstream 100 bases:

>100_bases
AGATGCGCAAGCTTTCCATCCCGCTCGCGGTGATCGGCGCAGCCCTCGCGTCGACCCCGCTCCTCGCCGCCCCCGCAAAG
GAGCAGGTCATCGAAGTCCC

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTAAPRPTARAIAAAVNSGQTTALAVAEATLLRLAAYDAVQPQIWISRASPEALLAAARAIDVRLAAGEDLPLAGVPFAV
KDNIDVAGFHTTAACPAFAYRPATSATVVERLLAAGALCVGKTNLDQFATGLNGTRSPYGAPRNAHNLAYVSGGSSSGSA
SAVAAGLVAFALGTDTAGSGRVPAAFQHLVGFKPSKGRWSNRGLVPACRTLDCITVFAHDTADARIVDGIVAGFDPADAF
SKPLADRPRKMRAIGVPRRDQRAFFGDVEAEHLYDRALDRLSTLGRIVEIDYAPLQEAAQLLYGGPWVAERTAALAGLLA
DNPDALDPTVREVVAPGQDIGAVDLFNGIYRLAELKRHADTLWESIDLLAFPTTGTTYRVAELLAAPIALNSALGYYTNF
VNLLDMAALAVPAGSRANATGFGVTLIGPADTDLALLDAAEAYLSVADLPPPPPLDLEGKMQTVKLAVVGAHLKDMPLHW
QLTSRDAKFVGAFETAPNYRLYAMADSVPPKPALIHSEDGGAIAIEVYELGVAEFGSFVAEVPPPLAIGTVTLADGSSVK
GFVAEPRALVGARDITHLGGWRAFVAAGA

Sequences:

>Translated_589_residues
MTAAPRPTARAIAAAVNSGQTTALAVAEATLLRLAAYDAVQPQIWISRASPEALLAAARAIDVRLAAGEDLPLAGVPFAV
KDNIDVAGFHTTAACPAFAYRPATSATVVERLLAAGALCVGKTNLDQFATGLNGTRSPYGAPRNAHNLAYVSGGSSSGSA
SAVAAGLVAFALGTDTAGSGRVPAAFQHLVGFKPSKGRWSNRGLVPACRTLDCITVFAHDTADARIVDGIVAGFDPADAF
SKPLADRPRKMRAIGVPRRDQRAFFGDVEAEHLYDRALDRLSTLGRIVEIDYAPLQEAAQLLYGGPWVAERTAALAGLLA
DNPDALDPTVREVVAPGQDIGAVDLFNGIYRLAELKRHADTLWESIDLLAFPTTGTTYRVAELLAAPIALNSALGYYTNF
VNLLDMAALAVPAGSRANATGFGVTLIGPADTDLALLDAAEAYLSVADLPPPPPLDLEGKMQTVKLAVVGAHLKDMPLHW
QLTSRDAKFVGAFETAPNYRLYAMADSVPPKPALIHSEDGGAIAIEVYELGVAEFGSFVAEVPPPLAIGTVTLADGSSVK
GFVAEPRALVGARDITHLGGWRAFVAAGA
>Mature_588_residues
TAAPRPTARAIAAAVNSGQTTALAVAEATLLRLAAYDAVQPQIWISRASPEALLAAARAIDVRLAAGEDLPLAGVPFAVK
DNIDVAGFHTTAACPAFAYRPATSATVVERLLAAGALCVGKTNLDQFATGLNGTRSPYGAPRNAHNLAYVSGGSSSGSAS
AVAAGLVAFALGTDTAGSGRVPAAFQHLVGFKPSKGRWSNRGLVPACRTLDCITVFAHDTADARIVDGIVAGFDPADAFS
KPLADRPRKMRAIGVPRRDQRAFFGDVEAEHLYDRALDRLSTLGRIVEIDYAPLQEAAQLLYGGPWVAERTAALAGLLAD
NPDALDPTVREVVAPGQDIGAVDLFNGIYRLAELKRHADTLWESIDLLAFPTTGTTYRVAELLAAPIALNSALGYYTNFV
NLLDMAALAVPAGSRANATGFGVTLIGPADTDLALLDAAEAYLSVADLPPPPPLDLEGKMQTVKLAVVGAHLKDMPLHWQ
LTSRDAKFVGAFETAPNYRLYAMADSVPPKPALIHSEDGGAIAIEVYELGVAEFGSFVAEVPPPLAIGTVTLADGSSVKG
FVAEPRALVGARDITHLGGWRAFVAAGA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=27.2727272727273, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI195972892, Length=225, Percent_Identity=28.8888888888889, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=403, Percent_Identity=28.0397022332506, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=186, Percent_Identity=31.7204301075269, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=133, Percent_Identity=35.3383458646617, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=439, Percent_Identity=23.0068337129841, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=128, Percent_Identity=35.15625, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=570, Percent_Identity=43.6842105263158, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=28.3018867924528, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648113, Length=241, Percent_Identity=36.9294605809129, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356731, Length=155, Percent_Identity=35.4838709677419, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922090, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652981, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652983, Length=211, Percent_Identity=29.3838862559242, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648435, Length=208, Percent_Identity=28.8461538461538, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648437, Length=208, Percent_Identity=28.8461538461538, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550774, Length=196, Percent_Identity=29.0816326530612, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 61354; Mature: 61223

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPRPTARAIAAAVNSGQTTALAVAEATLLRLAAYDAVQPQIWISRASPEALLAAARA
CCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
IDVRLAAGEDLPLAGVPFAVKDNIDVAGFHTTAACPAFAYRPATSATVVERLLAAGALCV
HEEEEECCCCCCCCCCCEEEECCCEEEECCHHHCCCCCEECCCCHHHHHHHHHHCCEEEE
GKTNLDQFATGLNGTRSPYGAPRNAHNLAYVSGGSSSGSASAVAAGLVAFALGTDTAGSG
CCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
RVPAAFQHLVGFKPSKGRWSNRGLVPACRTLDCITVFAHDTADARIVDGIVAGFDPADAF
CCCHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCHHHHHHHCCCCCHHHH
SKPLADRPRKMRAIGVPRRDQRAFFGDVEAEHLYDRALDRLSTLGRIVEIDYAPLQEAAQ
HCCHHCCCCHHHEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
LLYGGPWVAERTAALAGLLADNPDALDPTVREVVAPGQDIGAVDLFNGIYRLAELKRHAD
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TLWESIDLLAFPTTGTTYRVAELLAAPIALNSALGYYTNFVNLLDMAALAVPAGSRANAT
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GFGVTLIGPADTDLALLDAAEAYLSVADLPPPPPLDLEGKMQTVKLAVVGAHLKDMPLHW
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCHHCCCEEE
QLTSRDAKFVGAFETAPNYRLYAMADSVPPKPALIHSEDGGAIAIEVYELGVAEFGSFVA
EEECCCCEEEEEEECCCCEEEEEEECCCCCCCCEEECCCCCEEEEEEEECCHHHHHHHHH
EVPPPLAIGTVTLADGSSVKGFVAEPRALVGARDITHLGGWRAFVAAGA
HCCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCEEEEECCC
>Mature Secondary Structure 
TAAPRPTARAIAAAVNSGQTTALAVAEATLLRLAAYDAVQPQIWISRASPEALLAAARA
CCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHH
IDVRLAAGEDLPLAGVPFAVKDNIDVAGFHTTAACPAFAYRPATSATVVERLLAAGALCV
HEEEEECCCCCCCCCCCEEEECCCEEEECCHHHCCCCCEECCCCHHHHHHHHHHCCEEEE
GKTNLDQFATGLNGTRSPYGAPRNAHNLAYVSGGSSSGSASAVAAGLVAFALGTDTAGSG
CCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCC
RVPAAFQHLVGFKPSKGRWSNRGLVPACRTLDCITVFAHDTADARIVDGIVAGFDPADAF
CCCHHHHHHHCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCCHHHHHHHCCCCCHHHH
SKPLADRPRKMRAIGVPRRDQRAFFGDVEAEHLYDRALDRLSTLGRIVEIDYAPLQEAAQ
HCCHHCCCCHHHEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHH
LLYGGPWVAERTAALAGLLADNPDALDPTVREVVAPGQDIGAVDLFNGIYRLAELKRHAD
HHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
TLWESIDLLAFPTTGTTYRVAELLAAPIALNSALGYYTNFVNLLDMAALAVPAGSRANAT
HHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GFGVTLIGPADTDLALLDAAEAYLSVADLPPPPPLDLEGKMQTVKLAVVGAHLKDMPLHW
CCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCHHCCCEEE
QLTSRDAKFVGAFETAPNYRLYAMADSVPPKPALIHSEDGGAIAIEVYELGVAEFGSFVA
EEECCCCEEEEEEECCCCEEEEEEECCCCCCCCEEECCCCCEEEEEEEECCHHHHHHHHH
EVPPPLAIGTVTLADGSSVKGFVAEPRALVGARDITHLGGWRAFVAAGA
HCCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]